• International Journal of Clinical Preventive Dentistry
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• 제14권4호
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• pp.216-221
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• 2018

Objective: In this study, we tried to investigate the antimicrobial activity of natural herbaceous plant-derived essential oils against oral disease-causing bacterial strains and establish the scientific evidences to apply them to the dentifrice, mouthwash, and other uses. Methods: We used the disk diffusion method with 23 types of 100% natural essential oils to verify the antimicrobial effect against Streptococcus mutans, Porphyromonas gingivalis, and Lactobacillus rhamnosus. After adhering to the surface of the plate culture medium, $20{\mu}l$ of the essential oil was prepared, and the diameter of the transparent ring was measured after incubation for 24 hours. All the experiments were repeated three times. Results: Among 23 types of oils, 13 were effective against all three strains: myrrh, ginger, basil, carrot seed, tea tree, patchouli, ylang ylang, cypress, lemongrass, cinnamon, peppermint, lavender, and eucalryptus. Seventeen oils were effective against S. mutans, and myrrh, basil, and carrot seed showed high antimicrobial activity. Eighteen oils were effective against P. gingivalis, and tea tree, carrot seed, and cinnamons showed high antimicrobial activity. Sixteen oils were effective against L. rhamnosus, and carrot seed and peppermint cinnamon showed high antimicrobial activity. Conclusion: Consequently, 13 essential oils showed the antimicrobial activity against three bacterial strains, which indicates these essential oils might be used as the effective materials to suppress the growth of oral-disease inducing microorganisms. Moreover, essential oils that have been analyzed activities in this study will be mixed up within the constant range and analyzed their antimicrobial effects to examine the synergistic activity among them.

• 대한임상검사과학회지
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• 제50권4호
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• pp.431-437
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• 2018

Acinetobacter baumannii는 광범위한 항생제에 대한 저항성으로 인해 감염된 환자의 사망률이 높아지는 적색 경보 병원체로 분류됩니다. 이 연구에서 다제 내성 A. baumannii(MRAB)의 18가지 임상 분리 균주에 대해 일부 에센셜 오일(티트리, 로즈마리, 라벤더 오일)의 항균 활성을 평가하고자 하였다. Carbapenemase 선별을 위한 Hodge 시험법은 A. baumannii의 20 가지 균주가 모두 imipenem에 내성이 있음을 보여주었습니다. 다제 내성 미생물의 확인은 VITEK 시스템을 통해 수행하였다. 에센셜 오일의 항균 활성은 MRAB에 대한 디스크 확산 방법으로 평가하였다. 디스크 확산 방법에서 tee tree는 라벤더 오일에 비해 억제 크기가 가장 크게 증가했으며, 로즈마리는 항균 효과가 없었다. 티 트리 오일은 가장 일반적인 인간 병원균 및 MRAB 감염의 치료 및 예방을 위한 대체 천연 제품으로 유용할 것으로 보인다. 따라서 이 연구의 결과는 다제 내성 A. baumannii의 항균 효과를 입증했으며, 미래에 천연 에센셜 오일을 사용하는 손 소독제와 같은 항균제로 사용될 것으로 예상됩니다.

• 생명과학회지
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• 제18권12호
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• pp.1644-1650
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• 2008

티트리 에센셜 오일은 호주 원주민들의 전통적인 피부 소독제나 치료제로 널리 사용되어 왔으며, 항균효과와 주요성분 등 많은 보고가 있으나 추출 방법이나 사용 부위 등에 따라 효능의 차이를 보인다. 본 연구에서는 아로마테라피 등에 현재 많이 이용되고 있는 시판 티트리 오일의 성분과 효능을 평가하여, 다른 에센셜 오일과의 비교 이용을 용이하게 하고자 하였다. 티트리 오일의 주요성분은 GC-MS 분석에 의하여 ${\beta}$-terpinene (20.87%), ${\alpha}$-pinene (17.60%), p-cymene (11.23%), 3-carene (10.40%), trans-anethole (8.47%), limonene (4.65%)으로 밝혀졌으며, 5% 이하의 농도에서 3시간 미만까지는 피부세포에 독성이 없었다. 오일의 라디컬 소거능을 알아본 결과, DPPH와 ABTS의 양라디컬에 대하여 강한 소거능을 나타내어 강한 항산화능을 시사했다. 또한, 오일의 direct contact와 vapor-phase의 항균활성을 disc diffusion법으로 스크리닝 한 결과, direct contact 활성의 경우 그람음성균에 대하여 높은 활성을 나타내었으며, vapor는 S. aureus에 대하여 강한 효과를 나타내었다. 본 연구에서 실제 많이 사용되는 티트리 오일의 성분과 생물활성을 측정함으로써 허브 오일들의 정확한 선택과 활용을 위한 기본적인 결과를 얻었다.

