• Asian-Australasian Journal of Animal Sciences
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• 제13권12호
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• pp.1731-1737
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• 2000

This study was conducted to investigate the effects of threonine:lysine ratios on growth performance, apparent nutrient digestibility and blood urea nitrogen (BUN) concentration, and to estimate the optimal threonine:lysine ratios for growing barrows and gilts. A total of 150 pigs (Landrace${\times}$Yorkshire${\times}$Duroc, $16.75{\pm}0.42kg$ average body weight, 75 barrows and 75 gilts) was randomly allotted into six treatments in a $2{\times}3$ factorial design. Six diets were formulated to contain 1.12% lysine for barrows and 1.33% lysine for gilts with three threonine:lysine ratios (50, 60 and 70%) for both barrows and gilts. Throughout the whole experimental period (16 to 56 kg body weight), there was no interaction between sex and dietary threonine:lysine ratio in average daily gain (ADG), average daily feed intake (ADFI) and feed conversion rate (FCR). Between sexes, there was a clear sex-effect showing better growth performance of barrows. Barrows consumed more feed (p<0.01) and grew faster (p<0.01) than gilts. For barrows, there was a trend to improved ADG and FCR with increasing threonine:lysine ratio. For gilts, there was a trend to improved ADG and FCR up to threonine:lysine ratio of 60%, but not significant. There was no interaction between sex and threonine:lysine ratio in nutrient digestibilities of growing pigs except for crude ash (CA). Between sexes, there were differences in nutrient digestibilities, except for calcium for which gilts showed higher a digestibility (p<0.01). Among dietary threonine:lysine ratios, there were no differences in nutrient digestibilities. Mean values of essential amino acids (EAA), non-essential amino acids (NEAA) and total amino acids (TAA) digestibilities were not affected by sex and dietary threonine:lysine ratio. There was no evidence of an interaction between sexes and dietary threonine:lysine ratio. Between sexes, total BUN concentration was lower in gilts than barrows (p<0.05). It was concluded that a 70 and 60% dietary threonine:lysine ratio for barrows (1.12% lysine) and gilts (1.33% lysine) tended to result in better growth performances and nutrient utilization and lower BUN concentration than other threonine:lysine ratios.

• Asian-Australasian Journal of Animal Sciences
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• 제11권1호
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• pp.43-50
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• 1998

Three hundred and fifty-two growing pigs were utilized in three growth trials to investigate the effects of crystalline amino acid supplementation on growing pigs' growth performance and feed consumption. In Exp. 1, diets were formulated on the basis of digestible amino acids. Utilization of rapeseed meal plus cottonseed meal (R + C) in diet supported similar average daily gain (ADG), feed/gain (F/G) and cost per gain (p > 0.05) to group of soybean meal (SBM). In Exp. 2, 14% CP diets containing equal amount of cottonseed meal and rapeseed meal were formulated with the addition of threonine and/or tryptophan. Supplementation with threonine improved ADG and F/G (p < 0.05). Supplementation with only tryptophan made no benefits for ADG of growing pigs. In Exp. 3, diets with different threonine level were formulated. Increasing dietary threonine to 0.54% improved (p < 0.05) ADG and F/G compared to 0.45% dietary threonine. Increase in dietary threonine did not improve ADG (p > 0.05) but decreased F/G (p < 0.05) because of a decrease in average daily feed intake. Pigs fed diet with dietary threonine/lysine ratio of 67.5% supported optimum growth performance.

