• 한국수산과학회지
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• 제48권4호
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• pp.432-438
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• 2015

This study examined the antimicrobial, antioxidant, and tyrosinase inhibitory activities of bioactive compounds extracted from two starfish, Asterina pectinifera and Asterias amurensis, using solvent extraction after $Protamex^{TM}$ hydrolysis. Methanol and acetone fractions collected by stepwise extraction from specimens were subjected to silica gel column chromatography (SGCC) (200 mesh and 400 mesh), followed by high-performance liquid chromatography (HPLC). Two fractions (7:3 and 5:5 chloroform : methanol ratio, v/v) eluted using silica gel column chromatography from the two starfishes showed higher antimicrobial activity against Propionibacterium acnes and dermatophyte fungi (Epidermophyton floccosum, Microsporum audouinii, Trichophyton ferrugineum, Trichophyton mentagrophytes, and Trichophyton rubrum), antioxidant activity ($EDA_{50}$, mg/mL), and tyrosinase inhibitory activity compared to the other fractions. The final fractions obtained from Asterina pectinifera (RT 7.53, 8.93, and 10.48 min) and Asterias amurensis (RT 5.02 min) by SGCC (400 mesh) and HPLC from two SGCC fractions (200 mesh) showed 8.94 and 15.59 mg/mL antioxidant activity ($EDA_{50}$) and 46.89 and 40.19 % tyrosinase inhibitory activity, respectively. Extracts from starfishes are potential cosmetic basic material.

• 한국응용과학기술학회지
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• 제40권1호
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• pp.103-112
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• 2023

본 연구는 우울에 효과가 입증된 국내 자생 식물 9종(배초향, 적송, 강황, 생강, 산미나리씨, 불수감, 황칠, 탱자, 쥐오줌풀) 을 선정하여 각각의 향기 성분 분석결과를 기반으로 9종 정유를 조합하여 항산화(DPPH, ABTS), 세포독성(MTS), 항염(Nitric oxide)실험을 수행하였다. 향기성분 분석결과 조합오일에서 기연구된 우울완화 효과 성분인 DL-Limonene(38.44%), g-Terpinene(8.9%), Estragole(5.18%), a-Pinene(1.73%) 등이 동정되었다. 항산화 활성인 DPPH 라디칼 소거능은 75.4%, ABTS 라디칼 소거능은 74.04%을 보였으며, 세포독성이 확인되지 않은 5uL/ml의 농도에서 NO 생성 저해능은 33.14%로 나타났다. 이를 통하여 국내 블렌딩 정유의 우울증의 개선 또는 예방에 대한 효과를 검증하고 더불어 과학적 효능과 성분 연구가 상호 협력적으로 이루어져 우울증적 증상 개선 유무를 확인 할 수 있는 기초자료로 제공하고자 한다.

• 한국식품저장유통학회지
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• 제13권2호
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• pp.198-203
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• 2006

매실추출물의 항균력을 조사한 결과, $250{\mu}g/mL$ 이상의 농도에서 뚜렷한 항균활성을 보였고, 광역의 병원성 및 부폐성 미생물들에 대하여 항균활성을 나타내었다. 또한, 매실추출물의 항균물질은 넓은 범위의 온도$(40{\sim}120^{\circ}C)$ 및 $pH(3{\sim}11)$에서 안정성을 나타냈다. 따라서 매실추출물의 항균물질은 항균활성이 높고, 항균 spectrum이 광범위할 뿐아니라, 넓은 범위의 온도 및 pH에 안정하여 이상적인 천연 항균제로시의 개발가능성을 제시하였다. 아울러, 매실추출물의 항균작용을 조사한 결과, 매실추출물에는 두 종류 이상의 항균물질이 존재하며 이 물질들의 상승작용으로 항균활성을 나타냄을 확인하였다 매실추출물의 항균활성은 친수성 성분임을 확인하였으며, 항균물질의 주요 작용기작은 세포막 perturbation에 기인한 것으로 사료되었다.

