• BMB Reports
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• v.53 no.5
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• pp.278-283
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• 2020

Muscle fibers are generally formed as multinucleated fibers that are differentiated from myoblasts. Several reports have identified transcription factors and proteins involved in the process of muscle differentiation, but the roles of microRNAs (miRNAs) in myogenesis remain unclear. Here, comparative analysis of the miRNA expression profiles in mouse myoblasts and gastrocnemius (GA) muscle uncovered miR-3074-3p as a novel miRNA showing markedly reduced expression in fully differentiated adult skeletal muscle. Interestingly, elevating miR-3074-3p promoted myogenesis in C2C12 cells, primary myoblasts, and HSMMs, resulting in increased mRNA expression of myogenic makers such as Myog and MyHC. Using a target prediction program, we identified Caveolin-1 (Cav1) as a target mRNA of miR-3074-3p and verified that miR-3074-3p directly interacts with the 3' untranslated region (UTR) of Cav1 mRNA. Consistent with the findings in miR-3074-3p-overexpressing myoblasts, knockdown of Cav1 promoted myogenesis in C2C12 cells and HSMMs. Taken together, our results suggest that miR-3074-3p acts a positive regulator of myogenic differentiation by targeting Cav1.

• Genomics & Informatics
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• v.13 no.1
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• pp.15-24
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• 2015

Fatty acid synthase (FASN, EC 2.3.1.85), is a multi-enzyme dimer complex that plays a critical role in lipogenesis. This lipogenic enzyme has gained importance beyond its physiological role due to its implications in several clinical conditions-cancers, obesity, and diabetes. This has made FASN an attractive pharmacological target. Here, we have attempted to predict the theoretical models for the human enoyl reductase (ER) and ${\beta}$-ketoacyl reductase (KR) domains based on the porcine FASN crystal structure, which was the structurally closest template available at the time of this study. Comparative modeling methods were used for studying the structure-function relationships. Different validation studies revealed the predicted structures to be highly plausible. The respective substrates of ER and KR domains-namely, trans-butenoyl and ${\beta}$-ketobutyryl-were computationally docked into active sites using Glide in order to understand the probable binding mode. The molecular dynamics simulations of the apo and holo states of ER and KR showed stable backbone root mean square deviation trajectories with minimal deviation. Ramachandran plot analysis showed 96.0% of residues in the most favorable region for ER and 90.3% for the KR domain, respectively. Thus, the predicted models yielded significant insights into the substrate binding modes of the ER and KR catalytic domains and will aid in identifying novel chemical inhibitors of human FASN that target these domains.

• Proceedings of the Korean Society for Bioinformatics Conference
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• 2005.09a
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• pp.361-365
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• 2005

Over the past few years, the complex and subtle roles of microRNA (miRNA) in gene regulation have been increasingly appreciated. Computational approaches have played one of important roles in identifying miRNAs from plant and animals, as well as in predicting their putative gene target. We present a new approach of comprehensive analysis of the evolutionarily conserved element scores and applied data compression technique to detect putative miRNA genes. We used the evolutionarily conserved elements [19] (see more detail on method and material) to calculate for base-by-base along the candidate pre-miRNA gene region by detecting common conserved pattern from target sequence. We applied the data compression technique [20] to detect unknown miRNA genes. This zipping method devises, without loss of generality with respect to the nature of the character strings, a method to measure the similarity between the strings under consideration [20]. Our experience to using our new computational method for detecting miRNA gene identification (or miRNA gene prediction) has been stratified and we were able to find 28 putative miRNA genes.

• Journal of Korean Society of Rural Planning
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• v.18 no.4
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• pp.129-140
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• 2012

Land suitability assessment assesses development, farming, and conservation suitability, considering land's soil, location, and possibility for use. It also implement segmentation of management regions into production, conservation, and plan management area. It is evaluated as a very significant system in establishing a land use system of sustainable development and development after planning in the aspect that it can establish proper land use plan. This study developed a recommendation model for development in agent-based model that interacts with surrounding lands. It also tried to summarize the area characteristic analysis and the results of land suitability evaluation, targeting three ri's in Yesan-Gun, and analyze the model's applicability by selection results. In order to recommend area for development that considers the use of the surrounding lands, it calculated development possibility indices that considered the ratings of all the lands in the target areas for each parcel and simulated the model. As a result, selected three areas in target region were suitable areas for development in land suitability assessment. In detail, ratings of the recommended parcels were 3, 4, and 5 ratings. As a result of examining the land status, it showed that all the three areas were plan management areas, thus easy for development. It is judged that the model for recommending area for development suggested in this study can be used as important basic data for setting the direction for development when establishing a regional planning.

• Journal of Korean Society of Environmental Engineers
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• v.36 no.9
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• pp.614-623
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• 2014

Customers water quality complaints by foreign substance in local water service can be able to call the main cause bring the distrust for tap water and inhibiting the rate of drinking water. In this study, foreign substances were collected in the target region. Foreign objects were subjected to qualitative and quantitative analysis of compounds and elements components to reveal the cause of detection. Also, resolve the complaints by foreign substance and improve the reliability for tap water providing high quality water supply scheme. Collected substances at the water quality complaint area were included in inorganic compounds due to internal corrosion and aging pipeline, as well as organic compounds containing a large amount of carbon (C) and oxygen (O) component. To decide and reduce for foreign substance, objective assessment of pipe condition in target area was required.

