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Effect of Feeding Ammoniated Wheat Straw Treated with Hydrochloric Acid on Blood Biochemical Profile in Growing Male Buffalo (Bubalus bubalis) Calves

  • Naik, P.K.;Mehra, Usha R.;Kalicharan, Kalicharan;Varshney, V.P.;Dass, R.S.
    • Asian-Australasian Journal of Animal Sciences
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    • v.18 no.2
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    • pp.185-191
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    • 2005
  • An experiment was conducted to study the effect of feeding ammoniated wheat straw treated with HCl on blood biochemical profiles in growing male buffalo (Bubalus bubalis) calves. Twenty-four growing male buffalo calves (one year of age, 88.54${\pm}$3.81 kg average body weight) were divided into three groups in a completely randomized design on the basis of their body weight. Animals in all the three groups were fed on concentrate mixture. In addition, they were offered wheat straw, ammoniated wheat straw (4% urea at 50% moisture level) and HCI treated ammoniated wheat straw (4% urea at 50% moisture level and HCl added to trap 30% of the NH3 evolved) in groups I, II and III, respectively for a period of 180 days, as per Kearl (1982) for body weight gain of 500 g/d. In all diets, concentrate:roughage ratio was fixed at 50:50 and were made isonitrogenous by adjusting CP levels of conc. mixtures. Blood was collected from jugular vein of each buffalo calf at the beginning and subsequently at two months interval of experimental feeding. Due to urea-ammoniation, the CP content of wheat straw increased from 2.90 to 6.96% and addition of HCl along with urea further increased the CP content to 10.09%. In all the three groups, the mean values of plasma glucose (mg %) and serum globulin (g %), showed a decreasing trend, while the mean value of serum TP (g %), serum A:G ratio, serum urea (mg %), serum creatinine (mg %), serum ALP (KA units), SGOT (units/ml.), SGPT (units/ml), serum T$_3$ and T$_4$ (ng/ml) showed an increasing trend with the advancement of feeding period. The cumulative period mean values of serum TP (6.15 to 6.20 g %), serum albumin (3.07 to 3.18, g %), serum globulin (2.98 to 3.09, g %), serum A:G ratio (1.03 to 1.10), serum ALP (23.15 to 23.63, KA units), serum T$_3$ (1.20 to 1.23 ng/ml) and serum T$_4$ (21.33 to 21.88 ng/ml) were comparable among the groups. The cumulative period mean plasma glucose (mg %) in group III (57.28) was similar to groups I (55.31) and II (59.41), however, the cumulative period mean plasma glucose in group II was significantly (p<0.01) higher than group I. The cumulative period mean serum urea (mg %) in group III (47.34) was significantly (p<0.001) higher than group I (38.38) and II (42.24), which were statistically alike. However, the cumulative period mean serum creatinine values (mg %) in groups II (1.43) and III (1.52) were similar and were significantly (p<0.01) higher than group I (1.24). The cumulative period mean SGOT (units/ml) in groups I, II and III was 91.71, 96.04 and 96.64, respectively. Similarly the cumulative period mean SGPT (units/ml) was 19.00, 19.93 and 20.01 in groups I, II and III, respectively. The cumulative period mean values of SGOT (p<0.05) and SGPT (p<0.001) in groups II and III were similar and were significantly higher than group I. The cumulative period mean serum T$_3$ and T$_4$ values in groups I (1.21 and 21.81), II (1.23 and 21.42) and III (1.20 and 21.33) were comparable. From the present study it may be concluded that feeding of AWS treated with and without HCI to growing male buffalo calves for 180 days had no significant adverse effect on blood biochemical profile.

Tectonic Structures and Hydrocarbon Potential in the Central Bransfield Basin, Antarctica (남극 브랜스필드 해협 중앙분지의 지체구조 및 석유부존 가능성)