• Animal Bioscience
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• 제34권12호
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• pp.1987-1994
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• 2021

Objective: The present study aimed to evaluate the effects of varying standardized ileal digestible lysine:threonine (SID Lys:Thr) ratio in the diet on the performance and meat quality of finishing pigs. Methods: In total 192 crossbred pigs ([Landrace×Yorkshire]×Duroc, 17 weeks old), with an initial body weight (BW) of 70.6±3.9 kg were used in an 8-wk trial. Pigs were randomly allotted to one of six dietary treatments based on their initial BW and sex (8 replications; 4 pigs per pen, 2 barrows and 2 gilts). The pigs in the 6 treatments were fed diets having different SID Lys:Thr ratios such as 1:0.65, 1:0.66, 1:0.67, 1:0.68, 1:0.69, and 1:0.70. Results: A linear increment (p<0.05) in average daily gain (ADG) and trends in reduction in feed conversion ratio (FCR) were observed during day 29 to 56 of the experiment and the apparent total tract digestibility (ATTD) of dry matter tended to increase linearly (p = 0.094) at the end of the experiment (day 56) with the increase in the dietary SID Lys:Thr ratios. The backfat thickness and lean percentage increased (linear effect, p<0.05) on day 28. In addition, at day 56, a linear (p<0.05) increment in lean percentage was observed. Significant quadratic responses (p = 0.02) for pH and drip loss at day 7 (p = 0.02), a linear increase (p<0.05) in cooking loss and drip loss at day 7, and a trend in quadratic response (p = 0.07) in the lightness of meat color (L^*) were observed, whereas other meat quality indices were unaffected by varying the SID Lys:Thr ratios. Conclusion: The SID Lys:Thr ratio for maximum ADG, minimum FCR and enhanced digestibility was found to be 0.70. However, for carcass trait and meat quality, the SID Lys:Thr ratio of 0.65 was enough.

• Asian-Australasian Journal of Animal Sciences
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• 제11권1호
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• pp.21-29
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• 1998

Four feeding trials with 260 pigs were conducted to evaluate the effects of supplementing the diet with different amino acids on growth performance and blood metabolites for weanling, growing and finishing pigs. One hundred twenty weanling pigs (Exp. 1, BW 8 kg), eighty growing pigs (Exp. 2. BW 20 kg), thirty growing pigs (Exp. 3, BW 29 kg) and thirty finishing pigs (Exp. 4, BW 50 kg) were randomly allotted to different dietary treatments according to sex and body weight. Pigs weight and feed consumption were measured at initiation and termination of each trial with 4 weeks. At the end of trial, blood samples from three pigs selected in each pen (Exp. 1) and each pig (Exp. 2) were obtained to determine the level of blood urea nitrogen, glucose, insulin and cortisol in the serum. In Exp. 1, pigs fed diet supplemented both with lysine and methionine had the best feed conversion ratio (p < 0.05), but no significant differences (p > 0.05) were observed in ADG and ADFI. Pigs receiving control diet obtained the obtained the optimal ADG (p < 0.05), ADFI (p < 0.05) and F/G for the whole period. No differences were detected in serum glucose, insulin and cortisol concentrations. In Exp. 2, pigs receiving the control diet exhibited the lowest serum urea nitrogen (p < 0.05), ADG, F/G and serum insulin concentration increased linearly (p < 0.05) with the inclusion of lysine, methionine, threonine and tryptophan in diets. No significant differences (p > 0.05) were detected for glucose and cortisol content in pigs serum among dietary treatments. In Exp. 3 and 4, pigs growth rate increased linearly (p < 0.01), and feed conversion efficiency was also improves by addition of lysine, methionine, threonine and tryptophan. In conclusion, pigs fed diets supplemented with lysine, methionine, threonine and tryptophan together obtained optimal growth performance in growing and finishing periods.

• Asian-Australasian Journal of Animal Sciences
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• 제26권8호
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• pp.1172-1180
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• 2013

