• Title/Summary/Keyword: Temperature stress

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Hematological Characteristics of Olive Flounder (Paralichthys olivaceus) in Culture Farm Influenced by Heated Effluent Water from a Power Plant in Summer (여름철 온배수 수역에 인접한 양식장 넙치(Paralichthys olivaceus)의 혈액 성상)

  • Chang, Young-Jin;Hur, Jun-Wook;Chin, Pyung
    • Journal of Aquaculture
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    • v.15 no.4
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    • pp.267-273
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    • 2002
  • Effect of heated water from a power plant in summer on hematological characteristics of flounder (Paralichthys olivaceus) were investigated. Hematocrit level fluctuated between 15.7 and 16.3% in control farm, but it significantly increased to 17.7% in the high-WT farm. The corresponding values for hemoglobin concentration were 9.9~18.0 $g/㎗$ for the control farm and 6.2~19.4 $g/㎗$ for the exposed farm. The plasma cortisol remained between 0.6 and 2.8 ng/$ml$ in the former but significantly decreased 317.1 to 3.3 ng/$ml$ in the later. The plasma glucose also decreased from 36.5~46.0 to 23.5~36.5 mg/$dl$. Plasma osmolality decreased from 551.5~597.0 to 391.0~466.5 mOsm/kg.

Polymorphism of the Promoter Region of Hsp70 Gene and Its Relationship with the Expression of HSP70mRNA, HSF1mRNA, Bcl-2mrna and Bax-AMrna in Lymphocytes in Peripheral Blood of Heat Shocked Dairy Cows

  • Cai, Yafei;Liu, Qinghua;Xing, Guangdong;Zhou, Lei;Yang, Yuanyuan;Zhang, Lijun;Li, Jing;Wang, Genlin
    • Asian-Australasian Journal of Animal Sciences
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    • v.18 no.5
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    • pp.734-740
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    • 2005
  • The blood samples were collected from dairy cows at the same milking stage. The single-strand conformation polymorphism (PCR-SSCP) method was used to analyze for polymorphism at the 5'flanking region of the hsp70 gene. The mRNA expression levels of HSP70, HSF1, Bcl-2 and Bax-$\alpha$ at different daily-mean-temperature were analyzed by relative quantitative RTPCR. The DNA content, cell phase and the ratio of apoptosis of lymphocytes in peripheral blood of dairy cattle at different daily-meantemperature were determined by FCM. The PCR-SSCP products of primer pair 1 showed polymorphisms and could be divided into four genotypes: aa, ab, ac, cc, with the cis-acting element (CCAAT box) included. Mutations in the hsp70 5'flanking region (468-752 bp) had different effects on mRNA expression of HSP70, HSF1, Bcl-2 and Bax-$\alpha$. The ac genotypic cows showed higher expressions of HSP70mRNA, HSF1mRNA and Bcl-2mRNA/Bax-$\alpha$mRNA and lower ratio of apoptosis. These mutation sites can be used as molecular genetic markers to assist selection for anti-heat stress cows.

Thermomechanical Analysis of Functionally Gradient $Al-SiC_p$ Composite for Electronic Packaging (전자패키지용 경사조성 $Al-SiC_p$복합재료의 열.기계적 변형특성 해석)

  • 송대현;최낙봉;김애정;조경목;박익민
    • Composites Research
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    • v.13 no.6
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    • pp.23-29
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    • 2000
  • The internal residual stresses within the multilayered structure with sharp interface induced by the difference in thermal expansion coefficient between the materials of adjacent layers often provide the source of failure such as delamination of interfaces etc. Recent development of the multilayered structure with functionally graded interface would be the solution to prevent this kind of failure. However a systematic thermo-mechanical analysis is needed for the customized structural design of multilayered structure. In this study, theoretical model for the thermo-mechanical analysis is developed for multilayered structures of the $Al-SiC_p$ functionally graded composite for electronic packaging. The evolution of curvature and internal stresses in response to temperature variations is presented for the different combinations of geometry. The resultant analytical solutions are used for the optimal design of the multilayered structures with functionally graded interface as well as with sharp interface.

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Evaluation of Stress Reduction of Continuous Welded Rail of Sliding Slab Track from Track-Bridge Interaction Analysis (궤도-교량 상호작용 해석에 의한 슬라이딩 슬래브 궤도의 장대레일 응력 저감 효과 분석)

  • Lee, Kyoung Chan;Jang, Seung Yup;Jung, Dong-Ki;Byun, Hyung-Kyoon
    • KSCE Journal of Civil and Environmental Engineering Research
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    • v.35 no.5
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    • pp.1179-1189
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    • 2015
  • Continuous welded rail on bridge structure experiences typically a large amount of additional longitudinal axial forces due to longitudinal track-bridge interaction under temperature and traction/braking load effect. In order to reduce the additional axial forces, special type of fastener, such as ZLR and RLR or rail expansion joint should be applied. Sliding slab track system is known to reduce the effect of track-bridge interaction by the application of a sliding layer between slab track and bridge structure. This study presents track-bridge interaction analysis results of the sliding slab track and compares them with conventional fixed slab track on bridges. The result shows that the sliding slab track can significantly reduce the additional axil forces of the continuously welded rail, and the difference is more significant for long and continuous span bridge.

