• Proceedings of the KIEE Conference
• /
• 1988.11a
• /
• pp.236-240
• /
• 1988

The simplified equivalent by using the short circuit impedance has been used for analyzing the prospective transient recovery voltage of the large power system. But it sometimes generates untorelable error in the rate-of-rise of TRV when using the Thevenin equivalent source. This paper provides the new equivalent by using the multi-port theory. The application of the new method to the sample system gives satisfactory accuracy compared with the short-circuit equivalent.

• 전기의세계
• /
• v.29 no.6
• /
• pp.354-361
• /
• 1980

Weil-Dobke 회로 및 2-parameter TRV회로의 문제점을 검토하고 비교적 간단한 네가지의 4-parameter TRV 회로에 대한 기초적인 검토가 이루어졌으나 현단계에서 각 회로에 대한 회로계산등의 적극적인 검토를 할 수는 없었고 앞으로 컴퓨터나 TNA등의 설비를 이용할 수 있을 경우 전반적인 비교검토를 하고 그것을 바탕으로 우리 연구소의 기존 설비를 최대로 활용할 수 있는 회로를 채택하는 것이 바람직하다.

• Proceedings of the KIEE Conference
• /
• 2005.07a
• /
• pp.368-370
• /
• 2005

Due to the tendency towards large capacity and complexity of power system, an enhancement of power system equipment make a system impedance to be low in power system. Generally if an equivalent impedance of system becomes lower, a system stability will be better. But the fault current becomes very larger. The 345kV ultra-high voltage system will use current limit reactors(CLR) in a transmission line or a bus in substation to limit the magnitude of fault current. The CLR makes a significant contribution to the severity of the transient recovery voltage(TRV) experienced by feeder and bus circuit breakers on clearing feeder faults. Based on the conclusions of an investigation of actual circuit breaker failures while performing this duty, the mitigation of the transient recovery voltage associated with the reactors is described. Therefore in this article we simulated the TRV by EMTP at Bus Terminal Fault.

• Korean Journal of Agricultural Science
• /
• v.42 no.1
• /
• pp.7-13
• /
• 2015

Ninety genes randomly selected from tobacco whitefly (Bemisia tabaci) cDNA library was studied for selecting target gene in order to control of tobacco whitefly using TRV-VIGS vector (tobacco rattle virus-virus induced gene silencing vector) with RNAi. First of all, the occurrence of B. tabaci adult according to agro-infiltration of TRV was no significant difference. And that of TRV inserted tobacco whitefly cDNA showed a significant difference in each sample. P CV and N CV sample were more than 80% could be confirmed in 5 samples, for example, wh11, wh36, wh46, wh50 and wh71. Lastly, the occurrence of nymph and egg also showed a significant difference in each sample. That could be confirmed in 11 samples, for example, wh01, wh09, wh10, wh15, wh16, wh23, wh24, wh48, wh64 and wh66. In case of wh46, wh50 and wh71 sample could be confirmed that occurrence of B. tabaci adult was many, but occurrence of B. tabaci nymph and egg was a little. So sample showed a physioecological good effect to control of whitefly need to be investigated variation of gene expression in whitefly body using qRT-PCR through individual test.

• Molecules and Cells
• /
• v.44 no.12
• /
• pp.911-919
• /
• 2021

The virus-induced genome editing (VIGE) system aims to induce targeted mutations in seeds without requiring any tissue culture. Here, we show that tobacco rattle virus (TRV) harboring guide RNA (gRNA) edits germ cells in a wild tobacco, Nicotiana attenuata, that expresses Streptococcus pyogenes Cas9 (SpCas9). We first generated N. attenuata transgenic plants expressing SpCas9 under the control of 35S promoter and infected rosette leaves with TRV carrying gRNA. Gene-edited seeds were not found in the progeny of the infected N. attenuata. Next, the N. attenuata ribosomal protein S5 A (RPS5A) promoter fused to SpCas9 was employed to induce the heritable gene editing with TRV. The RPS5A promoter-driven SpCas9 successfully produced monoallelic mutations at three target genes in N. attenuata seeds with TRV-delivered guide RNA. These monoallelic mutations were found in 2%-6% seeds among M₁ progenies. This editing method provides an alternative way to increase the heritable editing efficacy of VIGE.

