• The Journal of the Acoustical Society of Korea
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• v.16 no.2
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• pp.58-65
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• 1997

A new automatic classification method with high and stable accuracy for time-series image data is presented in this paper. This method is based on prior condition that a classified map of the target area already exists, or at least one of the time-series image data had been classified. The classified map is used as a reference map to specify training areas of classification categories. The new automatic classification method consists of five steps, i.e., extraction of training data using reference map, detection of changed pixels based upon the homogeneity of training data, clustering of changed pixels, reconstruction of training data, and classification as like maximum likelihood classifier. In order to evaluate the performance of this method qualitatively, four time-series Landsat TM image data were classified by using this method and a conventional method which needs a skilled operator. As a results, we could get classified maps with high reliability and fast throughput, without a skilled operator.

• Journal of the Korean Institute of Navigation
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• v.10 no.1
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• pp.101-127
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• 1986

Transportation provides an infrastructure vital to economic growth, and it is an integral part of production . As a port is the interface between the maritime transport and domestic transport sectors, it certainly plays a key role in any economic development. Therefore, it is doubtless that inadequacy of a nation's port will depress the level of throughput, to the level where it fails to meet the target set by the national economic planning schemes. Korea is surrounded by the seas and the economic structure of Korea consists of processing trades, so that it cannot be overstated that substantial economy in maritime transport coasts can be achieved through the improvement of the port transport system. This paper treats the transportation process in Pusan Port by Queueing Simulation method, and the reasonable size of Pusan Port is suggested from the point of view of efficiency maximization. The results of the analysis are summarized as follows; 1) the utility rate is 47.91 percents in general piers, 85-52 percents in container piers, and waiting time 5.2hrs, in general piers, 0.8 hrs, in container piers, and the probability of maximum queue length 12 ships in general piers, 2 ships in container piers, and the probability of waiting is 44 percents in general piers, 8 percents in container pier. 2) in general piers, the improvement of app. 30 percents in port capacity is desirable for operating effectively concerning the current arrival rate. By introducing the traffic control ion container piers, there is no apparent necessity of port investment, but I is expected to reduce invisible congestion occurred along the waiting line. 3) On Pusan Port, the optimal utility rate and the optimal arrival rate for reducing waiting time are 3.5 to 4.0(hrs./ship) in general piers, 5.1 to 6.0(hrs./ship) in container piers.

• Genomics & Informatics
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• v.18 no.4
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• pp.42.1-42.9
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• 2020

Chromatin immunoprecipitation coupled with high-throughput DNA sequencing (ChIP-Seq) is a powerful technology to profile the location of proteins of interest on a whole-genome scale. To identify the enrichment location of proteins, many programs and algorithms have been proposed. However, none of the commonly used peak calling programs could accurately explain the binding features of target proteins detected by ChIP-Seq. Here, publicly available data on 12 histone modifications, including H3K4ac/me1/me2/me3, H3K9ac/me3, H3K27ac/me3, H3K36me3, H3K56ac, and H3K79me1/me2, generated from a human embryonic stem cell line (H1), were profiled with five peak callers (CisGenome, MACS1, MACS2, PeakSeq, and SISSRs). The performance of the peak calling programs was compared in terms of reproducibility between replicates, examination of enriched regions to variable sequencing depths, the specificity-to-noise signal, and sensitivity of peak prediction. There were no major differences among peak callers when analyzing point source histone modifications. The peak calling results from histone modifications with low fidelity, such as H3K4ac, H3K56ac, and H3K79me1/me2, showed low performance in all parameters, which indicates that their peak positions might not be located accurately. Our comparative results could provide a helpful guide to choose a suitable peak calling program for specific histone modifications.

• Journal of the Korea Academia-Industrial cooperation Society
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• v.22 no.1
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• pp.294-298
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• 2021

Recently, many electronic devices have been integrated with various kinds of wireless sensor network technologies that have been enabled with wireless network connections. These wireless sensor network devices have adopted various kinds of wireless network technologies. On the other hand, because each wireless network technology has its advantages and disadvantages, the target and purposes should be considered carefully at the beginning of the development. In particular, the approach to the magnetic sensor should be considered carefully because it has its own characteristic compared to general sensors. The magnetic field generates nonlinear data. This paper introduces the design aspects to reflect low cost and wearable devices to use in a wireless sensor network. In addition, this paper addresses how to select proper sensor network technology. As a result, wireless sensor network devices were integrated using Zigbee and showed the performance of the throughput.

