• Journal of Microbiology and Biotechnology
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• 제11권3호
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• pp.504-507
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• 2001

The partial 16S gene sequences of six filamentous cyanobacterial strains, Oscillatoria lmosa KCTC AG10168, Oscillatoria princeps KCTC AG10153, Oscillatoria sp. KCTC AG 10184, Phormidium tenue KCTC AG10158, Phormidium parchydematicum KCTC AG10164, and Lyngbya hieronymusii KCTC AG10199, which were isolated in the late summer at Daecheong Reservoirs, were determined and assigned their phylogenetic and taxonomic position among taxa of order Ocillatoriales whose partial 16S rRNA gene sequences aligned in this suty, were very heterogeneously clustered with other taxa. The two strains, Oscillatoria limosa KCTC AG10168 and O. princeps KCTC AG10153, formed a cluster with O. sancta PCC7515, which supported 64% of the bootstrap trees with high similarity (19-96.15%). Strain Oscillatoria sp. KCTC AG10184, that was known to produce a nasty substance, was closely related to the toxic Oscillatoria group. The study on morphological variation in various environments and toxin production will confirm the taxonomic status of these species. Phormidium tenus KCTC AG10158 and Phormidium parchydematicum KCTC AG10164 made a cluster with other oscillatorian species of Phormidium, Oscillatoria, and Leptolynbya, which supproted 100% of the bootstrap trees with a very high sequence smilarity (96.8-99.8%) in thsi study. The sequence analysis in this study also supported that taxa of oscillatoriales are not monophyletic. Some of the fractures, such as the presence or absence of sheath and cell shape, which were used to define them, would be inadequate and should be reconfirmed. We suggest that sequences of partial 16S rRNA gene fragments aligned in this study should be more useful than morphological features in the identification and reconfirmation of the taxonomic status of these oscillactorian cyanobacteria.

• Journal of Microbiology
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• 제40권2호
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• pp.109-118
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• 2002

The present work is aimed at providing additional new pure cultures of oxalate utilizing bacteria and its preliminary characterization for further work in the field of oxalate-metabolism and taxonomic studies. The taxonomy of 14 mesophilic, aerobic oxalotrophic bacteria isolated by an enrichment culture technique from soils rhizosphers, and the juice of the petiole/stem tissue of plants was investigated. Isolates were characterized with 95 morphological, biochemical and physiological tests. Cellular lipid components and carotenoids of isolates were also studied as an aid to taxonomic characterization. All isolates were Gram-negative, oxidase and catalase positive and no growth factors were required. In addition to oxalates, some of the strains grow on methanol and/or formate. The taxonomic similarities among isolates, reference strains or previously reported oxalotrophic bacteria were analysed by using the Simple Matching (S/ sub SM/) and Jaccard (S$\_$J/) Coefficients. Clustering was performed by using the unweighted pair group method with arithmetic averages (UPGMA) algorithm. The oxalotrophic strains formed five major and two single-member clusters at the 70-86% similarity level. Based on the numerical taxonomy, isolates were separated into three phenotypic groups. Pink-pigmented strains belonged to Methylobacterium extorquens, yellow-pigmented strains were most similar to Pseudomonas sp. YOx and Xanthobacter autorophicus, and heterogeneous non-pigmented strains were closely related to genera Azospirillum, Ancylobacter, Burkholderia and Pseudomonas. New strains belonged to the genera Pseudomonas, Azospirillum and Ancylobacter that differ taxonomically from other known oxalate oxidizers were obtained. Numerical analysis indicated that some strains of the yellow-pigmented and nonpigmented clusters might represent new species.

