• Journal of Korean Society of Forest Science
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• v.84 no.1
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• pp.87-96
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• 1995

We examined the effects of seed treatment on field germination rate and seedling growth for four woody species, Euonymus alatus, Thea sinensis, Zanthoxylum piperitum, Nandina domestia which are economically useful in Korea. The seeds of each species were purified carefully after collection during Oct. and Nov.. E. alatus, T. sinensis, and Z. piperitum were sowed in the experimental field after stored in open ground with treatment of Pon-Pon. $H_2O_2$, $GA_3$, scarification. and N. domestica was sowed in container within green house after treatment of three different periods(7, 15, 21days) in growth chamber(4, 25, $32^{\circ}C$). Germination rate and seedling growth were measured in mid-Oct.. In E. alatus germination rate was higher in storaging in open ground after soaking in $GA_3$(67.1%) than in only storaging in open ground(18.4%). But the rate in T. sinensis showed no differences between storage in open ground after treatment of $GA_3$, and scarification, and only storage in open ground, Germination rate of Z. piperitum was much higher in storaging in open ground after treatment of Pon-Pon(80.3%) than in only storaging in open ground(12.4%). In N. domestica. seeds stored for 7 days at $32^{\circ}C$ were germinated faster than those stored for 7 days at $4^{\circ}C$, and germination rate of the former was also higher than that of the latter. Peak Times of seed germination in field were order of E. alatus (32 days). Z. piperitum (49 days). T. sinensis (83 days), N. domestica (87 days). The growth of seedling germinated in field showed a good result because of early germination following the effect of seed treatment. The periods of highest growth performances of the seedlings were 72 days in late Jun.-mid Jul. in E. alatus. 59 days in late Jun.- mid Jul. in Z. Piperitum, and 45 days in mid Aug.- mid Sep. in T. sinensis.

• Korean Journal of Medicinal Crop Science
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• v.13 no.1
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• pp.11-16
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• 2005

Soybean sprouts presoaked to leaf extracts were on sale as taking a growing concern about wellbeing foods. The study was carried out to compare the effects of culture period after presoaking tea (Thea sinensis L.) and hard rubber tree (Eucommia ulmoides Oliver) leaf extracts on lateral root formation, growth and morphological characters of the sprouts. Seeds of three cultivars, Pungsannamulkong, Sowonkong and Junjery, were imbibed for 5 hours into 10% solutions immediately before 5, 6 and 7 day cultures. The cultured sprouts were classified into 4 categories to calculate their composition rates on the base of hypocotyllengths; >7 cm (A),4 to 7 cm (B),< 4 cm (C) and not germinated (D), and their morphological characters, fresh and dry weights were measured. Rate of A was the lowest in Junjery of the three cultivars, while that of C showed reverse result compared to A. Similar results to that of A were observed between the three cultivars in sprout length including hypocotyl and root, diameters in middle and hook parts of hypocotyl, total fresh and dry weights. Regardless of tea and hard rubber tree leaf extract treatments, longer culture periods increased rate of A due to decrements of B, C and D, and also did the growth and the morphological characters mentioned above. Although longer culture periods reduced the increment of lateral roots, the latter treatment was more delayed in their formation and less in the roots per sprout than the former one. The growth and the morphological characters above were less in the latter treatment than in the former one in general, meaning that hard rubber tree leaf extract could more repress growth of soybean sprouts than tea leaf extract.

• Applied Biological Chemistry
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• v.41 no.8
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• pp.605-607
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• 1998

A total of 28 traditional drinks derived from 23 plant species in 19 families were tested for their in vitro growth-inhibiting effects against Bifidobacterium adolescents, Bifidobacterium bifidum, Lactobacillus acidophilus, Lactobacillus casei, Clostridium perfringens, and Escherichia coil using a paper disc agar diffusion method under anaerobic condition. The responses varied with bacterial strain, plant species and tissue sampled. In a test with C. perfringens at 5 and 10 mg/disc, potent growth inhibition was produced from the extracts of Eucommia ulmoides stems, Pinus densiflora leaves and shoots, Thea sinensis leaves (green and oolong teas) and Zingiber officinale roots. All materials tested did not adversely affect the growth of bifidobacteria, lactobacilli, and E. coli. These results may be an indication of at least one of the pharmacological activities of these plant-derived drinks.

