• Journal of Plant Biotechnology
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• 제40권1호
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• pp.43-48
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• 2013

Oreorchis coreana Finet is threatened globally by over-collection from its natural habitats for horticultural purposes. Its rarity in nature makes this plant one of the most endangered species in Korea. In this study, we investigated the effects of sodium hypochlorite (NaOCl) on orchid seed viability and seed germination. An in vitro bioassay swelling test using immature seeds was compared with a standard chemical procedure using triphenyl tetrazolium chloride (TTC) to test seed viability. In general, the bioassay was more appropriate for estimating embryo viability after a prolonged pre-treatment (more than 1 h) in 1% NaOCl, a surface sterilant often used to enhance germination of seeds of terrestrial plants. Therefore, an efficient method for investigating in vitro swelling of immature seeds is urgently needed. We established a method for determining the viability and swelling of O. coreana seeds via in vitro examination of immature seeds. Treatment of immature seeds with 1% NaOCl for 10 min greatly enhanced the extent of swelling of immature zygote embryos when compared to untreated seeds. These data obtained here appear to be comparable to viability and swelling that occurs in O. coreana seeds via asymbiotic germination.

• Journal of Microbiology and Biotechnology
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• 제20권3호
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• pp.630-636
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• 2010

A survey of the endangered orchid Orchis militaris populations was carried out in north-eastern Italy. The occurrence of fungal root endophytes was investigated by light and electron microscopies and molecular techniques. Two main sites of presence were individuated in the Euganean Hills, differing as to the percentage of flowering individuals and of capsules completing maturity. Fluorescence microscopy revealed an intracellular cortical colonization by hyphal pelotons. Two ITS PCR products co-amplified. Sequencing revealed for the former an identity and a high similarity (99%) with a Tulasnellaceae (Basidiomycota) fungus found within tissues of the same host in independent studies in Hungary and Estonia, suggesting an interesting case of tight specificity throughout the Eurosiberian home range. The second amplicon had 99% similarity with Tetracladium species (Ascomycota) recently demonstrated as potential endophytes. TEM revealed two different hyphal structures. Double fungal colonization appears to occur in Orchis militaris and the possible requirement of a specific fungal partner throws light on the causes of this plant's rarity and threatened status.

• 대한원격탐사학회:학술대회논문집
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• 대한원격탐사학회 2003년도 Proceedings of ACRS 2003 ISRS
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• pp.1018-1020
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• 2003

The costal area where takes up over 32% in domestic teritory is considered as very importantly because it has not only economic facilities such as harbor and an industrial complex but also recreation facilities. Moreover, the tidal flat area has been used as culture ponds and salt farms because this area is occupied by various oceanic species. Also, the tidal flat area has played an important role to purify ocean pollution and maintain an ecosystem. However, the costal ecosystem has seriously threatened by domestic reclamation projects and a large-scale tide embankment during recent 10 years in Korea. This serious problem results in loosing 34%(810$km^2$) of the entire domestic costal area. In this paper, the micro-landform in the tidal flat area, which is called as Garolim bay in Korea, is classified by using Landsat TM images also verified through a filed report. Through the result of this, the tidal flat area is expected to manage efficiently especially through analyzing sediment environment and characteristic of grain size by using satellite images.

• 한국산림과학회지
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• 제95권5호
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• pp.532-538
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• 2006

Triphala is an important combination of three important forest fruits, i.e., Emblica officinalis, Terminalia chebula and Terminalia bellirica. Chittagong region in Bangladesh was once rich in triphala trees presently subject to the depletion. Thus, these forest resources are being threatened day by day. The study was conducted to learn the present using pattern of the triphala, causes of its depletion and the conservation strategies agreed by the villagers. Eight major uses of triphala trees were recognized. It was revealed that 100% respondents used the triphala as fruit tree followed by 71-78% as fuelwod. The present status of growing stock of triphala was found in depleted condition particularly in the Muslim dominated area. It was found that unawareness was the major cause for depleting the triphala trees agreed by the 87% respondents followed by depleting the village groves by 84%. Awareness creation (100%) and induction of social forestry program (92%) were found major recomendations by the vilagers to retard depleting the triphala tree species in the Chittagong region of Bangladesh.

