• 대한치과의사협회지
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• 제22권12호통권187호
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• pp.1083-1089
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• 1984

As a study to elucidate whether taste buds contain specific proteins, rat dorsal lingual epithelium was analysed by electrophoresis. The epithelium of the vallate papilla (with numerous taste buds), the area of the fungiform papilla 9with a few taste buds), and the area between vallate papilla and large filiform papilla (not containing taste buds) were strippled off by treatment with 0.7% EDTA. The epithelial protein was extracted by 1% SDS and 1% Mercaptoethanol in 0.01M phosphate buffer (pH7.2). Extracts were analysed by disc SDS-PAGE. Because the patterns of protein composition from each site were similar with each other as a whole, it is concluded that taste buds do not contain specific protein detected by SDS-PAGE in adult rat. But a protein on M.W. 49000 which lies in the area of molecular weight of keratin molecules was found only in the epithelium containing taste buds. This results suggested that the epithelium containing taste buds differentiate dissimilarly to the epithelium not containing taste buds.

• 대한소아치과학회지
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• 제24권3호
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• pp.688-703
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• 1997

The effect of glossopharyngeal nerve transection on the taste buds of the rat vallate papilla was examined by using the method of DNA nick-end labeling (TUNEL) and standard electron microscopic technique at 1, 3, 5, 7, 9 days after denervation. In general, the number and size of taste buds decreased as more days passed after denervation. They started decreasing on day 3 post denervation and virtually all taste buds were disappeared on day 9 post denervation. In studies using TUNEL method, TUNEL postive cells markedly increased in their numbers one day post denervation, as compared with controls. The number of apoptotic taste bud cells per taste bud profile was averaged to be 0.64 and 0.44 for day 1 and 3 post denervation, respectively, whereas it was 0.10 in controls. In electron microscopy, apoptotic cells were identified by the presence of condensed and fragmentary nuclei in a cytoplasm, which resulted in increased density. In control rats, only few apoptotic cells were found. On days 1 and 3 post denervation, nerve fibers almost disappeared from the taste buds and some apoptotic cells were apparent. On days 7 and 9 post denervation, a few taste bud cells were still present in the epithelium of the bottom of the trench wall of the vallate papilla and most of them showed apoptotic changes. The results indicate that the death of taste bud cells in normal conditions is controlled by apoptosis and the decrease and disappearance of taste buds after denervation is also caused by apoptosis of taste bud cells.

• 대한수의학회지
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• 제45권4호
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• pp.477-483
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• 2005

This study was carried out to investigate the morphological development of the tongue in the 60-, 90- and 120-day-old fetuses and neonates of Korean native goats by light microscopy. In 60-day-old fetuses, the tongue tissues were differentiated into epithelium, lamina propria, and muscle layer. The primordia of filiform, conical, lentiform, fungiform, and vallate papillae appeared by this time, and rudiments of taste buds were observed in the epithelia of the primordia gustatory papillae. The dorsal lingual epithelia were PAS positive. Collagenous fibers and blood vessels were present in the lamina propria. In 90-day-old fetuses, the gustatory glands were moderately positive for PAS and muscle fibers, and connective tissues were developed. In 120-day-old fetuses, many taste buds were observed in the gustatory papillae. The muscle bundles, collagenous fibers, blood vessels and gustatory glands were well developed. In neonates, over 40 taste buds were found in a transverse section of the vallate papillae. Muscle layers, blood vessels, collagenous fibers and gustatory glands were very well developed.

• Molecules and Cells
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• 제45권12호
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• pp.877-882
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• 2022

Taste sensation is the process of converting chemical identities in food into a neural code of the brain. Taste information is initially formed in the taste buds on the tongue, travels through the afferent gustatory nerves to the sensory ganglion neurons, and finally reaches the multiple taste centers of the brain. In the taste field, optical tools to observe cellularlevel functions play a pivotal role in understanding how taste information is processed along a pathway. In this review, we introduce recent advances in the optical tools used to study the taste transduction pathways.

• 한국수산과학회지
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• 제38권3호
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• pp.158-163
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• 2005

This paper deals with the histology of the barbels of striped sea catfish, Plotosus lineatus (Thunberg). This fish have eight noticeable barbels of two pairs on their maxillary and mandibular. Each barbel is composed of an epidermis, dermis and a central rod of cartilage. The epidermis in the middle part of the maxillary barbel is thicker than those on other parts, and formed of stratified epithelium which contains many cutaneous taste buds and a few small club cells. Number of taste buds increase on the middle and posterior part of each barbel. The dermis consists of loose connective tissue fibers which encloses blood vessels and bundles of nerve fibers. The barbels of this fish can be categorized into stiff and flexible types and are accessory, feeding and sensory structures. Thus we substantiate that they are gustatory receptor organs for this fish.

• 대한한방소아과학회지
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• 제24권1호
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• pp.1-8
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• 2010

Objectives This study is to find out how fungiform papillae count, which represents the density of taste buds, is related to eating behavior of children and adolescents. Methods The authors measured fungiform papillae count, height, and weight of 50 healthy children aged from 2 to 15. To evaluate the severity of anorexia, parents of the subjects answered modified version of Korean Children's Eating Behaviour Questionnaire (K-CEBQ). Children with weight of less than 50th percentile were assigned to low-weight group, while the others to high-weight group. Pearson's correlation test was conducted in order to evaluate the relationship between modified K-CEBQ score and fungiform papillae count. Results Low-weight children scored $29.8{\pm}9.1$, while high-weight children scored $24.5{\pm}7.1$. (p<0.05) on modified K-CEBQ Pearson's correlation coefficient between modified K-CEBQ score and fungiform papillae count was 0.503 (p<0.05) in low-weight group, 0.260 in high-weight group, and 0.339 (p<0.05) in total. However, there were no statistical significance in correlations between modified K-CEBQ score and percentile of weight, height, or BMI. Conclusions Severity of anorexia was correlated to the density of taste buds, especially in children who weighed less than average. The analysis on each single question indicated that children with high taste bud density had poor appetite not because of their inadequate digestive function, but because of their fastidious eating habit. Further study should be focused on finding out which specific aspect of appetite is related to the taste bud density.