• The Plant Pathology Journal
• /
• v.21 no.2
• /
• pp.158-163
• /
• 2005

Virus-induced gene silencing (VIGS) is a recently developed gene transcript suppression technique for characterizing the function of plant genes. However, efficient VIGS has only been studied in a few plant species. In order to extend the application of VIGS, we examined whether a VIGS vector based on TRV would produce recognizable phenotypes in soybean. Here, we report that VIGS using the Tobacco rattle virus (TRV) viral vector can be used in several soybean cultivars employing various agro-inoculation methods including leaf infiltration, spray inoculation, and agrodrench. cDNA fragments of the soybean phytoene desaturase(PDS) was inserted into TRV RNA-2 vector. By agrodrench, we successfully silenced the expression of PDS encoding gene in soybean. The silenced phenotype of PDS was invariably obvious 3 weeks after inoculation with the TRV-based vector. Real-time RT-PCR analyses showed that the endogenous level of GmPDS transcripts was dramatically reduced in the silenced leaf tissues. These observations confirm that the silenced phenotype is closely correlated with the pattern of tissue expression. The TRV-based VIGS using agrodrench can be applied to functional genomics in a soybean plants to study genes involved in a wide range of biological processes. To our knowledge, this is the first high frequency VIGS method in soybean plants.

• Proceedings of the KIEE Conference
• /
• 2007.10c
• /
• pp.91-92
• /
• 2007

저압 개폐기는 에어컨, 냉동기, 산업용 청소기, 전기온수기, 공조기, 펌프, 콤프레션 등의 모터제어 및 히터와 조명기기의 개폐 둥에 광범위하게 이용되는 개폐장치이다. 저압 전자개폐기는 수 천회에서 수백만회까지 빈번한 개폐 사용조건으로 인해 시험 규격에서는 개폐시험을 통해 제품의 수명을 검증하도록 되어 있으며 과다한 시간이 소요된다. 따라서 시험 시간을 단축하기 위한 가속수명 시험이 필요하게 되었으며 가속 스트레스인 과도 회복전압(TRV)의 피크비율(factor ${\gamma}$) 변화에 따라 저압개폐기의 전기적 수명 시험을 실시하였으며 시험결과를 바탕으로 가속 수명 시험을 하기 위한 가속계수를 산출하였다.

• Proceedings of the KIEE Conference
• /
• 1998.11c
• /
• pp.962-965
• /
• 1998

The circuit breakers had been withdrawn from 345 kV SIC substation. because these were made following the old IEC requirement(IEC 56; TRV rating) and insufficient current rating(2,000 A) for the system requirement(4,000 A). And, they are going to be reused at 345 kV UR substation and SJS switching section. This paper deals with the TRV(temporary recovery voltage) analysis results in order to reuse them. The analysis results are satisfied with the IEC requirements of RRRV(Rate of Rise of Recovery Voltage) and peak voltage($U_c$) from 2000 to 2010.

• Korean Journal of Agricultural Science
• /
• v.42 no.2
• /
• pp.81-86
• /
• 2015

Three genes selected from cDNA library of tobacco whitefly, Bemisia tabaci, were checked whether these genes expressed in plant or not, and confirmed the change of gene expression using qRT-PCR in the tobacco whitefly. First of all, three genes were inserted in Tobacco rattle virus (TRV) RNA2 vector using Sac I and Xho I restriction enzymes, and conducted agro-infiltration in tobacco plants (Nicotiana benthamianana). And then, it was confirmed that TRV RNA2 vector and genes inserted in TRV RNA2 vector were expressed in plant. So, after feeding the tobacco whitefly the plants inoculated the genes and induced RNAi of the genes, we plan to confirm the RNAi in the whitefly and investigate the changes of gene expression through the qRT-PCR.

• Proceedings of the Korean Society for Agricultural Machinery Conference
• /
• 1999.12a
• /
• pp.661-667
• /
• 1999