• Animal Bioscience
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• v.35 no.7
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• pp.975-988
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• 2022

Objective: In this study, we aimed to identify long non-coding RNAs (lncRNAs) that play important roles in starvation stress, analyze their functions, and discover potential molecular targets to alleviate starvation stress to provide a theoretical reference for subsequent in-depth research. Methods: We generated a piglet starvation stress animal model. Nine Yorkshire weaned piglets were randomly divided into a long-term starvation stress group (starved for 72 h), short-term starvation stress group (starved for 48 h), and the control group. LncRNA libraries were constructed using high-throughput sequencing of piglet ileums. Results: We obtained 11,792 lncRNAs, among which, 2,500 lncRNAs were novel. In total, 509 differentially expressed (DE)lncRNAs were identified in this study. Target genes of DElncRNAs were predicted via cis and trans interactions, and functional and pathway analyses were performed. Gene ontology functions and Kyoto encyclopedia of genes and genomes analysis revealed that lncRNA-targeted genes mainly participated in metabolic pathways, cellular processes, immune system processes, digestive systems, and transport activities. To reveal the mechanism underlying starvation stress, the interaction network between lncRNAs and their targets was constructed based on 26 DElncRNAs and 72 DEmRNAs. We performed an interaction network analysis of 121 DElncRNA-DEmRNA pairs with a Pearson correlation coefficient greater than 0.99. Conclusion: We found that MSTRG.19894.13, MSTRG.16726.3, and MSTRG.12176.1 might play important roles in starvation stress. This study not only generated a library of enriched lncRNAs in piglets, but its outcomes also provide a strong foundation to screen key lncRNAs involved in starvation stress and a reference for subsequent in-depth research.

• Animal Bioscience
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• v.36 no.2
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• pp.175-190
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• 2023

Objective: The study was conducted to screen differentially expressed long noncoding RNA (lncRNA) in chickens by high-throughput sequencing and explore its mechanism of action on intramuscular fat deposition. Methods: Herein, Rose crown and Cbb broiler chicken embryo breast and leg muscle lncRNA and mRNA expression profiles were constructed by RNA sequencing. A total of 96 and 42 differentially expressed lncRNAs were obtained in Rose crown vs Cobb broiler chicken breast and leg muscle, respectively. lncRNA-ENSGALT00000046546, with high interspecific variability and a potential regulatory role in lipid metabolism, and its predicted downstream target gene 1-acylglycerol-3-phosphate-O-acyltransferase 2 (AGPAT2), were selected for further study on the preadipocytes. Results: lncRNA-46546 overexpression in chicken preadipocyte 2 cells significantly increased (p<0.01) the expression levels of AGPAT2 and its downstream genes diacylglycerol acyltransferase 1 and diacylglycerol acyltransferase 2 and those of the fat metabolism-related genes peroxisome proliferator-activated receptor γ, CCAAT/enhancer binding protein α, fatty acid synthase, sterol regulatory element-binding transcription factor 1, and fatty acid binding protein 4. The lipid droplet concentration was higher in the overexpression group than in the control cells, and the triglyceride content in cells and medium was also significantly increased (p<0.01). Conclusion: This study preliminarily concludes that lncRNA-46546 may promote intramuscular fat deposition in chickens, laying a foundation for the study of lncRNAs in chicken early embryonic development and fat deposition.

• Journal of Korean Society for Quality Management
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• v.51 no.2
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• pp.283-296
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• 2023

Purpose: The purpose of this study is to actually implement and verify whether welding defects can be detected in real time by utilizing deep learning AI solutions in the welding process of electric vehicle hairpin winding motors. Methods: AI's function and technological elements using synthetic neural network were applied to existing electric vehicle hairpin winding motor laser welding process by making special hardware for detecting electric vehicle hairpin motor laser welding defect. Results: As a result of the test applied to the welding process of the electric vehicle hairpin winding motor, it was confirmed that defects in the welding part were detected in real time. The accuracy of detection of welds was achieved at 0.99 based on mAP@95, and the accuracy of detection of defective parts was 1.18 based on FB-Score 1.5, which fell short of the target, so it will be supplemented by introducing additional lighting and camera settings and enhancement techniques in the future. Conclusion: This study is significant in that it improves the welding quality of hairpin winding motors of electric vehicles by applying domestic artificial intelligence solutions to laser welding operations of hairpin winding motors of electric vehicles. Defects of a manufacturing line can be corrected immediately through automatic welding inspection after laser welding of an electric vehicle hairpin winding motor, thus reducing waste throughput caused by welding failure in the final stage, reducing input costs and increasing product production.

