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Thidiazuron Induced High Frequency Adventitious Shoot Formation and Plant Regeneration in Capsicum annuum L.

  • VENKATAIAH PEDDABOINA;CHRISTOPHER THAMIDALA;SUBHASH KARAMPURI
    • Journal of Plant Biotechnology
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    • 제5권4호
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    • pp.245-250
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    • 2003
  • An efficient procedure was developed for adventitious shoot bud induction and plantlet regeneration from various explants of the ten genotypes of Pepper (Capsicum annuum L.) using Thidiazuron (TDZ). Among various treatments at 1.0-3.0 mg/L TDZ Induced maximum number of adventitious shoots depending upon the explant type and genotype compared to other treatments. Among the explants tested, leaf induced maximum number of adventitious shoots than the cotyledons. TDZ-mediated organo-genesis was possible in 10 pepper cultivars, the extent of the response being genotype-dependent. Of the ten genotypes tested, C. annuum cvs CA960, $G_4$ and X-235 were produced maximum number of adventitious shoots and Sell was the least, and all other genotypes gave moderate response. Elongation of multiple shoots was observed on medium supplemented with SA (0.05 mg/L) in combination of IAA (0.05 mg/L). Differences in ability for in vitro shoot regeneration and elongation depend upon the variety and explant type. The elongated shoots were success. Fully rooted on MS medium containing at 1.0 mG/L IAA. Plantlets regenerated from different explants of ten genotypes were found to be diploid (2n=24) and were devoid of any chromosomal aberrations. Regenerated plants were successfully established in soil where 85-90% of them developed into morphologically normal and fertile plants.

수종(數種) 근관소독제(根管消毒劑) 및 근관충전재(根管充塡材)의 세포독성(細胞毒性)에 관(關)한 실험적(實驗的) 연구(硏究) (Evaluation of the Cytotoxicity of Root Canal Disinfectants and Root Canal Sealers on L Cells in Vitro)

  • 정충모
    • Restorative Dentistry and Endodontics
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    • 제7권1호
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    • pp.85-99
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    • 1981
  • This study was to evaluate the cytotoxic effect of three root canal disinfectants (formocresol, camphorated phenol and eugenol) and ten root canal sealers(Cavitec, Hypo-cal, Vitapex, AH26, Canals, Mynol, $N_2$, $N_2$-Medical, Z. O. E. and Calvital) in vitro. The experiments were performed in four differrent modes. In the first and second experiment, the "long-distance" cytotoxicity of three root canal disinfectants were tested on L cells. In the third exeriment, ten root canal sealers were tested for cytotoxicity by means of the tissue culture-agar overlay method immediately, 4 and 24 hours after the experiment. In the fourth experiment, the study with radioactively labeled L cells were employed to determine the relative cytotoxicity of ten root canal sealers. The results were as follows; 1. Every vapors from disinfectants showed more or less cytotoxicity. Of the three disinfectats, formocresol appeared to be the highest cytotoxic effect and camphorated phenol was the lowest. 2. Root canal sealers tested in tbis study showed cytotoxicity at every stage of time intervals. 3. The highest cytotoxic effect was freshly mixed $N_2$ meaical and $N_2$ also revealed the highest cytotoxic effect after 4 or 24 hours among these materials. Vitapex was found the lowest cytotoxic effect at all experimental stage. 4. Root canal sealers except N2 and Mynol showed cytotoxic effect were decreased cytotoxicity according to the time elapsed.

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가속 열화 시험에 따른 저압용 차단기의 물리적 특성에 관한 연구 (A Study on the Physical Characteristics of the Low-voltage Circuit Breaker Based on the Accelerated Degradation Test)

