• Journal of Microbiology
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• 제43권4호
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• pp.345-353
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• 2005

The complex ecosystem of intestinal micro flora is estimated to harbor approximately 400 different microbial species, mostly bacteria. However, studies on bacterial colonization have mostly been based on culturing methods, which only detect a small fraction of the whole microbiotic ecosystem of the gut. To clarify the initial acquisition and subsequent colonization of bacteria in an infant within the few days after birth, phylogenetic analysis was performed using 16S rDNA sequences from the DNA iso-lated from feces on the 1st, 3rd, and 6th day. 16S rDNA libraries were constructed with the amplicons of PCR conditions at 30 cycles and $50^{\circ}C$ annealing temperature. Nine independent libraries were produced by the application of three sets of primers (set A, set B, and set C) combined with three fecal samples for day 1, day 3, and day 6 of life. Approximately 220 clones ($76.7\%$) of all 325 isolated clones were characterized as known species, while other 105 clones ($32.3\%$) were characterized as unknown species. The library clone with set A universal primers amplifying 350 bp displayed increased diversity by days. Thus, set A primers were better suited for this type of molecular ecological analysis. On the first day of the life of the infant, Enterobacter, Lactococcus lactis, Leuconostoc citreum, and Streptococcus mitis were present. The largest taxonomic group was L. lactis. On the third day of the life of the infant, Enterobacter, Enterococcus faecalis, Escherichia coli, S. mitis, and Streptococcus salivarius were present. On the sixth day of the life of the infant, Citrobacter, Clostridium difficile, Enterobacter sp., Enterobacter cloacae, and E. coli were present. The largest taxonomic group was E. coli. These results showed that microbiotic diversity changes very rapidly in the few days after birth, and the acquisition of unculturable bacteria expanded rapidly after the third day.

• Applied Chemistry for Engineering
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• 제9권5호
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• pp.742-748
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• 1998

Experiments have been conducted to investigate synergistic effects and mechanisms of the Alaskan subbituminous coal/polypropylene (PP). Coliquefaction of coal/PP gave the synergistic effect in yields by 17% compared to independent liquefactions of coal or PP at $430^{\circ}C$. To analyse coliquefaction mechanisms, the second and zeroth order kinetic models were developed for coal and PP conversions respectively. When the models were simulated to fit coliquefaction results, those represented results successfully with the correlation coefficient of 0.99. The amount of tetralin needed to liquefy unit mass of coa 1(${\beta}$) was also calculated using the developed model. According to the calculated results, $\beta$ increased from 0.4 to 1.0 as the coliquefaction temperature increased from $410^{\circ}C$ to $470^{\circ}C$. This indicated that tetralin lowered the molecular weight of oil produced, and this phenomenon was recognized by the GPC analyses. Furthermore, it was found that tetralin prohibited the liquefaction of PP when coal was not added. On the other hand, tetralin was believed to act as a hydrogen-donor solvent to coal rather than prohibit PP liquefaction during coliquefaction. Therefore, the liquefaction rate of PP increases and synergistic effects in oil yields are mainly due to the increase in PP liquefaction during coal/PP coliquefaction.

• Journal of Food Hygiene and Safety
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• 제27권4호
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• pp.479-486
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• 2012

This study was conducted to demonstrate quality characteristics of ginger by making aged ginger( AG) with two methods, the frist method was that ginger was aged at constant temperature and humidity chamber for the duration of 10, 20 and 30 days, and the other method was drying the ginger just after steaming it for 3 hours. As the age was being processed, the volume of ginger's appearance decreased rapidly and its color seemed to be darker because of the decrease in moisture. In the case of general components, the content of crude ash was depended on aged periods while the content of crude fat was independent with aged periods, and according to the content of crude protein, there was not any significant differences. The main valuable ingredient which is 6-gingerol showed the decreasing trend as it was exposed to heat with more time, and 6-shogaol which is also one of the main valuable ingredients showed high content at T-II(AG-10days). Free sugar and free amino acid of AG decreased as aged period goes by, and this study found that there were lots of essential amino acid (threonine, glutamic acid, alanine, valine, leucine and tyrosine) in ginger. The amount of unsaturated fatty acid of AG was significantly higher than the amount of saturated fatty acid of AG with the approximate ratio of 60:40, and the amount of free fatty acid of AG did not seem any big differences between AG and none AG. Considering both valuable ingredients and nutritive components, T-I (steamed ginger, SG) and T-II which was aged for 10 days were evaluated excellently.

