• Journal of Plant Biotechnology
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• v.37 no.2
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• pp.125-132
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• 2010

Rice is the staple food of more than 50% of the worlds population. Cultivated rice has the AA genome (diploid, 2n=24) and small genome size of only 430 megabase (haploid genome). As the sequencing of rice genome was completed by the International Rice Genome Sequencing Project (IRGSP), many researchers in the world have been working to explore the gene function on rice genome. Insertional mutagenesis has been a powerful strategy for assessing gene function. In maize, well characterized transposable elements have traditionally been used to clone genes for which only phenotypic information is available. In rice endogenous mobile elements such as MITE and Tos (Hirochika. 1997) have been used to generate gene-tagged populations. To date T-DNA and maize transposable element systems has been utilized as main insertional mutagens in rice. A main drawback of a T-DNA scheme is that Agrobacteria-mediated transformation in rice requires extensive facilities, time, and labor. In contrast, the Ac/Ds system offers the advantage of generating new mutants by secondary transposition from a single tagged gene. Revertants can be utilized to correlate phenotype with genotype. To enhance the efficiency of gene detection, advanced gene-tagging systems (i.e. activation, gene or enhancer trap) have been employed for functional genomic studies in rice. Internationally, there have been many projects to develop large scales of insertionally mutagenized populations and databases of insertion sites has been established. Ultimate goals of these projects are to supply genetic materials and informations essential for functional analysis of rice genes and for breeding using agronomically important genes. In this report, we summarize the current status of Ac/Ds-mediated gene tagging systems that has been launched by collaborative works from 2001 in Korea.

• Journal of Korea Port Economic Association
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• v.34 no.2
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• pp.69-82
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• 2018

The number of ships serviced at the container terminals in Busan is increasing by 2.9% per year. In spite of the increase in calling ships, there are no official records of waiting rate by the port authority. This study attempts to compare the theoretical ship waiting ratio and actual ship waiting ratio. The actual ship waiting ratio of container terminals is acquired from the 2014 to 2016 data of PORT-MIS and Terminal Operating System (TOS). Furthermore, methods and procedures to measure the actual ship's waiting rate of container terminal are proposed for ongoing measurement. In drawing the theoretical ship waiting ratio, the queuing theory is applied after deploying the ship arrival probability distribution and ship service probability distribution by the Chi Square method. As a result, the total number of ships waiting in a terminal for three years was 587, the average monthly service time and the average waiting time was 13.8 hours and 17.1 hours, respectively, and the monthly number of waiting ships was 16.3. Meanwhile, according to the queuing theory with multi servers, the ship waiting ratio is 31.1% on a 70% berth occupancy ratio. The reason behind the huge gap is the congested sailing in the peak days of the week, such as Sunday, Tuesday, and Wednesday. In addition, the number of waiting ships recorded on Sundays was twice as much as the average number of waiting ships.