• KSBB Journal
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• v.15 no.2
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• pp.195-200
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• 2000

Studies were made on the optimization of media to c비tivate Thraustochytrium aureum A TCC 34304 for the enhanc엉d p production of linoleic acid. The medium optimization was made with the artificial sea water medium. Yeast extract, sodium g glutamate, peptone and tryptone were considered as nitrogen source. The effect of $\infty$ncentration of nitrogen source as well a as initial glucose on the production of linoleic acid were investigated to optimize the media. The maximum yield of lipid was 0 0.302 mg/g cell mass when initial glucose $\infty$ncentration was 10 g/L and sodium glutamate was used as nitrogen source, a and the yield of linoleic acid to unit cell mass was also maximum to be 8 % in that case. The highest linoleic acid c concentration was obtained in the initial glucose concentration 30 g/L regardless of the kinds of nitrogen source and the | linoleic acid concentration was 0.208 g/L when peptone was supplemented to be 2 g/L.

• Asian Pacific Journal of Cancer Prevention
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• v.16 no.8
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• pp.3543-3549
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• 2015

Background: Bladder cancer is one of the most common cancers worldwide. Gene expression profiling using microarray technologies improves the understanding of cancer biology. The aim of this study was to determine the gene expression profile in Egyptian bladder cancer patients. Materials and Methods: Samples from 29 human bladder cancers and adjacent non-neoplastic tissues were analyzed by cDNA microarray, with hierarchical clustering and multidimensional analysis. Results: Five hundred and sixteen genes were differentially expressed of which SOS1, HDAC2, PLXNC1, GTSE1, ULK2, IRS2, ABCA12, TOP3A, HES1, and SRP68 genes were involved in 33 different pathways. The most frequently detected genes were: SOS1 in 20 different pathways; HDAC2 in 5 different pathways; IRS2 in 3 different pathways. There were 388 down-regulated genes. PLCB2 was involved in 11 different pathways, MDM2 in 9 pathways, FZD4 in 5 pathways, p15 and FGF12 in 4 pathways, POLE2 in 3 pathways, and MCM4 and POLR2E in 2 pathways. Thirty genes showed significant differences between transitional cell cancer (TCC) and squamous cell cancer (SCC) samples. Unsupervised cluster analysis of DNA microarray data revealed a clear distinction between low and high grade tumors. In addition 26 genes showed significant differences between low and high tumor stages, including fragile histidine triad, Ras and sialyltransferase 8 (alpha) and 16 showed significant differences between low and high tumor grades, like methionine adenosyl transferase II, beta. Conclusions: The present study identified some genes, that can be used as molecular biomarkers or target genes in Egyptian bladder cancer patients.

• Asian Pacific Journal of Cancer Prevention
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• v.14 no.2
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• pp.1131-1132
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• 2013

Background: Upper tract transitional cell carcinomas (UTCC) are relatively uncommon but prognosis is generally worse than TCC of bladder. Methods: Between March 2004 and June 2012, patients with initial non-metastatic UTCC were assessed in the Medical Oncology and Urology Departments of Ataturk Training and Research Hospital. Results: A total of 11 patients with initially non-metastatic UTCC were detected in the 8 year period, all males. Median age of was 62 (range, 38-74). Six lesions were located in the renal pelvis and 5 in the ureter. Nephroureterectomy was performed in 9 patients, and distal ureterectomy and cuff excision of the bladder in the remaining 2. The majority (n= 9) had high grade tumors. Median primary tumor diameter was 3.5 cm (range, 0.7-10). Five patients (45.5%) were stage I, 2 (18.2%) were stage II, and 4 (36.4%) were stage III. While adjuvant chemotherapy was not applied for stage I and II disease (n= 7), 4 to 6 courses were applied for 3 of the stage III patients. Also one stage III case received adjuvant radiotherapy. Up to 100 months follow-up, median overall survival was 13 months (range, 5-100 months). While stage I and II patients are following-up without muscle-invasive progression, 2 of stage III patients demonstrated progression. Conclusion: We need more collaborative studies to determine management of especially pT3-pT4 patients with UTCC.