• Korean Journal of Environment and Ecology
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• v.16 no.3
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• pp.296-308
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• 2002

The vascular plants in Mt. Cheongwan, Jangheung-gun, Jeollanam-do, were consisted of 599 taxa; 117 families, 366 genus, 519 species, 76 varieties and 4 forma. From the floristic point of view, Mt. Cheongwan is distributed to both the evergreen trees as Pteris mulfida, Dryopteris bissetiana, Torreya nucifera, Cephlotaxus koreana, Neolitsea sericea, Quercus acuta, Hedera rhombea, Fatsia japonica, Camellia japonica, Eurya japonica, Thea sinensis, Acorus gramineus, Pseudosasa japonica and Sasa borealis, and the deciduous subtropical trees such as Carpinus coreana, Aphanth aspera, Orixa japonica, and Lygodium japonicum And it is worth special mention to be discovered Selaginella rossii, Corylopsis coreana, Stewartia koreana, Asarum sieboldii, Viola orientals, and Drosera royundifolia. The Specific plants which is categorized to the Degree by the Ministry of Environment recorded as 45 taxa; 1 species each for the Degree II, IV and V, 5 species for the Degree III, and 37 species for the Degree I.

• Journal of the Korean Applied Science and Technology
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• v.8 no.1
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• pp.35-43
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• 1991

Dried seeds of Camellia japonica and Thea sinensis were investigated to determine the nature of their antioxidative activity. Activity was measured by the induction period in the coupled oxidation of a substrate lard and extracts or isolates to be tested. 70% methanol and dichloromethane extracts were found to be antioxidative abilities. Their unsaponifiables revealed weak antioxidative activity, although hexane extracts did not show antioxidative effect on lard. Column chromatography for dlchloromethane extracts gave 4 fractions(only 2 fractions were potent). HPLC was used in isolating potent antioxidative components from the column fractions and the precolumn-passed methanol extracts. They were separated into 7 and 8 components, respectively. The column fractions obtained from both seeds comprised trans-p-coumaric acid. trans-p-ferulic acid and an unknown component with minor components such as chlorogenic acid and catechin. On the other hand, the most prominent components in the methanol extracts were an unidentified component. trans-pcoumaric acid, trans-p-ferulic acid, catechin and chlorogenic acid. The unknown compound isolated from the column fractions and methanol extracts was identified as epicatechin by $^1H-and\;^{13}C-NMR$. The antioxidative activities of these components were epicatechin > catechin > chlorogenic acid > trans-p-ferulic acid > trans-p-coumaric acid.

• Korean Journal of Plant Resources
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• v.21 no.1
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• pp.12-18
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• 2008

Antioxidative activity and inhibitory activity of angiotensin I converting enzyme(ACE), aminopeptidase N(APN) and $\alpha$-amylase were investigated in the methanol extracts from 25 fern plants native to Jeju Island, in order to screen the plant species containing bioactive materials for functional foods or medicines. The antioxidative activity was higher in Cytomium fortunei(41.9%) and Rumohra standishii(34.1%) than in leaves of Thea sinensis(30.9%), a small tree for antioxidative beverage. Inhibitory activities of ACE and APN were relatively high in Cytomium fortunei as 26.7% and 28.2% respectively. $\alpha$-Amylase inhibitory activity was higher than 50% in 10 species. Particularly, Cytomium fortunei(87.4%) and Dryopteris erythrosora(71.6%) showed the inhibitory activities higher than those of other form plants. Of 25 fern plants investigated here, Cytomium fortunei showed not only the highest antioxidative activity but also the highest inhibitory activity of ACE, APN and $\alpha$-amylase. It suggests that Cytomium fortunei could be potentially used as a resource of bioactive materials for fuctional foods or medicines.

• Korean journal of applied entomology
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• v.41 no.4
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• pp.299-303
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• 2002

Pratylenchus loosi Loof, 1960 is recorded for the first time from Korea. The nematode was isolated from the roots of tea (Thea sinensis L.) and soil around the roots from Yeongam-gun, Jeo-llanam-do and Namjeju-gun, Jeju-do, Korea. This species has two lip annuli. The female is 433-646 $\mu\textrm{m}$ long: a＝ 29.1-37.5, b ＝ 5.1-6.4, c ＝ 15.0-21.3, vulva(%) ＝ 73.0-85.4. The male body length is 408-512$\mu\textrm{m}$ long: a＝36.1-40.0, b＝4.8-6.7, c ＝17.0-19.0, and spicule ＝ 14.1-18.0 $\mu\textrm{m}$. The stylet is 11.6-18.0 $\mu\textrm{m}$ long and the number of incisures is four. The shape of spermatheca filled with sperm is broadly rounded, oval and quadrangular The shape of tail is narrowly round to subacute.