• Journal of Forest and Environmental Science
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• 제30권3호
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• pp.301-306
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• 2014

A study was carried out to identify the life cycle of Adoxophyes orana (Lepidoptera: Tortrididae) that inflicts tea tree leaves in Korea and selected three ecofriendly pesticides (Common name for commercial: Essential oil, Nemacatch, and Wormstop in Korean Farmers' Market) of A. orana for pest control. A. orana appeared to follow four life cycle phases a year; each presenting varying developmental periods dependent on seasonal and environmental factors. The fecundity of A. orana female was $24.6{\pm}4.10$ for $1^{st}$, $36.7{\pm}12.77$ for$ 2^{nd}$, and $27.9{\pm}4.22$ for $3^{rd}$ phase during 2011, while it was $65.0{\pm}32.72$, $49.7{\pm}30.27$, $63.8{\pm}27.22$ for corresponding phases during 2012. The average longevity of adult A. orana was 7.72 days. The average number of eggs deposited by each female in this study group was 44.62 with an average of 2.47. In three selected ecofriendly pesticides, the mortality of A. orana on treating with the Essential oil [The essential oil of Chamaecyparis obtuse (100%)] and Nemacatch [Azadirachtin 800-900 ppm (75%)] were 36.67% and 43.33% after 3 days and were 48.30% and 56.67% after 7days, respectively. Besides, the mortality of A. orana on treating with Wormstop [Azadirachtin 500 ppm (5%) and Salannin+Liminoids (95%)] was 61.67% and 78.33% after 3 and 7 days, respectively. Therefore, the application of Wormstop was the most useful to control the diseases caused by A. orana.

• 농업과학연구
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• 제47권4호
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• pp.1071-1081
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• 2020

Essential oils (EOs) have been shown to be plant-extracted antimicrobial agents. However, there are limited studies investigating the efficacy of EOs against pathogens. Among them, tea tree oil (TTO) is extracted from Melaleuca alternifolia, which is also used as an antifungal agent. In this study, the effect of TTO was investigated on the suppression of melon powdery mildew caused by Podosphaera xanthii and tomato leaf mold disease caused by Passalora fulva. Both powdery mildew and leaf mold diseases were significantly suppressed by a spray of TTO. Eighty percent of powdery mildew and 81% of leaf mold disease of the control value were suppressed by 0.5% TTO liquid, when sprayed 3 times every 7 days on the melon and tomato leaves. Inhibition of mycelial growth was also greatly affected by different concentrations of TTO against four different fungal pathogens. Ninety-eight percent of Pseudocercospora fuligena, 97% of P. fulva, 95% of Botrytis cinerea, and 94% of Phytophthora infestans mycelial growth were inhibited by 0.2% to 1.0% of TTO contained in plate media, respectively. However, phytotoxicity in plants by the TTO treatments was revealed when melon and tomato leaves were sprayed with a 1% and 2% concentration of TTO, respectively. Therefore, our findings show that TTO has high antifungal effects against various plant pathogens that occur during crop cultivation. We also suggest that when applying TTO to plant leaves, it is necessary to establish an accurate treatment concentration for different crops.

• 대한화장품학회지
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• 제25권4호
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• pp.17-41
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• 1999

Manuka oil sometime named New Zealand's tea tree oil is soluble in oil and come from nature. The $\alpha$-pinene extracted from Manuka oil and R-limonene which is one of the component of extracted Citrex from Grapefruit were used to estimate the antimicrobial activity and to improve the capability of antiseptic. Disk diffusion and broth dilution methods were used to measure the antimicrobial activity. Escherichia coli which is gram-negative bacteria and Staphylococcus aureus which is gram-positive bacteria were used as strain. The antimicrobial activity of Manuka oil and $\alpha$-pinene for Escherichia coli, Staphylococcus aureus is similar when the concentration of Manuka oil and $\alpha$-pinene is $10{\mu}l$. However, Antimicrobial activity of Manuka oil for Escherichia coli, Staphylococcus aureus is better than that of $\alpha$-pinene when the concentration of Manuka oil and $\alpha$-pinene is low. Antimicrobial activity of Citrex is superior to that of R-limonene. The proper ratio of Maunka oil and Citrex can improve the antimicrobial activity. The proper ratio obtained from studies was 75% of Maunka oil and 25% Citrex for Escherichia coli, 25% of Maunka oil and 75% Citrex for Staphylococcus aureus.