• Korean Journal of Biological Psychiatry
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• v.5 no.2
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• pp.215-218
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• 1998

The $5-HT_{2A}$ receptor is of great interest for research into schizophrenia and psychopharmacology in light of the observation that schizophrenic patients has 5-HT cortical-subcortical imbalance and atypical antipsychotic clozpine has $5-HT_{2A}$ antagonists properties. An significant association between schizophrenia and the T102C polymorphism of the gene for $5-HT_{2A}$receptor has been reported. In this study, we investigated an association between schizophrenia and the T102C polymorphism of the gene for $5-HT_{2A}$ receptor in Korean schizophrenic patients. The subjects consisted of 139 schizophrenic patients and 88 normal controls. Genomic DNA was amplified by PCR and digested with MsPI. The uncutt product identified allele 1(nucleotide sequence TCT) ; digested products of 216bp and 156bp identified allele 2(nucleotide sequence TCC). The allele frequencies and the genotypic distribution of $5-HT_{2A}$ receptor gene were not significantly different between schizophrenic patients and normal controls. Since allele frequencies of the T102C polymorphism may differ between individuals of different ethnic backgrounds, it needs to be conducted in an advanced research.

• Korean Journal of Materials Research
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• v.17 no.11
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• pp.574-579
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• 2007

Amorphous $BaTi_4O_9$ ($BT_4$) film was deposited on Pt/Si substrate by RF magnetron sputter and their dielectric properties and electrical properties are investigated. A cross sectional SEM image and AFM image of the surface of the amorphous $BT_4$ film deposited at room temperature showed the film was grown well on the substrate. The amorphous $BT_4$ film had a large dielectric constant of 32, which is similar to that of the crystalline $BT_4$ film. The leakage current density of the $BT_4$ film was low and a Poole-Frenkel emission was suggested as the leakage current mechanism. A positive quadratic voltage coefficient of capacitance (VCC) was obtained for the $BT_4$ film with a thickness of <70 nm and it could be due to the free carrier relaxation. However, a negative quadratic VCC was obtained for the films with a thickness ${\geq}96nm$, possibly due to the dipolar relaxation. The 55 nm-thick $BT_4$ film had a high capacitance density of $5.1fF/{\mu}m^2$ with a low leakage current density of $11.6nA/cm^2$ at 2 V. Its quadratic and linear VCCs were $244ppm/V^2$ and -52 ppm/V, respectively, with a low temperature coefficient of capacitance of $961ppm/^{\circ}C$ at 100 kHz. These results confirmed the potential suitability of the amorphous $BT_4$ film for use as a high performance metal-insulator-metal (MIM) capacitor.

• Proceedings of the Korean Vacuum Society Conference
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• 2011.02a
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• pp.78-78
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• 2011

수열합성법을 이용하여 Si(111) 기판에 ZnO 박막을 성장하였다. ZnO 박막의 성장을 위한 씨앗층은 plasma-assisted molecular beam epitaxy (PA-MBE)를 이용하였다. 씨앗층의 표면 거칠기(root-mean-square roughness)는 2.5 nm이고, 씨앗층 위에 성장된 ZnO 박막은 다양한 크기의 입자들로 이루어져 있었으며 두께는 약 $1.8{\mu}m$로 매우 일정하였다. 배향성을 알아보기 위하여 texture coefficient (TC)를 계산해 보았다. TC(100)과 TC(200)은 a-축 배향성을, TC(002)는 c-축 배향성을 나타내는데, c-축으로 더 우세한 배향성(99.5%)을 보였다. TC 비율(TCa-axis/TCc-axis)은 열처리 온도를 $700^{\circ}C$까지 올렸을 때, 점차적으로 증가하였고, 그 이상의 열처리 온도(< $900^{\circ}C$)에서는 급격히 감소하였다. 잔류응력과 Zn와 O의 bond length도 유사한 경향을 보였다. $700^{\circ}C$까지 열처리 온도가 증가함에 따라, 잔류응력은 증가하였고 bond length는 감소하였다. Near-band-edge emission (NBE)의 피크 강도는 열처리 온도가 $700^{\circ}C$까지 증가함에 따라 점차적으로 증가하였다. 열처리 온도가 $800^{\circ}C$ 이상 증가함에 따라 deep-level emission (DLE)가 적색편이(red-shift)하였다. $700^{\circ}C$로 열처리를 한 ZnO 박막이 가장 우세한 (002)방향의 배향성을 보였을 뿐만 아니라 가장 큰 발광효율 증가를 보였다.

• Food Science of Animal Resources
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• v.40 no.6
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• pp.896-907
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• 2020

Imaging technique or computer vision (CV) technology has received huge attention as a rapid and non-destructive technique throughout the world for measuring quality attributes of agricultural products including meat and meat products. This study was conducted to test the ability of CV technology to predict the quality attributes of beef. Images were captured from longissimus dorsi muscle in beef at 24 h post-mortem. Traits evaluated were color value (L*, a*, b*), pH, drip loss, cooking loss, dry matter, moisture, crude protein, fat, ash, thiobarbituric acid reactive substance (TBARS), peroxide value (POV), free fatty acid (FFA), total coliform count (TCC), total viable count (TVC) and total yeast-mould count (TYMC). Images were analyzed using the Matlab software (R2015a). Different reference values were determined by physicochemical, proximate, biochemical and microbiological test. All determination were done in triplicate and the mean value was reported. Data analysis was carried out using the programme Statgraphics Centurion XVI. Calibration and validation model were fitted using the software Unscrambler X version 9.7. A higher correlation found in a* (r=0.65) and moisture (r=0.56) with 'a*' value obtained from image analysis and the highest calibration and prediction accuracy was found in lightness (r²_c=0.73, r²_p=0.69) in beef. Results of this work show that CV technology may be a useful tool for predicting meat quality traits in the laboratory and meat processing industries.

