• BMB Reports
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• v.46 no.5
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• pp.276-281
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• 2013

In this study, we aimed to compare the morphogenetic and neuronal characteristics between monolayer cells and spheroids. For this purpose, we established spheroid formation by growing SH-SY5Y cells on the hydrophobic surfaces of thermally-collapsed elastin-like polypeptide. After 4 days of culture, the relative proliferation of the cells within spheroids was approximately 92% of the values for monolayer cultures. As measured by quantitative assays for mRNA and protein expressions, the production of synaptophysin and neuronspecific enolase (NSE) as well as the contents of cell adhesion molecules (CAMs) and extracellular matrix (ECM) proteins are much higher in spheroids than in monolayer cells. Under the all-trans-retinoic acid (RA)-induced differentiation condition, spheroids extended neurites and further up-regulated the expression of synaptophysin, NSE, CAMs, and ECM proteins. Our data indicate that RA-differentiated SH-SY5Y neurospheroids are functionally matured neuronal architectures.

• Horticultural Science & Technology
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• v.19 no.4
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• pp.535-539
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• 2001

To establish the cultural practices of persimmon in heated plastic house, the growth and flower bud differentiation of 4-year old 'Nishimurawase' persimmon tree planted in 80 L pots were investigated. Temperature was kept at above $18^{\circ}C$. The earlier heating induced the earlier flower bud differentiation, while the number of days from bud burst to flower bud differentiation was 70-75 days, which was not affected by the date of heating. The date of flower bud differentiation in 'Nishimurawase' persimmon was May 1, May 21, and June 1 in the plastic house heated from Jan. 15, Feb. 15, and Mar. 15, respectively. The number of flower primordia per bud in the plastic house was 6.2-7.1, slightly fewer than that of 7.8 in the open-field, with the tendency of increasing the number by earlier heating. The days required for the differentiation of the last flower bud primordia from the beginning of differentiation of the first bud were 40-60, and that required for the flower bud differentiation of all buds on the same shoot was 30. The total carbohydrates in shoot increased and the contents of nitrogen decreased, resulting in increased C/N ratio just before flower bud differentiation, and it coincided with the time when the total carbohydates increased above 15%. During two weeks before flower bud differentiaton, The contents of zeatin per 1 g dry matter of shoot drastically increased from $27.2{\mu}g$ to $47.3{\mu}g$, while that of IAA slightly decreased from $188.6{\mu}g$ to $172.4{\mu}g$, and that of GA decreased from $2,225{\mu}g$ to $1,555{\mu}g$.

• Journal of the Korean Society of Environmental Restoration Technology
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• v.3 no.2
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• pp.1-9
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• 2000

Recently, the number of unsold apartments has been increasing, and apartment furnishing companies have tried a marketing strategy by utilizing the outdoor space for differentiation. There are many differentiation strategies. One of them is to improve the dwellings-on-ground space in apartment complex. Owing to the high density and high-rise of apartment buildings, the dwellings-on-ground apartment complex have been recognized as not good housing by residents in korea. The precedent study on the responses from residents showed the negative effects mainly due to sunlight, daylight, view and privacy and the positive effects from good accessibility and good environment for children and elderly people. The purpose of this study was to improve the dwellings-on-ground space and to suggest the most appropriate type of the dwellings-on-ground space through the residents' desires and preferences. In this point of view, this study shows three results for the design improvement: 1) Providing private gardens and individual accesses to the dwellings-on-ground. 2) Providing private garden with flower bed to intensify the visual aspect. 3) Furnishing variable transformation of unit plan in dwellings-on-ground, for example, maisonette, etc.

• Journal of Korean Society of Industrial and Systems Engineering
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• v.42 no.2
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• pp.49-61
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• 2019

As the technological gap amongst manufacturers decreases, the life cycle of products has shortened, and competition accelerates due to the development of technology, incumbent manufacturing companies face growth limitations. In order to overcome such business issues, manufacturing companies are increasingly interested in changes in business models and innovations, especially in the direction of providing services where they can maintain the competitive advantage of their products. In such context, this empirical study examines managerial leadership, differentiation strategies, and products and services pricing as 'servitization factors', which can be driving forces for moving into a new era of growth for Korean SMEs, focusing on the mediating effects of servitization competency. The results are as follows : First, it was confirmed that executive leadership, differentiation strategy, and information & communication technology capability have a direct effect on service sales. Second, the process competency, partnership competency, and ICT competency, which are presented as the service competence of SMEs, were found to play an important role in inducing service sales in managerial leadership, differentiation strategy, product and services pricing. It also emphasized the role of the public policy such as helping to foster SMEs as key partners in the expansion of social facilities and establishing platforms through ICT and data utilization for the convergence of manufacturing services.