  • 강신동;김재호
    • 한국안전학회지
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    • 제37권6호
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    • pp.1-8
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    • 2022
  • This study analyzed the characteristics of insulation resistance and operating time based on an accelerated degradation test of a low-voltage circuit breaker. The experimental sample used a molded case circuit breaker (MCCB) and an earth leakage circuit breaker (ELCB). After measuring the insulation resistance of the circuit breakers, the leakage current was affected by an external rather than an internal structure. Furthermore, the insulation resistance of the circuit breakers with accelerated degradation was measured using a Megger insulation tester. In the accelerated degradation test, aging times of five, ten, 15, and 20 years were applied according to a temperature derived using the Arrhenius equation. Circuit breakers with an equivalent life of ten, 15, and 20 years had increased insulation resistance compared to those with less degradation time. In particular, the circuit breaker with an equivalent life of ten years had the highest insulation resistance. Component analysis of the circuit breaker manufactured through an accelerated degradation test confirmed that the timing of the increase in insulation resistance and the time of additive loss were the same. Finally, after analyzing the operating time of the circuit breakers with degradation, it was confirmed that the MCCB did not change, but the ELCB breaker failed.

3D 프린팅 기술로 출력된 치과 보철물의 임상적 적합도 평가 (Evaluation of clinical adaptation of dental prostheses printed by 3-dimensional printing technology)

  • 김기백
    • 대한치과기공학회지
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    • 제41권1호
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    • pp.1-7
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    • 2019
  • Purpose: The objectives of this study was to evaluate clinical adaptation of dental prostheses printed by 3 dimensional(3D) printing technology. Methods: Ten study models were prepared. Ten specimens of experimental group were printed by 3D printing(3DP group). As a control group, 10 specimens were fabricated by casting method on the same models. Marginal gaps of all specimens were measured to evaluate clinical adaptation. Marginal adaptations were measured using silicone replica technique and measured at 8 sites per specimen. Wilcoxon's signed-ranks test was used for statistical analysis(${\alpha}=0.05$). Results: Means of marginal adaptations were $95.1{\mu}m$ for 3DP group and $75.9{\mu}m$ for CAST group(p < 0.000). Conclusion : However, the mean of the 3DP group was within the clinical tolerance suggested by the previous researchers. Based on this, dental prosthesis fabricated by 3D printing technology is considered to be clinically acceptable.

Gamma Knife Radiosurgery for Ten or More Brain Metastases

  • Kim, Chang-Hyun;Im, Yong-Seok;Nam, Do-Hyun;Park, Kwan;Kim, Jong-Hyun;Lee, Jung-Il
    • Journal of Korean Neurosurgical Society
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    • 제44권6호
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    • pp.358-363
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    • 2008
  • Objective : This study was performed to assess the efficacy of GKS in patients with ten or more brain metastases. Methods : From Aug 2002 to Dec 2007, twenty-six patients (13 men and 13 women) with ten or more cerebral metastatic lesions underwent GKS. The mean age was 55 years (32-80). All patients had Karnofsky performance status (KPS) score of 70 or better. According to recursive partitioning analysis (RPA) classification, 3 patients belonged to class I and 23 to class II. The location of primary tumor was lung (21), breast (3) and unknown (2). The mean number of the lesions per patient was 16.6 (10-37). The mean cumulated volume was 10.9 cc (1.0-42.2). The median marginal dose was 15 Gy (9-23). Overall survival and the prognostic factors for the survival were retrospectively analyzed by using Kaplan Meier method and univariate analysis. Results : Overall median survival from GKS was 34 weeks (8-199). Local control was possible for 79.5% of the lesions and control of all the lesions was possible in at least 14 patients (53.8%) until 6 months after GKS. New lesions appeared in 7 (26.9%) patients during the same period. At the last follow-up, 18 patients died; 6 (33.3%) from systemic causes, 10 (55.6%) from neurological causes, and 2 (11.1 %) from unknown causes. Synchronous onset in non-small cell lung cancer (p=0.007), high KPS score (${\geq}80$, p=0.029), and controlled primary disease (p=0.020) were favorable prognostic factors in univariate analysis. Conclusion : In carefully selected patients, GKS may be a treatment option for ten or more brain metastases.