• Parasites, Hosts and Diseases
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• 제29권1호
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• pp.55-66
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• 1991

Heterophyidae과에 속하는 Matagenimus yokegawai, Metagonimus Miyata Type, M. takahashii, Heterophyes disbar, H. heterophyes, H. nocens, Heterophyepsis continua, Pygidiopsis summa, Stellanpchasmusfaltatus, Stittodora lari 등 총 21 Operational Taxonomic Unit(OTU)를 사용하여 수리분류학적 연구를 시도하였다. 형질은 절대 측정치가 아닌 상대적 비율을 주로 사용하였으며, 형질수는 26개를 사용하였다. 각 형질간의 유사도를 측정하여 상관계수를 계산한 후 Ward의 방법에 의한 집괴 분석 및 인자 분석을 수행하였다. 분석 결과 Metagonimus 속(genus) 그룹은 Metagonimus yokogawai, Metagonimus Miyata Type, M. takahashii 등 3개의 표현군으로 구분이 되었으며, Metagenimus Miyata Type은 M. takahashii 의 아종 수준으로 분류되었다. Heterophyes 속 그룹은 Heterophyes dispar, H. heterophyes, Jf. nocens 등 명확한 3개의 표현 군으로 구분되며, H. nocens는 H. heterophyes의 아종이 아닌 뚜렷한 종의 수준에서 분류됨을 나타내었다. 그 외의 종들도 역시 뚜렷한 표현 군으로 분류되었으며, 이로부터 흡충류에 있어서도 수리분류학적 방법에 의해 분류가 명화한 분류군의 한계를 규정하는데 많은 도움을 줄 수 있을 것으로 사료된다.

• Animal Systematics, Evolution and Diversity
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• 제13권3호
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• pp.233-246
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• 1997

남한의 15개 지역에서 채집된 재첩속(Corbicula) 4종간의 유전적 분화수준 및 계통 유연관계를 규명하고자 전기영동을 이용한 동위효소 분석을 실시하였다. 이들 4종 중 담수 산인 C. fluminea, C. leana, C. colorata 3종의 종내 집단간 유전적 근연치는 S=0.970 이상 (0.970∼1.000)으로 매우 가깝게 나타났으나 기수산인 C. japonica의 집단들은 Gp-1 유전자 에서 뚜렷한 대립인자 차이를 가지는 2개의 유전적 group으로 분리(S=0.873)되어 이에 대한 추가적인 분류학적 검토가 요구되었다. 각 종간의 유전적 근연치는 C. fluninea와 C. leana 가 S=0.689, C. fluminea와 C. colorata는 S=0.594 그리고 C. leana와 C. colorata는 S=0.737 로 나타나 이들 담수산 3종간의 유전적 차이는 매우 뚜렷하였다. 특히, 기수산인 C. japonica는 담수산 3종과의 평균 유전적 근연치가 S=0.370으로 가장 먼 유전적 근연관계를 나타내었다.

• 한국해양학회지
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• 제20권3호
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• pp.62-69
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• 1985

忠武灣에서 分離한 108個의 菌株에 대하여 고전적 분류방법과 수리적 분류방법을 비교하고, 균주간의 相關關係數를 구하여 유사성을 確認하였다. 수리적 분류에서는 고전적 분류와는 달리 Gram(+)와 Gram(-) 菌株가 전체 cluster에 分散되어있고, coccus와 rod가 분리되었으며, genus간의 分類는 수리적 방법과 고전적 방법사이에 連關性이 크게 나타났다. 下層에서 採取된 균주는 다른 層에 비해 균주간의 분류학적 유사성이 떨어지는 것으로 나타났다. 또한 灣 內部 (Group I)의 菌株와 外部 (Group II)의 菌株間의 거리는 각 Group內의 菌株間의 연관성보다 적은 것으로 나타났으며, 干潮時 海水에서 분리된 균주와 滿潮時 분리된 균주간에는 연관성이 적음이 확인 되었다.

• 한국미생물·생명공학회지
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• 제25권6호
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• pp.537-545
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• 1997

The aim of the present research program was to isolate a strain of actinomycetes producing antifungal substance. Soil samples were collected from various sites in Korea and a number of actinomycetes were isolated from the soil samples by applying selective agar for actinomycetes. Among isolates, a strain (NA -52) producing antifungal substance against Pyricularia oryzae was selected. Chemotaxonomic and numerical identification were carried out for the isolate. Fifty taxonomic unit characters were tested and the data were analyzed numerically using TAXON program. The isolate was identified as a synonym of streptomyces diastaticus belong to cluster No. 19 (Streptomyces diastaticus). But it showed a low similarity to S. diastaticus in simple matching coefficients, hence it was considered as one new species in Streptomyces.

