• Title/Summary/Keyword: Thermal Profile

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In Situ PCR on the Glass Slide Using the Conventional DNA Thermal Cycler (기존의 핵산증폭기를 사용한 유리슬라이드상에서의 원위치 중합효소 연쇄반응)

  • 오정균;장진수;이재영
    • Korean Journal of Microbiology
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    • v.39 no.3
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    • pp.197-200
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    • 2003
  • In order to establish effective in situ PCR on the glass slide using the conventional DNA thermal cycler, several parameters should be considered. These include full accessibility of PCR reagents into the cells, prevention of diffusing PCR products out of the cells, loss of PCR reagents by nonspecific adherence onto the glass slide, dryness of PCR reagents by heat, and heat conductivity from the heat block to the glass slide. Especially, to guarantee the full accessibility of PCR reagents to sample, relatively higher concentration of PCR reagents (particularly 4.5 mM of $Mg^{++}$) was required while 5 to 10 units/50 ${\mu}l$ reaction of Taq enzyme was enough as long as the step of pre-PCR incubation was included. Dryness of sample was prevented by addition of distilled water into the empty slots in the heat block, thereby providing the reproducible temperature-time profile of PCR. Observed temperature was lower than the programmed temperature by 3 to $4^{\circ}C$.

Properties of colored topaz by new surface treatment (새롭게 표면처리된 유색 토파즈의 특성)

  • Lee, Bohyun;Koo, Changsik;Yeon, Seokju;Choi, Hyunmin;Kim, Youngchool;Kim, Sunhee
    • Journal of the Korean Crystal Growth and Crystal Technology
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    • v.23 no.2
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    • pp.81-85
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    • 2013
  • We performed analysis of composition and structure of coating layers by using ED-XRF and TOF-SIMS for some passion topaz of Swarovski which developed recently as new surface treatment of TCF (thermal color fusion) technique. In addition, we compared differences between Ti-coated topaz (Mystic topaz) and new treated colored topazes (passion topaz) with magnification observation and simplified durability test. As a result, we can observe similar characteristic clues in Ti-coated topaz and passion topaz by magnified observation. According to results of depth profile by TOF-SIMS, we can know that topaz is treated by multi-layer coating or surface diffusion coating. Moreover, the passion topaz which is treated by chemical reaction between metal elements shows more stable chemical resistance and higher Mohs' hardness than Ti-coated topaz.

Bora wind characteristics for engineering applications

  • Lepri, Petra;Vecenaj, Zeljko;Kozmar, Hrvoje;Grisogono, Branko
    • Wind and Structures
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    • v.24 no.6
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    • pp.579-611
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    • 2017
  • Bora is a strong, usually dry temporally and spatially transient wind that is common at the eastern Adriatic Coast and many other dynamically similar regions around the world. One of the Bora main characteristics is its gustiness, when wind velocities can reach up to five times the mean velocity. Bora often creates significant problems to traffic, structures and human life in general. In this study, Bora velocity and near-ground turbulence are studied using the results of three-level high-frequency Bora field measurements carried out on a meteorological tower near the city of Split, Croatia. These measurements are analyzed for a period from April 2010 until June 2011. This rather long period allows for making quite robust and reliable conclusions. The focus is on mean Bora velocity, turbulence intensity, Reynolds shear stress and turbulence length scale profiles, as well as on Bora velocity power spectra and thermal stratification. The results are compared with commonly used empirical laws and recommendations provided in the ESDU 85020 wind engineering standard to question its applicability to Bora. The obtained results report some interesting findings. In particular, the empirical power- and logarithmic laws proved to fit mean Bora velocity profiles well. With decreasing Bora velocity there is an increase in the power-law exponent and aerodynamic surface roughness length, and simultaneously a decrease in friction velocity. This indicates an urban-like velocity profile for smaller wind velocities and a rural-like velocity profile for larger wind velocities. Bora proved to be near-neutral thermally stratified. Turbulence intensity and lateral component of turbulence length scales agree well with ESDU 85020 for this particular terrain type. Longitudinal and vertical turbulence length scales, Reynolds shear stress and velocity power spectra differ considerably from ESDU 85020. This may have significant implications on calculations of Bora wind loads on structures.

Improvement of Thermal Stability of Ni-Silicide Using Vacuum Annealing on Boron Cluster Implanted Ultra Shallow Source/Drain for Nano-Scale CMOSFETs

  • Shin, Hong-Sik;Oh, Se-Kyung;Kang, Min-Ho;Lee, Ga-Won;Lee, Hi-Deok
    • JSTS:Journal of Semiconductor Technology and Science
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    • v.10 no.4
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    • pp.260-264
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    • 2010
  • In this paper, Ni silicide is formed on boron cluster ($B_{18}H_{22}$) implanted source/drains for shallow junctions of nano-scale CMOSFETs and its thermal stability is improved, using vacuum annealing. Although Ni silicide on $B_{18}H_{22}$ implanted Si substrate exhibited greater sheet resistance than on the $BF_2$ implanted one, its thermal stability was greatly improved using vacuum annealing. Moreover, the boron depth profile, using vacuum post-silicidation annealing, showed a shallower junction than that using $N_2$ annealing.