• Journal of Forest and Environmental Science
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• 제36권4호
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• pp.267-273
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• 2020

Diospyros mespiliformis is a highly valued and threatened tree species within the Sahelo-Sudanian zone of Africa, but its seed germination requirements under cultivation are not well researched. In a first experiment which aimed at determining germination response of seeds to dehydration, fresh seeds were dried at room temperature for 26 days during which their moisture content, their germinability, and their viability were monitored at two-day intervals. In the second experiment, 14 pre-germination treatments were tested for their effect on the germination of dried seeds. Results showed that fresh seeds had 52.7% moisture and achieved 97.7% germination. As seeds were dried, percentage germination gradually decreased with decreasing moisture content and reached 0% when moisture content had dropped to 18%. Meanwhile, seed viability remained at 100% over drying duration. Seeds that were not germinated after air dry also recorded 100% viability. The most effective treatment for inducing germination of dried seeds was scarification using 98% sulfuric acid for 30 min which resulted in 96.6% germination. This study reports for the first time in D. mespiliformis seeds a desiccation-induced dormancy which can be efficiently alleviated by acid scarification. This study provides useful information that will contribute to efficient management of D. mespiliformis seed resources for propagation.

• 한국양봉학회지
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• 제34권2호
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• pp.125-129
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• 2019

Honey bees are important pollinators in agriculture, but are threatened by the pathogen Ascosphaera apis, which causes chalkbrood. Despite attempts to control this fungus using synthetic fungicides, none of them have been proven to be completely effective. Among 640 natural compounds that we tested, 4-hydroxyderricin (MIC=3.125, 6.25 mg/L after 24 h and 48 h growth, respectively) exhibited the strongest anti- Ascosphaera apis activity, followed by acetylshikonin (MIC=12.5 mg/L for 24 h and 48 h growth). 4-Hydroxyderricin showed selective growth inhibition of Ascosphaera apis and Rhizopus oryzae among tested fungus strains. Treatment 4-hydroxyderricin with miconazole revealed a synergistic effect (FICI=0.65±0.13 at 48 h incubation). These findings suggest that 4-hydroxyderricin, which has antifungal activity against Ascosphaera apis but few other fungal species, can effectively control infectious fungal diseases. Combined treatment of bees with 4-hydroxyderricin and miconazole could reduce cytotoxicity and improve the cost effectiveness of treatment.

• Proceedings of the National Institute of Ecology of the Republic of Korea
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• 제4권4호
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• pp.154-158
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• 2023

The Asiatic black bear, Ursus thibetanus, is among the most threatened or endangered species in Asia. For its conservation and management, sex identification of U. thibetanus using non-invasive samples (e.g., hair and/or feces) is potentially valuable. In this study, a non-invasive molecular method for sex identification of U. thibetanus samples collected from various countries was first utilized, and it was based on polymerase chain reaction (PCR) amplification of the amelogenin gene via PCRs. Thirty-three bear DNA samples, extracted not only from blood (n=9) but also from hair (n=18) and feces (n=6), were used. We performed sex-specific PCR amplifications of the amelogenin gene using a primer set, SE47 and SE48. The primer set could successfully amplify a single X-specific band for females and both X- and Y-specific bands for males from all blood (100%) and hair (100%) samples. In addition, the primer set could distinguish the sex of bears in four out of a total of six fecal samples (approximately 67%). This study's findings suggest that this molecular method can be applied to sex identification of Asiatic black bears from various Asian regions using non-invasive samples, such as hair and feces.