  • Huh Sik;Kim Yeadong;Cheong Dae-Kyo;Jin Young Keun;Nam Sang Heon
    • The Korean Journal of Petroleum Geology
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    • v.5 no.1_2 s.6
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    • pp.9-15
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    • 1997
  • The study area is located in the Central Bransfield Basin, Antarctica. To analyze the morphology of seafloor, structure of basement, and seismic stratigraphy of the sedimentary layers, we have acquired, processed, and interpreted the multi-channel seismic data. The northwest-southeastern back-arc extension dramatically changes seafloor morphology, volcanic and fault distribution, and basin structure along the spreading ridges. The northern continental shelf shows a narrow, steep topography. In contrast, the continental shelf or slope in the south, which is connected to the Antarctic Peninsula, has a gentle gradient. Volcanic activities resulted in the formation of large volcanos and basement highs near the spreading center, and small-scale volcanic diapirs on the shelf. A very long, continuous normal fault characterizes the northern shelf, whereas several basinward synthetic faults probably detach into the master fault in the south. Four transfer faults, the northwest-southeastern deep-parallel structures, controlled the complex distributions of the volcanos, normal faults, depocenters, and possibly hydrocarbon provinces in the study area. They have also deformed the basement structure and depositional pattern. Even though the Bransfield Basin was believed to be formed in the Late Cenozoic (about 4 Ma), the hydrocarbon potential may be very high due to thick sediment accumulation, high organic contents, high heat flow resulted from the active tectonics, and adequate traps.

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Sedimentary History and Tectonics in the Southeastern Continental Shelf of Korea based on High Resolution Shallow Seismic Data. (고해상탄성파탐사자료에 의한 한국남동대륙붕의 퇴적사 및 조구조운동)

  • Min Geon Hong;Park Yong Ahn
    • The Korean Journal of Petroleum Geology
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    • v.5 no.1_2 s.6
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    • pp.1-8
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    • 1997
  • Seismic stratigraphic analysis of the high resolution profiles obtained from the southeastern shelf of Korea divided the deposits into 4 sequences; 1) sequence D, 2) sequence C, 3) sequence B and 4) sequence A (Holocene sediments). Sequence D was deposited in shallow-water environment at west of the Yangsan Fault as the basin subsided. On the other hand, the eastern part was formed at the slope front. Landward part of the slope-front fill sediments were eroded and redeposited nearby slope due to the syndepositional tilting of the basin. This tilting probably resulted from the continuous closing of the Ulleung Basin. Sequence C is made of stacked successions of the lowstand fluvial sediments, transgressive sediments and marine highstand sediments derived from the paleo-river in the western part of the Yangsan Fault. Sequence C in the eastern part of the Yanshan Fault was formed at the shelf break. Progradation of the lowstand sediments resulted in broadening of the shelf. Sequence C in the eastern part was also tilted but the tilting was weaker than in Sequence D. During the formation of sequence B the tilting stopped and the point source instead of the line source started in both sides of the Yangsan Fault. Sequence B was composed of the highstand systems tract partially preserved around the Yokji island, lowstand systems tract mainly preserved in the Korea Trough and transgressive systems tract. After the stop of the tilting, the force of compression due to the closing of the Ulleung Basin may be released by the strike-slip faults instead of tilting.

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Flowering Characteristics and Acorn Production of Quercus auta Thunb. in Wando Island, Korea (완도지역 붉가시나무의 개화특성과 종실생산)

  • Park, In-Hyeop;Kim, So-Dam;Park, Jong-Won;Chae, Kyung-Seog;Kim, Gab-Tae;Um, Tae-Won
    • Korean Journal of Environment and Ecology
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    • v.28 no.3
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    • pp.326-330
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    • 2014
  • Flowering characteristics and acorn production of Quercus acuta were studied in a Quercus acuta forest at Wando island, Korea. Three sample trees were cut and measured to investigate flowering characteristics during flowering season. Eight $20m{\times}25m$ quadrats were set up and three $1m{\times}1m$ seed traps were established within each quadrat to investigate acorn production. There were no significant differences in number of female and male inflorescence per bearing shoot among crown positions, but these values decreased from the upper part to the lower part of crown. The number of female flowers was 5,025 flowers/tree, and of the total number, 60.3% was in the upper part, 28.4% was in the middle part and 11.3% was in the lower part of crown. The number of male flowers was 7,063 flowers/tree and increased from the lower part to the upper part of crown. Acorn production of total 24 seed traps was 947 acorns/$24m^2$, and of total acorn production, 11.0% was sound, 61.9% was damaged, 25.1% was decayed and 2.0% was empty. Monthly acorn production was 240 acorns/$24m^2$ in August, 632 acorns/$24m^2$ in September, 56 acorns/$24m^2$ in October, 17 acorns/$24m^2$ in November and 2 acorns/$24m^2$ in December. Acorns fallen in October showed higher mean length, diameter and weight than those of other months.

