• Journal of Food Hygiene and Safety
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• v.27 no.1
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• pp.37-41
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• 2012

The current standard for testing tetrodotoxin (TTX) in foodstuffs is the mouse bioassay (MBA) in Korea as in many other countries. However, this test suffers from potential ethical concerns over the use of live animals. In addition, the mouse bioassay does not test for a specific toxin thus a sample resulting in mouse incapacitation would need further confirmatory testing to determine the exact source toxin (e.g., TTX, STX, brevotoxin, etc.). Furthermore, though the time of death is proportional to toxicity in this assay, the dynamic range for this proportional relationship is small thus many samples must be diluted and new mice be injected to yield a result that falls within the quantitative dynamic range. Therefore, in recent years, there have been many efforts in this field to develop alternative assays. High performance liquid chromatography (HPLC) coupled with mass spectrometry (MS) has been emerged as one of the most promising options. A LC-MS-MS method involves solid-phase extraction (SPE) and followed by analysis using an electrospray in the positive ionization mode and multiple reactions monitoring (MRM). To adopt LC-MS-MS method as alternative standard for testing TTX, we performed a validation study for the quantification of TTX in puffer fish. This LC-MS-MS method showed good sensitivity as limits of detection (LOD) of $0.03{\sim}0.08{\mu}g/g$ and limits of quantification (LOQ) of $0.10{\sim}0.25{\mu}g/g$. The linearity ($r^2$) of tetrodotoxin were 0.9986~0.9997, the recovery were 80.9~103.0% and the relative standard deviations (RSD) were 4.3~13.0%. The correlation coefficient between the mouse bioassay and LC/MS/MS method was higher than 0.95.

• Journal of the Korea Academia-Industrial cooperation Society
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• v.21 no.6
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• pp.18-27
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• 2020

This study compared and analyzed the results of proficiency testing between testers based on the international standard ISO/IEC 17043 proficiency testing requirements using ISO/IEC 25023 software quality characteristics evaluation. Application of the test was submitted to the KASTO as a software test in the electric test field defined by KOLAS. Among ISO/IEC 25023, the evaluation items for proficiency testing between testers were selected as functional completeness and time behavior. The comparison test between testers was carried out on May 28, 2019 at the accredited LG Electronics SW Laboratory in Seoul and attended by KOLAS assessors. The test evaluation method was measured once per tester with functional completeness, and measurement was repeated 10 times per tester according to time behavior. As a result, in the case of functional completeness, all 12 test steps were passed, and the satisfaction was evaluated. Time behavior was repeated 10 times, and the mean was 9.45 ± 0.01 seconds for testers 1 and 3 and 9.52 ± 0.0 seconds for tester 2, but there was no statistically significant difference. In conclusion, the proficiency of the testers was equal, and the reliability of the test results of the test institute was confirmed.

• Journal of the Korean Society for Nondestructive Testing
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• v.20 no.1
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• pp.10-17
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• 2000

In boiler high-temperature pipelines such as main steam pipe, header and steam drum in fossil power plants, conventional measurement techniques(replica method, electric resistance method, and hardness test method) for measuring creep damage have such disadvantages as complex preparation and measurement procedures, too many control parameters. And also, these techniques have low practicality and applied only to component surfaces with good accessibility. It needs to apply a reliable and quantitative ultrasonic nondestructive evaluation method that can be replaced for these equipment. In this study, both artificial creep degradation test using life prediction formula and frequency analysis by ultrasonic tests for crept specimens were carried out for the purpose of nondestructive evaluation for creep damage. As a result of ultrasonic tests for crept specimens, we conformed that the high frequency side spectra decrease and central frequency components shift to low frequency band, and also their bandwidth decreases as increasing creep damage in backwall echos.

• Proceedings of the Korea Concrete Institute Conference
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• 1999.04a
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• pp.697-702
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• 1999

Among several non-destructive testing methods, ultrasonic pulse velocity method has been widely used for the evaluation of concrete strength. However, this method might not provide accurate estimated results since factors influencing the relationship between strength and wave velocity is not considered. In this study, the evaluation methods of concrete strength using compression wave velocities measured by either ultrasonic pulse velocity method or impact-resonance method are proposed. A basic equation is obtained by the linear regression with velocity vs. strength data at a specific age and then, ageing factor is employed in the equation to consider the difference of the increasing rate between wave velocity and strength. Strengths predicted by the proposed equation agree well with test results.

• Journal of the Korean Society for Nondestructive Testing
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• v.7 no.1
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• pp.42-50
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• 1987

Most of significant defects in the pressure boundaries of nuclear power plant we re dispositioned to be monitored periodically every inservice inspection. Due to the difficulty of the defect sizing during operation, it is necessary to develope the continuous flaw monitoring techniques. The Tip Diffraction method, specifically speaking, spot seems to be suitable for flaw monitoring. The optimum conditions of selecting the transducer were 3.5 MHz and 45-57 degree according to compatibility with the defect height. The effective calculation of the defect height was to assume the fact that the incident beam is parallel. This method would be supplemented to ASME method about the defect characterization for the surface flaw.

