• Journal of the Korean Society of Tobacco Science
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• v.2 no.2
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• pp.1-7
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• 1980

Less-volatile organic components in leaves from aromatic tobaccos of different varieties, both Oriental and Korean types were isolated and concentrated using a simple apparatus with fewer manipulations. Each less-volatile concentrate was then subjected to spectrophotometric recording in the visible range, to thin-layer chromatographic group separation, and high-performance liquid chromatographic profile analysis. The methods allow detection of significant quantitative differences in visible absorption spectra, TLC patterns, and high resolution HPLC profiles among varieties.

• Journal of Food Hygiene and Safety
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• v.3 no.3
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• pp.105-109
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• 1988

To develop a method for detecting and e&timating the quantity of adulterant rapeseed oil in sesame oil, five kinds of sesame oils and three kinds of rapeseed oils collected from different sources were fractionated by TLC (thin layer chromatography) and separated on the basis of PN (partition number) by HPLC (high performance liquid chromatography). These obsenations indicate that the proportion of adulterant rapeseed oil when mixed minimum 4% with sesame oil can be detected.

• Journal of the Korean Society of Food Science and Nutrition
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• v.32 no.7
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• pp.1164-1167
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• 2003

Aristolochic acid has been known to be a carcinogenic compound and to cause Chinese herbs nephrophathy(CHN). To determine the content of aristolochic acid in various medicinal herbs marketed domestically, samples of Aristolochia fangchi, Aristolochia fructus, Aristolochia radix, Asiasari radix, Saussureae radix, and Akebia caulis were analyzed using TLC and HPLC. The optimal conditions for TLC and HPLC using $C_{18}$ column were established for the quantitative analysis of aristolochic acid. This study showed that Aristolochia fangchi and Aristolochia fructus examined in this study contain aristolochic acid of 3.9 and 2.3 mg/g sample, respectively. In contrast, aristolochic acid was not detected in the other samples.

• Natural Product Sciences
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• v.11 no.2
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• pp.109-114
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• 2005

Leucas aspera Link. (Lamiaceae) is an important medicinal plant in indigenous systems of medicine in India and commonly known as 'Thumbai'. It has various ethnomedicinal values as various traditional communities find diverse medicinal properties. It is used as antipyretic, stimulant, expectorant and diaphoretic drug. The present communication deals with the detailed pharmacognostical evaluation of whole plant of L. aspera collected from five different geographical zones of the country-Uttar Pradesh, Orissa, Karnataka, West Bengal and Gujarat. The botanical characters and TLC fingerprint profile of all the samples were quite similar but some variations were observed in physicochemical parameters. However, some microscopical characters and TLC profile can be used as diagnostic characters for identification of L. aspera, for example amphistomachic leaves and two types of trichomes-abundant, non glandular, uniseriate, 1-3 celled and few glandular, 2-5 celled stalk with rounded tip. Presence of some components at $R_fs-0.56$, 0.65 and 0.76 under UV 366 and at $R_fs-0.31$, 0.43, 0.60, 0.76 and 0.82 under visible light after derivetization in TLC profile may also be used as diagnostic character.

• Korean Journal of Pharmacognosy
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• v.20 no.2
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• pp.88-95
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• 1989

Traditional Chinese herbal drugs, namely, Atractylodes Rhizome and Atractylodes Rhizome White were identified by means of chemical analysis such as TLC and GC/MS. In Korea, the old-grown and the newly-grown rhizomes of Atractylodes japonica Koidzumi (Compositae) are used as the Atractylodes Rhizome and the Atractylodes Rhizome White, respectively. Both rhizomes contain the atractylon asa major spot on TLC. The content of atractylon in a newly-grown rhizome of A. japonica (Atractylodes Rhizome White called in Korea) appears to contain much larger quantity in comparison with that of an old-growon one (Atractylodes Rhizome). The TLC spot pattern analysis showed that rhizomes of A. japonica and A. macrocephala contain the atractylon as a major indicator spot, but no atractylodin (extreamly small amount). However, rhizomes of A. lancea De Candolle and A. koreana Kitamura contain the atractylodin as a major indicator spot.