Durability Evaluation of Thermal Barrier Coating (TBC) According to Growth of Thermally Grown Oxide (TGO) (TGO 성장을 고려한 열차폐코팅의 내구성평가)

  • Song, Hyun Woo;Moon, Byung Woo;Choi, Jae Gu;Choi, Won Suk;Song, Dongju;Koo, Jae-Mean;Seok, Chang-Sung
    • Transactions of the Korean Society of Mechanical Engineers A
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    • v.38 no.12
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    • pp.1431-1434
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    • 2014
  • The thermal barrier coating (TBC) applied to a gas turbine can be damaged by repeated thermal fatigue during operation, so an evaluation of its durability is needed. Thermally grown oxide (TGO) is generated inside the TBC in a high-temperature environment. The growth of TGO is known to be the main cause of damage to the TBC. Therefore, the durability of TBC should be evaluated according to the growth of TGO. In this research, Kim et al.'s work on the growth of TGO with aging was used as a basis for finite element analysis. The relationship between stress and aging was derived from the finite element analysis results. The durability of the TBC with aging was evaluated through a comparison between the results of the finite element analysis and a bond strength test.

Growth Dynamics of Zostera marina Transplants in the Nakdong Estuary Related to Environmental Changes (낙동강 하구에 이식된 잘피(Zostera marina)의 환경변화에 따른 성장특성)

  • Park, Jung-Im;Lee, Kun-Seop;Son, Min-Ho
    • Korean Journal of Fisheries and Aquatic Sciences
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    • v.44 no.5
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    • pp.533-542
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    • 2011
  • Numerous seagrass habitat restoration projects have been attempted recently due to the remarkable decline in seagrass coverage. Seagrass transplants tend to adapt to a new environment after experiencing transplanting stress during the early stages of transplantation. Once acclimated, the transplants grow into healthy seagrass beds via vegetative propagation. The establishment and growth dynamics of transplanted seagrasses in bays and coasts are widely reported, but few studies have been conducted on estuaries in Korea. We transplanted Zostera marina in November 2007 and November 2008 in the Nakdong estuary using the staple method, and monitored the survival, adaptation, and growth dynamics of the transplants as well as environmental factors every month for 1 year. Both transplants adapted well to the new environment without initial losses and showed rapid productivity during early summer. However, density of transplants increased 320% in 1 year from the previous year's transplants but that decreased to 59% during the following year. This significant reduction in density in the second year may have been caused by exposure to low salinity (10 psu) for 3 weeks during the unusually long monsoon season. While the survival and growth dynamics of seagrass transplants planted in bays and coasts are mainly controlled by underwater photon flux density and water temperature, salinity was the critical factor for those planted in Nakdong estuary.

Genomic Organization and Isoform-Dependent Expression Patterns of Wap65 genes in Various Tissues during Immune Challenges in the Mud Loach Misgurnus mizolepis

  • Kim, Yi Kyung;Cho, Young Sun;Lee, Sang Yoon;Nam, Yoon Kwon
    • Fisheries and Aquatic Sciences
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    • v.17 no.4
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    • pp.471-478
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    • 2014
  • Genomic organization, including the structural characteristics of 5'-flanking regions of two 65-kDa protein (WAP65) isoform genes associated with warm temperature acclimation, were characterized and their transcriptional responses to immune challenges were examined in the intestine, kidney and spleen of the mud loach (Misgurnus mizolepis; Cypriniformes). Both mud loach Wap65 isoform genes displayed a 10-exon structure that is common to most teleostean Wap65 genes. The two mud loach Wap65 isoforms were predicted to possess various stress- and immune-related transcription factor binding sites in their regulatory regions; however, the predicted motif profiles differed between the two isoforms, and the inflammation-related transcription factor binding motifs, such as NF-${\kappa}B$ and CREBP sites, were more highlighted in the Wap65-2 isoform than the Wap65-1 isoform. The results of qRT-PCR indicated that experimental immune challenges using Edwardsiella tarda, lipopolysaccharide or polyI:C induced the Wap65-2 isoform more than Wap65-1 isoform, although modulation patterns in response to these challenges were tissue- and stimulant-dependent. This study confirms that functional diversification between the two mud loach Wap65 isoforms (i.e., closer involvement of Wap65-2 in the acute phase of inflammation and innate immunity) occurs at the mRNA level in multiple tissues, and suggests that such differential modulation patterns between the two isoforms are related to the different transcription factor binding profiles in their regulatory regions.

