• 대한바이러스학회지
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• 제26권1호
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• pp.67-76
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• 1996

The methods that make Hantavirus grow consist of inoculation into the experimental animals and cultured cells. The cultured cells, such as Vero-E6 and A549 cells, have been usually used for isolation of the virus and the animals, such as mice and rats, are used for large scale preparation of the virus so far. Furthermore, the cell can be used to maintain the virus and assay the infectivity and the animals can be used for the experiment of viral pathogenicity and challenge for assessment of vaccine. Apodemus mice, the own natural host of the virus, has been used for challenge test of Hantaan virus. However it has been pointed out to difficult handling and breeding the animal in laboratory. Therefore, we attempted to establish a new animal model for challenge test at the time of isolation of Maaji virus which is a new hantavirus similar but distinct to Hantaan virus. In suckling hamster, the titer of Maaji virus and the lethality to mice of the virus were increased gradually in the titer and lethality through passage by intracerebral (IC) inoculation. We tried to re-adapt this brain virus to lung of weanling hamster. The brain passaged virus was inoculated into weanling hamster intramuscularly. Again, the titer of the virus in lung was also increased by continuous passage of this virus. This facts could regarded as adaptation to new environment in which the virus proliferates. To identity the virus passaged in hamster with Maaji virus, both of the virus passaged in hamster brain and lung were compared with Maaji virus (MAA-I) and Hantaan virus (HTN 76-118) by means of restriction fragment length polymorphism (RFLP) and slingle strand conformation polymophism (SSCP). As a result, we conclude that Maaji virus could be adapted successfully to weanling hamster through this passage strategy. Utilizing this adapted Maaji virus strain, hamster model is able to be used for challenge test in hantaviral vaccinology and further experiments utilizing hamster system as a rather available and convenient lab animal are expected.

• 대한수의학회지
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• 제40권1호
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• pp.110-116
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• 2000

Duck viral hepatitis is an acutic, highly infectious viral disease of young ducklings. The most practical means for controlling duck viral hepatitis is the vaccination of ducklings or of a breeding stock. We attempted to develop a vaccine strain of duck hepatitis virus (DHV) using a Korean isolate by serial chicken embryo passages. The propagation of DHV in chicken embryos was carried 140 passages. After the $50^{th}$ passage, of which the virus was non-pathogenic for ducklings, approximately every $20^{th}$ passage of the virus was tested for vaccinal efficacy. Both the $70^{th}$ and $90^{th}$ passage of the virus gave good protection against challenge infection to a DHV-DRL reference strain(type 1) and a virulent Korean isolate. The $110^{th}$, $125^{th}$ and $140^{th}$ passage of the virus were less protective than the $70^{th}$ and $90^{th}$ passage, which means that more than $110^{th}$ passage may lead to over-attenuation of the virus. Ducklings vaccinated with the chicken-embryo-adapted virus by oral, intramuscular or eye drop administration showed earlier resistance to challenge infection from 3 to 7 days postvaccination. Of the above methods, ducklings vaccinated intramuscularly presented the most rapid resistance against challenge. The minimum immune dose of the chicken-embryo-adapted virus in ducklings was also studied. Ducklings inoculated with a dose of $10^{2.0}\;ELD_{50}$ and below were not fully protected against challenge with a virulent DHV, showing a protection rate of 67% to 73%, but ducklings inoculated with a dose of $10^{3.0}\;ELD_{50}$ and over were completely protected. The virus yield of the chicken-embryo-adapted DHV was examined at 24hrs and 48hrs of the incubation time in the allantoic fluid, embryo head and embryo minus head of the embryonating egg. In all three components, the titer of the virus was higher at 48 hours than that at 24 hours after incubation. And the titer of the virus was higher in the embryo minus head, embryo head and the allantoic fluid, in order. Field trials for evaluating the efficacy of the attenuated DHV as a live vaccine were done in duck farms with about 25% mortality of flocks resulting from duck viral hepatitis. After the use of the experimental vaccine, the mortality due to duck viral hepatitis was dramatically reduced in the farms. These results indicated that the attenuated DHV using a Korean isolate could be a good candidate as a live vaccine strain of DHV in Korea.

