• Journal of Microbiology and Biotechnology
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• 제19권3호
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• pp.217-228
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• 2009

Mobile genetic segments, or transposons, are also referred to as jumping genes as they can shift from one position in the genome to another, thus inducing a chromosomal mutation. According to the target site-specificity of the transposon during a transposition event, the result is either the insertion of a gene of interest at a specific chromosomal site, or the creation of knockout mutants. The former situation includes the integration of conjugative transposons via site-specific recombination, several transposons preferring a target site of a conserved AT-rich sequence, and Tn7 being site-specifically inserted at attTn7, the downstream of the essential glmS gene. The latter situation is exploited for random mutagenesis in many prokaryotes, including IS (insertion sequence) elements, mariner, Mu, Tn3 derivatives (Tn4430 and Tn917), Tn5, modified Tn7, Tn10, Tn552, and Ty1, enabling a variety of genetic manipulations. Randomly inserted transposons have been previously employed for a variety of applications such as genetic footprinting, gene transcriptional and translational fusion, signature-tagged mutagenesis (STM), DNA or cDNA sequencing, transposon site hybridization (TraSH), and scanning linker mutagenesis (SLM). Therefore, transposon-mediated genetic engineering is a valuable discipline for the study of bacterial physiology and pathogenesis in living hosts.

• 한국안전학회지
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• 제24권6호
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• pp.32-38
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• 2009

According to the statistics from the Ministry of labor, 70~80 people died by work-related electric shock accidents in the workplace and this number is about 10~20 times higher than those of the foreign countries such as Japan and the United Kingdom in frequencies and rates per 100,000. The electrocution deaths had decreased from several hundred in 1990s to 67 in 2002, since then there has been little change over the last 5 years. To reduce this number of the electrocution death, it is necessary to analyze the sources of the accidents and to implement the suitable measures for preventing the similar accidents from occurring. But there is not any consensus standard method or procedures for accident investigation yet. And the results solely depend on the ability of the investigator. Because of the demand of eliminating technical barrier for free trading, the IEC 60364 is adopted as a regulation for low voltage electrical installations in Korea. The facilities designed and constructed by IEC 60364 are increasing. The facilities by IEC 60364 have different features in comparison with the facilities by Korea eletro technical regulation, and these features combined with parameters of electric shock accident can greatly affect the consequences of the electric accidents. The accident analysis program for electric shock injuries in Terra Neutral(TN) and Terra Terra(TT) system was developed in this paper, which was based on KOSHA Electric Shock Analysis Program(ESAP).

• 조명전기설비학회논문지
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• 제23권5호
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• pp.36-41
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• 2009

본 논문에서는 건축물 전기설비의 IEC 60364를 기반으로 개발된 점검기법을 이용하여 실태 조사를 실시하였다. 점검기법 개발을 위한 분석대상은 기술기준에 준하여 시공된 국내 건축전기설비와 IEC 60364로 시공된 건축전기설비를 대상으로 차이점을 분석하였다. 측정 요소는 Loop impedance, 차단기 동작특성, 보호도체의 연속성을 측정하였다. 조사결과 국내 건축전기설비의 경우 TT시스템과 유사하지만 보호도체의 연속성에 문제가 있었다. TN-C-S로 시공된 건축전기설비의 경우 시공은 정확하게 되어 있었으나 담당자의 IEC 60364와 관련된 이해가 부족한 상태였다.

• 제어로봇시스템학회:학술대회논문집
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• 제어로봇시스템학회 1992년도 한국자동제어학술회의논문집(국제학술편); KOEX, Seoul; 19-21 Oct. 1992
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• pp.599-606
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• 1992

A manipulator system that needs significantly large workspace volume and high payload capacity has greater link flexibility than typical industrial robots and teleoperators. If link flexibility is significant, position control of the manipulator's end-effector exhibits the nonminimum phase, noncollocated, and flexible structure system control problems. This paper addresses inverse dynamic trajectory planning issues of a flexible manipulator. The inverse dynamic equation of a flexible manipulator was solved in the time domain. By dividing the inverse system equation into the causal part and the anticausal part, the inverse dynamic method calculates the feedforward torque and the trajectories of all state variables that do not excite structural vibrations for a given end-point trajectory. Through simulation and experiment with a single-Unk flexible manipulator, the effectiveness of the inverse dynamic method has been demonstrated.

