• The Korean Journal of Physiology and Pharmacology
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• v.10 no.4
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• pp.193-198
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• 2006

Many gastrointestinal muscles show electrical oscillation, so-called 'slow wave', originated from interstitial cells of Cajal (ICCs). Thus, a technique to freshly isolate the cells is indispensable to explore the electrophysiological properties of the ICCs. To apply an enzyme solution on the serosal surface for cell isolation, the intestine was inverted and 0.02% trypsin solution and 0.04% collagenase solution were applied to serosal cavity. After the enzyme treatment, mucosal layer was removed and longitudinal muscle layer was gently separated from the rest of tissue. The thin layer was stretched in the recording chamber and mounted on an inverted microscope. Using ${\beta}-escine$, perforated whole cell patch clamp technique was used. Under a microscope, the tissue showed smooth muscle cells and interstitial cells around the myenteric plexus. Under voltage clamp condition, three types of membrane potential were recorded. One group of interstitial cells, which were positive to methylene blue and CD34, showed spontaneous outward current. These cells had bipolar shape and were considered as fibroblast-like cells because of their peculiar shape and arrangement. Another group, positive to c-kit and methylene blue, showed spontaneous inward current. These cells had more rounded shape and processes and were considered as ICCs. The third, positive to c-kit and had granules containing methylene blue, showed quiet membrane potentials under the voltage-clamp mode. These cells appeared to be resident macrophages. Therefore, in the freshly isolated thin tissue preparation, methylene blue could easily identify three types of cells rather than morphological properties. Using this method, we were able to study electrical properties of fibroblast and residential macrophage as well as myenteric ICCs.

• Korean Journal of Heritage: History & Science
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• v.55 no.4
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• pp.38-53
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• 2022

In this article, I have summarized and reviewed the concepts and terms surrounding the stamped construction technique, focusing on earthen walls in the Three Kingdoms period. This is because confusion is caused by defining the nature of the earthen walls by using various concepts and terms for each researcher regarding the substance and construction method of the earthen walls. The stamped earth method is a representative ancient civil engineering or construction technique in which a frame is made of plates to form a fortress wall, a fence, and the base of a building, and then soil or sand is poured into it layer by layer and then stamped with a bat to make it solid. Therefore, in order to prove that the earthen wall was built by the stamped earth method, evidence such as a narrow plate, a column for fixing it, long horizontal and vertical wood pieces to support the narrow plate, and traces of pounding the soil must be detected. However, in Korea, there are very few cases where such evidence has been fully excavated, so it is necessary to agree on how strictly the standards for the stamped earth method will be applied. The terms related to the stamped technique mobilized for the construction of the earthen walls were explained with actual examples by dividing the terms related to the concept into terms related to the principle and unit of the stamped plate, and the specific stamped technology. In particular, in Pungnabtoseong Earthen wall, a variety of typical and diverse methods of building the ancient stamped earthen wall were identified so that decisive data could be secured to understand the principles and techniques of the stamped earthen wall. In the future, a more general understanding of the stamped technique will be possible only when more evidence related to it is found in relics other than Pungnabtoseong Earthen wall.

• Journal of Sensor Science and Technology
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• v.29 no.5
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• pp.354-359
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• 2020

The high vacuum hermetic sealing technique ensures excellent performance of MEMS resonators. For the high vacuum hermetic sealing, the customization of anodic bonding equipment was conducted for the glass/Si/glass triple-stack anodic bonding process. Figure 1 presents the schematic of the MEMS resonator with triple-stack high-vacuum anodic bonding. The anodic bonding process for vacuum sealing was performed with the chamber pressure lower than 5 × 10^-6 mbar, the piston pressure of 5 kN, and the applied voltage was 1 kV. The process temperature during anodic bonding was 400 ℃. To maintain the vacuum condition of the glass cavity, a getter material, such as a titanium thin film, was deposited. The getter materials was active at the 400 ℃ during the anodic bonding process. To read out the electrical signals from the Si resonator, a vertical feed-through was applied by using through glass via (TGV) which is formed by sandblasting technique of cap glass wafer. The aluminum electrodes was conformally deposited on the via-hole structure of cap glass. The TGV process provides reliable electrical interconnection between Si resonator and aluminum electrodes on the cap glass without leakage or electrical disconnection through the TGV. The fabricated MEMS resonator with proposed vacuum packaging using three-layer anodic bonding process has resonance frequency and quality factor of about 16 kHz and more than 40,000, respectively.

