• The Korean Journal of Zoology
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• v.24 no.2
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• pp.91-98
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• 1981

It has been confirmed that two dendrograms resulted from two similarity matrices, average taxonomic distance and correlation coefficient matrices, are different with each other when cluster analyses were performed with 571 adults of deer mice, Peromyscus maniculatus using 30 morphometric characters. To choose one of two similarity matrices mentioned above in order to construct a dendrogram representing phenetic relationships among taxa, an objective method using the result from principal component analysis as a standard result to compare with two matrices has been suggested.

• Journal of Plant Biology
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• v.28 no.3
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• pp.179-190
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• 1985

A review of taxonomic treatments of the amentiferous plant taxa reveals diverse classification. It appears to be necessary to investigate new characteristics and attempt to determine the significance of these characteristics in terms of amentiferous systematics. Serological techniques have been empolyed in a systematic investigation of selected taxa of the Amentiferae: Betulaceae, Fagaceae, Juglandaceae, Myricaceae, and Salicaceae. Data by qualitative analysis of pollen proteins, double immunodiffusion and conventional immunoelectrophoresis, have proved to be valuable in delimitation of taxa tested. When the antisera against Alnus hirsuta, Betula platyphylla var. japonica, Quercus aliena, Q. dentata, Q. glauca were tested; Alnus and Betula have the greatest protein similarity to one another; and next to the rest of betulaceous genera. Relatively strong protein similarity obtained with most representatives of Quercus and Fagus when reacted with antisera against Alnus and Betula is very much in contrast to the weak protein similarity obtained for the genera of Juglandaceae, Myricaceae, particularly of Salicaceae Tested with the same antisera. When Quercus antisera were used for various genera, the weakest protein similarity was obtained with Populus.

• Journal of Life Science
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• v.11 no.2
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• pp.133-137
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• 2001

The molecular taxonomic relationship of nine species in the genus Moniliella Stolk & Dakin and Trichosporonoides Haskins & Spencer and six species of other yea나-like fungi was examined by sequencing analysis of large subunit rDNA D1/D2 variable domain. The fifteeen species fell into two major groups corresponding with their genetic relationships. The nine species of the genus Moniliella and Trichosporonoides were placed at the same cluster. similarity values based on the D1/D2 domain sequences were 45.4-100% among species of genus Moniliella, 45.2-84.4% among genus Trichosporonoides species, and 45.6-90.1% among species of genus Moniliella and Trichosporonoides. Identical sequence similarity was observed between M. suaveolens var. nigra and M. suaveolens. A colse relationship of M. mellis. and M. acetoabutens is observed. The result of this study provided and insight into the genetic origins of genus Moniliella and Trichosporonoides species as well as their genetic relationships. Genus Moniliella and Trichosporonoides are closely related to each other based on sequence analysis of the large subunit rDNA D1/D2 region and we suggest combination of the genus Moniliella and Trichosporonoides to single genus.

• Journal of Microbiology
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• v.40 no.4
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• pp.335-339
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• 2002

Partial sequences of 16S rRNA gene of five chroococcalian blue-green algal strains, Aphanothece nidulans KCTC AG10041, Aphanothece naegelii KCTC AG10042, Microcystis aeruginosa KCTC AG10159, Microcystis ichthyoblabe KCTC AG10160, and Microcystis viridis KCTC AG10198, which were isolated from water from the Geumgang River, were determined and were inferred their phylogenetic and taxonomic positions among taxa of order Chroococcales. Most taxa of Chroococcales whose partial 16S rRNA gene sequences were aligned in this study, are clustered with other related taxa. Aphanothece nidulans KCTC AG10041 and Aphanothece naegelii KCTC AG10042 made a cluster with other European species of these genera, which supported 100% of the bootstrap trees with a very high sequence similarity (97.4-99.4%) in this study. Three strains, Microcystis aeruginosa KCTC AG10159, M. ichthyoblabe KCTC AG10160, and M. viridis KCTC AG10198, formed a cluster with other Microcystis spp. supported 100 % of the bootstrap trees with a similarity of 97.0-99.9% except for two strains. However, this phylogentic tree made no resolution among the species of Microcystis spp. The topology of the tree reconfirmed the taxonomic status of three species of Microcystis, identified in this study based on the morphology, as three colonial types of Microcystis aeruginosa com. nov. Otsuka et al. (1999c). The genera of chroococcalian cyanophytes are heterogeneously clustered in these sequence analyses. We suggest that more molecular studies on the genera of Chroococcales with reference strains, widely collected from restricted geographic or environmental ranges, get accurate taxonomic or phylogenetic determinations.

• ALGAE
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• v.35 no.3
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• pp.293-301
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• 2020

The applications of DNA barcoding have a wide range of uses, such as in taxonomic studies to help elucidate cryptic species and phylogenetic relationships and analyzing environmental samples for biodiversity monitoring and conservation assessments of species. After obtaining the DNA barcode sequences, sequence similarity-based homology analysis is commonly used. This means that the obtained barcode sequences are compared to the DNA barcode reference databases. This bioinformatic analysis necessarily implies that the overall quantity and quality of the reference databases must be stringently monitored to not have an adverse impact on the accuracy of species identification. With the development of next-generation sequencing techniques, a noticeably large number of DNA barcode sequences have been produced and are stored in online databases, but their degree of validity, accuracy, and reliability have not been extensively investigated. In this study, we investigated the extent to which the amount and types of erroneous barcode sequences were deposited in publicly accessible databases. Over 4.1 million sequences were investigated in three largescale DNA barcode databases (NCBI GenBank, Barcode of Life Data System [BOLD], and Protist Ribosomal Reference database [PR2]) for four major DNA barcodes (cytochrome c oxidase subunit 1 [COI], internal transcribed spacer [ITS], ribulose bisphosphate carboxylase large chain [rbcL], and 18S ribosomal RNA [18S rRNA]); approximately 2% of erroneous barcode sequences were found and their taxonomic distributions were uneven. Consequently, our present findings provide compelling evidence of data quality problems along with insufficient and unreliable annotation of taxonomic data in DNA barcode databases. Therefore, we suggest that if ambiguous taxa are presented during barcoding analysis, further validation with other DNA barcode loci or morphological characters should be mandated.

