• BMB Reports
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• v.39 no.6
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• pp.709-716
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• 2006

Mammalian polo-like kinase 1 (Plk1) acts at various stages in early and late mitosis. Plk1 localizes at the centrosome and maintains this position through mitosis. Thereafter Plk1 moves to the kinetochore and midbody region, important sites during chromosome separation and cytokinesis. The catalytic domain of Plk1 is in the N-terminus region, whereas the non-catalytic region in the C-terminus of Plk1 has a conserved motif, named the Polobox. This motif is critical for Plk localization. EGFP proteins fused with the N-terminus and C-terminus of Plk1 localize in the nucleus and centrosomes, respectively. The core sequences of the polo-box (50 amino acids) also localize in Plk1 target organelles. To screen for domain-specific target proteins of Plk1, we constructed an N-terminal domain and a tandem repeat polo-box motif, and used them as templates in a yeast two-hybrid screen. The HeLa cell cDNA library indicated several proteins including the centrosome/kinetochore components or regulators, to be characterized as positive clones. Through in vitro protein binding analyses, we confirmed an interaction between these proteins and Plk1. The data reported from this study indicate that the N- and C- termini of Plk1 may function through recruitment and/or activation of domain-specific target proteins in dividing cells. Additionally, tandem repeats of the conserved core motif of the polo-box are sufficient for targeting and may be useful as a centrosome/kinetochore-specific targeting peptide.

• BMB Reports
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• v.39 no.1
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• pp.61-67
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• 2006

Molecular co-suppression phenomena are important to consider in transgene experiments. Embryogenic cells were obtained from immature cotyledons and engineered with two different gene constructs (pHV and pHVS) through particle bombardment. Both constructs contain a gene conferring resistance to hygromycin (hpt) as a selective marker and a modified glycinin (11S globulin) gene (V3-1) as a target. sGFP(S65T) as a reporter gene was, however, inserted into the flanking region of the V3-1 gene (pHVS). Fluorescence microscopic screening after the selection of hygromycin, identified clearly the expression of sGFP(S65T) in the transformed soybean embryos bombarded with the pHVS construct. Stable integration of the transgenes was confirmed by polymerase chain reaction (PCR) and Southern blot analysis. Seeds of transgenic plants obtained from the pHV construct frequently lacked an accumulation of endogenous glycinin, which is encoded by homologous genes to the target gene V3-1. Most of the transgenic plants expressing sGFP(S65T) showed highly accumulation of glycinin. The expression of sGFP(S65T) and V3-1 inherits into the next generations. sGFP(S65T) as a reporter gene may be useful to increase the transformation efficiency of transgenic soybean with avoiding gene co-suppression.

• Journal of The Korean Astronomical Society
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• v.29 no.spc1
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• pp.173-174
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• 1996

We have mapped $17 deg^2$ region toward Galactic anticenter in $^{12}CO$ J = 1 - 0 using the 3 mm SIS receiver on the 14 m telescope at Taeduk Radio Astronomy Observatory (TRAO). The region mapped in this paper is the first target of the Galactic AntiCenter CO Survey Project (GACCOS) and was selected comparing with IRAS Sky Survey Atlas (ISSA) images at 100${\mu}m$. Molecular emission of the target area is found to be very extended and is well matching with the FIR emission boundary. There are several pieces of clouds, and as some of spectra show several peaks, there seem to be several clouds overlapped in some directions. The Velocity of Local Standard of Rest ($V_{LSR}$) of the CO emission of the mapped region ranges from -20 to +10 km/s. It is also found that the two cloudlets located around I = $180^{\circ}$ have $V_{LSR}$ = -20 km/s, which is very abnormal. The peak antenna temperature of 13 K arises near the H II Region S241.

• Journal of Agricultural Extension & Community Development
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• v.19 no.3
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• pp.649-672
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• 2012

The government established Special Accounts for Balanced National Development in 2005, and the Metropolitan Council has enacted and executed ordinance relevant to special accounts to relieve regional unbalanced growth and to promote regional balanced growth. This study derived a political implication through the comparison analysis of the background of establishment and its purpose, the selection criteria of support target, finance scale and tax revenue finance, appropriation target and finance distribution, etc. on the basis of Chungcheongnam-do Special Accounts for Balanced Development and Jeollabuk-do Eastern part Special Accounts among the special accounts implemented in metropolitan council. The major conclusions of this study are as follows. First, the transition from the concept of balanced development to specialized development is necessary for the development of distressed region. Second, the pure wastefulness ratio of the local government's tax revenue finance of special accounts designed concentrating upon special accounts for Wide-area Autonomous Communities development should be gradually expanded. Third, Special Accounting budget should be supported to promote specialized development through the selection and concentration centering around comparatively advantageous resource within the region. Fourth, the strategic special accounts budget application system of cities and counties to utilize the projects of the government and the province to achieve the goal of cities and counties should be prepared.