Diagnostic assessment on vegetation damage due to hydrofluoric gas leak accident and restoration planning to mitigate the damage in a forest ecosystem around Hube Globe in Gumi (구미 휴브글로브 주변 삼림생태계에서 불화수소가스 유출 사고에 기인한 식생피해 진단 및 그 피해를 완화시키기 위한 복원 계획)

  • Kim, Gyung Soon;An, Ji Hong;Lim, Chi Hong;Lim, Yun Kyung;Jung, Song Hie;Lee, Chang Seok
    • Journal of Wetlands Research
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    • v.17 no.1
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    • pp.45-52
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    • 2015
  • We obtained the following results from investigation on vegetation damage from 5 to 6 August, 2013, about one year after an accident that hydrofluoric acid leaked from a chemical maker, Hube Globe in Gumi. Pinus densiflora and Pinus strobus showed very severe damage. Ginko biloba, Quercus acutissima, Pinus rigida, Salix glandulosa, Hibiscus syriacus, and Lagerstroemia indica showed severe damage. Quercus variabilis, Lespedeza cyrtobotrya, and Miscanthus sinensis showed moderate damage. Quercus aliena, Smilax china, Arundidinella hirta, Ailanthus altissima, Robinia pseudoacacia, and Paulowinia coreana showed slight damage. We did not find any plants without leaf damage around there. This result means that fluoride damage still persists in this area as was known that fluoride remains for a long time in air, soil and water and exerts negative effects at all levels of an ecosystem. In addition, fluoride content contained in plant leaf depended on the distance from a fertilizer producing factory and vegetation damage tended to proportionate to the concentration in the Yeocheon industrial complex. In these respects, a measure for removal or detoxification of the remaining fluoride is urgently required around the hydrofluoric acid leak spot. Fertilizing of dolomite containing Ca and Mg, which can trap fluoride, was prepared as one of the restoration plans. In addition, phosphate fertilizing was added in order to enhance soil ameliorating effects. Furthermore, we recommend the introduction of tolerant plants as the second measure to mitigate fluoride damage. As the tolerant plants to make a new forest by replacing trees died due to hydrofluoric acid gas damage, we recommended Q. aliena and S. china, A. hirta, etc. were recommended as plant species to add mantle vegetation to the forest margin to ensure stable interior environment of the forest.

Seasonal Occurrence of Tobacco Cutworm, Spodoptera litura Fabricius and Beet Armyworm, Spodoptera exigua Hübner Using Sex Pheromone Traps at Different Locations and Regions in Yeongnam District (영남지방 지역 및 지대별 담배거세미나방과 파밤나방 성충의 발생소장)

  • Bae, Soon-Do;Kim, Hyun-Ju;Lee, Geon-Hwi;Park, Sung-Tae
    • Korean journal of applied entomology
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    • v.46 no.1 s.145
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    • pp.27-35
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    • 2007
  • Seasonal occurrences of tobacco cutworm, Spodoptera litura and beet armyworm, Spodoptera exigua were observed using sex pheromone traps at different locations and climatic regions in Yeongnam district. In Gyeongnam province, S. litura male adults kept occur from mid or late March to mid or late November Whereas in Gyeongbuk province, S. litura occurred from mid or late April to early or mid November with exception at Uljin where it was late May to late October. According to the climatic regions, the peak occurrence of S. litura was middle September at the southern coast region, late August at the inland and the basin region, middle August at the inland mountainous region, and early September at the eastern middle coast region. The occurrence rate of S. litura was the highest at Jinju with 20.0% and the lowest at Changyeong with 8.6% in Cyeongnam province, while it was the highest at Sungju with 3.3% and the lowest at Uljin with 0.8% in Gyeongbuk province. In accordance with the climatic regions, the occurrence rate of S. litura was the highest at the inland region with 43.7%, and followed by the southern coast region (36.3%), the basin region (8.4%) and the inland mountainous region (7.0%), and was the lowest at the eastern middle coast legion with 4.6%. On the other hand, S. exigua occurred from early March to middle November with the exception of the eastern middle coast region including Pohang, Yeongdeog and U]ien in Cyeongbuk province where it occurred from mfd April to middle November. The peak occurrence of s. exigua was early August at the inland region, the basin region and the inland mountainous region, and was late of August at the southern coast region and the eastern middle coast region. The occurrence of S. exigua was the highest at Changyeong with 1.6% and the lowest at Busan with 0.4% in Gyeongnam province, while it was the highest at Sungju with 37.2% and the lowest at Uljin with 2.7% in Gyeongbuk province. The climatic regional occurrence rate of S. exigua was the highest at the basin region with 54.3%, and followed by the inland mountainous region (29.9%), the eastern middle coast region (9.3%) and the inland (3.9%), and was the lowest at the southern coast region with 2.6%. As a whole, S. litura occurred more in Gyeongnam province than Cyeongbuk province, while S. exigua occurred more in Gyeongbuk province than Gyeongnam province. According to the regions grouped by climatic differences, the occurrence of S. litura and S. exigua was the highest at the inland region in Gyeongnam province and at the basin region in Gyeongbuk province. The total number of S. litura captured by sex pheromone trap was 2.4 times higher than that of S. exigua. However, the first occurring time and the peak occurrence of S. exigua were slightly earlier than those of S. litura.

