• Title/Summary/Keyword: THICKNESS of LEAVES

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Assessment of anatomical characteristics of the medicinal plant African cherry (Prunus africana) for its accurate taxonomic identification

  • Komakech, Richard;Yang, Sungyu;Song, Jun Ho;Choi, Goya;Kim, Yong-Goo;Okello, Denis;Omujal, Francis;Kyeyune, Grace Nambatya;Matsabisa, Motlalepula Gilbert;Kang, Youngmin
    • Journal of Plant Biotechnology
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    • v.49 no.2
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    • pp.139-144
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    • 2022
  • The genus Prunus (family: Rosaceae) consists of over 400 plant species and exhibits vast biodiversity worldwide. Given the wide distribution of this genus, its taxonomic classification is important. Anatomical characteristics are conserved and stable and can therefore be used as an important tool for the taxonomic characterization of plants. Therefore, this study aimed to assess and document the anatomical characteristics of the leaf, stem, and seed of P. africana using micrographs and photographs for possible use in the identification, quality control, and phylogenetic analysis of the species. The anatomical sections of a young stem revealed a cortex consisting of isodiametric parenchyma cells, druse crystals, primary vascular bundles, and pith. The mature stem bark majorly consisted of the rhytidome, with the periderm densely arranged in multiple layers; a cluster of stone cells; and sclerenchyma. The leaf sections were hypostomatic, with stomata sizes ranging from 18.90-(22.34)-26.90 × 15.41-(18.40)-21.22 ㎛. The leaf sections showed the presence of characteristic druse crystals, vascular bundles, and mesophyll layers. The pericarp contained the epicarp, mesocarp, and endocarp, with their thickness being approximately 350-400, 300-350, and 30-50 ㎛, respectively. In addition, it contained a seed testa with a thickness of approximately 50-60 ㎛. The morphological and anatomical characteristics observed in P. africana leaves, stems, and seeds in this study could serve as useful data for the taxonomic identification of this species.

Impact of Transplant Timing of Mother Plants for Seedling Strawberries on Growth and Development of Daughter Plants and Initial Field Stage (딸기 육묘를 위한 모주의 정식시기가 자묘 소질 및 본포 초기생육에 미치는 영향)

  • Park, Gab-Soon;Kim, Young-Chil;Ann, Seoung-Won
    • Journal of Environmental Science International
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    • v.25 no.2
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    • pp.289-294
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    • 2016
  • In this study, we examined the impact of transplant timing of mother plants for seedling strawberry on growth and development of daughter plants and field stage. The leaf growth of treated mother plants and crown thickness were low when transplant was performed on April $10^{th}$. Based on the results collected until July $13^{th}$, the numbers of daughter plants with more than two leaves were 20.6 and 19.5 for March $10^{th}$ and March $25^{th}$, and these values decreased by April $10^{th}$ and April $25^{th}$ showing values of 15.1 and 11.8, respectively. After seedling growth was complete, leaf area and fresh weight of the saplings were remarkably low beginning from the transplant on April $10^{th}$. Crown thickness of saplings was generally lower when transplant timing was late. After 45 days of transplant leaf length and width were noticeably lower than the transplant on April $10^{th}$ and $25^{th}$. First cluster was 100% for both the March $10^{th}$ and $25^{th}$ transplant, followed April 10th and $25^{th}$ with values of 66% and 43%. The results revealed that transplant on March $10^{th}$ and $25^{th}$ had a greater positive impact on the growth and development of the strawberry cultivar 'Seolhyang'. A supplementary study will have to be conducted to determine the relationship between the harvest period of the first cluster and the yield of marketable strawberries depending on the transplanting time of mother plants.

Impact of Runner Development of Mother Plants during Seedling Strawberries on Daughter Plants Growth and Development during Phase of First Cluster (딸기 육묘과정 중 모주의 런너발생수가 자묘 소질과 1화방 수확기 생장에 미치는 영향)