Toxic effects of ammonia exposure on growth performance, hematological parameters, and plasma components in rockfish, Sebastes schlegelii, during thermal stress

  • Shin, Ki Won;Kim, Shin-Hu;Kim, Jun-Hwan;Hwang, Seong Don;Kang, Ju-Chan
    • Fisheries and Aquatic Sciences
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    • v.19 no.10
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    • pp.44.1-44.8
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    • 2016
  • Rockfish, Sebastes schlegelii (mean length $14.53{\pm}1.14cm$ and mean weight $38.36{\pm}3.45g$), were exposed for 4 weeks with the different levels of ammonia in the concentrations of 0, 0.1, 0.5, and 1.0 mg/L at 19 and $24^{\circ}C$. The indicators of growth performance such as daily length gain, daily weight gain, condition factor, and hematosomatic index were significantly reduced by the ammonia exposure and high temperature. The ammonia exposure induced a significant decrease in hematological parameters, such as red blood cell (RBC) count, white blood cell (WBC) count, hemoglobin (Hb), and hematocrit (Ht), whose trend was more remarkable at $24^{\circ}C$. Mean corpuscular volume (MCV), mean corpuscular hemoglobin (MCH), and mean corpuscular hemoglobin concentration (MCHC) were also notably decreased by the ammonia exposure. Blood ammonia concentration was considerably increased by the ammonia concentration exposure. In the serum components, the glucose, glutamic oxalate transaminase (GOT), and glutamic pyruvate transaminase (GPT) were substantially increased by the ammonia exposure, whereas total protein was significantly decreased. But, the calcium and magnesium were not considerably changed.

Grain Refinement and Mechanical Properties of AM60 Mg Alloy by $CaCN_2$ Addition (Ca$CN_2$ 첨가에 의한 AM60 마그네슘 합금의 결정립 미세화 및 기계적 성질)

  • Eom, Jeong-Pil;Jeong, Seong-Kyu;Lim, Su-Geun;Shin, Hee-Taek;Jeong, Deuk-Soo
    • Journal of Korea Foundry Society
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    • v.18 no.4
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    • pp.383-388
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    • 1998
  • Effects of $CaCN_2$ addition on the grain refinement in the AM60 magnesium ingots were investigated. The effects of the $CaCN_2$ are estimated with different inoculation temperatures, inoculation contents, and holding time to find out the optimum condition. AM60 alloy was melted in the low carbon steel crucible by cylindrical electric furnace under an argon atmosphere. The melting and casting apparatus is specially designed for magnesium alloys. The grain size of AM60 magnesium alloy decreased significantly with an increase in $CaCN_2$ content and, at 0.8 wt% $CaCN_2$ or more, grain size becomes constant at about $85 {\mu}m$. The optimum condition was obtained in the 0.8 wt% $CaCN_2$ for holding molten metal of 30 min. at the temperature of $710^{\circ}C$. The tensile properties of AM60 magnesium alloys were improved due to grain refinement by addition of $CaCN_2$. In the optimum condition, the yield strength, tensile strength and elongation were ${\sigma}_{0.2}=107 MPa$, ${\sigma}_{T.S}=234 MPa$ and e=14.2%. The variation of stress with strain obeyed the relationship of the ${\sigma}=K{\varepsilon}^n$. The strain-hardening exponent, n and strength coefficient, K obtained in the 0.8 wt% $CaCN_2$ added AM 60 magnesium alloy were n=0.21 and K=390 MPa.

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THE EFFECTS OF HEAT TREATMENT ON MECHANICAL PROPERTIES AND METAL RELEASE FROM HEAT-TREATED ORTHODONTIC ARCHWIRES (열처리한 교정용 호선의 기계적 성질과 금속유리에 대한 연구)

  • Choi, Chul Min;Rhee, Byung Tae
    • The korean journal of orthodontics
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    • v.20 no.2
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    • pp.381-390
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    • 1990
  • The purpose of this study was to evaluate the effects of heat treatment on mechanical properties in $0.016^{{\prime}{\prime}}{\times}0.022^{{\prime}{\prime}}$ blue Elgiloy wires and to measure the amounts of nickel and chromium released from the wires in artificial saliva. Ultimate tensile strength, yield strength and elongation were measured with universal testing machine (Instron). and the amounts of nickel and chromium released from the sample were measured with atomic absorption spectrophotometer after one week immersion in the artificial saliva. Ultimate tensile strength and yield strength were progressively increased below $1000^{\circ}F$, but elongation was decreased. And the results were reversed above $1100^{\circ}F$. After heat treatment of the sample for 9 minutes at $1000^{\circ}F$, the maximum tensile strength and yield strength were $213.6kg/mm^2$, $140.1kg/mm^2$, respectively. Both tensile strength and yield strength were decreased progressively above $1100^{\circ}F$. Elongation was appeared the minimum value (8.6%) after heat treatment for 9 minutes and 12 minutes at $1000^{\circ}F$. There was a pronounced increase in nickel and chromium liberation with increasing time and temperature. The maximum amounts of nickel and chromium released form the sample were $4.947{\mu}m/cm^2$, $3.088{\mu}g/cm^2$, respectively after heat treatment for 12 minutes at $1300^{\circ}F$. Heat treatment is applied to orthodontic wires, especially cobalt-chromium alloys, for the purpose of stress-relievning and hardening. When the heat-treatment of orthodontic wires, the heating procedure should be well controlled in order to have no effect on corrosion resistance and mechanical properties.

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