• Journal of Microbiology
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• v.42 no.2
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• pp.139-142
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• 2004

To identify genes involved in the decolorization of brilliant green, we isolated random mutants generated by transposon insertion in brilliant green-decolorizing bacterium, Citrobacter sp. The resulting mutant bank yielded 19 mutants with a complete defect in terms of the brilliant green color removing ability. Southern hybridization with a Tn5 fragment as a probe showed a single hybridized band in 7 mutants and these mutants appeared to have insertions at different sites of the chromosome. Tn5-inserted genes were isolated and the DNA sequence flanking Tn5 was determined. By comparing these with a sequence database, putative protein products encoded by bg genes were identified as follows: bg 3 as a LysR-type regulatory protein; bg 11 as a MalG protein in the maltose transport system; bg 14 as an oxidoreductase; and bg 17 as an ABC transporter. The sequences deduced from the three bg genes, bg 2, bg 7 and bg 16, showed no significant similarity to any protein with a known function, suggesting that these three bg genes may encode unidentified proteins responsible for the decolorization of brilliant green.

• Proceedings of the Korean Institute of Electrical and Electronic Material Engineers Conference
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• 2007.11a
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• pp.400-400
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• 2007

In this study, we investigated liquid crystal (LC) alignment with ion beam (IB) that non contact alignment technique on polyimide and electro-optical characteristics of twisted nematic (TN)-liquid crystal display (LCD) on the polyimide under various ion beam angles. In this experiment, polyimide layer was coated on glass by spin-coating and Voltage-transmittance(VT) and response time characteristics of the TN cell were measured by a LCD evaluation system. The good characteristics of the nematic liquid crystal (NLC) alignment with the ion beam exposured polyimide surface was observed. In addition, it can be achieved the good EO properties, and residual DC property of the ion beam aligned TN cell on polyimide surface.

• Journal of Korean Society of Environmental Engineers
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• v.35 no.5
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• pp.381-387
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• 2013

Nitrogen and phosphorus removal was investigated in an UASB-ABF (Up-flow anaerobic sludge blanket - aerated bio-filter) anaerobic sewage treatment system. Successful removal of nitrogen and phosphorus with organic matters was possible in the UASB-ABF system from the results of 160 days operation with the influent raw domestic sewage. Removal efficiencies of organic matter (as TCOD) showed 64% in UASB without recycle of the ABF effluent, however, they increased to 92%, 95%, 96% with 120%, 180% and 240% recycle of the ABF effluent, respectively. Increasement of the organic matter removal was not prominent at recycle ratio above 180%. Apparent increase in TN removal occurred with recycle of the ABF effluent. TN removal efficiency was 18% without recycle, but it increased to 82% with 240% recycle of the ABF effluent. And stable nitrification above 95% was possible as a result of efficient removal of organic matter in the UASB with and without recycle of the effluent. Removal of both TP and $PO{_4}^{3-}$-P also increased remarkably with recycle of the effluent. Without recycle of the effluent, that is at strict anaerobic condition in UASB, TP was not removed, however, its removal efficiency increased to 51%, 63%, 71% at recycle ratios of 120%, 180%, 240%, respectively mainly at UASB.

• Korean Journal of Ecology and Environment
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• v.56 no.4
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• pp.339-347
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• 2023

Dissolved organic matter (DOM) is a key component in the biogeochemical cycling in freshwater ecosystem. However, it has been rarely explored, particularly complex river watershed dominated by natural and anthropogenic sources, such as various effluent facility and livestock. The current research developed a new analytical method for TOC/TN (Total Organic Carbon/Total Nitrogen) stable isotope ratio, and distinguish DOM source using stable isotope value (δ¹³C-DOC) and spectroscopic indices (fluorescence index [FI] and biological index [BIX]). The TOC/TN-IR/MS analytical system was optimized and precision and accuracy were secured using two international standards (IAEA-600 Caffein, IAEA-CH-6 Sucrose). As a result of controlling the instrumental conditions to enable TOC stable isotope analysis even in low-concentration environmental samples (<1 mgC L^-1), the minimum detection limit was improved. The 12 potential DOM source were collected from watershed, which includes top-soils, groundwater, plant group (fallen leaves, riparian plants, suspended algae) and effluent group (pig and cow livestock, agricultural land, urban, industry facility, swine facility and wastewater treatment facilities). As a result of comparing characteristics between 12 sources using spectroscopic indices and δ¹³C-DOC values, it were divided into four groups according to their characteristics as a respective DOM sources. The current study established the TOC/TN stable isotope analyses system for the first time in Korea, and found that spectroscopic indices and δ¹³C-DOC are very useful tool to trace the origin of organic matter in the aquatic environments through library database.