• Asian-Australasian Journal of Animal Sciences
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• 제6권2호
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• pp.205-209
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• 1993

A new treatment system for animal waste water has been developed as an alternative to the activated sludge process. It consists of two treatments; one is operated with 7 tanks, and the other is soil and plant cultivation bed. Aerobic microorganisms are added to the influent water in the tanks where the water is aerated so that the microbes utilize the pollutants, while sedimentation removes the indigestible solids. In the secondary treatment the water, which has already received a primary treatment, is filtered through soil where it also receives treatment by soil organisms. In addition there is transpiration of water and absorption of minerals by plants. In the primary treatment BOD, SS, coliforms (E. coli), TP and total bacteria were removed 79-99%, but COD and TN were removed only 58% and 36%, respectively. In the secondary treatment removal of nutrients proceeded further, and 93-99% of pollutants were removed. The treated waters met the quality standard of discharge water in Japan except for TN, which was in too great a concentration to meet discharge standards. This problem requires further study.

• 한국수자원학회:학술대회논문집
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• 한국수자원학회 2007년도 학술발표회 논문집
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• pp.8-14
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• 2007

Water quality in Lake Rotorua, New Zealand, deteriorated since the 1960s because of excessive phytoplankton growths due principally to increasing nitrogen and phosphorus in the lake waters. Nutrient concentrations in eight of the nine major streams feeding Lake Rotorua have increased since 1965. The groundwater system has a key role in the hydrology of the Lake Rotorua catchment and the groundwater system is probably the control on the time delay between intensification of agricultural land use and response of surface water quality. All major, and many minor streams, in the catchment are fed by springs. Two lithological units are most important to groundwater flow in the Lake Rotorua catchment: Mamaku Ignimbrite, erupted in about 200,000 years ago and Huka Formation sediments which filled the caldera left by the Mamaku Ignimbrite eruption. Rainfall recharge to groundwater in the groundwater catchment of Lake Rotorua is estimated as approximately 17300 L/s. A calibrated steady-state groundwater flow model estimates that approximately 11100 L/s of this flow discharges into streams and then into the lake and the balance travels directly to Lake Rotorua as groundwater discharge through the lake bed. Land use has impacted on groundwater quality. Median Total Nitrogen (TN) values for shallow groundwater sites are highest for the dairy land use (5.965 mg/L). Median TN values are also relatively high for shallow sites with urban-road and cropping land uses (4.710 and 3.620 mg/L, respectively). Median TN values for all other uses are in the 1.4 to 1.5 mg/L range. Policy development for Lake Rotorua includes defining regional policies on water and land management and setting an action plan for Lake Rotorua restoration. Aims in the action plan include: definition of the current nutrient budget for Lake Rotorua, identification of nutrient reduction targets and identification of actions to achieve targets. Current actions to restore Lake Rotorua water quality include: treatment of Tikitere geothermal nitrogen inputs to Lake Rotorua, upgrade of Rotorua City sewage plant, new sewage reticulation and alum dosing in selected streams to remove phosphorus.

• Environmental Engineering Research
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• 제20권3호
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• pp.277-284
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• 2015

Experiments for highly concentrated contaminants in pig waste slurry were carried out for the feasibility test of a pilot-scale innovative process scheme of engaging autothermal thermophilic aerobic digestion (ATAD) and expended granular sludge bed (EGSB) followed by sequencing batch reactor (SBR) system. Contaminants in pig waste slurry such as organic substance, total nitrogen (TN), ammonia nitrogen and total phosphorus (TP) contents were successfully reduced in the system. Total volatile solids (TVS) and chemical oxygen demands (COD) for organic matter in the feed were 32.92 g/L and 42.55 g/L respectively, and they were reduced by about 98.7% and 99.2%, respectively in the system. The overall removal efficiencies for TN and ammonium nitrogen were found to be 98.1 and 98.5%, respectively. The overall removal efficiency for total phosphorus was also found to be 92.5%. Faecal coliform density was reduced to <$1.2{\times}10^4CFU/g$ total solids. Biogas and $CH_4$ were produced in the range of 0.39-0.85 and $0.25-0.62m^3/kg$ [VS removed], respectively. The biogas produced in the system comprised of $295{\pm}26ppm$ (v/v) [$H_2S$].