• Korea Trade Review
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• v.44 no.4
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• pp.205-220
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• 2019

This article recapitulates the recent changes in trade laws, which may be accentuated due to the intriguing emergence of fortified protectionism and Mega FTAs. It points out the need to formulate not only the corporate strategy for enhancing the product differentiation and architectural capabilities but also the public policy, which comprises the industrial adjustment policy to cope with possible negative impulses caused by the digital trade and foreign direct investment. It is imperative for Korea to facilitate the alignment between corporate strategy and industrial adjustment policy as an effective means of enhancing industrial structure by nurturing those linkage effects between relevant forward and backward industries. Given the drastically volatile trade norms of multi-track trade policies, it may be a pivotal momentum for Korea to pursue a paradigm shift of its trade policy with a prime objective of achieving such an alignment between corporate strategy and industrial adjustment policy, which affords increased value-added and the further development of product or generic technology instead of resorting to the misuses and abuses of economies of scale and production technology for the maximization of export amount.

• Journal of the Korean Society of Fisheries and Ocean Technology
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• v.59 no.3
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• pp.253-262
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• 2023

To identify sand crab Ovalipes punctatus populations and establish management units for each population, mtDNA COI regions were analyzed. As a result, the clade of O. punctatus in Korea were separated by two with a genetic distance of 0.17-2.08%, and there was no significant difference in the result of pairwise FST values representing genetic differentiation by sampling areas (p > 0.05). Also, no geographical separation found in the distribution of haplotypes and the results of the haplotype network. This result suggests that O. punctatus larvae were dispersed for a long time by the ocean current by suffering meroplanktonic period for 1 month, and increased the gene flow due to the development of the swimming legs for the increase in mobility. Therefore, in the results of mtDNA COI region analysis of O. punctatus in the East Sea, Yellow Sea, South Sea and East China Sea (Ieodo) of Korea, no clear intra-species differentiation was found.

• Restorative Dentistry and Endodontics
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• v.45 no.4
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• pp.52.1-52.11
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• 2020

Objectives: Yttria-stabilized tetragonal phase zirconia has been used as a dental restorative material for over a decade. While it is still the strongest and toughest ceramic, its translucency remains as a significant drawback. To overcome this, stabilizing the translucency zirconia to a significant cubic crystalline phase by increasing the yttria content to more than 8 mol% (8YTZP). However, the biocompatibility of a high amount of yttria is still an important topic that needs to be investigated. Materials and Methods: Commercially available 8YTZP plates were used. To enhance cell adhesion, proliferation, and differentiation, the surface of the 8YTZP is sequentially polished with a SiC-coated abrasive paper and surface coating with type I collagen. Fibroblast-like cells L929 used for cell adherence and cell proliferation analysis, and mouse bone marrow-derived mesenchymal stem cells (BMSC) used for cell differentiation analysis. Results: The results revealed that all samples, regardless of the surface treatment, are hydrophilic and showed a strong affinity for water. Even the cell culture results indicate that simple surface polishing and coating can affect cellular behavior by enhancing cell adhesion and proliferation. Both L929 cells and BMSC were nicely adhered to and proliferated in all conditions. Conclusions: The results demonstrate the biocompatibility of the cubic phase zirconia with 8 mol% yttria and suggest that yttria with a higher zirconia content are not toxic to the cells, support a strong adhesion of cells on their surfaces, and promote cell proliferation and differentiation. All these confirm its potential use in tissue engineering.

• Proceedings of the Plant Resources Society of Korea Conference
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• 2023.04a
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• pp.69-69
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• 2023

Safflower seeds, classified as a member of the Asteraceae family, a dicotyledonous plant, contain linoleic acid as a major component, known for its pharmacological effect of strengthening bones. Additionally, safflower seeds have been reported to have pharmacological effects on vascular diseases such as atherosclerosis. In this study, we investigated the anti-obesity effect of safflower seed extract by examining its impact on adipocyte differentiation using Oil Red O staining, triglyceride quantification, and GPDH activity measurement. The results showed that safflower seed extract significantly inhibited adipocyte differentiation. Furthermore, we confirmed that safflower seed extract improved body weight, liver weight, adipose tissue size, glucose, and triglyceride levels in a high-fat diet-induced mouse model. These findings suggest that safflower seed extract exhibits potent anti-obesity activity both in vitro and in vivo and has the potential to be developed as a material for future anti-obesity therapies.

• ETRI Journal
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• v.40 no.3
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• pp.347-354
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• 2018

Using various offset times to separate differential services is the most common form of service differentiation in optical burst switching networks. In this approach, a larger offset time is given to a higher priority burst, but it causes this burst to have a longer delay. One solution to this problem is to adjust the burst assembly time so that the buffering delay of the higher priority burst is always shorter than that of the lower priority burst. However, this adjustment causes another problem, called delay unfairness, for bursts with differential priorities that share the same path to their destination. This article proposes a new solution for delay fairness using the burst assembly.

• Journal of Technology Innovation
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• v.13 no.1
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• pp.193-212
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• 2005

Leader-Member Exchange (LMX) model of leadership has suggested that members within the same work team are differentiated in terms of their level of the quality of leader member relationships. However, the differentiation in leader member relationships has not been examined for its possible team-level effects. In this study, the possible impact of this role differentiation on team members' affective reactions was explored. As hypothesized, teams whose members experienced higher level of LMX tended to have higher overall job satisfaction, organizational commitment, and team efficacy. Contrary to expectation, however, the team-level mean on LMX was not related with team-level variability on LMX. Based on these findings, several theoretical and practical Implications are discussed.