• Food Science and Biotechnology
• /
• 제27권6호
• /
• pp.1811-1821
• /
• 2018

Obesity is generally caused by quantitative changes in adipocyte differentiation and fat metabolism. Only a few studies have been determined the effect of red beans extract on obesity and plasma cholesterol concentration. We have been studied the functional activities of red-bean extracts including anti-oxidative effect against DNA and cell damages. Histological study including micro CT analysis showed that the accumulation of fat in hepatocytes and intestines was significantly decreased in red bean extract treated group. In addition, plasma cholesterol and triglyceride levels were decreased in blood samples. In addition, it was confirmed that the red bean extract inhibited the expression of $PPAR{\gamma}$, Fabp4 and RETN genes, which regulate total adipocyte differentiation and lipid metabolism. Red bean extract inhibits the expressions of transcription factors associated with adipocyte differentiation in a dose-dependent manner, thereby inhibiting fat accumulation and decreasing blood lipid levels in obese mice induced by high fat diet.

• Biomolecules & Therapeutics
• /
• 제27권1호
• /
• pp.78-84
• /
• 2019

Cell therapeutic agents for treating degenerative brain diseases using neural stem cells are actively being developed. However, few systems have been developed to monitor in real time whether the transplanted neural stem cells are actually differentiated into neurons. Therefore, it is necessary to develop a technology capable of specifically monitoring neuronal differentiation in vivo. In this study, we established a system that expresses cell membrane-targeting red fluorescent protein under control of the Synapsin promoter in order to specifically monitor differentiation from neural stem cells into neurons. In order to overcome the weak expression level of the tissue-specific promoter system, the partial 5' UTR sequence of Creb was added for efficient expression of the cell surface-specific antigen. This system was able to track functional neuronal differentiation of neural stem cells transplanted in vivo, which will help improve stem cell therapies.

• Journal of Animal Science and Technology
• /
• 제50권4호
• /
• pp.475-484
• /
• 2008

본 연구는 retinoic acid(RA)가 돼지지방전구세포의 분화와 유전자 발현에 미치는 영향을 구명하기 위해서 수행하였다. 지방전구세포는 갓난 돼지의 등지방에서 분리했으며 RA는 배양중인 세포에 4일 동안 처리하였다. 배양중인 세포에서 RNA를 추출한 후 14,688개의 유전자가 부착되어 있는 cDNA microarray와 혼성화 하여 유전자 발현 양상을 분석하였다. 지방전구세포의 분화는 GPDH의 활성도에 의해 측정했다. RA는 돼지지방전구세포의 분화를 78% 억제했다. Retinoic acid 처리에 의해 지질 대사에 관계된 유전자를 포함하여 특히 sphin- gomyelin phosphodiesterase, apolipoprotein R precursor, growth factor receptor-bound protein 14, retinoic receptor RXR gamma의 발현이 증가 되었다. 그리고 세포 성장에 중요 역할을 하는 vascular endothelial growth factor D precursor, growth hormone receptor precursor의 유전자의 발현이 감소되었다. 이러한 결과는, RA가 성장촉진인자 또는 성장호르몬 수용체의 조절을 통해서 돼지 지방전구세포의 분화를 억제함을 나타낸다.

• 대한화학회지
• /
• 제53권4호
• /
• pp.471-475
• /
• 2009

• BMB Reports
• /
• 제43권5호
• /
• pp.369-374
• /
• 2010

The PC12 is the widely used cell line to study neuronal differentiation. We had extensively investigated the details of protein expression in differentiated PC12 cells by proteomic analysis. The cells were incubated at the presence of nerve growth factor. We had analyzed the expression changes in the differentiating PC12 cells by 2-dimensional electrophoresis and the identification of the proteins using MALDI-TOF MS. By comparing expression pattern in the time course, we identified the candidate genes which are associated with neuronal differentiation. Among these genes, we performed real-time PCR analysis to validate $Idh3{\alpha}$ expression by the time course. To identify the function of $Idh3{\alpha}$ in neuronal differentiation stage, the transfection of $Idh3{\alpha}$ to PC12 cells was performed. As a result, we proved that up-regulation of $Idh3{\alpha}$ causes reduction in neural differentiation of PC12 cells. Based on these data, we suggest that $Idh3{\alpha}$ plays a role to the neuronal differentiation.

