• BMB Reports
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• v.40 no.4
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• pp.571-576
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• 2007

Three anti-apoptosis genes, Ls-iap2, iap3 and p49 were found in Leucania separata multiple nuclear polyhedrovirus. Amino acid sequence homology of Ls-IAP2 and Ls-IAP3 with Op-IAP2 and Op-IAP3 from Orgyia pseddotsugata MNPV were 20% and 42%, while that of Ls-P49 is 28% with Sl-P49 from Spodoptera littorolis MNPV. Ls-IAP2 contains one baculoviral IAP repeat (BIR) domain followed by a RING domain, while Ls-IAP3 contains two BIRs and a RING. Ls-P49 contains a reactive site loop, predicted cleavage site (KKLD$^{74}{\downarrow}$G) that is different from Sl-P49 (TVID$^{94}{\downarrow}$G). Expressed Ls-iap3 or Ls-p49 under presence of actinomycin D in SF9 cells, DNA ladder assayrevealed that Ls- IAP3 or Ls-P49 could block the apoptosis of SF9 cells induced by actinomycin D. Replication of p35 deficient-mutant Autographa californica MNPV in SF9 cells was also rescued when Ls-iap3 or Ls-p49 was expressed transiently. No anti-apoptotic activity was observed for Ls-IAP2. The results showed that both of Ls-IAP3 and Ls-P49 were functional apoptotic suppressors in SF9 cells.

• The Journal of Korean Institute of Electromagnetic Engineering and Science
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• v.18 no.11
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• pp.1262-1270
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• 2007

In this paper, we propose a new distortion cancellation mechanism for a balanced power amplifier structure using the carrier cancellation loop of a feedforward and post-distortion technique. The proposed cross post-distortion balanced linear amplifier can reduce nonlinear components as much as the conventional feedforward amplifier through the output dynamic range and broad bandwidth. Also the proposed system provides higher efficiency than the feedforward. The capacities of power amplifier and error power amplifier in the proposed system are analyzed and compared with those of feedforward amplifier. Also the operation mechanisms of the three kind loops are explained. The proposed cross post-distortion balanced linear power amplifier is implemented at the IMT-2000($f_0=2.14\;GHz$) band. With the commercial high power amplifiers of total power of 240 W peak envelope power fer base-station application, the adjacent channel leakage ratio measurement with wideband code division multiple access 4FA signal shows 18.6 dB improvement at an average output power of 40 dBm. The efficiency of fabricated amplifier Improves about 2 % than the conventional feedforward amplifier.

• Korean Journal of Cognitive Science
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• v.22 no.2
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• pp.193-215
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• 2011

The purpose of this study was to examine the working memory that is related with reading. For this purpose, the third and fourth grade children with reading difficulty were compared with normal children. The gender comparison and the correlations among the three components of working memory were also examined. The results indicated that the reading difficulty group were outperformed by the normal group in every test. Gender difference was found only in visuospatial capacity. There was an interaction between reading and gender in the memory of dynamic mazes. In the whole group, every correlation among all components was significant. When the correlations were examined separately in each group, however, only one correlation was significant in both groups. When the influence of the central executive was controlled, the interrelationship between phonological working memory and visuospatial working memory was mitigated. This study suggests that when teaching children with reading difficulty instructional material should be succinct and visuospatial information needs to be used for boys if possible.

• Journal of the Korean Society for Railway
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• v.13 no.2
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• pp.152-158
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• 2010

Generally, the AC electrified railway systems have the power quality problems that are induced from the harmonic currents and the reactive power. This paper presents a single-phase hybrid active filter adopting a SRF(synchronous-reference-frame) control for improving power quality in the AC electrified railway systems. The single-phase hybrid active filter can compensate the harmonic currents and the reactive power through the proposed SRF control algorithm. The proposed control algorithm can extract the third and fifth harmonics through the MSRF(multiple-synchronous-reference-frames) which is used to apply the three-phase systems. Therefore, the hybrid active filter can compensates only the high-frequency harmonic currents whereas the passive filter compensates the low-frequency harmonic currents. Also, the proposed SRF control algorithm can compensate the reactive power by the closed-loop control. The Validity and the effectiveness of the proposed SRF control method for the hybrid active filter are illustrated through the simulation results.

