• Proceedings of the Korean Aquaculture Society Conference
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• 2005.05a
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• pp.30-30
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• 2005

• Korean Journal of Fisheries and Aquatic Sciences
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• v.47 no.4
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• pp.390-398
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• 2014

We conducted an 8-week feeding trial to evaluate dietary lipid sources on the growth performance and body composition of juvenile river puffer fish Takifugu obscurus. Nine experimental diets were formulated with fishmeal as the major protein ingredients, providing 50% crude protein. The experimental diets contained either beef fallow (BF), soybean oil (SO), rapeseed oil (RO), or linseed oil (LO). Each of these diets was then supplemented or not with 0.5% n-3 HUFA (BFH, SOH, ROH, and LOH), resulting in a total of eight experimental diets. The control diet contained fish oil (FO) as the lipid source. Fish averaging $10.3{\pm}0.03g$ were fed the experimental diets in randomly selected triplicate groups for 8 weeks. Weight gain and feeding efficiency of fish fed the FO and SOH diets were significantly higher than those of fish fed BF or RO (P<0.05), but these diets did not differ significantly from the other diets. The protein efficiency ratio of fish fed the SOH diet was significantly higher than that of fish fed the BF, SO, or RO diets (P<0.05), but these were not significantly different from the other diets. The specific growth rate of fish fed the FO and SOH diets was significantly higher than that of fish fed the BF diet (P<0.05). Whole body DHA and n-3 HUFA contents of fish fed the FO diet were significantly higher than those of fish fed the SO, RO, or LO diets (P<0.05), but were not significantly different from the other diets. These results indicate that soybean oil and linseed oil could replace up to 100% of fish oil in the diet containing 60% fishmeal for river puffer fish.

• The Sea:JOURNAL OF THE KOREAN SOCIETY OF OCEANOGRAPHY
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• v.8 no.3
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• pp.237-242
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• 2003

Microstructure of otoliths and early growth in total length of laboratory-reared river puffer, Takrifugu obscurus were observed from 1 to 63 days after hatching. The total length of Takifugu obscurus was 3.09${\pm}$0.02 mm, 4.96${\pm}$0.24 mm, 5.39${\pm}$0.42 mm,9.13${\pm}$0.59 mm, 10.78${\pm}$0.96, 16.40${\pm}$2.31 mm, 30.49${\pm}$l.53 mm in 1, 10, 20, 30, 34, 43, 63 days respectively. The mean radius of otoliths was 14.20${\pm}$1.21 $\mu\textrm{m}$ in 1 day, 31.06${\pm}$2.31 $\mu\textrm{m}$ in 10 days,57.19${\pm}$6.19 $\mu\textrm{m}$ in 20 days,127.13${\pm}$13.13 $\mu\textrm{m}$ in 34 days,190.79${\pm}$17.08 $\mu\textrm{m}$ in 43 days and 281.85${\pm}$23.81 $\mu\textrm{m}$ in 63 days after hatching. Growth of otoliths were similar to the growth pattern in total length during 20-63 days after hatching. Hatch mark was distinguished with other growth increments and within a hatch mark two or three increments were observed. The number of growth increments was correlated with the days after hatching between 20 and 63 days after hatching.

• Development and Reproduction
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• v.15 no.2
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• pp.77-85
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• 2011

To investigate the influence of density and feeding frequency (food amount) on growth, cannibal dynamics and survival of puffer Takifugu obscurus (TL $25.0{\pm}0.5$ mm, BW $0.53{\pm}0.03$ g) during early life history, we conducted one experiment composed with 6 density groups set up as 1.43 inds./${\ell}$, 2.14 inds./${\ell}$, 2.86 inds./${\ell}$, 3.57 inds./${\ell}$, 4.29 inds./${\ell}$ and 7.14 inds./${\ell}$. The experiment was triplicately performed in 1 ton FRP tank for 30 days. Daily food intake (DFI), feed efficiency (FE), growth, cannibal frequency, and survival rates were examined. Although not find any significant relation of density to DFI and FE, we found that growth decreased significantly with the increasing of density under 3.57 inds./${\ell}$, but significantly increased with the increasing of density over 4.29 inds./${\ell}$. Cannibal frequency and daily mortality rate was significantly increased with increasing density and decreasing feeding frequency, inducing the most poor survival rate in the highest density group and low feeding frequency. Therefore, we concluded that the density and the feeding frequencies affect sibling cannibal dynamics and survival of puffer stock.

• Journal of Aquaculture
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• v.17 no.3
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• pp.221-227
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• 2004

