• Title/Summary/Keyword: Tannase

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Studies on the Utilization of several varieties of Acorn in Korea Part 1. Amino Acid Contents of Korean Acorn eliminated Tannin and Isolation of Strains producing tannin-hydrolyzing-enzyme (한국산 도토리의 이용에 관한 연구 제 1보 : Tannin 분해효소 생산균수의 검색, 한국재내식 방법에 의하여 Tannin을 제거한 도토리 분말의 일반성분 및 동단백질의 Amino acid 조성에 관하여)

  • 김창식;신응태
    • Microbiology and Biotechnology Letters
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    • v.3 no.1
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    • pp.17-22
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    • 1975
  • Studies on the elimination of tannin from the acorns containing tannin up to 9%, for the utilization of the acorns in Korea, carried out as follows: 1. The strain of Asp. niger sp. which yield the reasonable enzymatic activity of tannase was isolated from the rotten acorns. 2. The method of hydrolyzing tannin in the water by suspending, and agitatiag the acorn powder: followed by decantation, showed the best result aomng the conventional methods in the efficiency of removal of tannin and in the economy of the process by reducing the tannin content to 0.18% in 24 hours. 3. It was notable that the acorn'powder from which the tannin was eliminated contains various essential amino acids in relatively ample a mount.

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Changes of Properties in Cinnamon Extracts Prepared by Enzyme Hydrolysis and Addition of Salts, Sugars and Antioxidant Synergists (효소분해와 염과 당 및 항산화 작용 상승제의 첨가에 의한 계피 추출액의 특성 변화)

  • Kim, Na-Mi;Do, Jae-Ho;Lee, Jong-Soo;Kim, Woo-Jung
    • Applied Biological Chemistry
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    • v.37 no.4
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    • pp.272-276
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    • 1994
  • The dried cinnamon was extracted with enzymes, salts, sugars and additives in order to find the most effective extraction material. Enzymatic hydrolysis of cinnamon suspension with cellulase, hemicellulase, pectinase, ${\beta}-1.4-glucosidase$, tannase and lipase showed a little increase of their cinnamic aldehyde contents. Solid yield, antioxidant activity and degree of browning were increased in hemicellulase treatment. Acid and alkali extraction of cinnamon showed a some increase in solid yields and antioxidant activity was increased by addition of glucose and Na-ascorbate. Cinnamic aldehyde contents and degree of browning were increased in extraction with Na-citrate addition.

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Fermentative Characteristics and Antioxidant Activity of Aronia (Aronia melanocarpa) Makgeolli prepared with Ipguk cultured by Aspergillus awamori (Aspergillus awamori 입국으로 제조한 Aronia 막걸리의 발효특성 및 항산화 활성)

  • Lee, Kyung-Haeng;Yun, Ji-Sun;Hwang, Jong-Hyun
    • The Korean Journal of Food And Nutrition
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    • v.31 no.1
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    • pp.117-126
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    • 2018
  • Aronia makgeolli was manufactured using Asp. kawachi (AK) and Asp. awamori (AA) with tannase activity, and physicochemical properties were examined during the fermentation period. The pH was decreased during the fermentation period after the first day, while the acidity increased. Reducing sugars increased highly on the first day of fermentation, and then they gradually decreased as the fermentation period elapsed. On the 7th day of fermentation, it was in the range of 0.38~0.61%. The alcohol content gradually increased during the fermentation period and it ranged from 13.4~14.2 v/v% by the 7th day of fermentation, and the alcohol content of makgeolli added with Aronia was somewhat lower than that of makgeolli prepared without aronia. The L value increased as the fermentation period elapsed, and the L value of makgeolli added with aronia increased rapidly. The a value gradually decreased, while the b value gradually increased as the fermentation period elapsed. The content of total polyphenols increased during the fermentation period of AK makgeolli. However, the AA makgeolli was not significantly increased, as compared to the initial stage of fermentation, and it was lower than that of the AK makgeolli. The radical scavenging activity of the DPPH was higher in the makgeolli added with aronia, and the antioxidant activity of AA makgeolli was higher than that of AK makgeolli. In the sensory evaluation, in the AK makgeolli, the palatability deteriorated due to the bitter taste and the astringent taste derived from the aronia. However, in the AA makgeolli, astringent taste was very weak and the sensory quality was good.

Multifarious activities of cellulose degrading bacteria from Koala (Phascolarctos cinereus) faeces

  • Singh, Surender;Thavamani, Palanisami;Megharaj, Mallavarapu;Naidu, Ravi
    • Journal of Animal Science and Technology
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    • v.57 no.7
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    • pp.23.1-23.6
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    • 2015
  • Cellulose degrading bacteria from koala faeces were isolated using caboxymethylcellulose-Congo red agar, screened in vitro for different hydrolytic enzyme activities and phylogenetically characterized using molecular tools. Bacillus sp. and Pseudomonas sp. were the most prominent bacteria from koala faeces. The isolates demonstrated good xylanase, amylase, lipase, protease, tannase and lignin peroxidase activities apart from endoglucanase activity. Furthermore many isolates grew in the presence of phenanthrene, indicating their probable application for bioremediation. Potential isolates can be exploited further for industrial enzyme production or in bioremediation of contaminated sites.

Characteristics of Bacteriocin Produced by a Lactobacillus plantarum Strain Isolated from Kimchi (김치에서 분리한 Lactobacillus plantarum 균주가 생산하는 박테리오신의 특성)

  • Chung, Jae Hyuk;Bae, Yun-Sook;Kim, Yeon-Joo;Lee, Jong-Hoon
    • Microbiology and Biotechnology Letters
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    • v.38 no.4
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    • pp.481-485
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    • 2010
  • Lactobacillus plantarum strain KK3 with tannase activity was isolated from Gochunipkimchi (red pepper leaf kimchi) and showed a high antagonistic activity against five kinds of food pathogens. Strain KK3 secreted antibacterial compound into culture medium and 24-h culture in MRS broth at $30^{\circ}C$ was enough for the antibacterial compound production. The crude antibacterial compound prepared from culture supernatant inhibited the growth of some Gram-negative bacteria and Bacillus cereus but not Listeria monocytogenes. The antibacterial activity was sensitive to proteinase K treatment, confirming its proteinaceous nature (bacteriocin). The crude bacteriocin was active in the pH range 3.5-8.5 and extremely stable after 15 min of heat treatment at $121^{\circ}C$. The strain KK3 produced equally active bacteriocin in Chinese cabbage juice as it produced in MRS broth.