• Food Science and Preservation
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• v.11 no.2
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• pp.207-213
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• 2004

Methanol extracts were prepared from 32 medicinal herbs of the extracts were tested their microbial inhibition activities against food borne pathogens and/or food poisoning microorganisms, food-related bacteria and yeast. Methanol extracts of Cinnamomum cassia, Paeonia suffruticosa, Alnus japonica, Eugenio caryophyllata and Illicium verum exhibited antimicrobial activity for the microorganisms tested, except lactic acid bacteria and yeast. Minimum inhibitory concentrations(MIC) were about 5 mg/mL for Bacillus subtilis, Staphylococcus epidermidis and Pseudomonas aeruginosa. Cell growth of lactic acid bacteria was inhibitied, but greatly on Leuconostoc mesenteroides. The phenolic compound contents were 10.98 mg/g, 10.31 mg/g, 8.55 mg/g and 6.69 mg/g in Thea sinensis, Eugenia caryophyllata, Alnus japonica and Artenisia capillaris, respectively. Antimicrobial activity appeared to be related to phenol compound content in medicinal herbs. The methanol extracts of medicinal herbs could be suitable for the development of a food preservative.

• Korean Journal of Medicinal Crop Science
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• v.4 no.3
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• pp.247-254
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• 1996

The effect of shading on mineral elements, free amino acids, and fatty acids content of tea shoot was examined under different shading periods. The results are summarized as follows. The con­tent of $NH_4^+$, $K^+$. $Mg^{++}$, $PO_4^{--}, was increased, but that of $Na^+$, $Ca^{++}$, $F^-$, $Cl^-$, $No_3^-$, $So_4^{--}$ was de­creased with the passage of shade treatment periods. The content of Total- Nitrogen of tea shoot was the highest in the 55% 10days+95% 5days shading as 6.07%. The content to Total Free Amino Acid was hagher ranged from 20mg/100g to 80mg/100g in shading treatment compered with the un shading treat­ment. The content of Theanine was the highest in 55% 10days+95% 5days shading as 1834mg/100g,while that of unshading was the lowest as 1247mg/100g. The content of Theanine was decreased with the passage of shading periods. The content of Fatty Acid was the highest in the 55% 15days+95% 15days shading as 3164mg/100g, while the unshading treatment had the lowest in the 55% 5days+95% 5days as 2435mg/100g.

• Journal of Korean Society of Forest Science
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• v.66 no.1
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• pp.54-63
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• 1984

In order to compare the variation of Korean native Yea plants, the botanical specimens of the two introduced varieties and the wild varieties collected from 49 places were made. The leaf and flower types from them were investigated. 1) About 78% of the wild tea plants were existed in bamboo thicket or in forest, and 95% were grown in the southward inclined place. 2) The wild tea plants were distributed in the region from $34^{\circ}27^{\prime}$ north to $35^{\circ}43^{\prime}$ north of latitude, but most of them were grown in the region of 35 degree north of latitude. 3) The leaf types of wild tea plants were elliptical and oblong, but onr oval type of leaf was found in the Mand$\ddot{o}$ksa, Kangjin. 4) The Leaf sizes of wild tea plants were $14.74{\pm}0.57{\times}5.72{\pm}0.24cm$ in the large leaf and $12.16{\pm}1.57{\times}3.53{\pm}0.41cm$ in the small leaf. 5) There were two leaf types that one is acuminate, coriaceous, reticulate, dark green, and the other is obtuse, chartaceous, rugose, deep green. 6) There were not much difference between varieties in the flower types, only except the trait of Yongjang. 7) Among the wild tea plants, it was considered that Yongjang-type variety would he a variant or hybrid of bohea-variety (chinese-variety) and Waun-type variety would he a variant or hybrid of macrophylla variety and General-type variety would be a degenerated variant of Waun-type or another hybrid.