• Journal of Korea Water Resources Association
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• v.46 no.11
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• pp.1115-1128
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• 2013

The purpose of this study is to evaluate the suspended solids and eutrophication processes relationships in Chungju lake using CE-QUAL-W2, two-dimensional (2D) longitudinal/vertical hydrodynamic and water quality model. For water quality modeling, the lake segmentation was configured as 7 branches system according to their shape and tributary distribution. The model was calibrated (2010) and validated (2008) using 2 years of field data of water temperature, suspended solids (SS), total nitrogen (TN), total phosphorus (TP) and algae (Chl-a). The water temperature began to increase in depth from April and the stratification occurred at about 10 m early July heavy rain. The high SS concentration of the interflow density currents entering from the watershed was well simulated especially for July 2008 heavy rainfall event. The simulated concentration range of TN and TP was acceptable, but the errors might occur form the poor reflection for sedimentation velocity of nitrogen component and adsorption-sediment of phosphorus in model. The concentration of Chl-a was simulated well with the algal growth patterns in summer of 2010 and 2008, but the error of under estimation may come from the use of width-averaged velocity and concentration, not considering the actual to one side inclination by wind effect.

• Korean Journal of Environmental Biology
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• v.26 no.3
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• pp.252-263
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• 2008

The purpose of this study was to determine trophic state, based on nutrients (TN, TP), transparency (SD), and chlorophyll-${\alpha}$ (Chl) and identify their empirical relations of TN-Chl, TP-Chl and Chl-SD depending on the dataset used along with dynamics of conductivity and suspended solids. Analysis of trophic states showed that more than half of 36 reservoirs were judged as eutrophic-hypertrophic conditions depending on the trophic variables. Seasonal values of TP varied by nearly 500% and showed greater in August than any other months. In contrast, TN varied within less than 90% and all monthly mean values of TN were never fall less than 1.2 mg L$^{-1}$ indicating low seasonal variations and high ambient concentrations (eutrophic-hypertrophic state). Analysis of empirical relations in the trophic variables showed that transparency had greater functional relations with Chl (R$^2$=0.31, p<0.001) than TP (R$^2$=0.15, p<0.001) and TN (R$^2$=0.20, p<0.001). Ratios of TN : TP in the ambient water indicated that most reservoirs showed a potential phosphorous limitation on the algal growth. Thus, algal biomass, based on Chl values, was more regulated by phosphorous than nitrogen. Analysis of linear regression model, based on log-transformed annual mean values, showed that only 30% in the variation of Chl was explained by TP (R$^2$=0.295, p=0.001, n=36) and 15% by TN (R$^2$=0.151, p=0.019, n=36). However, linear regression model, based on individual system, showed that Chl-TP model had strong positive relations (R$^2$=0.62, p=0.002, n=12), whereas the model had no any relations (p=0.892, n=12). Overall, our data suggested that averaging effect in the empirical model developments may influence the significance in the statistical analysis.

• Journal of Environmental Science International
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• v.25 no.9
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• pp.1233-1239
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• 2016

This study aimed to evaluate changes in the TN and TP removal efficiencies, depending on whether or not a settling process is applied, in a sequencing batch reactor (SBR) process with a membrane bioreactor (MBR). Nutrient removal was considered in terms of developing an advanced water treatment system for ships in accordance with water quality standards set forth by 227(64). For these purposes, the TN and TP concentrations in the inflow and outflow water were measured to calculate the TN and TP removal efficiencies, depending on whether or not a settling process was used. Water discharged from a bathroom, which was constructed for the experiment, was used as the raw water. The experiment that included a settling process was conducted twice, and the operating conditions were: aeration for 90 min, settling for 30 min, agitation for 15 min, and settling for 15 min for one experiment; and aeration for 150 min, settling for 45 min, agitation for 15 min, and settling for 15 min in the other. Operating conditions for the experiment that did not include a settling process were: aeration for 180 min and agitation for 60 min. The concentration of the mixed liquor suspended solids (MLSS) in the reactor was 3,500 mg/L, while the aeration rate was 121 L/min and the water production rate was 1.5 L/min. For the two experiments where a settling process was applied, the average TN removal efficiencies were 44.39% and 41.05%, and the average TP removal efficiencies were 47.85% and 46.04%. For the experiment in which a settling process was not applied, the average TN removal efficiency was 65.51%, and the average TP removal efficiency was 52.51%. Although the final nutrient levels did not satisfy the water quality standards of MEPC 227(64), the TN and TP removal efficiencies were higher when a settling process was not applied.

