• 제목/요약/키워드: Threat Response Center

검색결과 47건 처리시간 0.025초

항원 특이적 단일 기억 B 세포 분리를 이용한 신종 바이러스 대응 신속 항체 플랫폼 개발 (Development of Rapid Antibody-based Therapeutic Platform Correspondence for New Viruses Using Antigen-specific Single Cell Memory B Cell Sorting Technology)

  • 석지윤;정수한;한예지;박아름;김정은;송영조;유치호;윤형석;구세훈;이승호;이용한;허경행;최웅
    • 한국군사과학기술학회지
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    • 제27권1호
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    • pp.116-125
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    • 2024
  • The COVID-19 pandemic is not over despite the emergency use authorization as can see recent COVID-19 daily confirmed cases. The viruses are not only difficult to diagnose and treat due to random mutations, but also pose threat human being because they have the potential to be exploited as biochemical weapons by genetic manipulation. Therefore, it is inevitable to the rapid antibody-based therapeutic platform to quickly respond to future pandemics by new/re-emerging viruses. Although numerous researches have been conducted for the fast development of antibody-based therapeutics, it is sometimes hard to respond rapidly to new viruses because of complicated expression or purification processes for antibody production. In this study, a novel rapid antibody-based therapeutic platform using single B cell sorting method and mRNA-antibody. High immunogenicity was caused to produce antibodies in vivo through mRNA-antigen inoculation. Subsequently, antigen-specific antibody candidates were selected and obtained using isolation of B cells containing antibody at the single cell level. Using the antibody-based therapeutic platform system in this study, it was confirmed that novel antigen-specific antibodies could be obtained in about 40 days, and suggested that the possibility of rapid response to new variant viruses.

한국 마을숲 생태계 취약요소 발굴 및 취약성 평가 (The Development of Vulnerable Elements and Assessment of Vulnerability of Maeul-soop Ecosystem in Korea)

  • 임정철;류태복;안경환;최병기
    • 한국전통조경학회지
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    • 제34권4호
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    • pp.57-65
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    • 2016
  • 마을숲은 역사적, 문화적으로 한국 전통 마을경관의 핵심요소이다. 그러나 근대 이후 다양한 영향으로 많은 수의 마을숲이 소실되었거나 쇠퇴되고 있는 실정이다. 역사적, 문화적, 생태적 다양한 보전가치를 가진 마을숲의 체계적 보전 및 복원을 위한 관심과 노력이 필요한 시점이다. 본 연구는 한국 전역에 분포하고 있는 499개의 마을숲에 대하여 구성 식물종 및 서식처, 입지 특성 등을 바탕으로 국내 마을숲에 대한 생태학적 복원 및 지속가능한 이용과 관리 등에 관한 정보를 제공하고자 하였다. 마을숲 생태계를 위협하는 대표적인 6가지 범주의 위협요인을 발굴하고 각 요인별 영향력을 평가하였다. 마을숲 생태계 취약성에 미치는 영향의 정도에 대해 위협요인별 가중치 평가를 AHP 분석기법을 이용하여 보다 입체적으로 분석하였다. AHP 분석기법을 이용한 평가결과 6가지 범주 가운데 면적 축소가 국내 마을숲의 존립에 가장 큰 위협요인으로 지목되었다. 그 다음으로 지형, 토양환경 변화가 마을숲 생태계를 질적으로 변화시키는 위협 요인으로 고려되었다. 식생의 구조와 질적 변화는 서식처 변화보다 마을숲 소실 및 쇠퇴에 낮은 영향을 미칠 것으로 평가되었다. 각 요인별 가중치를 바탕으로 100점을 최고점으로 수치를 환산한 평가틀을 이용하여 마을숲에 대한 취약성 평가가 이루어졌다. 국내 마을숲의 취약성 평가결과 가장 빈도 높은 값을 보이는 등급은 III등급이며, 낮은 등급에서 높은 등급으로 정규분포 경향을 보였다. 높은 자연성을 보이는 I등급의 마을숲이 38개소 존재했으며, 취약요인들에 의해 마을숲 존폐를 위협받는 V등급의 마을숲도 10개소 확인되었다. 각 마을숲별 취약성 평가결과에서도 국내 마을숲의 지속가능성 보장을 위해 마을숲 고유의 면적 회복이 최우선 과제로 대두되었다. 국가 중요 생태자원으로서 각각의 취약요소별 국가수준의 생태적 대응전략 마련이 필요한 것으로 확인되었다.