• Analytical Science and Technology
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• v.12 no.3
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• pp.243-247
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• 1999

After aconitine-type alkaloids have been isolated from the crude drug "Buza" as the precipitate, they could be identified with colour test, UV/Vis. absorption wavelength, TLC. The presence of aconitine-type alkaloids are confirmed by the reaction with the Reineke ion, $[Cr(NH_3)_2(SCN)_4]^{1+}$, and TCR ion, $[Co(SCN)4]^{2-}$, to produce the white precipitate or one of the containing from blue to yellowish blue. It is based on the formation of complex compounds by aconitine-type alkaloids with Reineke and TCR reagent. The method has been found to be simple, convenient and suitable for routine identification of aconitine-type alkaloids, related basic compounds, the crude drug "Buza" processed from the roots of certain Aconitum spp. plants(Ranunculaceae).

• KSBB Journal
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• v.15 no.6
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• pp.673-676
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• 2000

In order to identification of carotenoid pigments of Haloarcular sp. EH-1 as a food for fish were analyzed. The content of carotenoids cultured in 3 L and 5 L bioreactor were 83.1 and 82.7 mg%, respectively. Identification of each carotenoid was achieved by means of co-TLC and co-HPLC with authentic specimens, spectroscopic and instrumental analyses, and chemical treatments as usual. The main components identified were ${\beta}$-carotene(8.1%), 3-hydroxyechinenone(42.0%) and astaxanthin(25.0%).

• Korean Journal of Pharmacognosy
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• v.20 no.3
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• pp.175-179
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• 1989

From crude drug preparation(Soshiho-Tang) ginseng sapogenins were identified by TLC and $ginsenoside-Rb_1$ was determined quantitatively by HPLC. Panaxadiol, pandaxatriol, acid-hydrolysates of ginseng saponin, were identified by TLC with benzene/acetone(4 : 1, v/v). Rf values of which were measured as 0.26 and 0.14, respectively. The content of $ginsenoside-Rb_1$ was determined by HPLC on $Lichrosorb-NH_2$ column with $CH_3CN/H_2O/n-BuOH$(80 : 20 : 10, v/v). Its recovery rate in the extract granules, was as relatively low as $19.8{\pm}1.4%$ compared to the content in raw red ginseng.

• Korean Journal of Food Science and Technology
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• v.34 no.6
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• pp.1145-1148
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• 2002

Melatonin, which is a hormone secreted from pineal gland of brain and known to prevent oxidative damages of various tissues, was analyzed in 26 domestic edible plants. For the preparation of melatonin fraction, 50% ethanol extract prepared from lyophilized plant powder was filtered and applied on TLC plate. Melatonin position on TLC developed with acetone was identified by fluorescence light and extracted with methanol. This methanolic fraction was injected into HPLC comprising ODS-A column, fluorescence detector, and mobile phase consisting of a mixture (30 : 70, v/v) of 70% ammonium acetate and methanol at a flow rate of 1.0 mL/min. Melatonin was identified at the retention time of 17 min. Results revealed that celery, leek, broccoli, and cauliflower had higher melatonin contents than others.

• Journal of the Korean Society of Food Science and Nutrition
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• v.20 no.5
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• pp.473-478
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• 1991

After the glycolipid(GL) and phospolipid (PL) of flint corn during a growth process were fractionated by silicic acid column chromatography (SACC), the GL and PL of polar lipids were separated by thin layer chromatography (TLC)and quantitative by TLC scanner and the fatty acid composition in polar lipids were determined by gas chromatography (GC). Of the GL in polar lipids were separated by TLC, monoglycosyl diacylglycerol (8.3~29.2%) was the major component, and monoglycosyl ceramide, monoglycosyl sterol were also found as minor components. Of the PL, phosphatidyl choline (27.2~29.5%) and phosphatidyl inositol (42.9~79.1%) were the major components, and phosphatidyl ethanolamine and cardiolipin were also persont in the PL. The major fatty acids in the GL were oleic(27.1~37.1%), linoleic (13.2~35.7%) and palmitic (22.5~25.6%) acids. The major fatty acids in the PL were palmitic (46.5~52.3%), heptadecanoic (23.0~25.1%) and oleic(7.2~14.6%) and GL contained a higher percentage of unsa-turated fatty acids, but PL presented that of the saturated fatty acids.