• The Journal of the Acoustical Society of Korea
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• v.20 no.3
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• pp.91-97
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• 2001

Since the new field information of target signal is important in the development and verification of active sonar system, experimental method and simulation technique are widely used in order to analyze the detail characteristics of target scattered echoes. Therefore, in this paper, the scale target experiment is performed to develope and Improve the target signal simulation model. Since the experimental results show that the specular reflection is the major component among scattering mechanisms, the target signal simulation model based on the Geometric Optics Theory (GOT) is developed. Complex target is separated into simple shapes, known as canonical shape. The contribution from individual canonical shapes are summed with proper phase and amplitude to produce the target strength of the whole complex body. Simulated target signal is compared with the experimental results and discussed.

• Journal of the Korean Institute of Intelligent Systems
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• v.19 no.3
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• pp.304-310
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• 2009

In this paper, target extraction method in FLIR(forward-looking infrared) images based on fuzzy thresholding which used bi-modality and adjacency to determine membership value is proposed. The bi-modality represents how a pixel is classified into a part of target using distribution of pixel values in a local region, and The adjacency is a measure to represent how each pixel is far from the target region. First, membership value is calculated using above two measures, and then fuzzy thresholding is performed to extract the target. To evaluate performance of proposed target extraction method, we compare other segmentation methods using various FLIR tank image. Experimental results show that the proposed algorithm is a good segmentation performance.

• The Journal of Korean Institute of Electromagnetic Engineering and Science
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• v.15 no.6
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• pp.579-589
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• 2004

The radar signals are generally very sensitive to relative orientations between radar and target. Thus, the performance of a target recognition system significantly deteriorates as the region of aspect angles becomes broader. To address this difficulty, in this paper, we propose a method based on the multi-aspect information in order to improve the classification capability ever for a wide angular region. First, range profiles are used to extract feature vectors based on the central moments and principal component analysis(PCA). Then, a classifier with the use of multi-aspect information is applied to them, yielding an additional improvement of target recognition capability. There are two different strategies among the classifiers that can fuse the information from multi-aspect radar signals: independent methodology and dependent methodology. In this study, the performances of the two strategies are compared within the frame work of target recognition. The radar cross section(RCS) data of six aircraft models measured at compact range of Pohang University of Science and Technology are used to demonstrate and compare the performances of the two strategies.

• Journal of the HCI Society of Korea
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• v.6 no.1
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• pp.9-16
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• 2011

The previous researches of 3D object modeling have been performed in a limited environment where a target object only exists. However, in order to model an object in the real environment, we need to consider a dynamic environment, which has various objects and a frequently changing background. Therefore, this paper presents a segmentation and tracking method for a foreground which includes a target object in the dynamic environment. By using depth information than color information, the foreground region can be segmented and tracked more robustly. In addition, the foreground region can be tracked on the sequential images by referring depth distributions of the foreground region because both the position and the status in the consecutive images of the foreground region are almost unchanged. Experimental results show that our proposed method can robustly segment and track the foreground region in various conditions of the real environment. Moreover, as an application of the proposed method, it is presented a method for modeling an object extracting the object regions from the foreground region that is segmented and tracked.

• The Journal of Korean Institute of Communications and Information Sciences
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• v.38B no.9
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• pp.736-743
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• 2013

In IRST(infrared search and track) system, the small target detection is very difficult because the IR(infrared) image have various clutter and sensor noise. The noise and clutter similar to the target intensity value produce many false alarms. In this paper. We propose the adaptive detection method which obtains optimal target detection using the image intensity information and the prior information of target. In order to enhance the target, we apply the human visual system. we determine the adaptive threshold value using image intensity and distance measure in target enhancement image. The experimental results indicate that the proposed method can efficiently extract target region in various IR images.

• KSCE Journal of Civil and Environmental Engineering Research
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• v.33 no.1
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• pp.297-304
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• 2013

Due to its excessive $CO_2$ emissions, road transport sector becomes a target for emission reduction strategies. Although precise and reliable emissions inventories are necessary for evaluating plans and strategies, developing the region-wide inventory is a difficult task mainly because of a lack of data including travel patterns and modal volumes in the reginonal context. Most existing inventory methodologies employ fuel sale data within the target region, which ignores actual regional traffic patterns and thus not suited to its geographical context. To overcome these problems, this study develops region-wide $CO_2$ emissions inventory methodology by utilizing the Korea Transport DB (KTDB). KTDB provides a number of useful information and data, such as road network with which one can identify in and out trips over the entire region, traffic volumes of various modes, distance of travel, travel speed and so on. A model of equations that allow the computation of volume of $CO_2$ emitting from the road transport activities within the target region is developed. Using the model, numerical analyses are performed for the case of Busan Metropolitan City to demonstrate the applicability of the developed model. This study is indeed exploratory in the sense that using the existing data, it develops the $CO_2$ emissions inventory methodology which can produce better results than those from conventional fuel sales methodology. This study also suggests further reresarch directions to develop more refined methodologies in region-wide basis.