• Title/Summary/Keyword: Technology Combination

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Removal of Salmonella Typhimurium Biofilm from Food Contact Surfaces Using Quercus infectoria Gall Extract in Combination with a Surfactant

  • Damrongsaktrakul, Peetitas;Ruengvisesh, Songsirin;Rahothan, Arewan;Sukhumrat, Nuttamon;Tuitemwong, Pravate;Phung-on, Isaratat
    • Journal of Microbiology and Biotechnology
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    • v.31 no.3
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    • pp.439-446
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    • 2021
  • Quercus infectoria (nutgall) has been reported to possess antimicrobial activities against a wide range of pathogens. Nevertheless, the biofilm removal effect of nutgall extract has not been widely investigated. In this study, we therefore evaluated the effect of nutgall extract in combination with cetrimonium bromide (CTAB) against preformed biofilm of Salmonella Typhimurium on polypropylene (PP) and stainless steel (SS) coupons in comparison with other sanitizers. The minimum inhibitory concentration (MIC) and the minimum bactericidal concentration (MBC) of nutgall extract and surfactants (CTAB and sodium dodecyl sulfate; SDS) were assessed. CTAB showed a more efficient antimicrobial activity than SDS and was selected to use in combination with nutgall extract for removing biofilm. To determine the biofilm removal efficacy, the PP and SS coupons were individually submerged in 2x MBC of nutgall extract (256 mg/ml) + 2x MBC of CTAB (2.5 mg/ml), nutgall extract alone (256 mg/ml), CTAB alone (2.5 mg/ml), distilled water, and 100 ppm sodium hypochlorite for 5, 15, and 30 min. The remaining sessile cells in biofilm were determined. Overall, the greatest biofilm removal efficacy was observed with nutgall extract + CTAB; the biofilm removal efficacy of sanitizers tended to increase with the exposure time. The SEM analysis demonstrated that S. Typhimurium biofilm on PP and SS coupons after exposure to nutgall extract + CTAB for 30 min displayed morphological alterations with wrinkles. This study suggests nutgall extract + CTAB may be an alternative to commonly used sanitizers to remove biofilm from food contact surfaces in the food industry and household.

Lack of association between the VEGFA gene polymorphisms and preterm birth in Korean women

  • Yue Shi;Hyung Jun Kim;Seong Yong Kim;Ga Eun Kim;Han Jun Jin
    • Genomics & Informatics
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    • v.21 no.3
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    • pp.29.1-29.9
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    • 2023
  • Preterm birth (PTB), a pregnancy-related disease, is defined as a birth before 37 weeks of gestation. It is a major cause of maternal mortality and morbidity worldwide, and its incidence rate is steadily increasing. Various genetic factors can contribute to the etiology of PTB. Vascular endothelial growth factor A (VEGFA) gene is an important angiogenic gene and its polymorphisms have been reported to be associated with PTB development. Therefore, we conducted a case-control study to evaluate the association between VEGFA rs699947, rs2010963, and rs3025039 polymorphisms and PTB in Korean women. A total of 271 subjects (116 patients with PTB and 155 women at ≥38 weeks of gestation) were analyzed in this study. The genotyping of VEGFA gene polymorphisms was performed using polymerase chain reaction- restriction fragment length polymorphism. No significant association between the patients with PTB and the control groups was confirmed. In the combination analysis, we found a significant association between PTB and VEGFA rs699947 CC-rs2010963 GG-rs3025039 CC combination (odds ratio, 3.77; 95% confidence interval, 1.091 to 13.032; p = 0.031). The VEGFA rs699947, rs2010963, and rs3025039 polymorphisms might have no genetic association with the pathogenesis of PTB in Korean women. However, the combination analysis indicates the possibility that VEGFA acts in PTB pathophysiology. Therefore, larger sample sets and replication studies are required to further elucidate our findings.