• 생명과학회지
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• 제16권2호
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• pp.253-258
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• 2006

마늘은 전 세계적으로 분포하는 다년생 초본이다. 마늘은 약리적, 경제적으로 중요한 작물이다. 야생종과 재배종의 유전관계를 ISSR 마커로 조사하였다. 또 ISSR 분석으로 이들 종의 유전적 다양도와 집단구조를 실시하였다. 한국의 세 야생 집단은 분리되어 있고 패치 분포를 보이지만 재배종에 비해 높은 유전적 다양성을 유지하고 있었다. ISS5R 마커로 야생종과 재배종의 계통관계는 잘 분리되었다. 비록 한국내 재배종 마늘이 산마늘에서 진화하였 다는 직접적 증거는 없지만 본 연구 결과 그런 가능성은 시사된다. 또한 야생종 산마늘 집단은 생식질 동정과 재배종 마늘의 진화과정에서 유익하게 쓰일 수 있다.

• 식물분류학회지
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• 제47권1호
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• pp.11-22
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• 2017

Prangos fedtschenkoi (Regel et Schmalh.) Korovin (Apiaceae) is an endemic species for mountainous Middle Asia, which is both a rare and useful plant. Organic extractions from this species are being used in pharmaceutics and cosmetology. In recent years, P. fedtschenkoi distribution area has considerably decreased, presumably, resulting from human activities such as agriculture, construction works, overgrazing and collection from wild for pharmaceutic purposes. Six populations were found in Uzbekistan and their genetic divergence and differentiation were studied with 10 inter-simple sequence repeat (ISSR) markers, selected out of 101. Totally 166 amplified ISSR fragments (loci) were revealed, of which 164 were polymorphic. Relatively moderate level of polymorphism was found at population level with polymorphic bands ranging from 27.71% to 47.59%. Mean P = 39.05%, $N_a=1.40$, $N_e=1.25$, S.I. = 0.21, and $H_e=0.14$ were revealed for all loci across six populations. AMOVA showed higher variation among populations (62%) than within them (38%). The Bayesian model determined 5 clusters, or genetic groups. The posteriori distribution of the Theta II estimator detected full model identifying high inbreeding, intensified by low gene flow (Nm = 0.3954). Mantel test confined population 6 as distinct cluster corresponding to geographic remoteness (R = 0.5137, $p{\leq}0.005$). Results were used as the bases for developing conserve measures to restore populations.

• 한국식품저장유통학회지
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• 제14권2호
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• pp.183-187
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• 2007

매실박 메탄을 추출물의 항균력을 조사한 결과, $500{\mu}g/mL$ 이상의 농도에서 뚜렷한 항균활성을 보였고, 광역의 변패성 미생물들에 대하여 항균활성을 나타내었다. 또한, 매실박 메탄올추출물의 항균물질은 높은 온도$(100^{\circ}C)$에서 30분 동안 안정하였으며, 넓은 범위의 pH(3-11)에서 안정성을 나타냈다. 따라서 매실박 메탄올 추출물의 항균물질은 항균활성이 높고, 항균s spectrum이 광범위할 뿐 아니라, 높은 온도 및 넓은 범위의 pH에 안정하여 이상적인 천연 항균제로서의 개발가능성을 제시하였다. 아울러, 매실박 메탄올 추출물의 주요 항균작용은 세포막 perturbation에 기인한 것으로 생각되었다.

• 패션비즈니스
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• 제13권6호
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• pp.45-60
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• 2009

This study examined the efficacy of restraining the increase of the bacteria colony of scalp according to the application of the Ayurvedic base oils and essential oils to scalp and hair. The result is as follows. The experiment illustrated that as the result of doing the paired sample t-test of a treated group and a control group according to constitutional oiling, the efficacy of the statistically significant decrease of the colony manifested in six tested groups except the group of Pitta constitution to which sesame and lavender were applied. Also, in a group that sesame was applied to Vata constitution and a group that coconut and lavender were applied to Pitta constitution, according to the passage of time, the result of being reduced the number of the colony was obtained and it showed that the oiling suitable for each constitution has the efficacy to decrease the colony of scalp. At the same time, regardless of the constitutions, the application of oils also showed the efficacy of restraining the increase of the bacteria colony of scalp. As the result of performing Paired Sample t-test for the subjective evaluation of the subjects in pre and post clinical testing, In the analysis of questionnaire that were obtained before and after the clinical testing, the results of all of the items except the item questioning a degree of inflammation appeared to be significant. That is to say, the subjects answered that a degree of keratin or a pain in scalp, an amount of sebum and a degree of hair damage were decreased after the application of oil more than before it. And in the question of a degree of hair damage, the application of oils were proved as having the efficacy to improve the hair damage.