• Journal of the Computational Structural Engineering Institute of Korea
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• v.25 no.4
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• pp.293-300
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• 2012

In this study, the delamination analysis has been implemented to investigate the initiation and propagation of crack in composite laminates composed of orthotropic materials. A simple modeling was achieved by moving nodal technique without re-meshing work when crack propagation occurred. This paper aims at achieving two specific objectives. The first is to suggest a very simple modeling scheme compared with those applied to conventional h-FEM based models. To verify the performance of the proposed model, analysis of double cantilever beams with composite materials was implemented and then the results were compared with reference values in literatures. The second one is to investigate the behavior of interior delamination problems using the proposed model. To complete these objectives, the full-discrete-layer model based on Lobatto shape functions was considered and energy release rates were calculated using three-dimensional VCCT(virtual crack closure technique) based on linear elastic fracture mechanics.

• Bulletin of the Korean Chemical Society
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• v.24 no.2
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• pp.183-188
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• 2003

The micro-patterning of multi-layered thin films containing CdS and $Ta_2O_5$ layers on ITO substrate with various structures was successfully obtained by combining three different techniques: chemical solution depositions, sol-gel, and microcontact printing (μCP) methods using octadecyltrichlorosilane (OTS) as the organic thin layer template. $Ta_2O_5$ layer was prepared by sol-gel casting and CdS one obtained by chemical solution deposition, respectively. Parallel and cross patterns of multi-layers with $Ta_2O_5$ and CdS films were fabricated additively by successive removal of OTS layer pre-formed. This study presents the designed architectures consisting of the two types of feature having horizontal dimensions of 170 ㎛ and 340 ㎛ with constant thickness ca. 150 nm of each deposited materials. The thin film lay-out of the cross-patterning is composed of four regions with chemically different layer compositions, which are confirmed by Auger electron microanalysis.

• Journal of the Korea Fashion and Costume Design Association
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• v.9 no.3
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• pp.221-230
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• 2007

This research was focused on the changes of layered techniques in Hussein Chalayan's collections. For this purpose, the layered techniques in 134 works from Chalayan's collections, 97S/S to 06S/S, which were obtained from fashion magazines and fashion internet site, were analyzed and classified into 15 groups. Layered techniques in his works apparently expressed his philosophy about deconstruction. Multilayered feeling was given through using various techniques such as one layer clothing which was showed like multi-layered clothing. Others were multi-layer clothing which felt like one layer, breaking the stereotyped line of clothing into atypical construction and using various materials or constructive lines which made the optical feeling like one layer or multi-layer clothing, and so on. These layered techniques in Chalayan's collections were differentiated into several categories, such as techniques which were revealed in every collections, techniques which were differentiated from one another collections, techniques which were continuously showed through three collections for connection with each collection, techniques which were used only for Spring/Summer season, and techniques which were uniquely showed in early collection or recent collections.

• Journal of the Korean Society of Fisheries and Ocean Technology
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• v.30 no.3
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• pp.209-219
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• 1994

The practical application of riblet to three dimensional double model, for viscous drag reduction, was studied analytically by intergal solution to three dimensional turbulent boundary layers. The case of a V-groove riblet technique on the shear stress and boundary layer velocities were incooperated in the computation of the flow over a smooth slender ship hull. As the results the possible mechanism of turbulent drag reduction by riblets are then suggested based on detailed studies of near-wall turbulence characteristics. And a turbulent boundary layer calculation scheme based on a momentum integral method was modified for the computer program. An example of the calculation results is presented.