Utilization of Soymilk Residue for Wheat Doenjang (밀가루 고오지에 의한 두유박이용 밀된장 제조)

  • Kim, Ze-Uook;Bang, Chan-Sik;Choi, Jun-Bong;Lim, Chun-Son
    • Applied Biological Chemistry
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    • v.32 no.4
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    • pp.357-361
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    • 1989
  • Wheat Doenjang was manufactured by mixing various ratio of the soymilk residue with wheat Koji. As the result, the content of reducing sugar was increased till 50 days and decreased thereafter during aging of the mash. As the combination ratio of soymilk residue was increased, the content of reducing sugar was decreased and amino nitrogen content was increased prominently till 50 days in all testing samples but thereafter the increasing ratio was slowdowned. At the beginning of the aging the content of the amino nitrogen was higher in the testing sample which had higher combination ratio of soymilk residue but at the end of the aging the result was reversed. The content of the total acids was increased prominently till 40 days but thereafter the increasing ratio was slowdowned, and in each testing samples the difference in total acid content was extremely slight. In the color of each testing samples aged 80 days as the combination ratio of soymilk residue was increased, the lightness was increased, and the redness was decreased but the yellowness was increased. From the result of sensory evaluation test of wheat Doenjang aged 90 days, soy sprout Doenjang soup showed the best taste in control and as the combination ratio of soymilk residue was increased, the taste was dropped slightly but no significant differences was obtained among the testing sample A,B and C but between control and testing sample D there was significant difference. In the original taste of Doenjang, the control was the best and as the combination ratio of soymilk residue increased, the point was dropped but there were no significant differences between control and testing sample A and testing sample B and C.

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Availability Analysis of Network RTK-GPS/GLONASS (Network RTK-GPS/GLONASS에 의한 지적측량 활용성 평가)

  • Lee, Jong-Min;Lee, In-Su;Tcha, Dek-Kie
    • Proceedings of the Korean Society of Surveying, Geodesy, Photogrammetry, and Cartography Conference
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    • 2010.04a
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    • pp.177-180
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    • 2010
  • In cadastral field GPS mainly applies to fundamental survey, while there are numerous research about cadastral detail survey using GPS application in order to increase surveying efficiency as survey technology improve. The purpose of this experiment is to analyze the accuracy of position and estimate the efficiency of GPS/GLONASS combination surveying with control points. As the result of this experiment, Network RTK-GPS/GLONASS combination survey is superior to Newtork RTK-GPS with respect to position accuracy and work efficiency.

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Synergistic Effects of the Combination of Oral Herbal Composition and Topical Hair Tonic on Hair Growth in C57BL6 Mice (경구용 생약 복합 조성물과 경피용 육모 조성물의 복합 사용에 따른 육모 상승 효과)

  • Jeon, Hee-Young;Kim, Seung-Hun;Shin, Hyun-Jeong;Seo, Dae-Bang;Lee, Sang-Jun
    • Korean Journal of Food Science and Technology
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    • v.44 no.2
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    • pp.257-262
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    • 2012
  • Currently, various treatments available for alleviating hair loss and combination treatments are commonly used, which are frequently questionable and in effective. We aimed to investigate the synergistic effect regarding the combination of oral herbal composition and topical hair tonic on anagen induction and hair restoration in a shaving model of C57BL/6 mice. Seven-week old mice were trimmed by electric clippers and treated with oral herbal composition and topical hair tonic either alone or in combination. The combination treatment showed the highest hair growth promoting effect. Moreover, it significantly improved total blood antioxidant capacity and reduced lipid peroxidation and triglyceride level, which was not observed in the topical hair tonic treated group. These results suggest that the combination of oral herbal composition and topical hair tonic has a synergistic hair growth promoting effect and such synergism may be the result of the differing hair regrowth mechanisms concerning treatments.

Thermal Durability Characteristics of Precious Metal(Pt) and Additives for a Catalytic Combustor (촉매연소기용 귀금속 촉매와 조촉매의 열적 내구특성 연구)

  • Choi, Byungchul;Ko, Byeongwoon;Kim, Myeonghwan;Sin, Hyeok
    • Journal of Institute of Convergence Technology
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    • v.10 no.1
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    • pp.19-24
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    • 2020
  • The purpose of the study is to investigate the thermal durability characteristics of the Pt catalyst and additives used in a catalytic combustor. The catalyst used in the experiment was based on Pt (3 wt%), and a total of 12 types were prepared using a combination of additives (Ni, La, Ce, Fe, and Co). From the results, In the fresh state, the two types of combination catalysts with the highest C3H8 conversion were Pt_Ce (79.9%) at 500℃, and in the three types of combination catalysts, Pt_La_Ni (93.4%) at 500℃ had the best performance. Among aged catalysts at 850℃ and 8 hours, Pt-La-Ni and Pt-Ni-Ce catalysts showed the highest C3H8 conversion of about 71% at 500℃.