• 식물분류학회지
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• 제50권2호
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• pp.199-226
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• 2020

경상남도 합천군과 산청군의 경계에 위치한 황매산(1,108 m)은 소백산맥의 줄기로 식생이 풍부하고 산림생물다양성이 높다. 그러나 지속적인 개발 및 등산객 등 관광객 증가와 함께 외래식물의 유입, 식재로 인한 생태계 교란 등의 문제가 우려되고 있다. 본 연구는 황매산의 관속식물상을 파악하여 현재의 기록을 남기는 동시에 식물다양성 보존 방안 및 외래식물의 산림 내 추가유입 방지를 위한 기초자료로 활용하고자 계획되었다. 조사는 2019년 3월부터 동년 10월까지 13회에 걸쳐 계절별로 개화하는 식물을 채집하고, 기록하는 방식으로 이루어졌다. 조사 결과, 기존 조사에 비해 약 28%의 분류군이 증가한 106과 349속 554종 16아종 48변종 10품종의 총 628분류군을 확인하였다. 이는 국내 관속식물 4,364분류군의 14.4%에 해당되며, 이중 양치식물이 26분류군(4.1%), 나자식물이 5분류군(0.8%), 쌍자엽식물이 477분류군(76.0%), 단자엽식물은 120분류군(19.1%)으로 분류되었다. 조사된 분류군 중 특산식물은 16분류군, 산림청지정 희귀식물은 14분류군이었고, 침입외래식물은 42분류군, 환경부 지정 식물구계학적 등급종은 75분류군으로 나타났다.

• 한국자원식물학회지
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• 제31권2호
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• pp.149-161
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• 2018

본 연구는 덕정산의 관속식물상을 밝히고 주요 식물종을 조사하였다. 2016년 3월부터 10월까지 총 8회에 걸쳐 현지조사를 실시한 결과 관속식물은 107과 305속 423종 8아종 48변종 4품종 1교잡종으로 총 484분류군이 확인되었다. 덕정산은 식물구계학상 온대중부지역에 속하고 낙엽활엽수와 침엽수의 혼합림으로 산지의 대부분은 소나무-참나무류림이 우점하며 2차림으로 구성된다. 식물 종다양성이 높은 과는 국화과(62분류군), 화본과(48분류군), 사초과(27분류군), 콩과(23분류군), 꿀풀과(21분류군)이었다. 한반도 고유종은 외대으아리, 키버들, 좀목포사초, 백운산원추리 4분류군이 확인되었다. IUCN 평가기준에 따른 적색목록식물은 5분류군으로 준위협종(NT)에 쑥방망이, 관심대상종(LC)에 물잔디, 미평가종(NE)에 왕과, 버들잎엉겅퀴, 멱쇠채가 관찰되었다. 식물구계학적 특정식물은 V등급 1분류군, IV등급 2분류군, III등급 4분류군, II등급 8분류군, I등급 11분류군으로 총 26분류군이 발견되었다. 외래식물은 46분류군으로 귀화율 9.5%, 도시화지수 14.3%로 나타났다.

• 한국조경학회지
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• 제32권4호
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• pp.28-38
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• 2004

Korean goral (Nemorhaedus caudatus raddeanus) is one of the endangered species in Korea, and the rugged terrain of the Soraksan National Park (373㎢) is a critical habitat for the species. But the goral population is threatened by habitat fragmentation caused by roads and hiking trails. The objective of this study was to develop a potential habitat suitability model for Korean goral in the park, and the model was based on the concepts of fuzzy set theory and multi-criteria evaluation. The process of the suitability modeling could be divided into three steps. First, data for the modeling was collected by using field work and a literature survey. Collected data included 204 points of GPS data obtained through a goral trace survey and through the number of daily visitors to each hiking trail during the peak season of the park. Second, fuzzy set theory was employed for building a GIS data base related to environmental factors affecting the suitability of the goral habitat. Finally, a multiple-criteria evaluation was performed as the final step towards a goral habitat suitability model. The results of the study were as follows. First, characteristics of suitable habitats were the proximity to rock cliffs, scattered pine (Pinus densiflora) patches, ridges, the elevation of 700∼800m, and the aspect of south and southeast. Second, the habitat suitability model had a high classification accuracy of 93.9％ for the analysis site, and 95.7％ for the validation site at a cut off value of 0.5. Finally, 11.7％ of habitatwith more than 0.5 of habitat suitability index was affected by roads and hiking trails in the park.