Two experiments were conducted to determine the standardized ileal digestible (SID) lysine (Lys) requirement and the ideal SID threonine (Thr) to Lys ratio for finishing barrows. In Exp. 1, 120 barrows with an average body weight of $72.8{\pm}3.6$ kg were allotted to one of six dietary treatments in a randomized complete block design conducted for 35 d. Each diet was fed to five pens of pigs containing four barrows. A normal crude protein (CP) diet providing 15.3% CP and 0.71% SID Lys and five low CP diets providing 12% CP with SID Lys concentrations of 0.51, 0.61, 0.71, 0.81 and 0.91% were formulated. Increasing the SID Lys content of the diet resulted in an increase in weight gain (linear effect p = 0.04 and quadratic effect p = 0.08) and an improvement in feed conversion ratio (FCR) (linear effect p = 0.02 and quadratic effect p = 0.02). For weight gain and FCR, the estimated SID Lys requirement of finishing barrows were 0.71 and 0.71% (linear broken-line analysis), 0.79 and 0.78% (quadratic analysis), respectively. Exp. 2 was a 26 d dose-response study using SID Thr to Lys ratios of 0.56, 0.61, 0.67, 0.72 and 0.77. A total of 138 barrows weighing $72.5{\pm}4.4$ kg were randomly allotted to receive one of the five diets. All diets were formulated to contain 0.61% SID Lys (10.5% CP), which is slightly lower than the pig's requirement. Weight gain was quadratically (p = 0.03) affected by SID Thr to Lys ratio while FCR was linearly improved (p = 0.02). The SID Thr to Lys ratios for maximal weight gain and minimal FCR and serum urea nitrogen (SUN) were 0.67, 0.71 and 0.64 using a linear broken-line model and 0.68, 0.78 and 0.70 using a quadratic model, respectively. Based on the estimates obtained from the broken-line and quadratic analysis, we concluded that the dietary SID Lys requirement for both maximum weight gain and minimum FCR was 0.75%, and an optimum SID Thr to Lys ratio was 0.68 to maximize weight gain, 0.75 to optimize FCR and 0.67 to minimize SUN for finishing barrows.

• Applied Biological Chemistry
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• 제50권2호
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• pp.116-120
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• 2007

주요 수산가공자원의 하나인 오징어의 소비 촉진을 위하여 오징어포의 제조를 시도하였다. 오징어의 효율적 이용 측면에서 조미 오징어포의 제조는 오징어를 70% 이상으로 첨가하는 것이 적절하리라 판단되었다. 오징어와 수리미를 70:30의 비율을 사용하여 제조한 조미 오징어포는 조미 수리미포에 비하여 총 아미노산의 함량이 높았으며, 주요 아미노산으로는 glutamic acid, leucine, lysine 및 threonine 등이었다. 또한, 조미 오징어포의 칼슘 및 인 함량은 조미 수리미포에 비해 높았으며, 조미 오징어포의 유리아미노산의 총함량 및 taset value는 각각 3,199.1 mg/100g 및 402.00이었고 맛에 지대하게 영향을 미치는 아미노산은 glutamic acid 및 aspartic acid이었다.

• Asian-Australasian Journal of Animal Sciences
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• 제24권11호
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• pp.1623-1628
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• 2011

The effects of dietary lysine (Lys) at a fixed ratio to crude protein (CP) and essential amino acids (EAA) on early growth response of broilers were studied. Four diets were formulated to contain similar metabolizable energy (ME, 2,950 kcal/kg) but contained graded levels of incremental Lys (1.1, 1.2, 1.3 and 1.4%) while also increasing the dietary CP and EAA (methionine, methionine+ cystine, threonine and tryptophan) to maintain a constant ratio with Lys. Each diet was fed at random to 10 replicates of 6 chicks each throughout the experimental period (1-21 d). At the lowest concentration of Lys of 1.1% (19.04% CP), body weight gain (BWG) was lowest and feed conversion ratio (FCR) was poorest. The BWG increased and FCR decreased linearly as dietary Lys increased upto 1.3% (22.5% CP). Lowest feed consumption was observed in the dietary group that contained 1.1% Lys (19.04% CP) in the diet. Increasing the concentration of Lys to 1.2% (20.77% CP), significantly increased the feed consumption. The concentrations of protein, calcium, phosphorus and cholesterol in serum were not influenced by the variation in Lys contents in the diet. The humoral immune response as measured by antibody titre in response to SRBC inoculation was significantly lower in the diets containing 1.1% Lys compared to 1.4%. It is concluded that the Lys requirement of broilers is 1.3% (22.5% CP) during 0 to 21 days of age for eliciting optimum performance when a fixed ratio of Lys to CP (1:17.31) and essential AA is maintained (1:0.47 Met; 1:0.56 Thr; 1:0.17 Try).