Evaluation and Verification of Barley Genotypes with Known Genes for Resistance to Barley yellow mosaic virus and Barley mild mosaic virus Under Field Conditions in South Korea

  • Kim, Hong-Sik;Baek, Seong-Bum;Kim, Dea-Wook;Hwang, Jong-Jin;Kim, Si-Ju
    • The Plant Pathology Journal
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    • 제27권4호
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    • pp.324-332
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    • 2011
  • Soil-borne barley yellow mosaic disease caused by Barley yellow mosaic virus (BaYMV) or Barley mild mosaic virus (BaMMV) gives a serious threat to the winter barley cultivated in the southern regions in Korea. It is important to develop resistant varieties for stable and high-yield production. The objectives of this study were to evaluate 22 genotypes of exotic barley germplasms carrying the resistance genes rym1 through rym12, with the exception of rym10, and to determine the genes that confer resistance to BaYMV or BaMMV in Korea. Using the traditional visual scoring of symptoms at 4 locations over 3 years, average disease rate values differed (P < 0.001) among the genotypes. ELISA test revealed the presence of both BaYMV and BaMMV in all of the field sites but Jinju and significantly different rates of infection among genotypes and years. Barley genotypes differed in how virus quantities and pathogen-induced symptoms were correlated, especially in response to BaYMV. Disease incidence was affected by the climatic conditions present during the early growing stage before overwintering. A Chinese landrace, 'Mokusekko 3', carrying rym1 and rym5 was comparatively resistant at all locations studied. The barley genotypes carrying either rym6 or rym9 were susceptible to the viral strains. The genotypes carrying rym5 were resistant in Jinju and Milyang but susceptible in Iksan and Naju. The resistance genes rym2 and rym3 were effective in local strains and would be potent contributors to disease resistance.

고체로켓모터의 비선형 연소 불안정성 예측 기법 (The Nonlinear Combustion Instability Prediction of Solid Rocket Motors)

  • 홍지석;문희장;성홍계;엄원석;서성현;이도형
    • 한국추진공학회지
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    • 제20권1호
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    • pp.20-27
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    • 2016
  • 연소 불안정 현상은 연료의 응답 함수, 연소율, 국부 연료 표면의 유동 속도에 영향을 미치기 때문에 고체로켓 모터 설계 시 성능이나 구조적 안정성을 다루는 측면에서 매우 중요한 부분이다. 연소 불안정 현상은 음향 모드가 연소/유동과정에서 발생하는 진동수와 결합(coupled)되었을 때 발생한다. 본 연구에서는 비선형 파동방정식으로부터 음향 비선형 불안정 모델을 유도하여 실린더형 고체로켓 모터의 연소 불안정 현상을 분석하였다. 또한 고체로켓 모터 연소실 압력, 연소 속도가 연소 불안정에 미치는 영향을 조사하였다.

통합 사이버 보안 상황분석을 통한 관제 상황인지 기술 (Context cognition technology through integrated cyber security context analysis)

  • 남승수;서창호;이주영;김종현;김익균
    • 스마트미디어저널
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    • 제4권4호
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    • pp.80-85
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    • 2015
  • 인터넷을 이용하는 응용의 수가 급격히 증가함에 따라 인터넷 상에서 이루어지는 사이버 공격의 발생 빈도는 점점 증가하고 있다. 전 세계적으로 L3 DDoS 공격 탐지 중비의 장비에서 응용계층 기반의 지능형 DDoS 공격에 대한 탐지가 미비하다. 차세대 네트워크 보안솔루션의 다양한 요구사항을 충족할 수 있는 고성능 유무선 네트워크 위협대응 기술에 있어서 국내제품은 외국제품에 비해 기능면에서는 근접하나 성능은 미비한 상황에 있으며, 악성코드 탐지 및 시그니쳐 생성연구 관련하여 주로 Window OS에서 동작하는 악성코드 탐지 및 분석 연구 중심으로 진행하고 있다. 본 논문에서는 최신 사이버 보안 상황 침해공격 분석을 통한 최신 다양한 신종 공격 기법 및 분석 기술의 현황 조사, 분석등을 기술한다.

