• Asian Pacific Journal of Cancer Prevention
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• v.16 no.3
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• pp.941-945
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• 2015

Semaphoring is a transmembrane receptor which participates in many cytokine-mediated signal pathways that are closely related to the angiogenesis, occurrence and development of carcinoma. The present study was designed to access the effect of mono-antibody (mAb) guided radioimmunotherapy (RIT) on skin carcinoma and investigate the potential mechanisms. Semaphoring mAb was acquired from mice (Balb/c), purified with rProtein A column; purity, concentration and activity were tested with SDS-PAGE and indirect ELISA; specificity and expression on the cutanuem carcinoma line and tissue were tested by Western blotting; morphology change was assessed by microscopy. MTT assay and colony inhibition tests were carried out to test the influence on the proliferation of tumor cells; Western blotting was also carried out for expression of apoptosis-associated (caspase-3, Bax, Bcl-2) and proliferation-related (PI3K, p-Akt, Akt, p-ERK1/2, ERK1/2) proteins and analyse the change in signal pathways (PI3K/Akt and MEK/ERK). The purity of purified semaphorin mAb was 96.5% and the titer is about $1{\times}10^6$. Western blotting showed semaphoring mAb to have specifically binding stripes with semaphoring b1b2 protein, B16F10, and A431 cells at 39KDa, 100KDa and 130KDa, respectively. Positive expression was detected both in cutanuem carcinoma line and tissue and it mostly located in cell membranes. MMT assay revealed dose-relate and time-relate inhibitory effect of semaphorin mAb on A431 and B16F10. Colony inhibition tests also showed dose-relate inhibitory effects. Western blotting demonstrated the expression of apoptosis and proliferation-related protein and changes in signal pathway. In conclusion, we demonstrated that semaphorin is highly expressed on the tumor cell-surfaces and RIT with semaphorin mAb has effect in i nhibiting proliferation and accelerating apoptosis of tumor cells.

• Journal of Ginseng Research
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• v.44 no.5
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• pp.725-737
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• 2020

Background: T-cell acute lymphoblastic leukemia (T-ALL) is a kind of aggressive hematological cancer, and the PI3K/Akt/mTOR signaling pathway is activated in most patients with T-ALL and responsible for poor prognosis. 20(S)-Ginsenoside Rh2 (20(S)-GRh2) is a major active compound extracted from ginseng, which exhibits anti-cancer effects. However, the underlying anticancer mechanisms of 20(S)-GRh2 targeting the PI3K/Akt/mTOR pathway in T-ALL have not been explored. Methods: Cell growth and cell cycle were determined to investigate the effect of 20(S)-GRh2 on ALL cells. PI3K/Akt/mTOR pathway-related proteins were detected in 20(S)-GRh2-treated Jurkat cells by immunoblotting. Antitumor effect of 20(S)-GRh2 against T-ALL was investigated in xenograft mice. The mechanisms of 20(S)-GRh2 against T-ALL were examined by cell proliferation, apoptosis, and autophagy. Results: In the present study, the results showed that 20(S)-GRh2 decreased cell growth and arrested cell cycle at the G1 phase in ALL cells. 20(S)-GRh2 induced apoptosis through enhancing reactive oxygen species generation and upregulating apoptosis-related proteins. 20(S)-GRh2 significantly elevated the levels of pEGFP-LC3 and autophagy-related proteins in Jurkat cells. Furthermore, the PI3K/Akt/mTOR signaling pathway was effectively blocked by 20(S)-GRh2. 20(S)-GRh2 suppressed cell proliferation and promoted apoptosis and autophagy by suppressing the PI3K/Akt/mTOR pathway in Jurkat cells. Finally, 20(S)-GRh2 alleviated symptoms of leukemia and reduced the number of white blood cells and CD3 staining in the spleen of xenograft mice, indicating antitumor effects against T-ALL in vivo. Conclusion: These findings indicate that 20(S)-GRh2 exhibits beneficial effects against T-ALL through the PI3K/Akt/mTOR pathway and could be a natural product of novel target for T-ALL therapy.

• Journal of applied mathematics & informatics
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• v.27 no.1_2
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• pp.161-173
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• 2009

In this paper, we introduce an iterative scheme for finding a common element of the set of fixed points of a nonexpansive mapping, the set of solutions of the variational inequality for an inverse-strongly monotone mapping and the set of solutions of an equilibrium problem in a Hilbert space. We show that the iterative sequence converges strongly to a common element of the three sets. The results of this paper extend and improve the corresponding results announced by many others.

• International Journal of Quality Innovation
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• v.5 no.1
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• pp.43-51
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• 2004

During process design or process optimization, it is quite common for experimenters to find optimum operating conditions for several responses simultaneously. The traditional multiresponse surfaces optimization methods do not consider the uncertain relationship among these responses sufficiently. For this reason, the authors propose an optimization method based on evidential reasoning theory by Dempster and Shafer. By maximizing the basic probability assignment function, which indicates the degree of belief that certain operating condition is the solution of this multiresponse surfaces optimization problem, the desirable operating condition can be found.

• International Journal of Quality Innovation
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• v.9 no.2
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• pp.31-38
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• 2008

Product liability refers to the liability of producers, distributors and suppliers for consumers' property losses or personal injury caused by that product. This paper conducted a survey related to product liability issues in Shenzhen City, and did an in-depth analysis of current problems. Finally, the paper puts forward corresponding suggestions for improvement of Shenzhen City product liability status.

• Proceedings of the Korean Society of Coastal and Ocean Engineers Conference
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• 1996.10a
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• pp.42-44
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• 1996

Recently due to repeatedly occurrence of flood, a lot of Chinese cities accept new design criteria for their protective project Most of them calculated by a certain type of probability distribution. In order to meet the demand of development economy the return period of design criteria is changed more longer and longer even 1000years, but the data which the calculation dependent on is only about 30-40 years. (omitted)

• Bulletin of the Korean Mathematical Society
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• v.55 no.4
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• pp.1137-1147
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• 2018

In this paper, we discovered a sufficient condition ensuring the weighted composition operators $C_{{\omega}_1,{\varphi}_1},{\cdots},C_{{\omega}_N,{\varphi}_N}$ were disjoint supercyclic on $H({\Omega})$ endowed with the compact open topology. Besides, we provided a condition on inducing symbols to guarantee the disjoint supercyclicity of non-constant adjoint multipliers $M^*_{{\varphi}_1},M^*_{{\varphi}_2},{\cdots},M^*_{{\varphi}_N}$ on a Hilbert space ${\mathcal{H}}$.

• Communications of the Korean Mathematical Society
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• v.27 no.3
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• pp.505-512
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• 2012

In this paper, we introduce an iterative scheme for finding a common element of the set of fixed points of a nonexpansive mappings and nonspreading mappings and the set of solution of an equilibrium problem on the setting of real Hilbert spaces.

• Bulletin of the Korean Mathematical Society
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• v.52 no.3
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• pp.987-998
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• 2015

In the paper, the authors discover an integral representation, some inequalities, and complete monotonicity of the Bernoulli numbers of the second kind.

• Bulletin of the Korean Mathematical Society
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• v.52 no.3
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• pp.751-759
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• 2015

In this paper, we give new characterizations of the boundedness and compactness of composition operators $C_{\varphi}$ between Bloch type spaces in the unit ball $\mathbb{B}^n$, in terms of the power of the components of ${\varphi}$, where ${\varphi}$ is a holomorphic self-map of $\mathbb{B}^n$.