• Asian-Australasian Journal of Animal Sciences
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• 제18권8호
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• pp.1164-1170
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• 2005

This study was conducted to evaluate performance, breast (thigh) muscle yield, nutrient digestibility and serum biochemical parameters of broilers fed low-protein diets supplemented with various threonine to lysine ratios (Thr/Lys). Three hundred and twenty four day-old male Arbor Acres broilers were randomly allotted to six dietary treatments with six replicates per treatment and nine broilers per replicate. Six treatments included one control diet [formulated according to NRC (1994)], and five experimental diets (treatments 1-5). In treatments 1-5, the crude protein level was 2% lower than that of the NRC control diet. The Thr/Lys in treatments 1-5 was 0.65, 0.70, 0.75, 0.80 and 0.85 during the starter and grower phases and 0.70, 0.75, 0.80, 0.85 and 0.90 during the finisher phase with other nutrient levels kept consistent. The results showed that: (1) With increasing Thr/Lys, ADG of treatments 1, 3 and 4 were lower (p<0.05) than those of the NRC control diet during the starter phase; ADG of treatments 1-5 were lower (p<0.05) than those of the NRC control during the grower phase, and ADG of treatment 1 was lower (p<0.05) than that of the NRC control during the finisher phase. Average daily feed intake in treatments 1 and 4 were lower (p<0.05) than that of broilers on the NRC control diet during the grower phase. Feed/gain (F/G) of the NRC control diet was lowest (p<0.05) during the starter phase. F/G in treatments 2 and 5 were higher (p<0.05) than that of NRC during the grower phase. (2) Breast muscle proportion on d 21 increased linearly (p<0.05) in response to graded levels of Thr/Lys. (3) No differences were detected for dry matter, energy and crude protein digestibility among all seven treatments. (4) On d 21, serum triglyceride of broilers on dietary treatments 3 and 5 was higher (p<0.05) than that of broilers in control. The results indicate that the low-protein diets supplemented with an appropriate Thr/Lys could not support the same performance that was achieved by the broilers fed the NRC control diet.

• Asian-Australasian Journal of Animal Sciences
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• 제25권2호
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• pp.224-233
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• 2012

The limiting sequence and relative ratio of lysine (Lys), methionine (Met), and threonine (Thr) for calves about 2 mo of age fed milk replacers (MR) containing soy protein are not clearly defined. The objective of the study was to investigate the effect of supplementing MR containing 22% CP, half from soy protein concentrate (SPC, 40.56% CP, flour) and half from whey proteins, with Lys, Met, and Thr to estimate amino acid (AA) sequence and their relative ratio for calves about 2 mo of age. A method of partial deduction of AA was adopted. Twenty-four newborn calves (half males and half females, $40.7{\pm}0.9$ kg of BW) were fed 1 of 4 MR diets for 56 d (n = 6/diet). The diets were supplemented with all (positive control) or with 2 of the 3 AAs: Lys, Met and Thr, (i.e., PC (22% CP, 2.34% Lys, 0.72% Met and 1.80% Thr), PC-Lys (22% CP, 1.64% Lys, 0.72% Met and 1.80% Thr), PC-Met (22% CP, 2.34% Lys, 0.50% Met and 1.80% Thr), and PC-Thr (22% CP, 2.34% Lys, 0.72% Met and 1.26% Thr)). Calves were fed thrice daily; starter (20% CP, 1.03% Lys, 0.30% Met and 0.69% Thr), hay (3.23% CP, 0.29% Lys, 0.12% Met and 0.23% Thr) and water were offered free choice. Starter and hay were only offered beginning on d 36 (after 5 wk) and d 43 (after 6 wk), respectively. BW, body size and blood samples measures were taken every two weeks. Three-day total collection of feed refusals, feces, and urine were recorded starting at d 33 and d 54 of age, respectively. From the results, the limiting sequence and relative ratio between the 3 AAs in calves with different diet structures were calculated. The limiting sequence of the 3 AAs were ranked as Lys, Met and Thr; the proper ratio was 100:29:70 for MR-only diet and 100:30:60 for diets consisted of MR, starter and hay. Nitrogen digestion and utilization and nutrient digestibility were negatively affected by AA deletion treatments. From the evidence of this experiment, it did not appear that the AA limiting sequence was selectively altered by differences in diet structures such as would be encountered in practice. The relative ratio between the 3 AAs varied with the offer of starter and hay to calves, and the average ratio was 100:29.5:65 for calves during 2 to 10 wk of age.