통합 사이버 보안 상황분석을 통한 관제 상황인지 기술 (Context cognition technology through integrated cyber security context analysis)

  • 남승수;서창호;이주영;김종현;김익균
    • 디지털융복합연구
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    • 제13권1호
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    • pp.313-319
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    • 2015
  • 인터넷을 이용하는 응용의 수가 급격히 증가함에 따라 인터넷 상에서 이루어지는 사이버 공격의 발생 빈도는 점점 증가하고 있다. 전 세계적으로 L3 DDoS 공격 탐지 중비의 장비에서 응용계층 기반의 지능형 DDoS 공격에 대한 탐지가 미비하다. 차세대 네트워크 보안솔루션의 다양한 요구사항을 충족할 수 있는 고성능 유무선 네트워크 위협대응 기술에 있어서 국내제품은 외국제품에 비해 기능면에서는 근접하나 성능은 미비한 상황에 있으며, 악성코드 탐지 및 시그니쳐 생성연구 관련하여 주로 Window OS에서 동작하는 악성코드 탐지 및 분석 연구 중심으로 진행하고 있다. 본 논문에서는 최신 사이버 보안 상황 침해 공격 분석을 통한 최신 다양한 신종 공격 기법 및 분석 기술의 현황 조사, 분석등을 기술한다.

A Small GTPase RHO2 Plays an Important Role in Pre-infection Development in the Rice Blast Pathogen Magnaporthe oryzae

  • Fu, Teng;Kim, Joon-Oh;Han, Joon-Hee;Gumilang, Adiyantara;Lee, Yong-Hwan;Kim, Kyoung Su
    • The Plant Pathology Journal
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    • 제34권6호
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    • pp.470-479
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    • 2018
  • The rice blast pathogen Magnaporthe oryzae is a global threat to rice production. Here we characterized RHO2 gene (MGG_02457) that belongs to the Rho GTPase family, using a deletion mutant. This mutant ${\Delta}Morho2$ exhibited no defects in conidiation and germination but developed only 6% of appressoria in response to a hydrophobic surface when compared to the wild-type progenitor. This result indicates that MoRHO2 plays a role in appressorium development. Furthermore, exogenous cAMP treatment on the mutant led to appressoria that exhibited abnormal morphology on both hydrophobic and hydrophilic surfaces. These outcomes suggested the involvement of MoRHO2 in cAMP-mediated appressorium development. ${\Delta}Morho2$ mutation also delayed the development of appressorium-like structures (ALS) at hyphal tips on hydrophobic surface, which were also abnormally shaped. These results suggested that MoRHO2 is involved in morphological development of appressoria and ALS from conidia and hyphae, respectively. As expected, ${\Delta}Morho2$ mutant was defective in plant penetration, but was still able to cause lesions, albeit at a reduced rate on wounded plants. These results implied that MoRHO2 plays a role in M. oryzae virulence as well.

Phylogeny, Morphology and Pathogenicity of Biscogniauxia mediterranea Causing Charcoal Canker Disease on Quercus brantii in Southern Iran