• Journal of the Korean Institute of Illuminating and Electrical Installation Engineers
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• v.12 no.3
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• pp.14-21
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• 1998

In this paper, the measurements of ELF electric and magnetic fields due to double circuit 345[kV} transmission lines are made using planar-type electric field sensor and multitum loop-type magnetic field sensor, and the magnitudes of electric and magnetic fields are illustrated by a three-dimensional plot. Also, in order to predict the magnetic field strength with lad variation, a typical daily load current curves of the transmission lines are displayed because the magnetic field is changed with load current. experimental results of ELF electric and magnetic fields along center line versus lateral distance are compared with the theoretical values computed by using the FIELDS program. The electric field intensity in and around a transmission tower is lowered, and the greatest point of the magnetic field is shifted to the heavy load line but generally is given the trend that the peak value appear at the central part of the transmission tower. The magnitudes of the maximum electric and magnetic fields in the vicinity of a transmission tower are less than 3.5[kV/m] and $20[{\mu}T]$, respectively. The measured electric and magnetic fields are satisfied with limits and guidelines recommended by various authorized international institutes.

• Plant Biotechnology Reports
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• v.4 no.3
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• pp.201-211
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• 2010

We present a highly effective T-DNA inserted gene screening method as part of a reverse genetics model system using the Chinese cabbage (Brassica rapa L. spp. pekinensis). Three-step two-dimensional (2D) matrix strategies are potentially accurate and useful for the identification of specific T-DNA inserted mutants from a large population. To construct our Chinese cabbage model, we utilized a forward genetics screening approach for the abnormal phenotypes that were obtained from transgenic plants of Brassica rapa generated with Agrobacteria tumefaciens containing the pRCV2 vector. From one transgenic plant with an abnormal phenotype, we observed that the st1 gene (which is related to senescence-associated process proteins) contained a T-DNA fragment, and that its expression level was decreased. This T-DNA insert was then used as a control to construct an effective screening pool. As a result, the optimum template concentration was found to be 0.1-1 ng in our PCR strategy. For other conditions, positive changes to the Gibbs free energy prevented the formation of oligo dimers and hairpin loop structures, and autosegment extension gave better results for long fragment amplification. Using this effective reverse genetics screening method, only 23 PCR reactions were necessary to select a target gene from a pool of 100 individual DNAs. Finally, we also confirmed that the sequence we obtained from the above method was identical to the flanking sequence isolated by rescue cloning.

• Journal of Korean Society of Transportation
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• v.26 no.2
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• pp.157-166
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• 2008

This study proposed a model to estimate traffic accident likelihood using real-time traffic data obtained from freeway traffic surveillance systems. Traffic variables representing spatio-temporal variations of traffic conditions were utilized as independent variables in the proposed models. Binary logistics regression modelings were conducted to correlate traffic variables and accident data that were collected from the Seohaean freeway during recent three years, from 2004 to 2006. To apply more reliable traffic variables, outlier filtering and data imputation were also performed. The outcomes of the model that are actually probabilistic measures of accident occurrence would be effectively utilized not only in designing warning information systems but also in evaluating the effectiveness of various traffic operations strategies in terms of traffic safety.