In the present study, we investigated the effects of food and salinity on growth and survival of river puffer, Takifugu obscurus offsprings in indoor land-based tank during the early development. In the food experiment, the river puffer larvae (TL 8.7$\pm$0.1 mm, BW 20.0$\pm$3.2 mg) were fed with tubificid, Limnodrilus gotoi, water flea, Daphnia carinata, mysid, Neomysis awatschensis, Manila clam, Ruditapes philippinarum and artificial food together Artemia nauplii for 30 days, and the growth and the survival of larvae were investigated. In the salinity experiment, the river puffer larvae and juvenile at three stages (Stage I: TL 5.8$\pm$0.4 mm; Stage II: TL 12.0$\pm$0.2 mm, Stage III： TL 44.5$\pm$0.7 mm) were supplied with Artemia nauplii, water flea and/or artificial food and were reared in 0, 10, 20 and 30 psu at respective stages. The results shows that the growth rates of larvae feeding with artificial food and tubificid together Artemia nauplii were higher than those of larvae in other groups, but the survival rates of larvae feeding with water flea and mysid together Artemia nauplii were higher than those of larvae in other groups. In salinity, the results shows that the growth and survival rates of offsprings were significantly higher in 10∼20 psu than those in 0 and 30 psu at Stage I and n. At Stage III, the growth and the survival rates were the highest in 20 psu, but the lowest in 0 psu. However, it should be noted that the rates in 0 and 30 psu were significantly enhanced at Stage III in comparison with those at Stage I and II. Therefore, it is concluded that river puffer, T. obscurus is euryhaline marine species, which can normally grow and live at salinities from 0 to 30 psu, and that a combinative supplement with Artemia nauplii and water flea, D. carinata may confer an advantage on growth and survival of the river puffer offsprings in indoor land-based tank.

• Korean Journal of Ichthyology
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• v.5 no.1
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• pp.41-52
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• 1993

During the investigation of fish fauna from the Kokunsan Islands off west coast of Korea from 1989 to 1993, authors confirmed 102 fish species belonging to 81 genera, 50 families and 12 orders. Of these the following 8 species were common : Herengila zunasi, Engraulis japonica, Johnius grypotus, Pholis fangi, Repomucenus ornatipinnis, Chasmichthys gulosus, Tridentiger trigonocephalus, and Cynoglossus joyneri. Of the twelve orders recognized herein, Perciformes, Scorpaeniformes and Pleuronectiformes include approximately 74% of the fish fauna in this area. The largest family is the Gobiidae representing 12 species. Pholis fangi, Repomucenus ornatipinnis, Repomucenus koreanus, Takifugu flavidus and Takifugu obscurus collected are endemic to the Yellow Sea. Fifteen specimens of an unknown Sebastes species was collected. This species resembles Sebastes hubbsi and Sebastes longispinnis, but the species differs from other sebastes species reported in Korea in the number of dorsal fin rays, lateral line pores, gill rakers and in color patterns of body sides.

• Proceedings of the Korean Aquaculture Society Conference
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• 2004.05a
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• pp.284-285
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• 2004

• Journal of fish pathology
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• v.17 no.2
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• pp.99-103
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• 2004

White spot disease by Cryptocaryon irritans broke out among cultured river puffer (Takifugu obscurus) cultured in sea water in Oct. 2001 and May 2004. Many white spots were easily observed on the gills with the naked eyes. The parasites attached on the gills at the base of the gill lamellae. The trophonts penetrated the interlamellar epithelium. Epithelial cell proliferation in interlamellar spaces occurred. PAS- positive mucous cells were prominent in the proliferated epithelium.

• Development and Reproduction
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• v.23 no.3
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• pp.239-253
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• 2019

We investigated the physiological responses of the river puffer, Takifugu obscurus, the tiger puffer, T. rubripes, a hybrids of the two (river puffer${\times}$tiger puffer) and a hybrid triploids to acute changes of salinity from 30 psu to 0 psu and from 0 psu to 30 psu. The blood and plasma factors of each species were elevated for 48, 72, or 96 hrs, and thereafter decreased due to hyper-osmoregulation and hypo-osmoregulation. In hyper-osmoregulation and hypo-osmoregulation, the cortisol concentration of river puffer, hybrids, hybrid triploids and tiger puffer increased for 12 or 48 hrs, and decreased thereafter. Chloride cells in the gill filaments of each species increased with increasing salinity, and melano-macrophages in the kidney tissue of each species increased with decreasing salinity. In conclusion, the hematological and stress responses of the hybrids were between those of the river puffer and tiger puffer, and the hematological responses of the hybrid triploids were higher than those of the other groups. The stress response of the hybrids was more sensitive than that of the hybrid triploids. In all groups, the histological responses of kidney in hyper-osmoregulation were more sensitive than those in hypo-osmoregulation.

• Development and Reproduction
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• v.24 no.4
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• pp.327-335
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• 2020

Genomic DNA (gDNA) extracted from two populations of natural and cultured river pufferfish (Takifugu obscurus) was amplified by polymerase chain reaction (PCR). The complexity of the fragments derived from the two locations varied dramatically. The genetic distances (GDs) between individuals numbered 15 and 12 in the cultured population was 0.053, which was the lowest acknowledged. The oligonucleotide primer OPC-11 identified 88 unique loci shared within each population reflecting the natural population. The OPC-05 primer identified 44 loci shared by the two populations. The average band-sharing (BS) values of individuals in the natural population (0.683±0.014) were lower than in those derived from the cultured population (0.759±0.009) (p<0.05). The shortest GD demonstrating a significant molecular difference was found between the cultured individuals # 15 and # 12 (GD=0.053). Individual # 02 of the natural population was most distantly related to cultured individual # 22 (GD=0.827). A cluster tree was built using the unweighted pair group method with arithmetic mean (UPGMA) Euclidean GD analysis based on a total of 578 various fragments derived from five primers in the two populations. Obvious markers identified in this study represent the genetic structure, species security, and proliferation of river pufferfish in the rivers of the Korean peninsula.