• Animal cells and systems
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• 제15권3호
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• pp.197-202
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• 2011

The present study demonstrates the first-ever characterization of cell types that express the vanilloid receptor 1 (VR1) in the taste buds of rat circumvallate papillae. We performed electron microscopy to identify the subcellular location. The VR1 immunoreactivity was associated with the endoplasmic reticulum, cytoplasmic vesicles, and plasma membrane of taste cells. These results demonstrate the localization of the VR1 in membranous structures of the taste cells. We used double immunofluorescence histochemistry with taste cell type-specific markers to identify the cell types that express the VR1. The VR1 was detected in all functional taste cell types (Type I, Type II, and Type III cells). Together, our data suggest that the VR1 might play different roles according to the cell types within a taste bud.

• 한국어류학회지
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• 제17권1호
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• pp.36-42
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• 2005

한국의 메기과 (Siluridae) 어류의 수염을 조직학적으로 조사하였다. 메기, Silurus asotus의 상악 수염은 연골성 중축을 감싸는 진피와 표피로 구성 되었다. 표피층에는 점액세포, 과립형세포 및 미뢰가 있었다. 세포질이 약한 호염기성을 보이는 점액세포와 호염기성을 보이는 미뢰는 각각 표피의 표면과 표피 말단 부위에 위치 하였다. 표피와 진피는 기저막으로 구분되며 진피는 소성결합조직으로 구성 되었으며 신경세포와 혈관이 내재하고 있었다. 수염의 가장 내부에 연골성 중축이 존재 하였으며 근육층으로 둘러싸여 있었다. 미유기, S.microdorsalis의 상악 수염 구조는 메기의 상악 수염 구조와 비교시 별다른 차이는 보이지 않았으나, 상악 상부와 하부에서의 표피 % 두께 및 상악 상부와 중부에서의 미뢰 수에 있어 미유기는 메기에 비해 차이를 보였다. 본 연구결과 메기와 미유기의 상악 수염은 딱딱하고 굴절성형(stiff and flexible type)으로 파악 되었다.

• Fisheries and Aquatic Sciences
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• 제15권4호
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• pp.299-303
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• 2012

Barbel structure and regenerated barbel length in the juvenile Chinese longsnout catfish Leiocassis longirostris (G$\ddot{u}$nther), were evaluated. The barbles consisted of an epidermis, a dermis, and a central rod. The epidermis harbored taste buds, granular cells and epidermal cells. The taste buds were basophilic and situated along the distal portion of the epidermis. The dermis was composed of loose connective tissue containing blood vessels pigment cells. The innermost central region was cartilage enclosed within layers of muscle layers. After 30 days, the regenerated barbel length measured $0.92{\pm}0.404mm$ at $15^{\circ}C$ (regenerated growth curve: y = 0.5085x + 4.0678, $r^2$ = 0.9654, where y is regenerated length and x is experimental period in days), $1.88{\pm}0.521mm$ at $20^{\circ}C$ (y = 0.1806x + 4.808, $r^2$ = 0.9822), and $6.44{\pm}0.751mm$ at $25^{\circ}C$ (y = 0.0914x + 4.9918, $r^2$ = 0.9944). Fifteen days after amputation, the regenerated length was significantly longer at $25^{\circ}C$ than at 15 or $20^{\circ}C$ (P < 0.05). The barbels of the Chinese longsnout catfish was the tender and flexible type, and our experimental findings provide evidence of temperature-dependent regeneration. Additional investigation of the behavior and physiology of the Chinese longsnout catfish is needed, particularly histological studies of regenerated barbels and the measurements of the numbers of taste buds per barbel under various environmental conditions.

• 대한소아치과학회지
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• 제24권2호
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• pp.339-351
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• 1997

출생 후 성장 발육에 따른 흰쥐 유곽유두의 신경분포 및 변화를 관찰하기 위하여 생후 1, 5, 10, 15, 20, 25, 35일 및 완전히 성장한 흰쥐를 대상으로 calcitonin gene-related peptide (CGRP)를 이용한 면역조직화학적 방법으로 관찰하여 다음과 같은 결과를 얻었다. CGRP-면역양성 신경섬유들이 혀의 양측에서부터 유두 내로 진입하여 성장발육에 따라 그 분포밀도가 증가하다가 10일군부터는 상피의 직하방에 특히 풍부하게 분포하여 subepithelial plexus를 형성하고 있었다. 1일군에서는 CGRP-면역양성 신경섬유의 특징적인 양상인 염주알 모양이 명확히 관찰되었으나 그 이후로는 염주알 모양은 다소 감소하였고 하나의 선으로 보이는 경우도 있었다. 상피 내로 진입하는 CGRP-면역양성 신경섬유들은 5일군에서 드물게 관찰되었으며 taste pore를 가진 성숙한 미뢰가 처음으로 관찰되는 10일군에서 그 수가 증가하여 출생 후 15일군에서는 상피 전반에 걸쳐 풍부하게 관찰되었다. 그러나 그 이후 20일군부터는 미뢰가 존재하는 trench wall의 하방 2/3부위의 CGRP-면역양성 신경섬유는 감소하고 유두의 top surface와 trench wall의 상방 1/3에 CGRP-면역양성 신경섬유가 제한적으로 관찰되었다.