• Korean Journal of Food Science and Technology
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• 제32권2호
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• pp.312-316
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• 2000

Crude xylooligosaccharides(XO) were separated with a cellulose membrane (10,000 molecular weight cut-off). Flux variation was measured at transmembrane pressures of 5, 10, 20 and 30 psi, while flow rates were maintained at 100, 200, 300 and 400 ml/min and temperatures at 10, 20, 23-25 and $30^{\circ}C$. Optimal conditions were found at the transmembrane pressure of 20 psi, the flow rate of 300 ml/min and the temperature of $23-25^{\circ}C$, where the flux was decreasing with time by 62% after 200 min. Total sugar, total solid and ash contents were rather independent of ultrafiltration but protein was removed up to 55% from crude XO. Especially, the use of ultrafiltration was very effective for decolorization (84%) of crude XO. These results suggest that ultrafiltration is a useful method for the primary purification of XO.

• Journal of Environmental Health Sciences
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• 제47권5호
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• pp.472-478
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• 2021

Background: The Legionella case detection and notification rate have increased in public artificial water environments where people visit, including large buildings, public baths, and hospitals. Objectives: In this study, the distribution of Legionella and its epidemiologic characteristics were analyzed in the water systems of public facilities in Chungcheongnam-do Province in South Korea. Methods: Culture and PCR analysis were performed on 2,991 environmental water system samples collected from 2017 to 2019, and associations with year, facilities, seasons, and temperature of water system were statistically analyzed by using R-Studio for Windows. Descriptive data was compared using chi-square tests and independent t-tests. Results: The detection rate of Legionella increased from 3.1% in 2017 to 10.3% in 2019, appearing most frequently in the order of public baths, large-scale buildings, hospitals, and apartments. It was detected mainly in summer from June to August, over 1.0×10³ CFU/L on average in 133 cases (66.5%). Lots of germs were detected in bathtub water, cooling tower water, and warm water (p<0.001), and it was detected at higher rates in the cities where multipurpose facilities were concentrated than in rural areas (p=0.018). Conclusions: This study suggests that continuous monitoring and control are required for Legionella in the water system environment of high risk facilities. Moreover, these results will be helpful to prepare efficient management plans to prevent the Legionellosis that occurs in Chungcheongnam-do Province.

• Korean Journal of Optics and Photonics
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• 제32권5호
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• pp.220-229
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• 2021

The optical characteristics of high-power direct-lit white light-emitting diode (LED) lighting were investigated, where a quantum dot (QD) film was adopted to enhance the color-rendering index (CRI). The transmittance of the diffuser plate and the concentration of the QD film were varied in this study. The color coordinates and the correlated color temperature (CCT) did not show any appreciable change, while the CRI values increased slightly as the transmittance of the diffuser plate decreased. The investigated optical properties were nearly independent of the viewing angle, and the luminance distribution was close to Lambertian. The CCT decreased from approximately 6000 K to approximately 4000 K as the concentration of the QD film increased from 0 to 7.5 wt%, which was due to the enhanced red component in the emission spectrum. The CRI increased to approximately 95 for some optical configurations of the lighting. These results demonstrate that glare-free, color-changeable, high-rendering LED lighting can be realized by using a combination of a diffuser plate of appropriate transmittance and a red QD film.

• Journal of Korean Society of Water and Wastewater
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• 제35권6호
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• pp.417-424
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• 2021