• 지능정보연구
• /
• 제15권3호
• /
• pp.81-102
• /
• 2009

본 연구에서는 치열한 경쟁에 처해있는 인터넷 쇼핑몰(Internet Shopping Mall:ISM) 산업에서 생존을 위하여 필수적인 차별화 구현을 통한 수익성 향상 방안을 탐색한다. ISM 관리의 실질적 차별화를 가능하게 하는 실행 수준에서의 관리 요소를 분석하는 본 연구는 이제까지 주로 개념적이고 기업 수준에서 수행된 B2C 차별화 전략 연구와 ISM 고객만족에 영향을 주는 선행요건을 파악하는 실증적 연구를 효과적으로 결합하여 다음과 같이 진행 되었다. 첫째, 고객의 구매 의사결정 프로세스의 각 단계 별 거래비용 이론 관점에서 고객가치를 정의하였다. 둘째, 포괄적 문헌연구를 통하여 ISM 고객만족에 영향을 주는 요소들을 파악하고 이들 중 관리요소의 차별화가 가능하며 차별화를 통하여 고객 가치에 영향을 줄 수 있는 15개의 관리요소를 도출하고 연구모델과 가설을 수립하였다. 마지막으로 실증분석을 통하여 구조적 모델을 검증하고 차별화에 대한 시사점을 제시하였다. 본 연구는 ISM 차별화에 대한 실증적 연구로는 초기단계에 속하는 연구로 본 연구의 결과는 ISM 차별화를 위한 향후 연구와 실무적 응용에 유용한 시사점을 제시한다.

• 통합자연과학논문집
• /
• 제12권4호
• /
• pp.133-141
• /
• 2019

Mesenchymal stem cells (MSCs) are known to differentiate into multiple lineages, making neurogenic differentiation an important target in the clinical field. In the present study, we induced the neurogenic differentiation of cells using histone deacetylase (HDAC) inhibitors and studied their mechanisms for further differentiation in vitro. We treated cells with the HDAC inhibitors, MS-275 and NaB; and found that the cells had neuron-like features such as distinct bipolar or multipolar morphologies with branched processes. The mRNA expressions encoding for NEFL, MAP2, TUJ1, OLIG2, and SYT was significantly increased following HDAC inhibitors treatment compared to without HDAC inhibitors; high protein levels of MAP2 and Tuj1 were detected by immunofluorescence staining. We examined the mechanisms of differentiation and found that the Wnt signaling pathway and downstream mitogen-activate protein kinase were involved in neurogenic differentiation of MSCs. Importantly, Wnt4, Wnt5a/b, and Wnt11 protein levels were highly increased after treatment with NaB; signals were activated through the regulation of Dvl2 and Dvl3. Interestingly, NaB treatment increased the levels of JNK and upregulated JNK phosphorylation. After MS-275 treatment, Wnt protein levels were decreased and GSK-3β was phosphorylated. In this cell, HDAC inhibitors controlled the non-canonical Wnt expression by activating JNK phosphorylation and the canonical Wnt signaling by targeting GSK-3β.