  • Park, Gab-Soon;Ann, Seoung-Won
    • Journal of Environmental Science International
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    • v.25 no.1
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    • pp.147-153
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    • 2016
  • To investigate the impact of the number of runners developed from daughter plants during seedling on sapling growth and development during phase of first cluster, we examined four treatment groups that had 3-4, 5-6, 7-8, and 9-10 runners. As of June $5^{th}$, the group with 9-10 runners showed the highest number of saplings with more than 2 leaves, followed by groups with 7-8, 5-6, and 3-4 runners. Although observation on June $25^{th}$ and July $15^{th}$ showed a similar tendency for sapling numbers, no significant difference was detected between groups with 7-8 and 9-10 runners. The length of runners in the treated groups was similar to that in groups with 3-4 and 5-6 runners, with lengths of 49.4 mm and 48.0 mm, respectively, but runner length was significantly shorter in the group with 7-8 runners. Both the thickness and the weight of runners were highest in the group with 3-4 runners. The growth and development of daughter plants and root weight were similar between the groups with 3-4 and 5-6 runners, whereas both values were lower in groups with 7-8 and 9-10 runners. While stem crown thickness values were 8-7 mm and 8.5 mm in groups with 3-4 and 5-6 runners, respectively, groups with 7-8 and 9-10 runners had thinner stems of 7.1 mm and 6.2 mm, respectively. The fresh weight of saplings decreased as the number of runners increased. From the phase first cluster, leaf area and fresh weight were remarkably low in groups with 7-8 and 9-10 runners. Taken together, these results can be used to increase the production of high-quality saplings by providing a foundation for studies to investigate the impact of the number of runners developed from daughter plants on growth and development during the seedling period and the phase first cluster.

Effects of Lotus (Nelumbo nucifera) Leaf Hot Water Extracts on the Quality and Stability of Eggs using Ultrasonication Treatment during Storage

  • Lee, Jihye;Seo, Han Geuk;Lee, Chi-Ho
    • Food Science of Animal Resources
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    • v.40 no.6
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    • pp.1044-1054
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    • 2020
  • This study was performed to investigate the effects of lotus leaf hot water extracts treatment on the quality and stability of eggs using impregnation treatment through ultrasonication during storage. A total of 480 eggs were categorized into four treatment groups (n=30 each)-non-treated (CON), soaked for 30 min in lotus leaf hot water extracts without ultrasonication (T1), sonicated in distilled water (T2), and sonicated in lotus leaf hot water extracts (T3)-and stored for 15 d at 30℃. The egg weight, Haugh unit (HU), egg grade, albumen height, yolk color, eggshell thickness, eggshell breaking strength, and weight loss were measured for egg quality assessment. 2-Thiobarbituric acid reactive substance (TBARS) and volatile basic nitrogen (VBN) contents were measured as stability indicators. Additionally, total phenolic contents (TPC), total flavonoid contents (TFC), and 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical scavenging activity were evaluated. The HU, egg grade, albumen height, and yolk color of T3 were significantly higher than those of CON (p<0.05). No significant differences in eggshell thickness and eggshell breaking strength are observed among the groups. The weight loss of T3 was significantly lower than that of the other groups during storage (p<0.05). The application of lotus leaf hot water extracts also significantly reduced TBARS and VBN (p<0.05). The TPC, TFC, and DPPH radical scavenging activity of T3 were significantly higher than those of the other groups (p<0.05). These results suggest that lotus leaf hot water extracts may be useful as a natural ingredient for improving the quality and stability of eggs during storage.

Study on Wood Structure and Fiber Characteristics of Genus Lespedeza species (싸리나무류재(類材)의 조직(組織)과 섬유(纖維)에 관(關)한 연구(硏究))

  • Kim, Su-In;Yang, Chang-Sul
    • Journal of the Korean Wood Science and Technology
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    • v.16 no.1
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    • pp.9-20
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    • 1988
  • Lespedeza species have been widely used not only as plant resources for bark, leaves and honey, but also as erosion control materials. This study was carried out to investigate the structural and wood tiber characteristics in order to obtain basic information for the utilization of the wood. The wood structure was investigated for 10 selected species and the wood fiber, for the 5 selected species among Lespedeza species distributed all over the country. The following results were obtained. In the cross section, campylotropis showed ray diffuse porous wood, L. bicolor, L. cyrtobotrya, L. thunbergii var. intermedia, diffuse porous wood, and L. tomentella, L. angustifolioides. L. maritima, L. robusta, L. patentibicolor, ring porous wood. The maximum diameter of a single vessel ranged 66-123 ${\mu}$ in all species. Campylotropis showed the most number of vessels, L. angustifolioides, the least. The number of ray per mm ranged 7-22, Campylotropis showed the most number of rays, L. angustifolioides, the least. In the radial section the average length of vessel ranged 121-250 ${\mu}$ in all species. L. thunbergii var. intermedia showed the longest vessel, L. tomentella, the shortest. In tangential section the average width of the uniseriate ray ranged 9.2-14.7${\mu}$, that of the multiseriate ray, 19.2-42.1 ${\mu}$. The average height of the uniseriate ray ranged 143.0-1162.0 ${\mu}$. The width of fiber ranged 10.12-13.61 ${\mu}$, L. maximowiezii showed the narrowest tiber, L. thunbergii var. intermedia. the widest, the thickness of fiber wall ranged 2.93-3.71 ${\mu}$ in the five species. L. maximowiezii showed the most thin fiber wall, L. thunbergii var. intermedia, the thickest, L. cyrtobotrya showed the difference in the size of fiber between the shade and the sunny sites but L. maximowiezii showed no difference. There was significant difference in fiber length, fiber width and wall thickness between L. eyrtobotrya and L. maximowiezii.