Algal bloom is an ongoing issue in the management of freshwater systems for drinking water supply, and the chlorophyll-a concentration is commonly used to represent the status of algal bloom. Thus, the prediction of chlorophyll-a concentration is essential for the proper management of water quality. However, the chlorophyll-a concentration is affected by various water quality and environmental factors, so the prediction of its concentration is not an easy task. In recent years, many advanced machine learning algorithms have increasingly been used for the development of surrogate models to prediction the chlorophyll-a concentration in freshwater systems such as rivers or reservoirs. This study used a light gradient boosting machine(LightGBM), a gradient boosting decision tree algorithm, to develop an ensemble machine learning model to predict chlorophyll-a concentration. The field water quality data observed at Daecheong Lake, obtained from the real-time water information system in Korea, were used for the development of the model. The data include temperature, pH, electric conductivity, dissolved oxygen, total organic carbon, total nitrogen, total phosphorus, and chlorophyll-a. First, a LightGBM model was developed to predict the chlorophyll-a concentration by using the other seven items as independent input variables. Second, the time-lagged values of all the input variables were added as input variables to understand the effect of time lag of input variables on model performance. The time lag (i) ranges from 1 to 50 days. The model performance was evaluated using three indices, root mean squared error-observation standard deviation ration (RSR), Nash-Sutcliffe coefficient of efficiency (NSE) and mean absolute error (MAE). The model showed the best performance by adding a dataset with a one-day time lag (i=1) where RSR, NSE, and MAE were 0.359, 0.871 and 1.510, respectively. The improvement of model performance was observed when a dataset with a time lag up of about 15 days (i=15) was added.

• Journal of the Korea Academia-Industrial cooperation Society
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• 제19권11호
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• pp.326-331
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• 2018

Evaporative humidification using a humidifying element is used widely for the humidification of a building or a data center. The performance of a humidifying element is commonly expressed as the humidification efficiency, which is assumed to be independent of the air temperature or humidity. To verify this assumption, a series of tests were conducted under two air conditions - data center ($25^{\circ}C$ DBT, $15^{\circ}C$ WBT) and commercial building ($35^{\circ}C$ DBT, $21^{\circ}C$ WBT) - using humidifying elements made from cellulose/PET and changing the frontal air velocity from 1.0 m/s to 4.5 m/s. Three samples having a 100 mm, 200 mm, or 300 mm depth were tested. The results showed that the humidification efficiency is dependent on the air condition. Indeed, even dehumidification occurred at the inlet of the humidifying element at the air condition of commercial building. This suggests that a proper thermal model should account for the inlet area, where the amount of moisture transfer may be different from the other part of the humidification element. As the depth of the element increased from 100 mm to 200 mm, the humidification efficiency increased by 29%. With further increases to 300 mm, it increased by 42%. On the other hand, the pressure drop also increased by 47% and 86%.

• Journal of Korean Tunnelling and Underground Space Association
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• 제21권4호
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• pp.535-543
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• 2019

This study examined the empirical meaning of skylight in underground space, and for this purpose, 29 questions were drawn, consisting of technical performance, functional performance, and behavioral performance. In addition, under the assumption that the meaning of the skylight will be clearly revealed through the comparison between the underground streets with and without the skylight, three underground streets with the skylight and three underground streets without the skylight of Japan were selected as the subjects of the study. Based on the results of the survey, descriptive statistical analysis and frequency analysis were conducted, and an independent sample T-test was conducted for each analysis, and correlation analysis was conducted to derive improvement priority. As a result of the analysis, it was found that skylight had a certain effect on the sense of time and imageability inside the underground commercial space. In addition, it was analyzed that skylight exerted important function in the qualitative aspect of underground commercial space. Lastly, the improvement rankings for three underground streets without skylight were derived in the order of sign/guide map, space diversity, maintenance, rest space, and floor design.The improvement rankings for three underground streets with skylight were drawn in order of resting space, diversity of space, temperature and humidity, air, and maintenance.

• Journal of the Computational Structural Engineering Institute of Korea
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• 제32권2호
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• pp.103-108
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• 2019

Non-equilibrium molecular dynamics simulation on the thermal boundary resistance(TBR) of an aluminum(Al)/silicon(Si) interface was performed in the present study. The constant heat flux across the Si/Al interface was simulated by adding the kinetic energy in hot Si region and removing the same amount of the energy from the cold Al region. The TBR estimated from the sharp temperature drop at the interface was independent of heat flux and equal to $5.13{\pm}0.17K{\cdot}m^2/GW$ at 300K. The simulation result was experimentally confirmed by the time-domain thermoreflectance technique. A 90nm thick Al film was deposited on a Si(100) wafer using an e-beam evaporator and the TBR on the film/substrate interface was measured using the time-domain thermoreflectance technique based on a femtosecond laser system. A numerical solution of the transient heat conduction equation was obtained using the finite difference method to estimate the TBR value. Experimental results were compared to the prediction and discussions on the nanoscale thermal transport phenomena were made.