• 대한화장품학회:학술대회논문집
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• 대한화장품학회 1999년도 IFSCC . ASCS 학술대회 발표 논문
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• pp.17-41
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• 1999

Manuka oil sometime named New Zealand's tea tree oil is soluble in oil and come from nature. The $\alpha$-pinene extracted from Manuka oil and R-limonene which is one of the component of extracted Citrex from Grapefruit were used to estimate the antimicrobial activity and to improve the capability of antiseptic. Disk diffusion and broth dilution methods were used to measure the antimicrobial activity Escherichia coli which is gram-negative bacteria and Staphylococcus aureus which is gram-positive bacteria were used as strain. The antimicrobial activity of Manuka oil and $\alpha$-pinene for Escherichia coli, Staphylococcus aureus is similar when the concentration of Manuka oil and $\alpha$-pinene is 10${mu}ell$. However, Antimicrobial activity of Manuka oil for EscherEchta coli, Staphylococcus aureus is better than that of $\alpha$-pinene when the concentration of Manuka oil and $\alpha$-pinene is low. Antimicrobial activity of Citrex is superior to that of R-limonene. The proper ratio of Maunka oil and Citrex can Improve the antimicrobial activity. The proper ratio obtained from studies was 75% of Maunka oil and 25% Citrex for Escherichia coli, 25% of Maunka oil and 75% Citrex for Staphylococcus aureus.

• International Journal of Internet, Broadcasting and Communication
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• 제13권2호
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• pp.82-92
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• 2021

Purpose: It has been reported that the diversity and abundance of microbes in the vagina decrease due to the use of antimicrobial agents, and the high recurrence rate of female vaginitis due to this suggests that a new treatment is needed. Methods: In the experiment, we detected that 10% potassium sorbate solution, 1% eucalyptus oil solution, 1% tea tree oil solution, 400 µL/10 mL grapefruit seed extract solution, 100% lactic acid, 10% acetic acid solution, and 10% lactic acid solution were prepared and used. After adjusting the pH to 4, 5, and 6 with lactic acid and acetic acid in the mixed culture medium, each bacterium was inoculated into the medium and incubated for 72 h at 35℃. Incubate and 0 h each. 24 h. 48 h. The number of bacteria was measured after 72 h. Results: In the mixed culture test between lactic acid bacteria and pathogenic microorganisms, lactic acid bacteria showed good results at pH 5-5.5. Potassium sorbate, which has varying antibacterial activity based on the pH, killed pathogenic bacteria and allowed lactic acid bacteria to survive at pH 5.5. Conclusion: The formulation ratio obtained through this study could be used for the development of a feminine cleanser that can be used as a substitute for antibacterial agents. Further, the findings of this study may be able to solve the problem of antimicrobial resistance in the future.

• 한국패션뷰티학회지
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• 제2권3호
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• pp.19-26
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• 2004

석창포와 에센셜오일 Geranium(Pelargonum aspeum), lemongrass(Cymbpogen), Cypress(Cupressus Sempervirens), Chamomile Roman(Anthemis nobilis), Tea tree(malaleuca lterniflia), Bergamot(citrus aurant bergamia), lavender(Lavendula officinalis), Sandal wood(Santalum album)을 혼합하여 나타나는 항균 시너지 효과를 Streptococcus aureus, Pseudomonas aeruginosa, Candida albicans에 대하여 검토 해본 결과 다음과 같은 사실이 확인되었다. 1. 석창포는 Streptococcus aureus, Pseudomonas aeruginosa, Candida albicans에 대하여 뚜렷한 항균 효과를 나타냈다. 2. 아로마오일, Geranium(Pelargonum aspeum) lemongrass(Cymbpogen), Cypress(Cupressus Sempervirens), Chamomile Roman(Anthemis nobilis), Tea tree(malaleuca lterniflia), Bergamot(citrus aurant bergamia).lavender(Lavendula officinalis), Sandal wood(Santalum album)등은 Streptococcus aureus, Pseudomonas aeruginosa, Candida albicans에 대하여 강력한 항균 효과를 나타냈다. 3. 석창포와 아로마오일을 혼합 시에도 Streptococcus aureus, Pseudomonas aeruginosa, Candida albicans에 대하여 항균 시너지 효과를 나타냈다. 이로써 석창포와 아로마오일 모두 항균력을 가지고 있음이 확인되었고, 혼합시 또한 석창포와 아로마 오일의 대부분 상승효과가 있으며 이는 화장품에 폭넓게 응용할 수 있으리라 사료된다.

• 기본간호학회지
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• 제22권2호
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• pp.139-148
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• 2015

Purpose: This study was done to examine the effects of meatal care with essential oil on meatal E-coli and pH of inpatients in geriatric hospital. Methods: The participants were 40 patients admitted to J geriatric hospital in G city, Korea. Twenty patients were assigned to the experimental group and 20 to the control group. Participants in the experimental group received meatal care with essential oil (application of essential oil mixture consisting of lavender, tea tree, and frankincense). The control group received meatal care with saline. The meatal care was performed twice daily for one week in both groups. The scores for meatal odor, meatal pH and bacterial count for E-coli were measured before and after the treatment. Results: The score for meatal odor were significantly lower in the experimental group compared to the control group. The meatal pH and bacterial count for E-coli significantly decreased in the experimental group compared to the control group. Conclusion: Findings indicate that meatal care with essential oil is an effective nursing intervention to reduce meatal odor, meatal pH and bacterial count for E-coli for elderly women inpatients in geriatric hospitals.