• Proceedings of the Korean Vacuum Society Conference
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• 2013.02a
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• pp.498-498
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• 2013

Atmospheric Pressure Plasmas have pioneered a new field of plasma for biomedical application bridging plasma physics and biology. Biological and medical applications of plasmas have attracted considerable attention due to promising applications in medicine such as electro-surgery, dentistry, skin care and sterilization of heat-sensitive medical instruments [1]. Traditional approaches using electronic devices have limits in heating, high voltage shock, and high current shock for patients. It is a great demand for plasma medical industrial acceptance that the plasma generation device should be compact, inexpensive, and safe for patients. Microwave-excited micro-plasma has the highest feasibility compared with other types of plasma sources since it has the advantages of low power, low voltage, safety from high-voltage shock, electromagnetic compatibility, and long lifetime due to the low energy of striking ions [2]. Recent experiment [2] shows three-log reduction within 180-s treatment of S. mutans with a low-power palm-size microwave power module for biomedical application. Experiments using microwave plasma are discussed. This low-power palm-size microwave power module board includes a power amplifier (PA) chip, a phase locked loop (PLL) chip, and an impedance matching network. As it has been a success, more compact-size module is needed for the portability of microwave devices and for the various medical applications of microwave plasma source. For the plasma generator, a 1.35-GHz coaxial transmission line resonator (CTLR) [3] is used. The way of reducing the size and enhancing the performances of the module is examined.

• Journal of Microbiology and Biotechnology
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• v.25 no.11
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• pp.1827-1834
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• 2015

The SMG1 lipase from Malassezia globosa is a newly found mono- and diacylglycerol (DAG) lipase that has a unique lid in the loop conformation that differs from the common alpha-helix lid. In the present study, we characterized the contribution of three residues, L103 and F104 in the lid and F278 in the rim of the binding site groove, on the function of SMG1 lipase. Site-directed mutagenesis was conducted at these sites, and each of the mutants was expressed in the yeast Pichia pastoris, purified, and characterized for their activity toward DAG and p-nitrophenol (pNP) ester. Compared with wild-type SMG1, F278A retained approximately 78% of its activity toward DAG, but only 11% activity toward pNP octanoate (pNP-C8). L103G increased its activity on pNP-C8 by approximately 2-fold, whereas F104G showed an approximate 40% decrease in pNP-C8 activity, and they both showed decreased activity on the DAG emulsion. The deletion of 103-104 retained approximately 30% of its activity toward the DAG emulsion, with an almost complete loss of pNP-C8 activity. The deletion of 103-104 showed a weaker penetration ability to a soybean phosphocholine monolayer than wild-type SMG1. Based on the modulation of the specificity and activity observed, a pNP-C8 binding model for the ester (pNP-C8, N102, and F278 form a flexible bridge) and a specific lipid-anchoring mechanism for DAG (L103 and F104 serve as "anchors" to the lipid interface) were proposed.

• Journal of Biomedical Engineering Research
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• v.29 no.4
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• pp.323-328
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• 2008

A patient with respiratory disorders such as a sleep apnea is increasing as the obese patient increase on the modern society. Positive Airway Pressure (PAP) devices are used in curing patient with respiratory disorders and turn out to be efficacious for patients of 75%. However, these devices are required for evaluating their performance to improve their performance by the mechanical breathing simulator. Recently, the mechanical breathing simulator was studied by the real time feedback control. However, the mechanical breathing simulator by an open loop control was specially required in order to analyze the effect of flow rate and pressure after operating the breathing auxiliary devices. Therefore the aims of this study were to make the mechanical breathing simulator by a piston motion and a valve function from the characteristic test of valve and motor, and to duplicate the flow rate and pressure profiles of some breathing patterns: normal and three disorder patterns. The mechanical simulator is composed cylinder, valve, ball screw and the motor. Also, the characteristic test of the motor and the valve were accomplished in order to define the relationship between the characteristics of simulator and the breathing profiles. Then, the flow rate and pressure profile of human breathing patterns were duplicated by the control of motor and valve. The result showed that the simulator reasonably duplicated the characteristics of human patterns: normal, obstructive sleep apnea (OSA), mild hypopnea with snore and mouth expiration patterns. However, we need to improve this simulator in detail and to validate this method for other patterns.