• The Transactions of the Korea Information Processing Society
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• v.6 no.6
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• pp.1598-1608
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• 1999

Performance testing for ATM network deals with evaluation of maximal throughput of network by measuring and analyzing of various performance parameters. However, because of the absence of the methodology and framework for performance testing, the results of performance measurements for same implementation under test were much relied on tester. In this paper, the concept of ATM performance testing is described in both cell and frame level and a framework of ATM performance testing is suggested by describing of testing environment for performance measurement and requirements of performance evaluation. For this, we define performance measuring method and parameters for each case.

• Proceedings of the KSR Conference
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• 2007.11a
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• pp.1940-1945
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• 2007

The wheelsets are one of most important component: damages in wheel tread and press fitted axle are a significant cost for railway industry. Since failure in railway wheelset can cause a disaster, regular inspection of defects in wheels and axles are mandatory. Ultrasonic testing, acoustic emission and eddy current testing method and so on regularly check railway wheelset in service. However, it is difficult to use this method because of its high viscosity and because its sensitivity is affected by temperature. Also, due to noise echoes it is difficult to detect defects initiation clearly with ultrasonic testing. It is necessary to develop a non-destructive technique that is superior to conventional NDT techniques in order to ensure the safety of railway wheelset. In the present paper, the new NDT technique is applied to the detection of surface defects for railway wheelset. To detect the defects for railway wheelset, the sensor for defect detection is optimized and the tests are carried out with respect to surface and internal defects each other. The results show that the surface crack depth of 1.5 mm in press fitted axle and internal crack in wheel could be detected by using the new method. The ICFPD method is useful to detect the defect that initiated in the tread of railway wheelset.

• Journal of the Korean Society for Nondestructive Testing
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• v.35 no.1
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• pp.61-67
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• 2015

Recently, the initiation of outside diameter stress corrosion cracking (ODSCC) at the tube support plate region of domestic steam generators (SG) with Alloy600 HTMA tubes has been increasing. As a result, SGs with Alloy600 HTMA tubes must be replaced early or are scheduled to be replaced prior to their designed lifetime. ODSCC is one of the biggest threats to the integrity of SG tubes. Therefore, the accurate evaluation of tube integrity to determine ODSCC is needed. Eddy current testing (ECT) is conducted periodically, and its results could be input as parameters for evaluating the integrity of SG tubes. The reliability of an ECT inspection system depends on the performance of the inspection technique and abilty of the analyst. The detection probability and ECT sizing error of degradation are considered to be the performance indices of a nondestructive evaluation (NDE) system. This paper introduces an optimized evaluation method for ECT, as well as the sizing error, including the analyst performance. This study was based on the results of a round robin program in which 10 inspection analysts from 5 different companies participated. The analysis of ECT sizing results was performed using a linear regression model relating the true defect size data to the measured ECT size data.

• Journal of Pharmaceutical Investigation
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• v.30 no.3
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• pp.223-228
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• 2000

Testing the volume of injection in container is to ensure that the nominal content of a container can be administered to the patient. The method to test the volume of injection in container is described in European Pharmacopeia (EP) and United States Pharmacopeia (USP). However there is no method to test the volume of injection in Korean Pharmacopeia (KP). So we surveyed the method practiced by 53 pharmaceutical companies in Korea by a questionnaire and tested commercially available injections by discharged volume. As the result, we agreed with the International Conference on Harmonization (ICH) test method for discharged volume. It was suggested that the ICH test method for discharged volume of injection in container would be adopted.

• Coupled systems mechanics
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• v.7 no.3
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• pp.297-319
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• 2018

In this paper, the vibro-acoustic behaviors of vibrational cylindrical shells are investigated by using structural intensity approach. The reducing interior noise method for vibrating cylindrical shells is proposed by altering and redistributing the structural intensity through changing the damping property of the structure. The concept of proposed novel method is based on the properties of structural intensity distribution on cylindrical shells under different load and damping conditions, which can reflects power flow in the structures. In the study, the modal formulas of structural intensity are developed for the steady state vibration of cylindrical shell structures. The detailed formulas of structural intensity are derived by substituting modal quantities, in which the effect of main parameters such as weight coefficients and distribution functions on structure intensity are analyzed and discussed. Numerical simulations are first carried out based on the structural intensity analytical solutions of modal formulas. Through simulating the coupling vibration and acoustical radiation problems of cylindrical shell, the relationship between vibro-acoustic and structural intensity distribution is derived. We find that for cylindrical shell, by properly arranging damping conditions, the structural intensity can be efficiently changed and further the noise property can be improved. The proposed methodology has important implications and potential applications in the vibration and noise control of fuselage structure.