• Asian Pacific Journal of Cancer Prevention
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• v.17 no.2
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• pp.781-784
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• 2016

The Plurinational State of Bolivia (Bolivia) has a high incidence rate of gallbladder cancer (GBC). However, the genetic and environmental risk factors for GBC development are not well understood. We aimed to assess whether or not cytochrome P450 (CYP1A1), glutathione S-transferase mu 1 (GSTM1), theta 1 (GSTT1) and tumor suppressor protein p53 (TP53) genetic polymorphisms modulate GBC susceptibility in Bolivians. This case-control study covered 32 patients with GBC and 86 healthy subjects. GBC was diagnosed on the basis of histological analysis of tissues at the Instituto de Gastroenterologia Boliviano-Japones (IGBJ); the healthy subjects were members of the staff at the IGBJ. Distributions of the CYP1A1 rs1048943 and TP53 rs1042522 polymorphisms were assayed using PCR-restriction fragment length polymorphism assay. GSTM1 and GSTT1 deletion polymorphisms were detected by a multiplex PCR assay. The frequency of the GSTM1 null genotype was significantly higher in GBC patients than in the healthy subjects (odds ratio [OR], 2.35; 95% confidence interval [CI], 1.03-5.37; age-adjusted OR, 3.53; 95% CI, 1.29-9.66; age- and sex-adjusted OR, 3.40; 95% CI, 1.24-9.34). No significant differences were observed in the frequencies of CYP1A1, GSTT1, or TP53 polymorphisms between the two groups. The GSTM1 null genotype was associated with increased GBC risk in Bolivians. Additional studies with larger control and case populations are warranted to confirm the association between the GSTM1 deletion polymorphism and GBC risk suggested in the present study.

• Journal of Korean Society of Environmental Engineers
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• v.30 no.6
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• pp.666-672
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• 2008

Feasibility of simultaneous removal of organic matter, nitrogen and phosphorus was evaluated by applying AO$_2$ system to treat wastewater from a seafood processing plant. Treatability test was conducted by incorporating activated sludge from municipal sewage treatment plant with PU-AC media. Inflow concentrations of COD, TN, and TP were 198$\sim$1,240 mg/L, 75$\sim$577.4 mg/L, and 2.2$\sim$53.5 mg/L, respectively. Average removal efficiencies and outflow concentration of COD, TN, and TP were 86.5%, 65.7 mg/L; 81.4%, 53.1 mg/L; and 80.6% 4.07 mg/L, respectively. Stable operation was possible by increasing organic matter, nitrogen, and phosphorus loading rate to seafood wastewater treatment system composed of anaerobic and aerobic reactors. Used PU-AC media was proved to be biodegradable in this AO$_2$ system by maintaining high biomass concentration in the PU-AC media.

• Korean Journal of Ecology and Environment
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• v.42 no.2
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• pp.153-160
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• 2009

A phytoplankton cultivation system was installed and operated for removal of nutrients from stream water polluted by nonpoint source pollution. The system was a continuous-flow culture comprising a phytoplankton tank that received inflow from a storage reservoir. When the system was operated as a batch culture for the purpose of determining hydraulic retention time (HRT), the proper HRT value was three days, and the removal rate of TP and TN averaged 70% and 44%, respectively. When the system was operated with the continuous flow from a stream for 45 days, 53.9% of TP and 53.1% of TN were removed as sludge. Due to active growth of phytoplankton, pH and dissolved oxygen in the phytoplankton tank were extremely high, reaching 10.8 and 16mg $L^{-1}$, respectively. It was concluded that nutrients can be effectively removed from polluted stream waters by cultivating phytoplankton.

• Journal of The Korean Society of Agricultural Engineers
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• v.53 no.6
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• pp.137-144
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• 2011

We have examined the pollutant load of combined sewer in dry days. Two monitoring sites (C-1, C-2) were equipped with an flowmeter. Monitoring was conducted from September 2004 to April 2006 for 20 months during dry days. Flowrate and concentration of pollutants are lowest from 3 to 6 o'clock, and it is high at 9, 12 and 21 o'clock. Most of organic matters exist in the dissolved state. The results show that pollution loads in C-2 area were 4.5-7.2 times higher than in C-1. Pollutant loads were high on Saturdays. Pollutant loads (kg/ha/day) of C-2 were 1.1-3.1 times higher those of C-1. However there was no significant difference in winter. Analysis of correlation coefficient of water quality parameter indicates that besides of flowrate all items in C-1 site have tight relationship respectively (p<0.01). In C-2 site, correlation coefficient of TP-SS, TP-TN, TP-flowrate, BOD-flowrate, BOD-SS and TN-TP are statistically significant (p<0.01).