A Study for Prediction of Water Contents in Soil by Using the Soil Thermal Conductivity (토양 열전도를 이용한 토양함수비 예측에 관한 연구)

  • Cho, Jin-Woo;Kang, Do-Kyung;Kang, E-Sok
    • Journal of the Korea Institute of Military Science and Technology
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    • v.18 no.2
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    • pp.125-130
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    • 2015
  • Vehicles and UGV(Unmanned Ground Vehicle) need a variety of road informations, such as road profile, soil type and soil water contents, to run a cross country course. Especially, soil water contents are very important factor to judge the vehicle mobility, because it can change soil strength. This paper describes the real-time measuring method of soil water contents by using the soil thermal conductivity.

A training of SMA wire for stabilization of two-way behaviors and actuator application (형상기억합금 와이어의 거동 안정화를 위한 트레이닝과 작동기 응용)

  • Kim, Sang-Haun;Yang, Sung-Pil;Cho, Maeng-Hyo
    • Proceedings of the Korean Society for Noise and Vibration Engineering Conference
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    • 2007.05a
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    • pp.924-927
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    • 2007
  • In this study, adaptation of two-way shape memory effect of SMA wire to the actuator is examined. Therefore the SMA characteristics which are training, material properties, response time at different thermal cycling rates are tested. During training, permanent deformation is accumulated till a certain number of cycle and then saturated. The amount of two-way strain is unchangeable over all cycle and the slope of strain(or stress)-temperature curve is slower as the increase of applied stress. The rate effect is observed resulted from the thermal distribution which heating profile differs from cooling as thermal cycling time. Using the estimated SMA properties, an experimental test for the simple smart wing is performed.

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Thermal Analysis of Mica/Epoxy Composites used In Generator Stator Windings (발전기 고정자 권선에서 사용되는 마이카/에폭시의 열적 분석)

  • 김희동;김태완;김정훈
    • Proceedings of the Korean Institute of Electrical and Electronic Material Engineers Conference
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    • 1997.04a
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    • pp.330-333
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    • 1997
  • The thermal impact of mica/epoxy paper(130${\mu}{\textrm}{m}$) is investigated using XRD, DSC and TGL X-ray diffraction(XRD) analysis was performed to know the position and structure of mica crystal in insulation materials. A differential scanning calorimeter(DSC) was used to measure glass transition temperature and excess enthalphy of the composite materials that had been subjected to thermal aging. The glass transition temperature(T$_{g}$) measured by DSC is observed at 95.43$^{\circ}C$ and 113.43$^{\circ}C$, respectively. The T$_{g}$ also increases with increased aging time. Measurements performed by TGA(thermogravimetric analysis) have showed that weight loss profile of sound specimens are lower than those aged.ged.

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Experimental Test Numerical Simulation of SMA Characteristics and Device verification (형상기억합금 수치해석을 위한 특성 실험 및 작동기 응용)

  • Kim, Sang-Haun;Choi, Hyun-Ho;Cho, Maeng-Hyo
    • Proceedings of the Korean Society For Composite Materials Conference
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    • 2005.11a
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    • pp.145-148
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    • 2005
  • In this study, adaptation of two-way shape memory effect of SMA wire to the actuator is examined . Therefore the SMA characteristics which are training, material properties, response time at different thermal cycling rates are tested. During training, permanent deformation is accumulated till a certain number of cycle and then saturated. The amow1t of two-way strain is unchangeable over all cycle and the slope of strain(or stress)-temperature curve is slower as the increase of applied stress. The rate effect is observed resulted from the thermal distribution which heating profile differs from cooling as thermal cycling time. Using the estimated SMA properties, an experimental test for the simple smart wing is performed.

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Fabrication and Characteristics of Microlens using Thermal Reflow Method (Thermal Reflow법에 의한 마이크로렌즈의 제작 및 그 특성)

  • 박광범;김인회;정석원;김건년;문현찬;박효덕;신상모
    • Proceedings of the Optical Society of Korea Conference
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    • 2000.02a
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    • pp.192-193
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    • 2000
  • We studied the characteristics and fabricated the plano-convex refractive microlenses using the thermal reflow method. The exposed resist was resolved in a standard developing process. The remaining resist of circle pattern was melted in an oven 12$0^{\circ}C$ to 15$0^{\circ}C$. The shape of the melted resist microlenses is ruled by surface tension. Diameter and hight of the fabricated microlenses were 250${\mu}{\textrm}{m}$ to 325${\mu}{\textrm}{m}$ and 15${\mu}{\textrm}{m}$ to 22${\mu}{\textrm}{m}$, respectively. The surface profile was calculated using data curve-fitting method with circle equation. The optical characteristics was analysed using optical simulation program.

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Observing Thermal Counterflow in He II by the Particle Image Velocimetry Technique

  • Van Sciver S. W.
    • Progress in Superconductivity and Cryogenics
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    • v.7 no.2
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    • pp.1-6
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    • 2005
  • The Particle Image Velocimetry (PIV) technique can be used to obtain a whole-field view of thermal counterflow velocity profile in He II. Using commercially available microspheres, we have been able to visualize the normal fluid velocity in He II thermal counterflow; however, the measured velocities are less than predicted from the two fluid model. None the less, the PIV is a useful tool for observing the counterflow field in He II flow, particularly where the flow is complex as occurs through channel constrictions or around bluff objects. The present paper shows recent results using PIV to observe He II counterflow. Two cases are discussed: 1D channel flow and turbulent flow around a circular cylinder.