• Korean Journal of Microbiology
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• v.39 no.3
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• pp.128-134
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• 2003

Transformation-associated recombination (TAR) cloning is based on co-penetration into yeast spheroplasts of genomic DNA along with TAR vector DNA that contains 5'- and 3'-sequences (hooks) specific for a gene of interest, followed by recombination between the vector and the human genomic DNA to establish a circular YAC. Typically, the frequency of recombinant insert capture is 0.01-1% for single-copy genes by TAR cloning. To further refine the TAR cloning technology, we determined the effect of GC content on target hooks required for gene isolation utilizing the $Tg\cdot\AC$ mouse transgene as the targeted region. For this purpose, a set of vectors containing a B1 repeated hook and Tg AC-specific hooks of variable GC content (from 18 to 45%) was constructed and checked for efficiency of transgene isolation by radial TAR cloning. Efficiency of cloning decreased approximately 2-fold when the TAR vector contained a hook with a GC content ～${\leq}23$% versus ～40%. Thus, the optimal GC content of hook sequences required for gene isolation by TAR is approximately 40%. We also analyzed how the distribution of high GC content (65%) within the hook affects gene capture, but no dramatic differences for gene capturing were observed.

• Journal of Korean Clinical Health Science
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• v.4 no.1
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• pp.504-509
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• 2016

Purpose. This study aims to present basic data to develop programs suitable to the characteristics of elderly people through a meta-analysis of effectiveness of variables related to psychological effects of programs of elderly people conducted in past 15 years. Methods. The analysis subjects are total 229 articles related to the psychological effects of programs for elderly people and submitted for master or doctor degrees or published in academic journals. CMA2 is used for the analysis. Results. The results of this study shows lower variables of the psychological effect promoting factor group that are highly effective for Life Satisfaction, Self-Efficacy, Self-Respect and Self-Integration and lower variables of suppressing factor group that are highly effective for Loneliness, Tension, Weakness, Anger and Depression. For the effect scale of psychological effect promoting factors, Arts is shown to be the highest followed by Exercise, Consultation, Recollection, Others and Recreation in order. For the effect scale of suppressing factors, Consultation is shown to be the highest followed by Recollection, Arts, Exercise, Others and Recreation) in order. Conclusions. This study has its own meaning for establishing integrated factors of psychological effects of programs for elderly people and presenting realistic data for planing programs suitable for various desires of elderly people.

• Journal of the Korea Institute of Information and Communication Engineering
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• v.7 no.8
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• pp.1644-1652
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• 2003

In this paper, we have analyzed that interference effect between wireless LAN(IEEE 802.11 a) and radar system in 5㎓ band. Recently, Korea frequency Authority is considering the frequency allocation of wireless LAN system about 5㎓ band that is previously used in radar system. The co­existence occurs interference problem between wireless LAN and radar system, so it is required to analyze the effect of co­channel interference. Accordingly, the frequency allocation could be predicted for wireless LAN system in 5㎓ band and the interference effect has been analyzed by simulation with the radar signal modeling. Simulation results which are presented by PER and EVM show that high SIR(20 ㏈) is required to achieve the target PER about 10­1.

• Proceedings of the KSPS conference
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• 2002.11a
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• pp.165-168
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• 2002

This research was conducted to investigate the priming effect in Korean and English word production by Korean speakers. Picture-naming with distractors was used as experimental task. The type of target language, the type of distractor language and SOA(Stimulus Onset Asynchrony) were used as variables. Cross-linguistic priming effect and within-linguistic priming effect were mesured to investigate bilinguals' conceptual system.

• Korean Journal of Cognitive Science
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• v.34 no.2
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• pp.85-110
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• 2023

The gaze cueing effect appears as detecting a target rapidly and accurately when the direction of others' gaze corresponds with the location of the visual target. The gaze cue can be affected by the orientation of the face. The gaze cueing effect is strong when the face is presented upright, but the effect has only been observed in some studies when the face is presented inverted(e.g., Tipples, 2005). This study aimed to examine whether the gaze can operate as a cue to guide attention with upright faces, and to add variables that can affect the gaze cue, such as the orientation of the face, the orientation of the background, and a time interval between the gaze cue and the target(SOA). Furthermore, it systematically manipulated these variables to explore whether the gaze cueing effect can be observed under the various conditions. The results showed a significant gaze cueing effect even on the inverted face, contrasting with previous studies. These findings were consistently observed when the background stimulus was absent(Experiment 1) and present(Experiments 2 and 3). However, there was no significant interaction in the orientations between the face and the background. Moreover, in the short SOA(150 ms), we found a significant gaze cueing effect in conditions of every face and background orientation, whereas there was no significant gaze cueing effect in the long SOA(1000 ms). By presenting a consistent observation of the gaze cueing effect under the short SOA(150ms) even in the inverted faces, the results of this study pose questions about the reliability and repeatability of previous studies that did not report significant results of gaze cueing effects in that faces. Furthermore, our results are meaningful in providing additional evidence that attention can be guided toward the direction of the gaze even in various directions of the face and background.

