• BMB Reports
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• 제53권8호
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• pp.425-430
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• 2020

Tumor necrosis factor-inducible gene 6 protein (TSG-6) is a cytokine secreted by mesenchymal stem cells (MSCs) and regulates MSC stemness. We previously reported that TSG-6 changes primary human hepatic stellate cells (pHSCs) into stem-like cells by activating yes-associated protein-1 (YAP-1). However, the molecular mechanism behind the reprogramming action of TSG-6 in pHSCs remains unknown. Cluster of differentiation 44 (CD44) is a transmembrane protein that has multiple functions depending on the ligand it is binding, and it is involved in various signaling pathways, including the Wnt/β-catenin pathway. Given that β-catenin influences stemness and acts downstream of CD44, we hypothesized that TSG-6 interacts with the CD44 receptor and stimulates β-catenin to activate YAP-1 during TSG-6-mediated transdifferentiation of HSCs. Immunoprecipitation assays showed the interaction of TSG-6 with CD44, and immunofluorescence staining analyses revealed the colocalization of TSG-6 and CD44 at the plasma membrane of TSG-6-treated pHSCs. In addition, TSG-6 treatment upregulated the inactive form of phosphorylated glycogen synthase kinase (GSK)-3β, which is a negative regulator of β-catenin, and promoted nuclear accumulation of active/nonphosphorylated β-catenin, eventually leading to the activation of YAP-1. However, CD44 suppression in pHSCs following CD44 siRNA treatment blocked the activation of β-catenin and YAP-1, which inhibited the transition of TSG-6-treated HSCs into stem-like cells. Therefore, these findings demonstrate that TSG-6 interacts with CD44 and activates β-catenin and YAP-1 during the conversion of TSG-6-treated pHSCs into stem-like cells, suggesting that this novel pathway is an effective therapeutic target for controlling liver disease.

• The Korean Journal of Physiology and Pharmacology
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• 제24권6호
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• pp.473-479
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• 2020

Endothelial cell injury is a major contributor to cardiovascular diseases. The 2,3,5,4'-Tetrahydroxystilbene-2-O-β-D-Glucoside (TSG) contributes to alleviate human umbilical vein endothelial cells (HUVECs) injury through mechanisms still know a little. This study aims to clarify the TSG effects on gene expression (mRNA and microRNA) related to oxidative stress and endoplasmic reticulum stress induced by H₂O₂ in HUVECs. We found that TSG significantly reduced the death rate of cells and increased intracellular superoxide dismutase activity. At qRT-PCR, experimental data showed that TSG significantly counteracted the expressions of miR-9-5p, miR-16, miR-21, miR-29b, miR-145-5p, and miR-204-5p. Besides, TSG prevented the expression of ATF6 and CHOP increasing. In contrast, TSG promoted the expression of E2F1. In conclusion, our results point to the obvious protective effect of TSG on HUVECs injury induced by H₂O₂, and the mechanism may through miR16/ATF6/ E2F1 signaling pathway.

• Ocean and Polar Research
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• 제24권3호
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• pp.207-213
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• 2002

We have analyzed sea surface temperature and salinity data collected in the western Pacific Ocean by using an automated Thermosalinograph (TSG) installed on the RV Onnuri during May - June of 2001. The TSG data exhibit characteristics of water masses distributed in the near surface layer of the cruise area very well. Especially, they reveal the diurnal surface temperature cycle and the effect of rainfall on temperature and salinity in the equatorial region, showing the effectiveness of the use of TSG. Problems to be improved for the better TSG operation are the method of water sampling and calibration of TSG sensors. Installation of a pressure gauge in the TSG system and periodical sensor calibration are strongly recommended to ensure reliability of data.