  • Samaneh, Ahmadi;Fariba, Ghaderi;Habiballah, Charehgani;Soraya, Karami;Dariush, Safaee
    • 식물병연구
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    • 제28권4호
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    • pp.209-220
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    • 2022
  • Charcoal canker of oak, which has recently increased in southern Iran, could pose a serious threat to the entire forest ecosystem in the near future. In addition, it seems that climate change and its consequences, such as drought in the southern regions of Iran, have exacerbated this phenomenon. Consequently, the objective of this study was to identify the fungal pathogens that could cause charcoal canker disease in the oak forests of South Zagros. It was also sought to find associations between changes in the occurrence/exacerbation of charcoal canker disease under non and intense drought stress in non-inoculated or inoculated Quercus brantii seedlings. In total, 120 isolates were obtained from eight oak forests located in the Zagros Mountains of Southern Iran, Kohgiluyeh & Boyer-Ahmad and Fars provinces, which were classified as Biscogniauxia mediterranea based on morphological assessment. Subsequently, molecular assay confirmed the result by phylogenetic inference of internal transcribed spacer-rDNA regions, α-actin, and β-tubulin genes. The results of the pathogenicity test showed that the response of isolates of B. mediterranea (Iran-G1 and Iran-M70) was varied in different environments for the measured necrotic lesion length. In comparison with the control moisture treatments (non-stress), the necrotic lesion length in inoculated treatments increased under intense drought stress. In general, inoculated oak seedlings' exposure to water-deficient stress by the pathogen of B. mediterranea could affect the spread/severity of the charcoal canker disease.

Painful Channels in Sensory Neurons

  • Lee, Yunjong;Lee, Chang-Hun;Oh, Uhtaek
    • Molecules and Cells
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    • 제20권3호
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    • pp.315-324
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    • 2005
  • Pain is an unpleasant sensation experienced when tissues are damaged. Thus, pain sensation in some way protects body from imminent threat or injury. Peripheral sensory nerves innervated to peripheral tissues initially respond to multiple forms of noxious or strong stimuli, such as heat, mechanical and chemical stimuli. In response to these stimuli, electrical signals for conducting the nociceptive neural signals through axons are generated. These action potentials are then conveyed to specific areas in the spinal cord and in the brain. Sensory afferent fibers are heterogeneous in many aspects. For example, sensory nerves are classified as $A{\alpha}$, $-{\beta}$, $-{\delta}$ and C-fibers according to their diameter and degree of myelination. It is widely accepted that small sensory fibers tend to respond to vigorous or noxious stimuli and related to nociception. Thus these fibers are specifically called nociceptors. Most of nociceptors respond to noxious mechanical stimuli and heat. In addition, these sensory fibers also respond to chemical stimuli [Davis et al. (1993)] such as capsaicin. Thus, nociceptors are considered polymodal. Recent advance in research on ion channels in sensory neurons reveals molecular mechanisms underlying how various types of stimuli can be transduced to neural signals transmitted to the brain for pain perception. In particular, electrophysiological studies on ion channels characterize biophysical properties of ion channels in sensory neurons. Furthermore, molecular biology leads to identification of genetic structures as well as molecular properties of ion channels in sensory neurons. These ion channels are expressed in axon terminals as well as in cell soma. When these channels are activated, inward currents or outward currents are generated, which will lead to depolarization or hyperpolarization of the membrane causing increased or decreased excitability of sensory neurons. In order to depolarize the membrane of nerve terminals, either inward currents should be generated or outward currents should be inhibited. So far, many cationic channels that are responsible for the excitation of sensory neurons are introduced recently. Activation of these channels in sensory neurons is evidently critical to the generation of nociceptive signals. The main channels responsible for inward membrane currents in nociceptors are voltage-activated sodium and calcium channels, while outward current is carried mainly by potassium ions. In addition, activation of non-selective cation channels is also responsible for the excitation of sensory neurons. Thus, excitability of neurons can be controlled by regulating expression or by modulating activity of these channels.