• Korean Journal of Ichthyology
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• v.27 no.3
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• pp.165-182
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• 2015

The snailfishes (Scorpaenoidei: Liparidae) is a large group requiring taxonomic reviews because of a potential taxonomic confusion due to its great similarity in external morphology between species and a variation of its body color with size. Molecular phylogeny and taxonomic review were conducted for 10 species in 3 genera reported around the Korean waters, which facilitate understanding interrelationship within its family, and further management of fisheries resources. Molecular phylogeny using mitochondrial COI (mtCOI) and nuclear RAG2 (nRAG2) sequences indicates that the reciprocal monophyly of the three genera (Careproctus, Crystallichthys, and Liparis) were supported except Crystallichthys matsushimae, being closely related to Careproctus notosaikaiensis in only mtDNA COI topology. Liparis ingens Gilbert and Burke (1912) was reported as a distinct species differing from L. ochotensis. However, in the present study, comparing L. ingens from Korea with L. ochotensis from Japan and Russia, the two species were well corresponded in their morphology and molecule, therefore, we suggest L. ingens to be treated as a junior synonym of L. ochotensis.

• The Plant Pathology Journal
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• v.26 no.1
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• pp.83-89
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• 2010

Taxonomic position of 46 Korean isolates of Rhizoctonia solani which were classified into nine intraspecific groups by anastomosis and cultural characteristics was analyzed by randomly amplified polymorphic DNA (RAPD) and sequence analyses of the internal transcribed spacer (ITS) regions of ribosomal DNA. All the isolates within each group showed highly similar band patterns in RAPD. The ITS regions of the isolates within the same groups showed a high level of sequence similarity above 96.0% whereas similarities among different groups were below 94.4%. When compared with several reference strains of R. solani from foreign countries, all the Korean isolates were clustered with the foreign isolates belonging to the same groups in the phylogenetic tree. All six Korean strains of AG-4 were identified as HG-1 out of 3 subgroup of AG-4. We discussed taxonomic position of Korean isolates of R. solani and showed that sequence analysis with ITS regions could be a rapid and useful method for identification of intraspecific group of R. solani.

• Korean Journal of Agricultural Science
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• v.16 no.2
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• pp.163-168
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• 1989

Fourty-four isolates showed acetylene-reducing(nitrogenase)activity under the atmosphere of 89% Ar, 10% $C_2H_2$ and 1 % $O_{2{\cdot}}$, these nitrogen-fixing isolates characterized chemotaxonomically and their taxonomic status was disscussed; twenty-three isolates corresponded to Azospirillum. They were curved/spiral rods, gram negative, motile by a polar flagellum, and also utilized glucose in nitrogen free medild by a polar flagellum, and also utilized glucose in nitrogen free medium. but the cellular fatty acid composition and quinone system of these isolates showed quite different characteristics with reference strains. Therefore, the taxonomic status of this nitrogen-fixing bacteria is disscussed and a new species Azospirillum. Sixty forur isolates utilized C-l compounds such as methanol and formic acid. phenotypic and chemotaxonomic characteristics of methanol utilizing isolates were investigated and their taxonomic status was discussed; Twenty-one isolates corresponded to Hyphomicrobium and for the other regular rods and irregular rods utilizing isolates showed different cellular fatty acid composition. These isolates were grouped into 8 cluster analysis and similarity values based on correlation coefficients. Among these 8 clusters, two corresponded Pseudomonas and for the other were not decided.

• Language and Information
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• v.12 no.1
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• pp.27-65
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• 2008

This paper presents a set of methodologies for aligning hypernym-hyponym noun pairs between Korean and English, based on the EuroWordNet approach. Following the methods conducted in EuroWordNet, our approach makes extensive use of WordNet in four steps of the building process: 1) Monolingual dictionaries have been used to extract proper hypernym-hyponym noun pairs, 2) bilingual dictionary has converted the extracted pairs, 3) Word Net has been used as a backbone of alignment criteria, and 4) WordNet has been used to select the most similar pair among the candidates. The importance of this study lies not only on enriching semantic links between two languages, but also on integrating lexical resources based on a language specific and dependent structure. Our approaches are aimed at building an accurate and detailed lexical resource with proper measures rather than at fast development of generic one using NLP technique.

• Korean Journal of Plant Taxonomy
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• v.46 no.2
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• pp.175-186
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• 2016

The taxonomic status and scientific names of Artemisia gmelinii and its allied taxa have long been debated. To provide correct names and taxonomic status, A. gmelinii and its allied taxa were reviewed in this study based on morphological characters. After a comparison of original descriptions and type specimens, the names of A. freyniana f. discolor and A. freyniana f. vestita used in Korea were recognized as A. sacrorum var. sacrorum and A. sacrorum var. incana, respectively, based on the characters of the trichome on the leaf, the geographical distributions, the chromosome numbers, and the existence of a pinule connection on the exine of the pollen. A. iwayomogi, the scientific name misused as A. gmelinii, was combined and newly named A. sacrorum var. iwayomogi (Kitam.) M. S. Park & G. Y. Chung based on its morphological similarity to the A. sacrorum group. A description, illustration, and key for each of the taxa are provided here.