• 한국수자원학회논문집
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• 제48권2호
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• pp.79-90
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• 2015

2013년 3월에 발사된 Landsat 8 인공위성의 이미지데이터를 이용하여 금강유역을 대상으로 수질인자에 대한 평가를 수행하였다. 본 연구의 목적은 다양한 수질인자 중 녹조에 직접적인 영향을 미치는 총질소와 총인의 농도를 추정함으로써 궁극적으로 수생태계에 악영향을 미치는 녹조의 발생을 모니터링 하는 것이다. 현장실측데이터와 인공위성 데이터간의 상관관계를 규명하기 위하여 Pearson' 상관계수를 이용하여 그 관계를 파악하였다. Landsat 8이 촬영되는 시기를 포함하는 총 20개의 현장실측 데이터가 수집되었으며 Landsat 8의 11개의 밴드중, 밴드2, 3, 4의 반사도 값이 총인과 총질소를 탐지하는데 있어서 가장 상관성 높은 것으로 나타났다. 총질소는 유의수준 0.05에서 밴드2(0.48), 3(0.62), 4(0.57)과 높은 양의 상관관계를 보였으며, 총인의 경우, 유의수준 0.01에서 밴드2(0.59), 3(0.59), 4(0.58)로 높은 양의 상관관계를 나타냈다. 5번 밴드는 유의수준을 벗어남으로써 두 수질인자를 탐지하는데 상관성이 떨어지는 것으로 나타났다. 상관성이 높았던 밴드간의 조합을 통해서 총질소와 총인에 대한 각각의 최적 회귀식이 다중 회귀식을 근거로 구축되었다. 유도된 회귀식으로 계산된 총질소와 총인의 농도값은 통계기법인 Bias와RMSE를 이용하여 현장실측데이터들과 비교 검증되었다. 최종적으로, 2014년 4월 21과 2013년 11월 12일에 대한 맵핑을 수행함으로써 총질소와 총인의 공간적인 분포를 시각적으로 확인할 수 있었다.

• 한국습지학회지
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• 제18권3호
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• pp.292-300
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• 2016

담수호 수질오염방지를 위해 유역종합대책과 호내 대책으로 수질오염방지 종합대책 수립 및 인공습지를 적용하였다. 본 연구는 화성호 수질 개선 및 자연생태계 보호를 위한 호소 수변지역 인공습지 조성에 따른 수질정화 효율과 인공습지 효율 제고를 위한 설계 고려사항을 도출하기 위하여 수행하였다. 연구대상 시설은 경기도 화성시에 위치한 인공습지 시스템으로forebay와 wetland로 구성되어 있다. 모니터링은 강우시 2회, 건기시 10회 수행하였으며, 분석 결과 인공습지 유입 및 유출의 유속은 COD와 TN의 부하량과 높은 상관성을 가지는 것으로 분석되었다. 인공습지의 forebay 지점에서 건기시에는 COD, TN 및 TP의 농도가 감소하는 것으로 조사되었으며, 비 강우시에 비해 강우시에 저감효율이 상대적으로 높은 것으로 나타났다. 또한, 강우시에 인공습지에 유입되는 양에 비해 유출되는 양이 상당히 작은 것으로 강우유출수 저감 효과에 뛰어난 것으로 분석되었다. 건기시 및 강우시의 인공습지의 전반적인 효율의 경우 TSS는 50% 이상, TP는 20~35%으로 조사되었으며, TN은 26~94%로써 다른 오염물질에 비하여 높은 저감효율을 나타냈다. 이러한 모니터링 자료는 향후 담수호 수질정화효율 증대를 위한 인공습지 설계 시 중요한 자료를 제공할 것으로 판단된다.

• 한국물환경학회지
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• 제23권5호
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• pp.740-747
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• 2007

The advanced wastewater treatment plant of leather industry was selected to evaluated with its effluent water quality and statistical characteristics. Most of pollutants removal efficiencies were over 90% as well. And 95% reliability of effluent concentration were 106.8 mg/L of $COD_{mn}$, 72.04 mg/L of TN. However Effluent quality of TN exceeds the regulated limit. The range of coefficient of variation (CV) were between 0.18 and 2.49. Also, coefficient of reliability (COR) were between $0.03(BOD_5){\sim}0.63(COD_{mn})$ and 0.43 in terms of T-N, $Z_{l-a}$(Normalized Percentiles) value were 55.7 and 2.25 in terms of $BOD_5$ and T-N as shown in the following table.