• Asian Pacific Journal of Cancer Prevention
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• v.15 no.19
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• pp.8413-8422
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• 2014

Background: To investigate the relationship of five TP53 polymorphisms (p.P47S, p.R72P, PIN3 ins16bp, p.R213R and r.13494g>a) with the esophageal cancer (EC) risk in North Indians. Materials and Methods: Genotyping of p.P47S, p.R72P, PIN3 ins16bp, p.R213R and r.13494g>a polymorphisms of TP53 in 136 sporadic EC patients and 136 controls using polymerase chain reaction and PCR-RFLP. Results: The frequencies of genotype RR, RP and PP of p.R72P polymorphism were 16.91 vs 26.47%, 58.82 vs 49.27% and 24.27 vs 24.27% among patients and controls respectively. We observed significantly increased frequency of RP genotype in cases as compared to controls (OR=1.87, 95% CI, 1.01-3.46, p=0.05). The frequencies of genotype A1A1, A1A2 and A2A2 of PIN3 ins16bp polymorphism were 69.12 vs 70.59%, 27.20 vs 25% and 3.68 vs 4.41% among patients and controls. There was no significant difference among genotype and allele distribution between patients and controls. The frequencies of genotype GG, GA and AA of r.13494g>a polymorphism were 62.50 vs 64.70%, 34.56 vs 30.15% and 2.94 vs 5.15% among patients and controls respectively. No significant difference between genotype and allele frequency was observed in the patients and controls. For p.P47S and p.R213R polymorphisms, all the cases and controls had homozygous wild type genotype. The RP-A1A1-GG genotype combination shows significant risk for EC (OR=2.01, 95%CI: 1.01-3.99, p=0.05). Conclusions: Among the five TP53 polymorphisms investigated, only p.R72P polymorphism may contributes to EC susceptibility.

• Asian Pacific Journal of Cancer Prevention
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• v.15 no.16
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• pp.6871-6879
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• 2014

Background: The purpose of this study was to evaluate the potential association of five (p.P47S, p.R72P, PIN3 Ins16bp, p.R213R and r.13494g>a) polymorphisms of TP53 with the risk of developing breast cancer in North Indian Punjabi population. Methods: We screened DNA samples of 200 sporadic breast cancer patients (197 females and 3 males) and 200 unrelated healthy, gender and age matched individuals for the polymorphisms. Results: For the p.P47S polymorphism, we observed the PP genotype in 99.5% of the patients and PS genotype in only 1 patient. All the controls had the wild type PP genotype. The frequency of RR, RP and PP genotype of p.R72P was 23.5% vs 33.5%, 51.5% vs 45.5% and 25% vs 21% in patients and controls respectively. Heterozygous (RP) genotype was increased in breast cancer patients as compared to controls (51.5 vs 45.5%) and showed 1.61 fold significantly increased risk for breast cancer (OR=1.61, 95% CI, 1.01-2.58, p=0.04). In breast cancer patients the frequencies of A1A1, A1A2 and A2A2 genotypes of PIN3 Ins16bp polymorphism were 67%, 26% and 7% respectively whereas in controls the genotype frequencies were 68.5%, 27.5% and 4% respectively, with no significant difference. For p.R213R (c.639A>G), all individuals had homozygous wild type genotype. The frequencies of GG, GA and AA genotypes of TP53 r.13494g>a polymorphism were 62 vs 67.5%, 33 vs 28% and 5 vs 4.5% in patients and controls respectively, again without significant difference. We observed that RP-A1A1 genotype combination of p.R72P and PIN3 Ins16bp and RP-GG combination of p.R72P and r.13494g>a polymorphism showed significant risk of breast cancer (OR=1.65, 95%CI: 0.98-2.78, p=0.05; OR=1.72, 95%CI: 1.01-2.92, p=0.04). Conclusion: The results of present study indicated that among the five TP53 polymorphisms investigated, the p.R72P polymorphism, and the RP-A1A1 and RP-GG genotype combination contribute to breast cancer susceptibility in North Indians.

• Asian Pacific Journal of Cancer Prevention
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• v.16 no.18
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• pp.8397-8403
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• 2016

Background: Breast cancer (BCa) is the most common malignancy in Mexican women. A set of histopathological markers has been established to guide BCa diagnosis, prognosis and treatment. Nevertheless, in only a few Mexican health services, such as that of the Secretariat of National Defense (SEDENA for its acronym in Spanish), are these markers commonly employed for assessing BCa. The aim of this study was to explore the association of Ki67, TP53, HER2/neu, estrogenic receptors (ERs) and progesterone receptors (PRs) with BCa risk factors. Materials and Methods: Clinical histories provided background patient information. Immunohistochemical (IHC) analysis was conducted on 48 tissue samples from women diagnosed with BCa and treated with radical mastectomy. The Chi square test or Fisher exact test together with the Pearson and Spearman correlation were applied. Results: On average, patients were $58{\pm}10.4$ years old. It was most common to find invasive ductal carcinoma (95.8%), histological grade 3 (45.8%), with a poor Nottingham Prognostic Index (NPI; 80.4%). ERs and PRs were associated with smoking and alcohol consumption, metastasis at diagnosis and Ki67 expression (p<0.05). PR+ was also related to urea and ER+ (p<0.05). Ki67 was associated with TP53 and elevated triglycerides (p<0.05), and HER2/neu with ER+, the number of pregnancies and tumor size (p<0.05). TP53 was also associated with a poor NPI (p<0.05) and CD34 with smoking (p<0.05). The triple negative status (ER-/PR-/HER2/neu-) was related to smoking, alcohol consumption, exposure to biomass, number of pregnancies, metastasis and a poor NPI (p<0.05). Moreover, the luminal B subty was associated with histological type (p=0.007), tumor size (p=0.03) and high cholesterol (p=0.02). Conclusions: Ki67, TP53, HER2/neu, ER and PR proved to be related to several clinical and pathological factors. Hence, it is crucial to determine this IHC profile in women at risk for BCa. Certain associations require further study to understand physiological/biochemical/molecular processes.