• Proceedings of the Earthquake Engineering Society of Korea Conference
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• 2000.04a
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• pp.367-374
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• 2000

This study investigates the effect of hysteretic characteristics to the Inelastic Demand Spectrum (IDS) which was expressed by an acceleration(Sa) and a displacement response spectrum (Sd). Elasto Perfectly Plastic(EPP) model is used in this study and inelastic demand spectrum (Sa vs, Sd) are obtained from a given target ductility ratio. For a given target ductility ratio IDS can be obtained by using nonlinear time history analysis of single degree of system with forth five recorded earthquake ground motions for stiff soil site. The effect EPP model under demand spectrum is investigated by ductility factor and natural frequency. According to the results obtained in this study IDS has dependency on ductility factor and natural frequency.

• Speech Sciences
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• v.9 no.1
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• pp.173-184
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• 2002

The question of Korean rhythmic type is still a controversial issue (syllable-timed; stress-timed; word-timed). As a step toward solving the question, an experiment was carried out to examine compression effects in Korean. There has been a general belief that the increase of the number of following or preceding syllables causes compression of a vowel (or syllable) in many languages, and a marked anticipatory compression effect can be especially indicative of stress timing. The purpose of this research, therefore, was to obtain some evidence to determine whether or not Korean is stress-timed. The durations of the target vowel/a/ of the monosyllabic word /pap/ were measured at both word and sentence level. In general, marked anticipatory and backward compression effects on the target vowel were observed across one-, two- and three-syllable words in citation form, whereas the effects were neither marked nor consistent at sentence level. These results led us to claim that Korean is not stress-timed.

• Journal of Ocean Engineering and Technology
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• v.30 no.3
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• pp.177-185
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• 2016

In this study, the model developed by Hong (2012) was modified to describe the consecutive docking/undocking situation and was also applied to investigate the effect on seawater circulation in Busan port by consecutive docking/undocking at the connecting bridge of Busan port. Numerical experiments for various docking/undocking cases were performed by dumping the initial concentration within Busan Port and indicated that the concentration in Busan port becomes steady state without numerical wiggles after sufficient time (at least 20 or 30 days). In addition, it was found that the seawater circulation under ship docking was slightly reduced in comparison with that under ship undocking, and the approach time to the target concentration under all the docking cases increased in comparison with the undocking case.

• Magazine of the Korean Society of Agricultural Engineers
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• v.34 no.1
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• pp.66-77
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• 1992

A prototype probabilistic approach to thickness design for asphalt pavement surface course was developed using first-order second moment probability model. The tensile strain (load effect) developing at the bottom of surface layer due to the wheel load and the critical strain (resistance) of asphalt concrete were used as random variables for pavement reliability analysis. Based on the parameters for load effect and resistance data collected from reference and field, simulated data were generated by Monte Carlo method for reliability evaluation of the pavement for a typical rural highway. Thickness of pavement surface course was defined in terms of target reliability of the pavement, growth factor of traffic, design life of pavement and resistance of the asphalt concrete to be placed on the pavement. According to these rationales, prototype thickness design chrats were sugested through example studies. From these, similar design charts can be developed for many pavements if appropriate data and target reliability are determined.

• Journal of the Korean Ceramic Society
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• v.31 no.3
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• pp.312-320
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• 1994

Effect of the laser shot number on the particulates density of the pulsed laser deposited YBa2Cu3O7-x thin films and the laser irradiated surface morphology of the YBa2Cu3O7-x bulk target have been investigated. Until 100 laser shots of cumulative irradiation, the films has the particulates density of ~103 mm-2. However, after 100 laser shots, the density was increased more than 10 times. This results has been explained by the change of particulate ejection path with the development of conical structure at the target surface.