• Asian-Australasian Journal of Animal Sciences
• /
• 제13권4호
• /
• pp.446-450
• /
• 2000

The experiment was conducted to study the effects of water soluble vitamins and retinoic acid on the differentiation of preadipocyte from omental, subcutaneous, intermuscular and intramuscular adipose tissue of Hanwoo. Differentiation was assessed by the change in enzyme activity, glycerol-3 phosphate dehydrogenase in serum free cell culture system. Preadipocytes treated with biotin ($10{\mu}M$) and pantothenic acid ($100{\mu}M$) were significantly (p<0.05) less differentiated than those from the control in all adipose tissue depots except intramuscular tissue. Although there was no significance, vitamin C was shown to stimulate the adipocyte conversion in omental and subcutaneous, but not in intermuscular and intramuscular adipose tissues. Lower values of GPDH activity in intermuscular preadipocyte were interpreted to be caused by relatively higher amounts of protein. In this experiment vitamin C did not stimulate fat deposition in intramuscular adipose tissue but further experiments are needed on the role of vitamin C in preadipocyte differentiation. When treated with different levels of retinoic acids, differentiation of preadipocytes was significantly (p<0.05) reduced from the level of $0.5{\mu}g/ml$ in omental and intermuscular, from $50{\mu}g/ml$ in subcutaneous, and in intramuscular at $500{\mu}g/ml$, thus showing that intramuscular preadipocytes were least responsive to retinoic acid in differentiation. All-trans retinoic acid appeared to inhibit the differentiation in a dose dependent manner, regardless of adipose tissues type.

• Toxicological Research
• /
• 제20권4호
• /
• pp.307-313
• /
• 2004

The present study was undertaken to investigate the effects of mistletoe (Viscum album var. coloratum) growing on Carpinus laxiflora BL. on proliferation and differentiation of HL-60 acute promyelocytic leukemia cells. Aqueous extract and its $(NH_2)_2SO_4$ saturated fractions of the mistletoe exhibited potent anti-proliferation activity against HL-60 cells. Moreover, when HL-60 cells were treated with 0~30% and 30～70% $(NH_2)_2SO_4$ saturated fractions of the mistletoe, HL-60 expressed CD 66b or CD 14 cell surface antigens and showed activity to reduce nitroblue tetrazolium, indicating that mistletoe induces the differentiation of HL-60 into granulocytes or monocytes. To understand how mistletoe induces the differentiation, we investigated the expression of molecules for modulating the proliferation and differentiation of leukemia cells, such as c-Myc and myeloblastin. The 0~30% $(NH_2)_2SO_4$ saturated fraction of the mistletoe reduced the mRNA levels of c-Myc and myeloblastin in a time-dependent manner. The results indicate that the mistletoe induces the differentiation of HL-60 cells via the decrease of c-Myc and myeloblastin expressions. Thus, it is suggested that mistletoe has a therapeutic potential for the treatment of acute promyelocytic leukemia.

• 한국건설관리학회:학술대회논문집
• /
• 한국건설관리학회 2007년도 정기학술발표대회 논문집
• /
• pp.441-445
• /
• 2007

정부의 대량주택공급정책 등으로 인한 고객중심으로의 시장변화는 자연히 주택 공급업체로 하여금 품질의 경쟁시대를 맞이하도록 하였다. 이러한 품질경쟁시대에서 빠르게 변화하는 고객의 요구품질에 부응하는 주택상품기획 방향을 모색하기위한 선행연구로서 현재 우리 주택시장에서 이루어지는 주택상품의 단위세대 품질의 차별화 사례유형에 대한 실태분석을 실시하였다. 조사 및 분석 결과 많은 사례들이 단순히 마감자재의 고급화나 일시 유행적인 친환경자재의 사용과 부분적이고 단속적인 생활편의품과 설비시스템의 건축요소와의 결합 등을 통한 상품풀질의 차별화를 꾀하고 있었다. 본 연구결과 주택상품의 차별화 기획은 고객을 중심으로 하여 단위세대의 품질 요소 중 건축지향적인 평면 및 인테리어요소와 기술지향적인 설비 및 제품요소의 유기적인 융합을 통합 품질의 차별화가 이루어져야 할 것으로 판단된다. 향후 주택상품기획단계에서는 고객 개념의 재정립을 통한 사람중심의 지속가능한 차별화 계획요소에 대한 연구 및 고객 지향적 주택상품기획 절차의 마련이 필요한 것으로 연구되었다.