• 한국정보통신학회:학술대회논문집
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• 한국정보통신학회 2014년도 추계학술대회
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• pp.661-663
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• 2014

대형 건물에 설치된 조명의 경우, 야간 통행자를 위해 인체 감지센서나 재실 감지센서를 이용하여 통행자가 존재할 때 조명기구를 점등하고 통행자가 없을 시 자동적으로 조명기구를 소등하여 불필요한 전력 소모를 줄인다. 하지만 부적절한 센서 위치로 인한 오작동의 문제가 있으며, 통로의 경우 통행자 통과 후에도 장시간 조명기구가 점등되어 효율적으로 전력 소비를 감소시키지 못한다. 본 논문에서는 실내 위치 측위 기술 중 하나인 fingerprinting 기법을 이용, 통행자의 위치를 추정하여 조명기구를 제어하는 방안을 제안한다. 1개 통로를 대상으로 한 실험 결과 통행자 이탈 후 조명이 점등되어 있는 시간이 기존방식에 비해 약 7분정도 감소하는 것을 확인할 수 있었다.

• 대한조선학회논문집
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• 제59권6호
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• pp.385-392
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• 2022

Naval ship could face a variety of threats in isolated seas. In particular, fires and flooding are defined as disasters that are very likely to cause irreparable damage to ships. These disasters have a very high risk of personal injury as well. Therefore, when a disaster occurs, it must be quickly suppressed, but if there are people in the disaster area, the protection of life must be given priority. In order to quickly evacuate the ship crew in case of a disaster, we would like to propose a plan to quickly explore the evacuation route even in urgent situations. Using commercial escape simulation software, we obtain the data for deep neural network learning with simulations according to aisle characteristics and the properties and number of evacuation person. Using the obtained data, the passage prediction model is trained with a deep learning, and the passage time is predicted through the learned model. Construct a numerical map of a naval ship and construct a distance matrix of the vessel using predicted passage time data. The distance matrix configured in one of the path search algorithms, the Dijkstra algorithm, is applied to explore the evacuation path of naval ship.

• 한국정보전자통신기술학회논문지
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• 제15권1호
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• pp.45-55
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• 2022

현재 Collective Control 알고리즘은 가장 대중화되어있는 엘리베이터 알고리즘이다. Collective Control 알고리즘은 엘리베이터의 움직임 방향과 사용자의 도착지 방향이 같을 때 사용자가 엘리베이터를 이용할 수 있다. 하지만, 해당 알고리즘은 사용자의 엘리베이터의 대기시간과 여러 대의 엘리베이터를 사용할 경우, 주로 한 엘리베이터만 사용자들의 호출에 반응하는 문제점이 있다. 이러한 문제점을 해결하고자 본 논문에서는 HRN (Highest Response Ratio Next) 알고리즘에서 착안한 새로운 Hybrid HRN 알고리즘을 제안하고자 한다. 일반적으로 HRN 알고리즘은 사용자의 탑승 시간과 하차 시간이 필요하지만 엘리베이터 특성상 사용자의 호출이 언제 발생할지 예측이 어렵다. 따라서 이러한 한계를 극복하고자 본 논문에서는 사용자의 호출 위치와 도착 위치의 거리를 고려하는 Hybrid HRN 알고리즘을 제안한다. 본 논문에서는 실험을 통하여 제안한 Hybrid HRN 알고리즘이, 임의의 100명에 대한 사용자의 평균대기 시간은 23.34초, 표준 편차는 11.86, 엘리베이터의 총 움직인 거리는 535.2m, 총 운행 시간 84sec, 두 엘리베이터 사이의 운행 Balance는 92m의 성능을 나타냄으로써, 사용자 탑승대기 시간을 고려했을 때 기존에 제시된 Collective Control, Zoning, 3-Passage 알고리즘보다 보다 우수함을 보여준다.

• 한국의학물리학회지:의학물리
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• 제19권2호
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• pp.125-130
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• 2008

수의 임상에서 캡슐 내시경(capsule endoscopy, CE)의 사용에 기본적인 기준과 유용성을 정립하기 위하여 정상 중형견($7{\sim}13\;kg$)에서 캡슐의 위 통과시간(gastric transit time, GTT), 작은창자 통과시간(small intestinal transit time, SITT), 완전통과율(complete transit time, CTT)을 측정하고 통과율을 분석하였다. 1군(capsule 투여), 2군(capsule+water 투여), 3군(metoclopramide+capsule 투여) 그리고 (metoclopramide+capsule+water 투여)의 총 4군으로 나누어 방사선 촬영을 통하여 평가를 실시했다. GTT는 평균 $45{\sim}20\;min$ (group 1), $117{\pm}35\;min$ (group 2), $150{\pm}40\;min$ (group 3), $154{\pm}65\;min$ (group 4)였으며 SITT는 평균 $75{\pm}20\;min$ (group 1), $195{\pm}55\;min$ (group 2), $75{\pm}15\;min$(group 3), $76{\pm}15\;min$ (group 4)로 나타났다. 완전통과율(complete passage rate)은 각각 20% (group 1), 40%, (group 2), 20% (group 3), 50% (group 4)로 나타났다. 실험결과 캡슐이 유문부를 통과하기만 하면 내시경 캡슐 배터리 수명인 8시간 이내로 위장관을 모두 통과하였으며 물을 위내로 투여한 후 volume의 증가에 의한 위 운동성의 증가는 캡슐의 유문부 통과에 도움이 되었다. 그러나 metoclopramide의 투여는 유문부의 통과와 SITT의 시간에 대한 유의적인 효과가 나타나지 않았다.

