• 산업기술연구
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• 제38권1호
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• pp.15-19
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• 2018

In this study, hyaluronic acid (HA) was electro-spun to fabricate nanofibrous membranes for support materials. Because HA is very hygroscopic, it is difficult to obtain enough viscosity to apply electrospinning method. Therefore, we mixed HA with polyethylene oxide (PEO) to obtain proper viscosity and added adipic acid (ADH) to promote cross-linking of PEO/HA during electrospinning. The morphology of PEO/HA membrane was optimized according to PEO/HA concentration ratio and spinning rate. Finally, we fabricated gold-nanoparticle-embedded PEO/HA membranes and their catalyst activities were evaluated in tetramethylbenzidine (TMB) oxidation.

• 대한화학회지
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• 제67권6호
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• pp.415-419
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• 2023

In this study, we report the one-pot synthesis of reduced graphene oxide (rGO) containing platinum nanoparticles with catalytic activity to break down hydrogen peroxide as a peroxidase-mimicking catalyst. A single reducing agent was used to reduce graphene oxide and a platinum precursor at a moderately low temperature of 70℃. The rGO was homogeneously decorated with platinum nanoparticles. The catalytic activity of Pt-rGO was investigated for the oxidation of 3,3',5,5'- tetramethylbenzidine (TMB), a peroxidase substrate, in the presence of hydrogen peroxide. The Pt-rGO coupled with glucose oxidase was also able to detect glucose at millimolar concentrations (up to 1 mM). Our results show that the Pt-rGO composite is a promising catalyst for the detection of hydrogen peroxide. This method was also applied for the detection of glucose.

• 센서학회지
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• 제23권4호
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• pp.278-283
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• 2014

In this study, we propose an immunosensor using zinc oxide nanorods (NRs) inside PDMS channel for detecting the influenza A virus subtype H7N9. ZnO with high isoelectric point (IEP, ~9.5) makes it suitable for immobilizing proteins with low IEP. In this proposed H7N9 immunosensor structure ZnO NRs were grown on the PDMS channel inner surface to immobilize H7N9 capture antibody. A sandwich enzyme-linked immunosorbent assay (ELISA) method with was used 3,3',5,5' tetramethylbenzidine (TMB) for detecting H7N9 influenza virus. The immunosensor was evaluated by amperometry at various H7N9 influenza antigen concentrations (1 pg/ml - 1 ng/ml). The redox peak voltage and current were measured by amperometry with ZnO NWs and without ZnO NWs inside PDMS channel. The measurement results of the H7N9 immunosensor showed that oxidation peak current of TMB at 0.25 V logarithmically increased from 2.3 to 3.8 uA as the H7N9 influenza antigen concentration changed from 1 pg/ml to 1 ng/ml. And then we demonstrated that ZnO NRs inside PDMS channel can improve the sensitivity of immunosensor to compare non-ZnO NRs inside PDMS channel.