• Journal of Korea Water Resources Association
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• v.48 no.2
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• pp.79-90
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• 2015

In this study, the water quality parameters of Geum-river were estimated using Landsat 8 satellite image data which had launched in March 2013. The goal of this research is to predict HAB and to monitor spatial pattern of total nitrogen (TN) and total phosphorus (TP) because both TN and TP are the dominant factors of the growth of harmful algal blooms (HABs). To investigate the relationship between satellite band reflectance and in situ measurement value, Pearson' correlation coefficient analysis was used. The band2, 3, 4 and 5 reflectance values among 11 bands of Landsat 8 were used which was highly associated with detecting TN and TP. The 20 in situ data set with satellite's overpass time were identified. TN showed positive relation with band 2 (0.48), band3 (0.62), band4 (0.57) at a significance level of p<0.05. TP also showed high correlation for band2 (0.59), band3 (0.59), band4 (0.58) at a significance level of p<0.01. The optimal regression equation models were constructed for TN and TP based on multiple regression equations. The estimated concentration based on derived regression equations of TN and TP were compared with in situ measurement data. Finally, the spatial pattern of the two parameters was able be monitored through mapping on November 12, 2013 and April 21, 2014.

• Journal of Korean Society on Water Environment
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• v.20 no.6
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• pp.657-663
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• 2004

The aims of this study are the characterization of runoff from nonpoint source, the analysis of the pollutant loads and an establishment of a management plan for nonpoint source of Okcheon. For this purpose the basin of the stream So-okcheon was selected to the investigated. During the period from May 29 to July 21 in 2003, the water automatic sampler system has been installed in Okkagkyo and parameters such as SS, COD, TOC, TP and TN were analyzed. The pollutants of nonpoint source seem to be washed out along the stream water in the beginning of rainfall, remain in water and cause the stream pollution. The runoffs during heavy rainfall, especially, much higher concentration of SS than those during dry period. With respect to the annual loading of pollutants of the nonpoint source, the COD was 124 ton/yr, TOC 396 ton/yr, TN 1,429 ton/yr and TP 4.2 ton/yr in the year 2002. With respect to the pollutants loading of the nonpoint source, the COD was 375 ton/yr(95% of the total COD loading of 394 ton/yr), TOC 844 ton/yr(96% of the tatal TOC loading of 876 ton/yr), TN 1,985 ton/yr(96% of the total TN loading of 2,062 ton/yr) and TP 37.1 ton/yr(92% of the total TP loading of 40.3 ton/yr) in the year 2003.

• Journal of Environmental Science International
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• v.24 no.8
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• pp.1045-1053
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• 2015

This study evaluated the effect of water level of water resources on water quality in Ulsan. Two reservoirs, Sayeon Dam and Hoeya Dam, were selected and water quality of chemical oxygen demand (COD), total nitrogen (TN) and total phosphorus (TP) were analyzed from 2012 to 2014. And the characteristics of precipitation were also analyzed for 70 years (1945~2014) because runoff of non-point pollutant was strongly affected by precipitation. As a result, water deterioration of Sayeon Dam and Hoeya Dam were affected in accordance with lowering water level. For example, the concentrations of COD and TN was negatively correlated with the water level when the water level of Sayeon Dam was gradually decreased in 2013. The TN concentration was increased to 1.432 mg/L from 0.875 mg/L while the lowest water level of Sayeon Dam was recorded 45 m in 2014. Additionally the concentration of COD and TN was sensitively increased with 0.213 mg/L/m and 0.058 mg/L/m on account of non-point pollutant runoff. It is indicated that hereafter a control of non-point pollutant runoff is the critical factors to maintain water resources because the contribution of non-point pollutant is expected to increase due to the frequent heavy rain events. Therefore, it is necessary to map out a specific plan for non-point pollutant control based on analyses of runoff characteristics, water pollution sources and reduction plans in water pollutants and to establish a water modelling and database system as a preventive action plan.