• Coupled systems mechanics
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• 제7권4호
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• pp.421-440
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• 2018

In this study, a computational method for wall shear stress combined with an implicit direct-forcing immersed boundary method is presented. Near the immersed boundaries, the sub-grid stress is determined by a wall model in which the wall shear stress is directly calculated from the Lagrangian force on the immersed boundary. A coupling mathematical model of the transition process for a model Francis turbine comprising turbulent flow and rotating rigid guide vanes is established. The spatiotemporal distributions of pressure, velocity, vorticity and turbulent quantity are gained with the transient process; the drag and lift coefficients as well as other forces (moments) are also obtained as functions of the attack angle. At the same time, analysis is conducted of the characteristics of pressure pulsation, velocity stripes and vortex structure at some key parts of flowing passage. The coupling relations among the turbulent flow, the dynamical force (moment) response of blade and the rotating of guide vane are also obtained.

• 한국건축시공학회:학술대회논문집
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• 한국건축시공학회 2017년도 추계 학술논문 발표대회
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• pp.22-23
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• 2017

This research aimed to analyze the fundamental properties of cement mortar accompanying the change of solid contents of PC type water reducing agent. As a result of the experiment, in the case of flow to the properties of the fresh mortar, it shows a tendency to decrease as the solid content ratio decreases, and the decrease width with the passage of time is greatly reduced when 30 minutes passed since the passage of 60minutes It turned out that it was. In the case of the air contents and the compressive strength, it was found that there is almost no difference due to the change in the solid contents.

• Bulletin of the Korean Chemical Society
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• 제19권10호
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• pp.1068-1072
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• 1998

We present results of molecular dynamic (MD) simulations for the segmental motion of liquid n-butane as the base case for a consistent study for conformational transition from one rotational isomeric state to another in long chains of liquid n-alkanes. The behavior of the hazard plots for n-butane obtained from our MD simulations are compared with that for n-butane of Brownian dynamics study. The MD results for the conformational transition of n-butane by a Poisson process form the total first passage times are different from those from the separate t-g and g-t first passage times. This poor agreement is probably due to the failure of the detailed balance between the fractions of trans and gauche. The enhancement of the transitions t-g and g-t at short time regions are also discussed.

• 대한소아치과학회지
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• 제45권4호
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• pp.492-498
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• 2018

이 연구는 과잉치 치수로부터 얻은 세포를 더 이상 증식하지 못한다고 판단될 때까지 계대배양을 시행하면서 초기, 중기, 후기 계대의 특성을 비교 분석하였다. 2명의 건강한 6세 남아로부터 3개의 과잉치를 발거하여 치수조직에서 줄기세포를 얻었다. 이를 SNT1(supernumerary tooth 1), SNT2라고 하고, 다른 아이로부터 얻은 과잉치는 SNT3로 명명하였다. SNT1과 SNT2는 똑 같은 시간으로 계대배양 되었고 SNT3은 조금 빠르게 계대배양 되었다. 전체 계대배양의 평균 기간은 $3.6{\pm}1.1$일이었다. 총 $23.3{\pm}0.6$계대까지 배양되었으며, 83일이 소요되었다. 이를 계대를 기준으로 3등분하여 세 군으로 나누었다. I군에서 II군까지 계대배양에 소요된 시간 증가율은 11.90%였으나, II군에서 III군 사이의 증가율은 28.62%로 2.4배가 증가하였다. 22계대까지의 배양기간에 대한 소요 시간을 회귀분석한 결과 y = 0.1169x + 2.25 ($r^2=0.4778$)과 y = 0.1169x + 2.0 ($r^2=0.6444$)으로 추론되었다. 과잉치 치수유래 줄기세포의 정상 배양은 20계대까지 가능할 것으로 판단되었으며, 후기로 넘어가면 계대배양 시간이 2.4배 증가였다. 후기 계대에서는 세포내 부유물이 증가하였고, 형태도 선형으로 변형되는 양상이 관찰되었다.