Inhibitory Effects of Functional Sujeonggwa (Cinnamon Drink) on Lipid Peroxidation and DNA Damage in Diet-Induced Hypercholesterolemic ApoE Knockout Mice (고콜레스테롤혈증 ApoE Knockout 마우스에서 기능성 수정과의 지질과산화 및 산화적 DNA 손상 억제 효과)

  • Park, Eunju;Baek, Aran;Kim, Mijeong;Lee, Seon Woo;Lee, Eunji;Choi, Mi-Joo;Lee, Jeehyun;Song, Yeong Ok
    • Journal of the Korean Society of Food Science and Nutrition
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    • v.43 no.11
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    • pp.1627-1634
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    • 2014
  • The recipe for sujeonggwa, a Korean traditional sweet drink containing cinnamon, ginger, sugar, or honey, was modified by replacing sugar with alternative sweeteners [stevia or short-chain frutooligosaccharide (scFOS)] in order to improve the health functionality of sujeonggwa. The aim of this study was to evaluate the effects of modified sujeonggwa on lipid peroxidation and oxidized DNA damage in diet-induced hypercholesterolemic ApoE knockout mice. Hypercholesterolemia was induced in 6-week-old male mice by administration of a high cholesterol diet (1.25% cholesterol, 0.5% cholic acid, and 10% coconut oil) for 4 weeks, after which mice were divided into five groups: sucrose solution-fed control group, sujeonggwa containing sucrose group, sucrose+stevia group, sucrose+stevia+scFOS group, and commercially available sujeonggwa group as a positive control. After 6 weeks, sujeonggwa supplementation resulted in reduced hepatic thiobarbituric acid reactive substances (TBARS), regardless of sweetener type. However, reduction of hepatic TBARS by commercially available sujeonggwa was insignificant. Both endogenous and $H_2O_2$-induced DNA damage in hepatocytes and splenocytes were significantly reduced only in the sujeonggwa containing stevia group compared to the sucrose-fed control group. There were no significant effects of sujeonggwa supplementation on total radical trapping potential, lipid peroxidation, or DNA damage in blood. These results suggest that sujeonggwa has protective effects against hepatic lipid peroxidation and DNA damage in hepatocytes or splenocytes from diet-induced hypercholesterolemic ApoE knockout mice, and the type of sweetener should be modified to improve the health benefits of sujeonggwa.

ESR-Spin Trapping Detection of Radical Center Formed on the Reaction of Metmyoglobin with Hydrogen Peroxide