• 한국패류학회지
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• 제13권1호
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• pp.29-36
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• 1997

Incilaria속 육삼패류의 분류를 위해 국재 7지역에서 채집된 100개체를 대상으로 수평전분전기 영동법을 이용한 동위효소 분석을 실시하였다. I. fruhstorferi와 I. bilineata 종내 집단의 유전적 근연치는 S=0.635와 S=0.482의 유전적 근연치를 나타내는 Incilaria sp. group이 형성되어 이들의 분류학적 위치에 대한 추가 연구가 요구되었다. 각 종간 유전적 근연치는 I. fruhstorferi와 I. bilineata 간에는 S=0.364이며, I. fruhstorferi와 Incilaria sp.는 S=0.427, I. bilineata와 Incilaria sp.는 S=0.3999로 나타나 Incilaria sp. group은 I. bilineata보다 I. fruhstorferi에 가까운 유전적 유연관계를 나타냈다.

• Mycobiology
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• 제41권4호
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• pp.248-251
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• 2013

Ganoderma is a cosmopolitan wood-rot basidiomycete that has been extensively studied for its pathogencity and medicinal properties. Identification of Ganoderma based on macro-microscopic features led to large number of synonyms which resulted in 250 taxonomic names. A Ganoderma species collected from Courtallam, Tamil Nadu was identified as G. cupreum. Phylogenetic analysis inferred from internal transcribed spacer rDNA region resolved the Indian isolate MYC1 as Ganoderma cupreum which clustered with Australian and Asian "cupreum" clade with 85% bootstrap support BS and shared 99% and 98% nucleotide similarity with Malaysian and Australian 'cupreum' respectively. This study represents the first molecular evidence of G. cupreum from Asian origin.

• Journal of Species Research
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• 제6권3호
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• pp.207-213
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• 2017

In order to investigate the indigenous prokaryotic species diversity of the Nakdong River system in Korea, fresh water samples from Hapcheonho Lake and Jinyangho Lake were analyzed for bacterial taxonomic diversity. The isolated bacterial strains were identified based on 16S rRNA gene sequences, and those exhibiting at least 98.7% sequence similarity with known bacterial species, but never reported in Korea, were selected as unrecorded species. Eleven unrecorded bacterial species were discovered in this study. The isolates were identified as Aquabacterium citratiphilum, Clostridium ghonii, Curvibacter delicates, Deinococcus depolymerans, Eubacterium moniliforme, Flavobacterium nitrogenifigens, Kineosporia mesophila, Luteibacter jiangsuensis, Microbacterium terricola, Rhizobium larrymoorei, and Sediminicoccus rosea belonging to the phyla Actinobacteria, Bacteroidetes, Firmicutes, Proteobacteria and Deinococcus-Thermus. The selected isolates were further characterized for cellular and colonial morphologies, growth conditions, physiological properties, and enzymatic activities. Descriptive information of these previously unrecorded species is also provided.

• 한국동물학회지
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• 제39권2호
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• pp.147-158
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• 1996

Since the floating goby, Choenogobius onnuloris, has intricate and diverse morphological variations, allozymic analysis at 25 loci was carried out for their populations in Korea to clarify its taxonomic status. A genetic assay carried out revealed that the floating gobies were clearly divided into three genetic groups (Tvpe-A, Tvpe-B and Tvpe-C) in Korea. Alternative alleles were fated at six loci (Aco, 6pgd, Ldh-1, Got-1, Gpf, Gp3l. Some loci had considerable heterogeneitv among three types and no evidence of gene exchange in slunpatric populations was found from statistical analyses. The genetic similarity (Rogers'S) among three types was lower than 0.80 and divergent time estimate indicates that they were speciated during 1.2-1.8 million year before present (WBP). ASso, these three types of C. unnuluris were distinguished morpholosicallv from each other by several characters such as band ornamentations. In conclusion, the evidences presented here support recognition of three types of Chuenogobius annularis as typical discrete species.