• 한국수산과학회지
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• 제12권3호
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• pp.191-200
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• 1979

새로운 단백질자원인 남빙양산크릴의 효율적인 이용을 위한 기초자료를 얻기 위하여 식품원료학적인 특성의 구명에 주목적을 두어 연구를 수행하였다. 즉 1978년 1월 30일 남비양에서 어획하여 생동결 및 자숙동결한 크릴을 시료로 하여 일반성분조성, 아미노산조성, 중금속류의 함량, 가식부의 비율, 해동시의 drip 발생량 및 그 결성 등에 대하여 연구검토하였으며, 그 결과를 요약하면 다음과 같다. 1. 1. 일반성분조성에 있어서 생동결크릴은 자숙동결크릴에 비하여 지방 및 유리아미노실소의 함량이 높고 회분함량이 낮았다. VBN량은 생동결크릴이 $37.6mg\%$, 자숙동결크릴이 $26.4mg\%$로 상당히 높고, pH는 생동결크릴은 7.1, 자숙동결크릴이 7.2로 비교적 낮았는데, 이러한 VBN 및 pH값은 시료 크릴의 저장기간이 길고, 냉동보관의 빈번한 변동으로 선도가 많이 저하한 때문이라고 생각된다. 생동결크릴의 육질부아미노산조성은 glutamic acid, aspartic acid, lysine, proline 및 leucine의 함량이 높고, methionine, histidine, serine, tyrosine 및 phenylalanine의 함량이 적었으며, 필수아미노산에 있어서는 lysine 및 leucine의 함량이 높고 methionine 및 phenylalanine의 함량이 낮았다. 한편 유리아미노산조성에 있어서는 proline, lysine, glycine, arginine 및 alanine의 함량이 높고 histidine, methionine, aspartic acid, serine 및 threonine의 함량이 낮았으며, 필수아미노산에 있어서는 lysine 및 leucine의 함량이 많고 methionine 및 threonine의 함량이 낮았다. 크릴의 구성아미노산이나 유리마미노산 조성에 있어서 필수아미노산인 lysine의 함량이 많은 점은 영양적으로 의의가 크다고 할 수 있다. 3. 크릴의 중금속함량은 Hg가 $0.039\~0.048ppm$, Pb가 $0.06\~0.11ppm$, Zn가 0.32이하 Cd가 $0.008\~0.012ppm$, C가 ND, Fe가 $0.61\~0.68ppm$, Cu가 $0.87\~1.37ppm$로 전반적으로 함량이 낮았다. 단 Cu의 함량이 비교적 높은 것은 갑각류의 혈액구성성분인 때문이라고 생각된다. 4. 가식부와 부하식부의 비율은 생동결크릴이 37:63이고 자숙동결크릴이 42:58로 외견상 가식부비율이 자숙동결크릴이 많았다. 5. 해동시의 drip의 유출량은 생동결크릴이 약 $36\%$ 자숙동결이 약 $24\%$로 생동결크릴이 많았고, 크릴의 성분조성에 있어서는 생동결크릴의 경우가 고형조단백질 및 조지방의 함량이 현저하게 많았고 회분함량은 적었다.