• Korean journal of applied entomology
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• v.51 no.4
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• pp.389-395
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• 2012

Stored grain insects appear frequently as grains are harvested, stored, and distributed on the market. Producers mostly depend on chemical fumigants to control and repel insect pests, but this application of chemicals may cause side effects for humans and the environment. Safer control approaches, including the use of natural products are required, and in this study, we assessed the control effect of essential oils on stored grain insects. The essential oils of 8 plant species from 6 families were tested for their fumigant toxicity and repellent effects using a Y-tube Olfactometer against Sitophilus zeamais adults. Pennyroyal and tea tree oil respectively exhibited 97% and 63% mortality at $10{\mu}{\ell}L^{-1}$ air concentration in tested fumigant toxicity. Of particular note, pennyroyal oil ($LT_{50}$, hour=3.87) showed higher adulticidal activities than tea tree oil ($LT_{50}$, hour=4.09). When assessing repellent effects, using a Y-tube Olfactometer at $10{\mu}{\ell}$/filter paper concentration, the cedarwood, cinnamon, cypress, patchouli and pennyroyal essential oils showed potent repellent activities against Sitophilus zeamais adults. Cinnamon, cypress and pennyroyal essential oils showed notably high repellent activities of 82.4%, 80.0% and 86.7%, respectively, at $1{\mu}{\ell}$/filter paper concentration.

• Journal of the Korea Academia-Industrial cooperation Society
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• v.20 no.5
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• pp.102-108
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• 2019

Melaleuca alternifolia contains terpineol-4, cineol, sesquiterpenes etc., and has a germicidal effect and skin moisturizing effect. It also has the characteristics of relieving acne inflammation, treating dandruff, relieving pain, and relieving depression. In this study, an antimicrobial substance extracted from tea tree using an organic solvent (methanol, dichloromethane, ethyl acetate) and hydrothermal extraction method. And confirmed the antimicrobial activity of each extract. In order to verify the antimicrobial activity, nine pathogenic bacteria (Bacillus cereus, Bacillus subtilis, Listeria monocytogenes, Enterococcus faecalis, Staphylococcus aureus, Klebsiella pneumoniae, Escherichia coli, Salmonella typhimurium, Pseudomonas aeruginosa and Vibrio parahaemolyticus) were used. The antimicrobial activity of each extracts were confirmed by the commonly used disc diffusion method. The results showed that the fraction extracts of ethyl acetate and methanol had antimicrobial effects against V. parahaemolyticus and S. aureus. Using these results, we confirmed the antimicrobial activity of each fraction extracts and hot water extracts against V. parahaemolyticus. After the treat of samples, we confirmed at over 99.9 % of antimicrobial activity. In case of antifungal activities, we confirmed of preservation effect during over 45 hours. Based on the results of this research, further studies will be conducted to confirm the possibility of development as a new antimicrobial agent.

• Korean Journal of Clinical Laboratory Science
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• v.50 no.4
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• pp.431-437
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• 2018

Acinetobacter baumannii is categorized as a red alert pathogen that is increasingly associated with a high mortality rate in infected patients because of its resistance to extensive antibiotics. This study evaluated the antibacterial activities of some essential oils (tee tree, rosemary, and lavender oils) against 18 clinical isolates of multidrug-resistant A. baumannii (MRAB). The carbapenemase screening Hodge test showed that all 20 strains of A. baumannii were resistant to imipenem. The identification of multidrug-resistant microbes was carried out using the VITEK system. The antimicrobial activity of essential oils was tested by a disk diffusion method against MRAB. In the disk diffusion method, tea tree showed the largest increase in inhibition size compared to lavender oil, and rosemary had no antibacterial effect. These results proved the antimicrobial effect of multidrug resistance A. baumannii. Tee tree oil would be a useful alternative natural product for the treatment and prevention of most common human pathogens and MRAB infections. This is expected to be used as an antimicrobial agent, such as hand disinfectant using natural essential oil in the future.