• Journal of The Korean Society of Agricultural Engineers
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• v.53 no.6
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• pp.67-74
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• 2011

The objective of this study was to compare concentrations of nutrients such as total nitrogen (TN), nitrate nitrogen ($NO_3$-N) total phosphorous (TP), and phosphate phosphorous ($PO_4$-P) in groundwater under paddy and upland fields, and surface water recharging from a rural mixed land-use watershed. Chinese cabbage and hot pepper were cultivated on the upland field plot. The TN concentrations in upland groundwater showed double peaks (4.7, 4.3 mg/L, respectively) in April 2009 shortly after fertilizer application, indicating that TN concentrations are greatly influenced by fertilization. However, the TN concentrations in paddy groundwater were always lower than 2.0 mg/L irrespective of fertilization. Whereas the mean concentrations of TN and $NO_3$-N in upland groundwater significantly (p<0.05) higher than those in surface water, the mean concentrations of TP and $PO_4$-P in upland groundwater were significantly lower than those in surface water. On the other hand, the mean concentrations of TN, $NO_3$-N, TP and $PO_4$-P in paddy groudwater were significantly (p<0.05) lower than those in surface water. The TN concentrations in upland groundwater were generally higher than those in surface water during early April to early December due to the effect of fertilization, but vice versa in the other periods. In contrast, the TP concentrations in upland groundwater were always lower than those in surface water due to the sorption of inorganic phosphorous by soil. Moreover, the TN and TP concentrations in paddy groundwater were always lower than those in surface water, and therefore paddy groundwater may dilute nutrient concentrations in surface water when paddy groundwater and surface water mix.

• The Journal of Korean Academy of Prosthodontics
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• v.53 no.2
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• pp.138-143
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• 2015

Purpose: The aim of this study was to compare the translucency of two different laminate ceramic veneers with and without glazing. Materials and methods: Ten millimeter side square-shaped specimens in 0.3 mm and 0.6 mm thick were fabricated for the following materials with and without glazing (n=80): A1 shade IPS e.maxPress (IEM) and Styleveneers (STV). The color coordinates (CIE $L^*a^*b^*$) of the specimens were measured with a colorimeter. The Translucency parameter (TP) was calculated from the color difference of the material on a black versus a white background. For comparisons between materials and between the 'not glazed' and 'glazed' groups, unpaired t-test was used to analyze the data (P=.05). Results: The TP ($Mean{\pm}SD$) of 'not-glazed' and 'glazed' group of IEM specimens at 0.3 mm thickness were $45.99{\pm}3.00$ and $49.53{\pm}2.28$ and the TP at 0.6 mm thickness were $32.82{\pm}2.59$ and $43.02{\pm}0.98$, respectively. Likewise, the TP of 'not-glazed' and 'glazed' group of STV specimens at 0.3 mm thickness were $47.03{\pm}3.65$ and $50.95{\pm}3.05$ and the TP at 0.6 mm thickness group were $34.48{\pm}1.28$ and $43.39{\pm}1.20$, respectively. As the glazing of ceramic veneer differed, the TP of each ceramic veneer showed statistically significant difference. But, the result between the products was not statistically different. Conclusion: Within the limitations of this study, we are concluded that the glazing process changed translucency of laminate ceramic veneers and the TP would not be affected by products.

• BMB Reports
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• v.47 no.4
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• pp.221-226
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• 2014

Drug-resistance and imbalance of apoptotic regulation limit chemotherapy clinical application for the human hepatocellular carcinoma (HCC) treatment. The reactivation of p53 is an attractive therapeutic strategy in cancer with disrupted-p53 function. Nutlin-3, a MDM2 antagonist, has antitumor activity in various cancers. The post-translational modifications of p53 are a hot topic, but there are some controversy ideas about the function of phospho-$Ser^{392}$-p53 protein in cancer cell lines in response to Nutlin-3. Therefore, we investigated the relationship between Nutlin-3 and phospho-$Ser^{392}$-p53 protein expression levels in SMMC-7721 (wild-type TP53) and HuH-7 cells (mutant TP53). We demonstrated that Nutlin-3 induced apoptosis through down-regulation phospho-$Ser^{392}$-p53 in two HCC cells. The result suggests that inhibition of p53 phosphorylation on $Ser^{392}$ presents an alternative for HCC chemotherapy.