• 생명과학회지
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• 제12권5호
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• pp.561-565
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• 2002

Pseudomonas sp. EL-041는 페놀로 적응된 활성오니로부터 분리되었으며, 페놀을 분해할 수 있는 동시에 trick-loroethylene(TCE)을 공동대사할 수 있는 세균이다. 본 연구에서는 Pseudomonas sp. EL-041에서 발현되는 trick-loroethylene 분해효소의 종류를 효소활성 측정 및 specific phenol oxygenase gene의 DNA sequencing을 통하여 조사하였으며, 그 결과 본 균주의 trichloroethylene 분해효소는 monooxygenase였으며, phenol hydroxylase로 추정되었다.

• 한국생물공학회:학술대회논문집
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• 한국생물공학회 2000년도 춘계학술발표대회
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• pp.370-373
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• 2000

In this work, an extractive membrane bioreactor containing coulture broth of Burkholderia cepacia G4 PR1 constitutively expressing the TCE-degrading enzyme, tolune-ortho-monooxygenase(TOM), was used for the degradation of TCE. The membrane bioreactor operates by seperating the TCE-containing waste gas from the aerated biomedium, by which the air-stripping of TCE without degradation was overcome that could occur in conventional aerobic biological treatments of TCE-contaminated waste gases. This was achieved by a silicone rubber membrane which was coiled around a perspex draft tube. TCE from the gas phase diffuses across the silicone rubber membrane into microbial culture broth that was continuously fed from a separate aerobic CSTR. Therefore, TCE degradation occured without the TCE being directly exposed to the aerating gas stream. Of the TCE supplied to the membrane bioreactor, 72.6% was biodegraded during the operation of this system. To construct a mathematical model for this system, parameters describing microbial growth kinetics on TCE were determined using a CSTR bioreactor. Else parameters used for numerical simulation were determined from either indepedent experiments or values reported in the literature. The model was compared with the experimental data, and there was a good agreement between the predicted and the measured TCE concentrations in the system. To achieve a higher treatment efficiency, various operating conditions were simulated as well.