  • Jeong, Sang-Hyeon;Hong, Sun-Joo
    • BMB Reports
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    • v.28 no.4
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    • pp.293-300
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    • 1995
  • The radical centers detected in the reaction of metmyoglobin (MetMb) with hydrogen peroxide ($H_2O_2$) have been studied by using a spin trapping technique. A broad 5-line asymmetric electron spin resonance (ESR) spectrum, with $2A_{max}=4.07\;mT$ and $2A_{min}=2.97\;mT$, obtained after incubation of MetMb with $H_2O_2$ in the presence of a spin trap, 5,5-dimethyl pyrroline-N-oxide (DMPO) was gradually weakened with time and disappeared completely by 6 min after addition of guanidine-HCl (14 M). When a higher concentration (6 M) of the agent was added, the signal disappeared within 40 see and the DMPO/OH signal appeared immediately. Then, a new 8-line signal with similar intensities grew gradually and was fixed by 45 min, coexisting with the DMPO/OH signal. This new signal was found to be composite, consisting of two different radical species. One of the 6-line signals, with $a_N$ 1.49 mT and $a_H$ 0.988 mT, was assigned to the DMPO/phenoxyl radical adduct. The second 6-line signal with $a_N$ 1.55 mT and $a_H$ 2.22 mT was assigned to carbon-centered radical adduct. When 3,3,5,5-tetramethylpyrrolin-N-oxide (TMPO), was employed in the place of DMPO, another broad asymmetric 5-line signal was detected with $2A_{max}=3.99\;mT$ and $2A_{min}=3.04\;mT$, which is virtually identical to that obtained from the DMPO system The shape of the spectrum of the TMPO adduct changed drastically, with lapse of time resulting in a broad singlet after 40 min. The broad singlet was assigned to the porphyrin radical adduct. Incubation of globin with Fenton reagent in the presence of DMPO initially gave a DMPO/OH signal. Then, a new 12-line signal began to grow after one minute and fixed after 15 min. coexisting with the DMPO/OH signal, This 12-line signal was assigned to DMPO/phenoxyl with $a_N$ 1.47 mT, $a_{{\beta}H}$ 0.99 mT and $a_{{\gamma}H}$ 0.13 mT. A minor concentration of carbon-centered radical adduct was also detected. This radical composition is identical to that of guanidine HCl treated MetMb/DMPO/$H_2O_2$ system, indicating that the radical producing conditions are somehow common in both systems. Heme iron can be released by excess $H_2O_2$ in the MetMb/$H_2O_2$ system, providing for Fenton reagent. When MetMb was pretreated with tyrosine blocking agent, $KI_3$ the broad 5.line MetMb-derived signal was not detected in the MetMb/DMPO/$H_2O_2$ system, whereas no such effect was detected on such system of Hb in which the radical center was assigned to cysteine residue not tyrosine, indicating that tyrosine residue is a main radical center produced in the MetMb/$H_2O_2$ system Thus, the present data strongly support the previous indication that the apomyoglobin-derived radical center formed in the reaction of MetMb with $H_2O_2$ is a tyrosine residue.

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Evaluation the Output Dose of Linear Accelerator Photon Beams by Blind Test with Dose Characteristics of LiF:Mg,Cu,P TLD (LiF:Mg,Cu,P 열형광선량계의 선량특성을 이용한 눈가림법에 의한 출력선량 평가)

  • Choi, Tae-Jin;Lee, Ho-Joon;Yie, Ji-Won;Oh, Young-Gi;Kim, Jin-Hee;Kim, Ok-Bae
    • Progress in Medical Physics
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    • v.20 no.4
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    • pp.308-316
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    • 2009
  • To achieve the accurate evaluation of given absorbed dose from output dose of linear accelerator photon beam through investigate the characteristics of LiF:Mg,Cu,P TLD powder. This experimental TL phosphor is performed with a commercial LiF:Mg,Cu,P powder (Supplied by PTW) and TL reader (LTM, France). The TLD was exposed to 6 MV X rays of linear accelerator photon beam with range 15 to 800 cGy in blind dose at two hospitals. The dose evaluation of TLD was through the experimental algorithms which were dose dependency, dose rate dependency, fading and powder weight dependency. The glow curve has shown the three peaks which are 110, 183 and 232 degrees of heating temperature and the main dosimetric peak showed highest TL response at 232 high temperature. In this experiments, the LiF:Mg,Cu,P phosphor has shown the 2.5 eV of electron trap energy with a second order. This experiments guided the dose evaluation accuracy is within 1% +2.58% of discrepancy. The TLD powder of LiF:Mg,Cu,P was analyzed to dosimetric characterists of electron captured energy and order by glow shape, and dose-TL response curve guided the accuracy within 1.0+2.58% of output dose discrepancy.