• KSBB Journal
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• v.23 no.5
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• pp.387-391
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• 2008

Green tea is popular plants in Asian countries and has become more widespread in western countries due to its taste characteristics and health benefits. Apart from green tea leafs, however, the use of root and seed of green tea tree has not intensively been investigated yet. In this study, the contents of mineral, vitamin, total amino acid, free amino acid, and total polyphenol (catechin, caffeic acid, and chlorogenic acid) of the root and seed of green tea tree were analyzed for the development of healthy foods. For minerals, potassium contents were 1,052 and 1,480 mg/100g-dry weight of root and seed, respectively. The order of mineral contents were as follows: K > P > Ca > Mg > Na > Fe > Mn > Zn > Cu in root and K > P > Na > Ca > Mg > Mn > Fe > Cu > Zn in seed. For vitamins, vitamin C contents were 5.72 and 6.05 mg/100g-dry weight of root and seed, respectively. The presence of more various kinds of vitamins were observed in seed than in root. For total amino acids, the contents were 1,651 and 4,335 mg/100g-dry weight of root and seed, respectively. The total amino acid contents of seed and root were higher than those in commercial green tea products. Especially the phenylalanine contents were 16 and 139 mg/100g-dry weight of root and seed, respectively whereas phenylalanine was not found in commercial green tea products. Concerning free amino acids, the bitter tasting amino acids such as arginine, valine and tryptophan were more abundant in root and the sweet tasting ones such as glutamic acid, alanine, aspartic acid, and serine were more abundant in seed. The total polyphenol contents were 237 and 81 mg/100g-dry weight of root and seed, respectively. The polyphenol contents in root were three times higher than that in seed so root may be a better source for antioxidant ingredients than seed. Among many polyphenols, catechin, caffeic acid, and chlorogenic acid were the top three major components.

• Korean Journal of Food Science and Technology
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• v.9 no.1
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• pp.10-12
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• 1977

Chemical constituents and amino acid contents of tea leaves which were harvested three times (May, July and August) from the tree at two different locations were studied. The contents of total nitrogen, ascorbic acid and amino acid decreased, while those of fiber and tannin increased in the order of May, July and August crops. No differences were observed in the contents of reducing sugar, caffeine and ash among tea leaves harvested at different times. There were no effects of locations on the contents of chemical constituents of tea leaves.

• The Korean Journal of Ecology
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• v.27 no.1
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• pp.1-7
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• 2004

With the emergence and development of ecological engineering in China, and also the research and demonstration of Chinese Ecological Agriculture (CEA), in recent 10 years many types of ecological engineering have been appeared in Southern China, they could be classified into 4 typical patterns. These typical patterns are; 1. Ecological Building (EB), such as ecological public toilet, ecological garden, ecological integrated building, farmers' ecological household, the combine of ecological building and CEA; 2. Ecological Engineering in Paddy Field (EEPF), like rice-fish system, water caltrop-wild ricestem-fish system, mulberry-silkworm-pig-fish system; 3. Ecological Engineering of Agroforestry Systems (EEAF), as tea-food crops intercropping system, rubber-tea-chicken system, bamboo-chicken system, fruit tree-chicken system, metasequoia-fish-duck system; 4. Ecological Engineering Linked with Biogas (EELB), such as animal (pig)-biogas-fish system, animal (pig)-biogas-fruit tree system, animal-biogas-food crops system. Some case studies were presented in the paper. It is showed that these typical patterns have better ecological, social and economic benefits, now they are deeply been extending in southern China.

• Journal of the Society of Cosmetic Scientists of Korea
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• v.25 no.4 s.34
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• pp.17-41
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• 1999

Manuka oil sometime named New Zealand's tea tree oil is soluble in oil and come from nature. The $\alpha$-pinene extracted from Manuka oil and R-limonene which is one of the component of extracted Citrex from Grapefruit were used to estimate the antimicrobial activity and to improve the capability of antiseptic. Disk diffusion and broth dilution methods were used to measure the antimicrobial activity. Escherichia coli which is gram-negative bacteria and Staphylococcus aureus which is gram-positive bacteria were used as strain. The antimicrobial activity of Manuka oil and $\alpha$-pinene for Escherichia coli, Staphylococcus aureus is similar when the concentration of Manuka oil and $\alpha$-pinene is $10{\mu}l$. However, Antimicrobial activity of Manuka oil for Escherichia coli, Staphylococcus aureus is better than that of $\alpha$-pinene when the concentration of Manuka oil and $\alpha$-pinene is low. Antimicrobial activity of Citrex is superior to that of R-limonene. The proper ratio of Maunka oil and Citrex can improve the antimicrobial activity. The proper ratio obtained from studies was 75% of Maunka oil and 25% Citrex for Escherichia coli, 25% of Maunka oil and 75% Citrex for Staphylococcus aureus.