• Proceedings of the Korea Water Resources Association Conference
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• 2008.05a
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• pp.75-81
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• 2008

The water shortage in mega cities in Asia, which face a rapid growth in urban population, is an outstanding problem. It is important, therefore, to accurately estimate the water balance in each city in order to use the limited water resources effectively. In this study, we estimated the potential water resources in し sixteen mega cities in Asia. The target cities were Delhi and Calcutta, India; Colombo, Sri Lanka; Dhaka, Bangladesh; Yangon, Myanmar; Bangkok, Thailand; Kuala Lumpur, Malaysia; Singapore; Jakarta, Indonesia; Hanoi, Vietnam; Beijing and Hong Kong, the People's Republic of China; Seoul, the People's Republic of Korea; Manila, the Philippines, and Sapporo and Tokyo, Japan. The potential water resources were estimated by subtracting the actual evaporation from the amount of rainfall. The actual evaporation was estimated using the potential evaporation obtained by Hamon's equation which requires the air temperature and the possible hours of sunshine. When the results of Hamon's and Penman's evaporation equations were compared, a considerable error appeared in the low latitude region. The estimation using Hamon's equation was corrected with the linear regression line of Hamon's and Penman's equations. A classification of the land cover was carried out based on satellite photographs of the target cities, and the volume of surface runoff for each city was obtained using the runoff ratios which depended on the land cover. As a result, the potential water resources in the above mega cities in Asia were found to be greater than the world average. However, the actual water resources which are available for one person to use are probably very limited.

• Journal of Navigation and Port Research
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• v.38 no.2
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• pp.141-146
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• 2014

NIR(New Inspection Regime, hereinafter NIR) relating to the PSC(Port State Contorl, hereinafter PSC) has implemented in Asia-Pacific region under Tokyo-MOU from last January 2014. The most noticable difference between NIR and existing TF(Target Factor, hereinafter TF) is to evaluate company performance and to conduct PSC with flexible inspection interval on the basis of Ship Risk Profile; High Risk Ship, Standard Risk Ship and Low Risk Ship. Particularly deficiencies related to ISM Code, more than 5 deficiencies and detention record have a serious effect on the evaluation of Ship Risk Profile. Therefore ship and company are requested to ensure proper implementation of Safety Management System for the changed PSC tendency. Also RO(Recognized Organization, hereinafter RO) and Administration respectively should take suitable actions different to previous measures, such as implementation of cooperation and support system between vessel and company, changing subject and object for avoiding Mannerism in SMS audit, strengthening supervision on RO by Administration and establishment of private-public Partnership network.

• The Journal of Korean Institute of Communications and Information Sciences
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• v.34 no.8A
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• pp.610-617
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• 2009

This paper proposes a cross-layer design combining adaptive modulation and coding (AMC) with truncated hybrid automatic repeat request (HARQ) for wireless multicast transmission, in order to increase the spectral efficiency while meeting the target quality-of-service (QoS). In the design, we provide the selection criterion of AMC so as to satisfy the target packet error rate (PER) of all users when the multicast data is received through the common channel and the number of retransmission is limited by the delay constraint of the service. The analytically derived results show that the cross-layer design using HARQ provides a better spectral efficiency than the AMC without HARQ by allowing retransmission and code combining. It is also observed that the design for multicast outperforms that for unicast in the mid to high signal-to-noise ratio region.

• Journal of the Institute of Electronics Engineers of Korea SC
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• v.37 no.4
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• pp.11-21
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• 2000

This paper proposes a new algorithm for a mobile robot that detects and tracks a moving target, keeping it in the view range of the robot. The sensor system consists of a camera and a line light source. The camera system is placed so that the line light projected into the ground from the light source forms its image along the horizontal center line of the CCD array of the camera. The deformation of the line image from the horizontal center line contains the information on existence of a moving object, its velocity, and position. Since the proposed algorithm reduces the region of the image to be searched, it reduces the computational complexity significantly comparing to the conventional image processing algorithms. The performance of the proposed algorithm has been tested by implementing on a mobile robot.

• Journal of Forest and Environmental Science
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• v.34 no.4
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• pp.304-312
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• 2018

This study investigates to identify the impact factors of timber production cost on the relationship between production cost components and revenues generated by evaluating the entire timber supply chain. In this research, selected 13 logging zones as target areas and classified 14 forest production cost factors, six groups. Additionally, established 13 externalenvironmental factors with related stakeholders and forestry experts. And the BCR (Benefit Cost Ratio) method was then used to analyze the economics of each zone. Filled up a checklist and rated using 5 point scale for each target region, and extracted major cost factors for the production economy of the item. The analysis of major cost factors in the timber production revealed that wood grab equipment usage fee was the first ranked and forest trees purchase cost was ranked in the $2^{nd}$Also, the $3^{rd}$ranking was logging expenses, and transport cost, which accounted for 84% of the total cost, was ranked in the $4^{th}$. In addition, the rock land ratio, slope, timber payment (forest trees purchase cost), special timber, ratio of timber, DBH (Diameter at Breast Height), and mixed forest ratio were the factors that most affected the timber supply chain cost.