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Open Heart Surgery of Congenital Heart Diseases -Report of Four Cases- (선천성심질환(先天性心疾患)의 심폐기(心肺器) 개심수술(開心手術) - 4례(例) 보고(報告) -)

  • Kim, Kun Ho;Park, Young Kwan;Jee, Heng Ok;Kim, Young Tae;Rhee, Chong Bae;Chung, Yun Chae;Oh, Chull Soo
    • Journal of Chest Surgery
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    • v.9 no.1
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    • pp.1-9
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    • 1976
  • The present. study reports four cases of congenital heart diseases, who received open heart surgery by the Sarn's Heart-Lung-Machine in the department of Thoracic Surgery, Hanyang University Hospital during the period between July 1975 and May 1976. The Heart-Lung-Machine consisted of the Sarn's five head roller pump motor system (model 5000), heat exchanger, bubble trap, the Rygg-Kyvsgaard oxygenator, and monitors. The priming of pump oxygenator was carried out by the hemodilution method using Hartman's solution and whole blood. Of the four cases of the heart diseases, three whose body weight were below 30kg, received the partial hemodilution priming and the remaining one whose body weight was 52kg received the total hemodilution priming with Hartman's solution alone. The rate of hemodilution was in the average of 60.5ml/kg. Extracorporeal circulation was performed at the perfusion flow rate of the average 94.0ml/kg/min, and at the moderate hypothermia between 35'5"C and 30'5"C of the rectal temperature. In the total cardiopulmonary bypass, arterial blood pressure was anged between 30 mmHg and 85 mmHg, generally maintaining over 60 mmHg and venous pressure was measured between 4 and $23cmH_2O$, generally maintaining below $10cmH_2O$. The first case: The patient, a nine year old girl having the symptoms and physical signs typical to cardiac anomaly was definitely diagnosed as isolated pulmonary stenosis through the cardiac catheterization. There was, however, no cyanosis, no pathological finding by X-ray and E.C.G. tracings. The valvulotomy was performed through the arteriotomy of pulmouary artery under the total cardiopulmonary bypass. Postoperative course of the patient was uneventful, and murmur and the clinical symptoms disappeared. The second case: A 12 year old boy with congenital heart anomaly was positively identified as having ventricular septal defect through the cardiac catheterization. As in the case with the first case, the patient exhibited the symptoms and physical signs typical to cardiac anomaly, but no pathological abnormality by X-ray and E.C.G. tracings. The septal defect was localized on atrioventricular canal and was 2 by 10 mm in size. The septal defect was closed by direct simple sutures under the cardiopulmonary bypass. Postoperative hemodynamic study revealed that the pressure of the right ventricle and pulmonary artery were decreased satisfactory. Postoperative course of the patient was uneventful, and murmur and the clinical symptoms disappeared. The third case: The patient, a 19 year old girl had been experienced the clinical symptoms typical to cardiac anomaly for 16 years. The pink tetralogy of Fallot was definitey diagnosed through the cardiac catheterization. The patient was placed on an ablolute bed rest prior to the operation because of severe exertional dyspnea, fatigability, and frequent syncopal attacks. However, she exhibited very slight cyanosis. Positive findings were noted on E.C.G. tracings and blood picture, but no evidence of pathological abnormality on X-ray was observed. All of the four surgical approaches such as Teflon patch closure (3 by 4cm in size) of ventricular septal defect, myocardial resection of right ventricular outflow tract, valvulotomy of pulmonary valvular stenosis, and pericardial patch closing of ventriculotomy wound were performed in 95 minutes under the cardiopulmonary bypass. Postoperative hemodynamic study revealed that the pressure of the right ventricle was decreased and pulmonary artery was increased satisfactorily. Postoperative course of the patient was uneventful, and murmur and the clinical symptoms disappeared. The fourth case: The patient, a 7 1/4 year old girl had the symptoms of cardiac anomaly for only three years prior to the operation. She was positively identified as having acyanotic tetralogy of Fallot by open heart surgery. The patient showed positive findings by X-ray and E.C.G. tracings, but exhibited no cyanosis and normal blood picture. All of the three surgical approaches, such a myocardial resection of hypertrophic sight ventricular outflow tract, direct suture closing of ventricular septal defect and pericardial patch closing of ventriculotomy wound were carried out in 110 minutes under the cardiopulmonary bypass. Postoperative hemodynamic study revealed that the pressure of the right ventricle was decreased and pulmonary artery was increased satisfactorily. Postoperative course of the patient was uneventful, and the symptoms disappeared.

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