• Journal of Intelligence and Information Systems
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• v.14 no.4
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• pp.31-46
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• 2008

A stacking yard of a container terminal is a space for temporarily storing the containers that are carried in or imported until they are carried out or exported. If the containers are stacked in an inappropriate way, the efficiency of operation at the time of loading decreases significantly due to the rehandlings. The remarshaling is the task of rearranging containers during the idle time of transfer crane for the effective loading operations. This paper proposes a method of planning for remarshaling in a yard block of an automated container terminal. Our method conducts a search in two stages. In the first stage, the target stacking configuration is determined in such a way that the throughput of loading is maximized. In the second stage, the crane schedule is determined so that the remarshaling task can be completed as fast as possible in moving the containers from the source configuration to the target configuration. Simulation experiments have been conducted to compare the efficiency of loading operations before and after remarshaling. The results show that our remarshaling plan is really effective in increasing the efficiency of loading operation.

• Proceedings of the Korean Society for Bioinformatics Conference
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• 2006.02a
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• pp.83-89
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• 2006

Cellular functions are carried out by a concerted action of biochemical pathways whose components have genetic interactions. Abnormalities in the activity of the genes that constitute or modulate these pathways frequently have oncogenic implications. Therefore, identifying the upstream regulatory genes for major biochemical pathways and defining their roles in carcinogenesis can have important consequences in establishing an effective target-oriented antitumor strategy We have analyzed the gene expression profiles of human liver cancer samples using cDNA microarray chips enriched in liver and/or stomach-expressed cDNA elements, and identified groups of genes that can tell tumors from non-tumors or normal liver, or classify tumors according to clinical parameters such as tumor grade, age, and inflammation grade. We also set up a high-throughput cell-based assay system (cell chip) that can monitor the activity of major biochemical pathways through a reporter assay. Then, we applied the cell chip platform for the analysis of the HCC-associated genes discovered from transcriptome profiling, and found a number of cancer marker genes having a potential of modulating the activity of cancer-related biochemical pathways such as E2F, TCF, p53, Stat, Smad, AP-1, c-Myc, HIF and NF-kB. Some of these marker genes were previously blown to modulate these pathways, while most of the others not. Upon a fast-track phenotype analysis, a subset of the genes showed increased colony forming abilities in soft agar and altered cell morphology or adherence characteristics in the presence of purified matrix proteins. We are currently analyzing these selected marker genes in more detail for their effects on various biological Processes and for Possible clinical roles in liver cancer development.

• Animal Bioscience
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• v.35 no.1
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• pp.115-125
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• 2022

Objective: Intramuscular fat (IMF) is a critical economic indicator of pork quality. Studies on IMF among different pig breeds have been performed via high-throughput sequencing, but comparisons within the same pig breed remain unreported. Methods: This study was performed to explore the gene profile and identify candidate long noncoding RNA (lncRNAs) and mRNAs associated with IMF deposition among Laiwu pigs with different IMF contents. Based on the longissimus dorsi muscle IMF content, eight pigs from the same breed and management were selected and divided into two groups: a high IMF (>12%, H) and low IMF group (<5%, L). Whole-transcriptome sequencing was performed to explore the differentially expressed (DE) genes between these two groups. Results: The IMF content varied greatly among Laiwu pig individuals (2.17% to 13.93%). Seventeen DE lncRNAs (11 upregulated and 6 downregulated) and 180 mRNAs (112 upregulated and 68 downregulated) were found. Gene Ontology analysis indicated that the following biological processes played an important role in IMF deposition: fatty acid and lipid biosynthetic processes; the extracellular signal-regulated kinase cascade; and white fat cell differentiation. In addition, the peroxisome proliferator-activated receptor, phosphatidylinositol-3-kinase-protein kinase B, and mammalian target of rapamycin pathways were enriched in the pathway analysis. Intersection analysis of the target genes of DE lncRNAs and mRNAs revealed seven candidate genes associated with IMF accumulation. Five DE lncRNAs and 20 DE mRNAs based on the pig quantitative trait locus database were identified and shown to be related to fat deposition. The expression of five DE lncRNAs and mRNAs was verified by quantitative real time polymerase chain reaction (qRT-PCR). The results of qRT-PCR and RNA-sequencing were consistent. Conclusion: These results demonstrated that the different IMF contents among pig individuals may be due to the DE lncRNAs and mRNAs associated with lipid droplets and fat deposition.