• 패션비즈니스
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• 제15권2호
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• pp.1-11
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• 2011

The purpose of this study is to investigate the influence of the suitability of the chronic regulatory focus and the advertising message type on the evaluation of the beauty product. In addition, feeling right while and individual is reading an advertisement is measured to check the mechanism making the regulatory fit effect. The experimental design was designed with the mixed design of 2(chronic regulatory focus: promotion / prevention, between group) ${\times}$ 2(advertising message type: promotion /prevention, within a group). 100 female university student subjects in their twenties in Seoul and in the metropolitan area were asked to respond to questionnaires in the study. The reliability analysis, T-test, analysis of variance(ANOVA), and analysis of covariance (ANCOVA) on the collected data were done with SPSS WIN 12.0. The results of the study are as follows. First, the regulatory fit effect that when the individual chronic regulatory fit corresponds to the advertising message focus each other in the advertising of a new beauty product, the evaluation on the product is more positive could be checked. The promotion focus message of shampoo, the promotion focus group showed more positive response than the prevention focus group and as for the prevention focus message, the prevention focus group showed more positive results than the promotion focus group so that the effect of regulatory fit appeared. Second, when the regulatory fit effect appeared in the evaluation on the new beauty product, the fit effect on the individual chronic regulatory focus and on the advertising message focus also appeared in the measurement of feeling right. Hence, feeling right could be checked by using the mechanism of the regulatory fit effect.

• 패션비즈니스
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• 제23권1호
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• pp.116-126
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• 2019

The purpose of this study is to objectify the shape of curvature and average change rate of angular and arched eyebrow makeup. The data for the analysis were the final 336 pieces for the cosmetology students in the whole country. SPSS 20.0 program was used for the collected data, frequency analysis, factor analysis, reliability analysis, and crossover analysis were performed. First, the frequency of the average change rate of eyebrow makeup (angular and arcuate) was analyzed. As a result, the eyebrows suitable for the angled type showed the highest level of 'A-2' and the arched eyebrows had the highest level of 'B-2'. Second, to analyze the important factors of eyebrow makeup, frequency of eyebrows was the most important, followed by the inclination of eyebrows and eyebrow tail. Third, factor analysis and reliability analysis were conducted to examine each dimension of eyebrow makeup perception, and it was derived from three factors including acceptability, aggressiveness, and playability. Fourth, in order to analyze the difference of important factors in the form of the standard eyebrow makeup, the cross - analysis was performed with chi - square test, and it was confirmed that the angular eyebrow makeup showed the most objectivity when the average change rate was 0.29 and the curvature was 0.67. Fifth, arcuate eyebrow makeup showed the most objectivity when the average change rate was 0.57 and curvature was 0.56. The results of this analysis can be used as objective data of aesthetic criteria when designing the eyebrow shape.

• 패션비즈니스
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• 제12권6호
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• pp.1-10
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• 2008

Public interest in healthy hairs gets growing as damaged hairs are seen more frequently with the generalization of heat permanent waves. For this study, experiments have been conducted to understand the influences on the changes in physical and morphological features of wave forms and damaged hairs, by collecting virgin hairs from the women in their mid-20's, who had not experienced chemical applications, and by dividing the applications of heat perm hair treatments, PPT(for pre-treatment) and LPP(for post-treatment), into the pre-treatment, the post-treatment, the pre & post-treatment, and the non-treatment. For the wave formations, curl waves were investigated by the bare eyes using the pictures taken by a digital camera. For the comparison of physical features, the experiments of tensile strength and elongation were done and their mean values were found. For the observations of morphological features, the pictures were taken by SEM for comparison. As for the findings, regarding the curl wave shapes of hairs, the most even and elastic S curl was formed in the case of non-treatment. In the physical features, both of the tensile strength and elongation showed a decreasing tendency in line with the hair damage levels, and the case of the pre & post-treatment indicated the tendency most similar to the control group. In the morphological features of the cuticle, observed with an SEM, the pre-treatment showed the higher possibility of reducing the cuticle damages than the post-treatment did. LPP was found to play the role of protective membrane for the post-treatment, and the pre & post-treatment turned out to reduce most effectively the cuticle damages.