CAD/CAM 밀링 시스템을 활용한 단일 치관과 3본 교의치의 3D 적합도 평가 (Three-dimensional evaluation of the internal adaptation of single and three-unit fixed dental restoration by CAD/CAM milling system)

  • 김소리;김총명;김웅철;김지환
    • 대한치과기공학회지
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    • 제39권1호
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    • pp.35-42
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    • 2017
  • Purpose: The purpose of this paper was to evaluate the occurrence of errors regarding adaptation by conducting a three-dimensional assessment comparing the bridge type dental restoration after the cutting process, which has multiple abutments, with a single type dental restoration. Methods: By using ten identical files obtained by scanning the master model, thirty designs were created consisting of ten maxillary right first premolars and ten maxillary right first molars with single crown abutments, along with ten bridge designs with the identical abutment. A 5-axis milling machine was used to produce the design file. The produced denture prostheses were scanned using a silicone replica for a STL file. An evaluation was conducted using 3D analysis software on the master model and each of the thirty data files. Results: The RMS value of the pre-molar (14) was $38.4{\pm}4{\mu}m$ for single and $54.7{\pm}6{\mu}m$ for bridge abutment; therefore, a statistically significant difference was observed for single and bridge designs although both shared the same abutment form (P<.05). Also, the RMS value of the molar (16) was $47.6{\pm}2{\mu}m$ and $56.6{\pm}5{\mu}m$ for the single and bridge designs, respectively, thereby presenting a statistically significant difference (P<.05). Conclusion: As a result, dental prosthesis fabricated using the single method presented better internal adaptation outcomes.

쪼그려 앉았다 일어나기 과제 수행 시 발생하는 전신 운동가변성의 발달적 변화 (The Age-Related Changes of Whole-Body Motor Variability during Sit-to-Stand Task)

  • Kim, Min Joo;Shim, Jae Kun
    • 한국운동역학회지
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    • 제32권3호
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    • pp.87-93
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    • 2022
  • Objective: The purpose of this research was to investigate the age-related changes in whole-body motor variability during sit-to-stand (STS) task. It has been reported that children perform motor tasks less accurately with greater variability as compared to adults. However, it is still unknown how they utilize the abundant degrees of freedom and accomplish voluntary actions. Uncontrolled manifold (UCM) analysis has been used to partition motor variabilities into two independent variability components, task-relevant variability (VORT) and task-irrelevant variability (VUCM). We investigated what differences exist between children and adults with respect to these two motor variability components in relation to motor development stages. Method: Ten 6-year-old children (height: 116.2 ± 4.3 cm, weight: 23.1 ± 3.9 kg, motor development assessment percentile score: 77.5 ± 18.6%), ten 10-year-old children (height: 138.7 ± 7.2 cm, weight: 35.8 ± 10.3 kg, motor development assessment percentile score: 73.9 ± 12.7%), and ten young adults (age: 23 ± 1.6 year-old, height: 164.3 ± 11.4 cm, weight: 60.8 ± 12.0 kg) participated in this study. Each participant performed STS ten times, and a motion capture system was used to capture the whole-body kinematics. Each segment centers of mass and the whole-body center of mass were calculated, and UCM analysis was used to quantify motor variabilities, VORT and VUCM. One-way ANOVA was used for statistical analysis. Results: We found that children produced more motor variabilities in VORT and VUCM in all three dimensions, anterior-posterior, medial-lateral, and vertical. As age increased, both, VORT and VUCM significantly decreased (p<.05). Conclusion: The greater VORT found in children compared to adults indicates that the repeatability over repetitions improves through development, while the greater VUCM found in children suggests that children better utilize the abundant degrees of freedom during STS compared to adults.

향 정신성약물의 장기투여가 흰쥐 장기에 미치는 영향 (The Effects of Chronic Administration of Psychotropic Drugs on Various Organs in Rats)