중국군의 해양작전능력과 한국군의 과제 (PRC Maritime Operational Capability and the Task for the ROK Military)

  • 김민석
    • Strategy21
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    • 통권33호
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    • pp.65-112
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    • 2014
  • Recent trends show that the PRC has stepped aside its "army-centered approach" and placed greater emphasis on its Navy and Air Force for a wider range of operations, thereby reducing its ground force and harnessing its economic power and military technology into naval development. A quantitative growth of the PLA Navy itself is no surprise as this is not a recent phenomenon. Now is the time to pay closer attention to the level of PRC naval force's performance and the extent of its warfighting capacity in the maritime domain. It is also worth asking what China can do with its widening naval power foundation. In short, it is time to delve into several possible scenarios I which the PRC poses a real threat. With this in mind, in Section Two the paper seeks to observe the construction progress of PRC's naval power and its future prospects up to the year 2020, and categorize time frame according to its major force improvement trends. By analyzing qualitative improvements made over time, such as the scale of investment and the number of ships compared to increase in displacement (tonnage), this paper attempts to identify salient features in the construction of naval power. Chapter Three sets out performance evaluation on each type of PRC naval ships as well as capabilities of the Navy, Air Force, the Second Artillery (i.e., strategic missile forces) and satellites that could support maritime warfare. Finall, the concluding chapter estimates the PRC's maritime warfighting capability as anticipated in respective conflict scenarios, and considers its impact on the Korean Peninsula and proposes the directions ROK should steer in response. First of all, since the 1980s the PRC navy has undergone transitions as the focus of its military strategic outlook shifted from ground warfare to maritime warfare, and within 30 years of its effort to construct naval power while greatly reducing the size of its ground forces, the PRC has succeeded in building its naval power next to the U.S.'s in the world in terms of number, with acquisition of an aircraft carrier, Chinese-version of the Aegis, submarines and so on. The PRC also enjoys great potentials to qualitatively develop its forces such as indigenous aircraft carriers, next-generation strategic submarines, next-generation destroyers and so forth, which is possible because the PRC has accumulated its independent production capabilities in the process of its 30-year-long efforts. Secondly, one could argue that ROK still has its chances of coping with the PRC in naval power since, despite its continuous efforts, many estimate that the PRC naval force is roughly ten or more years behind that of superpowers such as the U.S., on areas including radar detection capability, EW capability, C4I and data-link systems, doctrines on force employment as well as tactics, and such gap cannot be easily overcome. The most probable scenarios involving the PRC in sea areas surrounding the Korean Peninsula are: first, upon the outbreak of war in the peninsula, the PRC may pursue military intervention through sea, thereby undermining efforts of the ROK-U.S. combined operations; second, ROK-PRC or PRC-Japan conflicts over maritime jurisdiction or ownership over the Senkaku/Diaoyu islands could inflict damage to ROK territorial sovereignty or economic gains. The PRC would likely attempt to resolve the conflict employing blitzkrieg tactics before U.S. forces arrive on the scene, while at the same time delaying and denying access of the incoming U.S. forces. If this proves unattainable, the PRC could take a course of action adopting "long-term attrition warfare," thus weakening its enemy's sustainability. All in all, thiss paper makes three proposals on how the ROK should respond. First, modern warfare as well as the emergent future warfare demonstrates that the center stage of battle is no longer the domestic territory, but rather further away into the sea and space. In this respect, the ROKN should take advantage of the distinct feature of battle space on the peninsula, which is surrounded by the seas, and obtain capabilities to intercept more than 50 percent of the enemy's ballistic missiles, including those of North Korea. In tandem with this capacity, employment of a large scale of UAV/F Carrier for Kill Chain operations should enhance effectiveness. This is because conditions are more favorable to defend from sea, on matters concerning accuracy rates against enemy targets, minimized threat of friendly damage, and cost effectiveness. Second, to maintain readiness for a North Korean crisis where timely deployment of US forces is not possible, the ROKN ought to obtain capabilities to hold the enemy attack at bay while deterring PRC naval intervention. It is also argued that ROKN should strengthen its power so as to protect national interests in the seas surrounding the peninsula without support from the USN, should ROK-PRC or ROK-Japan conflict arise concerning maritime jurisprudence. Third, the ROK should fortify infrastructures for independent construction of naval power and expand its R&D efforts, and for this purpose, the ROK should make the most of the advantages stemming from the ROK-U.S. alliance inducing active support from the United States. The rationale behind this argument is that while it is strategically effective to rely on alliance or jump on the bandwagon, the ultimate goal is always to acquire an independent response capability as much as possible.