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Morphological characterization of Korean and Turkish watermelon germplasm

  • Huh, Yun Chan;Choi, Hak Soon;Solmaz, Ilknur;Sari, Nebahat;Kim, Su
    • Korean Journal of Agricultural Science
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    • v.41 no.4
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    • pp.309-314
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    • 2014
  • A total of 67 watermelon accessions which include 37 accessions from Korean and 27 accessions from Turkish germplasm and 3 accessions of other related species from USA were investigated for morphological characteristics. The UPOV descriptor list for 56 characters (6 seedlings, 4 plants, 11 leaves, 5 flowers, 23 fruits and 7 seeds) was used in characterization. In addition, eight quantitative characters, hypocotyl length, cotyledon width, cotyledon length, fruit weight, fruit length, fruit width, thickness of outer layer of pericarp and soluble solid content were also measured. The 56 qualitatively scored characters were analyzed by principle coordinate analysis (PCoA) while the eight quantitative ones were subjected to principle component analysis (PCA). Morphological characterization result demonstrated that the accessions displayed high morphological diversity(how much percent?). A high level of phenotypic diversity was observed from the results of morphological characterization. However, plant growth habit and leaf blade flecking showed constant characters for all of the accessions. The Korean and Turkish watermelon genotypes are diverse groups and can be separated by both multivariate analysis of morphological characters although the grouping was more apparent in PCoS results.

Effect of Sucrose, Germicides and ABA in the Preservatives on Postharvest Quality of Cut Chrysanthemum 'Kyoungsubang` (보존용액에 함유된 당, 살균제 및 ABA가 절화국화의 수확후 품질에 미치는 영향)

  • 박윤영;김학윤;조문수
    • Journal of Life Science
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    • v.10 no.6
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    • pp.591-597
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    • 2000
  • An investigation was conducted to elucidate the effect of sucrose, germicides and abscisic acid (ABA) in the preservatives on postharvest quality, such as fresh weight, solution uptake, vase life, diameter and thickness of flower, and chlorolhyll of leaf in cut chrysanthemum 'Kyoungsubang`. Fresh weight of cut chrysanthemum was highest in 250mg/L aluminum sulfate [{TEX}$Al_{2}(SO_{4})_{3}${/TEX}]+3% sucrose and 250 mg/L 8-hydroxyquinoline sulfate (8-HQS)+ 3% sucrose. Addition of sucrose and germicides inhibited microorganisms growth in solution, and apparently promoted solution uptake. The increased fresh weight seems to attribute to the elevated uptake. Germicide 8-HQS was more effective than {TEX}$Al_{2}(SO_{4})_{3}${/TEX} in the aspect of solution uptake and fresh weight. Treatment of 250 mg/L 8-HQS +3% sucrose showed the longest vase life (24.67±2.52 days) and the lowest rate of leaf chlorosis (or senescence). Addition of {TEX}$Al_{2}(SO_{4})_{3}${/TEX} and sucrose accelerated leaf chlorosis and increased diameter of flower, respectively. Sucrose treatment prolonged the vase life of flowers. Except control and 250 mg/L 8-HQS, content of chlorophyll was rapidly decreased after 6 days at upper, middle and lower leaves in order.