• Korean Journal of Materials Research
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• v.30 no.9
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• pp.453-457
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• 2020

Tomb No. 1 (Donghachong) of the Buyeo Neungsan-ri Tomb complex (listed as UNESCO World Heritage Site), is a royal tomb of the Baekje Sabi Period. One wooden coffin unearthed there is an important relic of the funerary culture of the Baekje. This study examines the production techniques of gilt-bronze objects attached to the wooden coffin excavated from Donghachong. The base metal of the gilt-bronze object is pure copper, with single α phase crystals in a heterogeneous form containing annealing twins; Au and Hg are detected in the gilt layer. We suggest that the surface of the forging copperplate is gilded using a mercury amalgam technique; it is thought that the annealing twins of the base material formed during the heat treatment process for the sheet metal. The gilt layer is three to five times thicker for the gilt-bronze objects found near the foot of the coffin than those near the head. We estimate the plating process is carried out at least three times because three layers are identified on the plate near the head. Therefore, it is likely that the materials and methods used to construct the gilt-bronze objects found in different parts of the coffin are the same, but the number of platings is different. This research confirms the metal crafting techniques used in Baekje by the examination of production techniques of these gilt-bronze objects. Further, our paper presents an important example of restoration and reconstruction for a museum exhibition, through effective use of scientific analysis and investigation.

• Current Photovoltaic Research
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• v.5 no.1
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• pp.20-24
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• 2017

Recently, three-dimensional (3D) light harvesting structures are highly attracted because of their high light harvesting capacity and charge collection efficiencies. In this study, we have fabricated $Cu_2ZnSn(S_xSe_{1-x})_4$ based 3D thin film solar cells on PR patterned Molybdenum (Mo) substrates using photolithography technique. Specifically, Mo patterns were deposited on PR patterned Mo substrates by sputtering and the thin Cu-Zn-Sn stacked layer was deposited over this Mo patterns by sputtering technique. The stacked Zn-Sn-Cu precursor thin films were sulfo-selenized to form CZTSSe pattern. Finally, CZTSSe absorbers were coated with thin CdS layer using chemical bath deposition and ZnO window layer was deposited over CZTSSe/CdS using DC sputtering technique. Fabricated 3-D solar cells were characterized by X-ray diffraction (XRD), X-ray fluorescence (XRF) analysis, Field-emission scanning electron microscopy (FE-SEM) to study their structural, compositional and morphological properties, respectively. The 3% efficiency is achieved for this kind of solar cell. Further efforts will be carried out to improve the performance of solar cell through various optimizations.

• Transactions of the Korean Society of Mechanical Engineers B
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• v.26 no.6
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• pp.893-901
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• 2002

Effect of boundary layer thickness on the flow characteristics around a rectangular prism has been investigated by using a PIV(Particle Image Velocimetry) technique. Three different boundary layers(thick, medium and thin)were generated in the Atmospheric Boundary Layer Wind Tunnel at Pusan National University. The thick boundary layer having 670 mm thickness was generated by using spires and roughness elements. The medium thickness of boundary layer($\delta$=270 mm) was the natural turbulent boundary layer at the test section floor with fairly long developing length(18 m). The thin boundary layer($\delta$=36.5 mm) was generated on the smooth panel elevated 70cm from the wind tunnel floor. The Reynolds number based on the free stream velocity(3 ㎧) and the height of the model(40 mm) was 7.9$\times$10$^3$. The mean velocity vector fields and turbulent kinetic energy distributions were measured and compared. The effect of boundary layer thickness was clearly observed not only in the length of separation bubble but also in the location of reattachment point. The thinner the boundary layer thickness, the higher the turbulent kinetic energy Peak around the model roofbecame. It is strongly recommended that the height ratio between the model and the approaching boundary layer thickness should be encountered as a major parameter.