• Proceedings of the SCSK Conference
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• 1999.10a
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• pp.17-41
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• 1999

Manuka oil sometime named New Zealand's tea tree oil is soluble in oil and come from nature. The $\alpha$-pinene extracted from Manuka oil and R-limonene which is one of the component of extracted Citrex from Grapefruit were used to estimate the antimicrobial activity and to improve the capability of antiseptic. Disk diffusion and broth dilution methods were used to measure the antimicrobial activity Escherichia coli which is gram-negative bacteria and Staphylococcus aureus which is gram-positive bacteria were used as strain. The antimicrobial activity of Manuka oil and $\alpha$-pinene for Escherichia coli, Staphylococcus aureus is similar when the concentration of Manuka oil and $\alpha$-pinene is 10${mu}ell$. However, Antimicrobial activity of Manuka oil for EscherEchta coli, Staphylococcus aureus is better than that of $\alpha$-pinene when the concentration of Manuka oil and $\alpha$-pinene is low. Antimicrobial activity of Citrex is superior to that of R-limonene. The proper ratio of Maunka oil and Citrex can Improve the antimicrobial activity. The proper ratio obtained from studies was 75% of Maunka oil and 25% Citrex for Escherichia coli, 25% of Maunka oil and 75% Citrex for Staphylococcus aureus.

• International Journal of Clinical Preventive Dentistry
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• v.14 no.4
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• pp.216-221
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• 2018

Objective: In this study, we tried to investigate the antimicrobial activity of natural herbaceous plant-derived essential oils against oral disease-causing bacterial strains and establish the scientific evidences to apply them to the dentifrice, mouthwash, and other uses. Methods: We used the disk diffusion method with 23 types of 100% natural essential oils to verify the antimicrobial effect against Streptococcus mutans, Porphyromonas gingivalis, and Lactobacillus rhamnosus. After adhering to the surface of the plate culture medium, $20{\mu}l$ of the essential oil was prepared, and the diameter of the transparent ring was measured after incubation for 24 hours. All the experiments were repeated three times. Results: Among 23 types of oils, 13 were effective against all three strains: myrrh, ginger, basil, carrot seed, tea tree, patchouli, ylang ylang, cypress, lemongrass, cinnamon, peppermint, lavender, and eucalryptus. Seventeen oils were effective against S. mutans, and myrrh, basil, and carrot seed showed high antimicrobial activity. Eighteen oils were effective against P. gingivalis, and tea tree, carrot seed, and cinnamons showed high antimicrobial activity. Sixteen oils were effective against L. rhamnosus, and carrot seed and peppermint cinnamon showed high antimicrobial activity. Conclusion: Consequently, 13 essential oils showed the antimicrobial activity against three bacterial strains, which indicates these essential oils might be used as the effective materials to suppress the growth of oral-disease inducing microorganisms. Moreover, essential oils that have been analyzed activities in this study will be mixed up within the constant range and analyzed their antimicrobial effects to examine the synergistic activity among them.

• International Journal of Internet, Broadcasting and Communication
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• v.13 no.2
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• pp.82-92
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• 2021

Purpose: It has been reported that the diversity and abundance of microbes in the vagina decrease due to the use of antimicrobial agents, and the high recurrence rate of female vaginitis due to this suggests that a new treatment is needed. Methods: In the experiment, we detected that 10% potassium sorbate solution, 1% eucalyptus oil solution, 1% tea tree oil solution, 400 µL/10 mL grapefruit seed extract solution, 100% lactic acid, 10% acetic acid solution, and 10% lactic acid solution were prepared and used. After adjusting the pH to 4, 5, and 6 with lactic acid and acetic acid in the mixed culture medium, each bacterium was inoculated into the medium and incubated for 72 h at 35℃. Incubate and 0 h each. 24 h. 48 h. The number of bacteria was measured after 72 h. Results: In the mixed culture test between lactic acid bacteria and pathogenic microorganisms, lactic acid bacteria showed good results at pH 5-5.5. Potassium sorbate, which has varying antibacterial activity based on the pH, killed pathogenic bacteria and allowed lactic acid bacteria to survive at pH 5.5. Conclusion: The formulation ratio obtained through this study could be used for the development of a feminine cleanser that can be used as a substitute for antibacterial agents. Further, the findings of this study may be able to solve the problem of antimicrobial resistance in the future.