• 전자통신동향분석
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• 제16권6호통권72호
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• pp.126-132
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• 2001

WCDMA 비동기 방식의 IMT-2000 규격 정립을 위해, 3GPP의 여러 실무 그룹에서 Release ’99, Release ’4 및 Release ’5에 대한 표준화가 동시에 이루어지고 있다. 본 고에서는 UTRA 프로토콜의 계층 2와 3 표준화를 담당하고 있는 3GPP TSG RAN WG 2의 규격의 표준화 현황 및 새로이 규격에 반영될 기술들에 대해 살펴본다.

• TTA 저널
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• 통권160호
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• pp.109-111
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• 2015

• TTA 저널
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• 통권163호
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• pp.102-104
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• 2016

• TTA 저널
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• 통권164호
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• pp.96-100
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• 2016

• 정보보호학회논문지
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• 제11권6호
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• pp.53-65
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• 2001

W-CDMA 방식 IMT-2000 시스템에 대한 인증 메커니즘은 3GPP TSG SA WG3에 의해서 개발되었고, 이에 따라 우리는 인증 메커너즘과 알고리즘을 GUI 환경에서 시뮬레이션 하였다. 본 논문에서는, 3GPP의 인증 절차를 분석하고, GUI 환경에서의 시뮬레이션에 관해서 설명한다. 또한 메커니즘의 타당성을 검증하고, 전송되는 보안 파라미터(security parameter) 중 스펙에서 명확하게 언급되지 않은 부분에 관해서 논의한다.

• Journal of Periodontal and Implant Science
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• 제53권5호
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• pp.347-361
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• 2023

Purpose: Exosomes are membrane vesicles that are present in body fluids and contain proteins, lipids, and microRNA (miRNA). Periodontal tissue examinations assess the degree of periodontal tissue destruction according to the probing depth (PD), clinical attachment loss (CAL), bleeding on probing, and X-ray examinations. However, the accurate evaluation of the prognosis of periodontitis is limited. In this study, we collected saliva from patients before and after initial periodontal therapy (IPT) and compared changes in the clinical parameters of periodontitis with changes in the components of salivary exosomes. Methods: Saliva was collected from patients with stage III and IV periodontitis at the first visit and post-IPT. Exosomes were purified from the saliva, and total protein and RNA were extracted. Changes in expression levels of C6, CD81, TSG101, HSP70, and 6 kinds of miRNA were analyzed by western blots and real-time polymerase chain reaction. Results: Patients with increased C6 expression after IPT had significantly higher levels of periodontal inflamed surface area (PISA), miR-142, and miR-144 before and after IPT than patients with decreased C6 expression after IPT. Patients with decreased and unchanged CD81 expression after IPT showed significantly higher PD, CAL, and PISA before IPT than after IPT. Patients with decreased and unchanged TSG101 expression after IPT had significantly higher PD before IPT than after IPT. Patients with increased HSP70 expression after IPT had significantly higher PD and PISA before and after IPT than patients with unchanged HSP70 after IPT. The expression levels of miR-142, miR-144, miR-200b, and miR-223 changed with changes in the levels of C6, CD81, TSG101, and HSP70 in the salivary exosomes of periodontitis patients before and after IPT. Conclusions: The expression levels of proteins and miRNAs in salivary exosomes significantly changed after IPT in periodontitis patients, suggesting that the components of exosomes could serve as biomarkers for periodontitis.

• 정보보호학회지
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• 제11권6호
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• pp.63-73
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• 2001

3세대 방식인 IMT-2000 (International Mobile Telecommunication-2000)시스템은 GSM이나 IS-95 CDMA 시스템과 같은 2세대 이동통신 시스템과 비교하여 고속의 멀티미디어 서비스 제공 및 글로벌 로밍을 특징으로 한다. W-CDMA 방식 IMT-2000에 대한 인증 메커니즘은 3GPP TSG SA WG3에 의해서 개발되었다. 본 논문에서는 3GPP에서의 AKA(Authentication & Key Agreement)를 분석하고 이를 통해 안전성 분석 및 취약성을 분석한다. 본 논문에서 제기하는 안전성 분석 및 취약성 분석은 앞으로의 더 좋은 서비스를 제공할 것이다.