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Understanding Bacterial Biofilm Stimulation Using Different Methods - a Criterion for Selecting Epiphytes by Plants

  • Bhushan, Shashi;Gogoi, Mandakini;Bora, Abhispa;Ghosh, Sourav;Barman, Sinchini;Biswas, Tethi;Sudarshan, Mathummal;Thakur, Ashoke Ranjan;Mukherjee, Indranil;Dey, Subrata Kumar;Chaudhuri, Shaon Ray
    • Microbiology and Biotechnology Letters
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    • v.47 no.2
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    • pp.303-309
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    • 2019
  • Earlier studies by our group revealed that gallic acid in phytochemicals stimulated biofilm production in epiphytes, while caffeic acid in phytochemicals inhibited biofilm production in non-epiphytes. It is well documented that antimicrobial secretion by some epiphytic bacteria inhibits non-epiphytic bacterial growth on leaf surfaces. These selection criteria help plants choose their microbial inhabitants. Calcium and iron in phytochemicals also stimulate biofilm formation and thus, may be selection criteria adopted by plants with respect to their native epiphytic population. Furthermore, the processing of leaves during phytochemical extraction impacts the composition of the extract, and therefore its ability to affect bacterial biofilm formation. Computation of the Hurst exponent using biofilm thickness data obtained from the Ellipsometry of Brewster Angle Microscopic (BAM) images is an efficient tool for understanding the impact of phytochemicals on epiphytic and non-epiphytic populations when compared to fluorescent microscopy, scanning electron microscopy, and staining techniques. To the best of our knowledge, this is the first report that uses the Hurst exponent to elucidate the mechanism involved in plant microbe interaction.

A study on the hydroponic cultivation of Chinese cabbage for kimchi (김치용 배추의 수경재배에 관한 연구)

  • 한덕철;문성원;김혜자;조재선
    • Korean journal of food and cookery science
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    • v.17 no.5
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    • pp.510-516
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    • 2001
  • Hydroponic cultivation is a technology of raising crops without use of soil. Generally farmers use the method of DFT(deep flow technology)to grow leafy or fruity vegetables; however, systematic and scientific researches are insufficient on this matter. This study investigated the possibility of cultivating Chinese cabbage steadily year long by using the method of DFT. Chinese cabbage was cultivated hydroponically with and without Ge addition, used to prepare kimchi, and the chemical and microbiological characteristics of kimchi were compared. The basic hydroponic cultivation condition was as follows: 30 days after seeding, the raised seeds were moved to a hydroponic bed and given underground water for 3 days so the roots grow normally Standard nutrient solution was provided and the early electric conductivity concentration was maintained between 1.5∼2.5 thickness. The temperature of the solution was maintained between 10 ∼25$^{\circ}C$ to allow the growth of Chinese cabbage. When soil-cultivated, organically cultivated and hydroponically cultivated Chinese cabbages were compared, hydroponically cultivated cabbages were smaller in size and showed less ability to build up and fold leaves into a head, but showed better quality than organically cultivated cabbages. The contents of protein and fat showed no significant differences. The contents of water. Ca, P, Fe, Vitamin A and Niacin were higher in control and Ge-added cabbages compared with soil-grown cabbage. There was no difference between soil-cultivated Chinese cabbage kimchi and hydroponically cultivated Chinese cabbage kimchi.

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Cold-hardiness Tetraploid Induced by Colchicine Treatment in Mulberry Seedings(Morus alba L. Yongchonppong/Kaeryanppong) (지배실생(용천뽕/개량뽕)의 생장점에 Colchicine 처리로 창성한 내동성계 4배체뽕)

  • 박광준
    • Journal of Sericultural and Entomological Science
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    • v.36 no.1
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    • pp.1-7
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    • 1994
  • Four tetraploid mulberry lines, Sawonppon No.11, No.13 and No.14, were induced by the colchicine treatment on the shoot tips of seedlings originated from the cross between Yongchonppong and Kaeryanppong. The major characteristics of the tetraploid lines were as follows: Green tip sprouting stage was similar to Kaeryanppong, a medium budding variety. Leaves were medium- to large-sized cordate type. Leaf surface was rougher and stronger than that of the diploid parents. Leaf thickness, leaf area weight and leaf water content were higher than those of the diploid parents. Average branch length was shorter than that of the diploid parents. Internode length and number of lateral branches were similar to the average values of the two parents. Death atop rate of branch was 1.6%~2.5% indicating strong cold-hardiness of the tetraploid lines. Therefore, these lines could be used as sources of cold-hardiness in developing triploid lines.

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