  • 김혜성
    • 대한약리학회지
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    • 제9권1호
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    • pp.23-37
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    • 1973
  • This paper presents the effect of chronic administration of psychotropic drugs on rats. The experimental animals were litter mates (average initial body weight $47{\pm}1.1g$) whose mother were bred at our laboratory. Each litter mate was treated as one group. Control animals were treated with tap water and each experimental group was treated with caffeine citrate 0.1%, nialamide 0.1%, ethyl alcohol 2.5%, phenobarbital sod. 0.1%, diphenylhydantoin 0.1%, chlorpromazine 0.1%, reserpine 0.005%, diazepam 0.01%, chlorpheniramine 0.01% solutions respectively in drinking water over a period of ten weeks. All rats were allowed food and drinking water ad libitum. The mortality rate and the per cent increase of body weight were recorded weekly throughout the course of the experiment. The effects of above agents on the pentobarbital sleeping time, gastric secretion, and brain and liver weights were studied at the end of ten weeks treatment. The obtained results are summarized as follows: 1. Mortality rate was highest in the groups treated with phenobarbital and chlorpromazine respectively. Through the experimental period (ten weeks), the mortality rate was higher in earlier stage than in the later period. 2. During the period of prolonged administration of psychotropic drugs, only diazepam treated group showed remarkable difference in per cent increase of body weight from the control group of rats. 3. Acute treatment with psychotropic drugs delayed the onset of pentobarbital sleeping time. In contrast, the sleeping time was significantly shortened (p<0.001) when the rats were treated chronically with those agents. 4. The effects of chronic treatment with phenobarbital or diphenylhydantoin on the gastric secretion are as follows: the total acidity was remarkably decreased while the pH was increased. 5. The brain weight was significantly decreased in the ethyl alchol and in the chlorpheniramine treated groups, in the mean time, there was no change in liver weight treated with any psychotropic drugs.

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한국산(韓國産) 작약근(芍藥根) 함유(含有) Paeoniflorin 과 Albiflorin의 분리(分離) 및 분석(分析) (Isolation and Determination of Paeoniflorin and Albiflorin in Korean Peony(Paeonia lactiflora Pall) Root)

  • 정명근;강광희
    • 한국약용작물학회지
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    • 제5권4호
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    • pp.249-254
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    • 1997
  • 한국산 재배작약(Paeonia lactiflora Pall.)에서 작약근의 주 활성물질로 평가되는 paeoniflorin과 albiflorin을 분리하였고. 국내 재배작약 10계통간에 paeoniflorin 과 albiflorin의 함량을 정량적으로 분석 한 결과를 요약하면 다음과 같다. 1. 한국산 재배작약으로 부터 silicagel column chromatography를 실시하여 paeoniflorin 과 albiflorin을 분리하였고, 그 화학적 구조를 분광학적 방법 $(UV,\;FT-IR,\;^1H{\cdot}^{13}C-NMR)$으로 확인 하였으며, HPLC 상대순도는 각각 98%, 93%였다. 2. 국내 재배작약 10계통의 paeoniflorin함량을 조사한 결과 $1.56%{\sim}4.04%$의 범위를 나타내었으며, 이들 중 의성 9호가 1.56%로 가장 낮았고, 밀양 4호가 4.04%로 가장 높은 양상을 나타내어 재배 계통간에 차이가 있음을 확인하였다. 3. 국내 재배작약 10계통의 albiflorin함량은 의성 4호가 0.04%로 가장 낮은 함량을 나타내었고, 풍기 27호가 1.98%로 가장 높아 계통간 차이가 뚜렸하였고, 조사된 10계통 중 풍기 계통은 $0.10%{\sim}1.98%$로 기타 계통보다 함량이 월등히 높았다.

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한국-EU 자유무역협정의 수산부문 무역효과 추정 (The Trade Effect of Korea-EU FTA on the Fishery Sector)

  • 김남두;황상인
    • 통상정보연구
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    • 제12권4호
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    • pp.49-65
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    • 2010
  • We have analyzed the trade effect of Korea-EU FTA on the Korean fishery sector, after reviewing the trade pattern and the tariff barriers of fishery sector between Korea-EU. For the trade effects, we have categorized into three cases: 1) the complete tariff elimination of all items, 2) the half tariff reduction on top ten valued items, with complete tariff elimination of other items, and 3) the complete tariff elimination, except unbinding top three valued items. The effect of the complete tariff elimination of all items implies the effect of the full achievement of FTA. For other two cases, these effects imply the effects of the transitional phenomenon of FTA since the complete tariff elimination happens gradually over more than ten years. For the complete elimination of tariff, we found that imports are increased by 1.1 billion dollars which is 12.9% increase in average imports during years 2006-2009. Also, exports are increased by 1.3 billion dollars which is 14.5% increase in average exports during same years.

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