• Journal of Microbiology and Biotechnology
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• v.18 no.11
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• pp.1853-1857
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• 2008

Salmonella contamination in chicken meat was studied with 100 chicken meat samples purchased from 55 shops located in various regions. A total of 21 isolates of Salmonella enterica were isolated from 21 chicken meat samples from four shops located at open markets, whereas there were none from supermarkets with well-equipped cold systems. Among these, 18 isolates were identified as Salmonella enterica serotype Haardt (S. Haardt) and three isolates were S. enterica serotype Muenchen. When the minimal inhibitory concentrations of the S. Haardt isolates were assayed with the agar dilution method to determine susceptibility to ampicillin, chloramphenicol, sulfisoxazole, tetracycline, and nalidixic acid, all 18 isolates were resistant to tetracycline and nalidixic acid and nine of these were resistant to ampicillin. These isolates showed reduced susceptibility to eight fluoroquinolones including ciprofloxacin, enrofloxacin, levofloxacin, gatifloxacin, gemifloxacin, moxifloxacin, norfloxacin, and ofloxacin. When quinolone resistance determining regions of gyrA and gyrB were sequenced, every isolate had the same missense mutation Ser83$\rightarrow$Tyr (TCC$\rightarrow$+TAC) in gyrA, whereas no mutation was found in gyrB. Pulsed-field gel electrophoresis with XbaI revealed a close relationship among these isolates, suggesting a contamination of raw chicken meat with clonal spread of nalidixic acid-resistant and quinolone-reduced susceptibility S. Haardt in chickens. Results in this study show the importance of a well-equipped cold system and the prudent use of fluoroquinolone in chickens to prevent the occurrence of quinolone-resistant isolates.

• Proceedings of the Korea Water Resources Association Conference
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• 2016.05a
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• pp.7-7
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• 2016

인도네시아 산불에 의한 연무는 동남아시아 인접한 국가들에 있어서 심각한 환경문제 중 하나이다. 국제적으로 심각한 문제를 야기하는 인도네시아의 산불은 건조기에 강수량이 적게 내리는 극심한 가뭄 조건에서 발생한다. 건조기 강수량을 모니터링 하는 것은 산불 발생 가능성을 예측하기 위해 중요하지만 산불을 사전에 예방하고 영향을 최소화하기에는 부족하다. 따라서 산불에 대한 선제적 사전예방을 위해서는 수개월의 선행예측 기간을 갖는 조기경보 시스템이 절실하다. 따라서 본 연구는 인도네시아 산불에 대한 선제적 대응을 위한 강수량 예측시스템을 개발하고 예측성을 평가하여 동남아시아 지역의 화재 연무 조기경보 시스템의 시제품(Prototype)을 개발하는데 있다. 강수량 예측을 위해서 APEC 기후센터의 계절예측정보의 활용 정도에 따라서 4가지 서로 다른 방법을 통합하여 사용하였다. 예측정보 기반의 방법들로는 대상지역의 강수량 예측을 위해서 대상 지역 상공의 계절예측 강수자료를 보정을 통해 직접적으로 사용하는 SBC (Simple Bias Correction) 방법과 대상 지역 상공의 강수 예측자료를 사용하는 대신에 지역 강수량과 높은 상관 관계를 보이는 다른 지역의 대리변수를 예측인자로 사용하는 MWR (Moving Window Regression) 방법이 있다. 또한 예측자료의 사용 없이 과거자료 기반의 기후지수(Climate Index) 중에서 지체시간을 고려하여 지역 강수량과 높은 상관관계를 갖는 경우 예측에 활용하는 관측자료 기반의 CIR (Climate Index Regression) 방법과 예측기반 MWR과 관측기반의 CIR 방법에서 선정된 예측인자를 동시에 활용하는 ITR (Integrated Time Regression) 방법이 사용되었다. 장기 강수량 예측은 보르네오 섬의 4개 지역에서 3개월 이하의 선행예측기간에 대하여 0.5 이상의 TCC (Temporal Correlation Coefficient)의 값을 보여 양호한 예측성능을 보였다. 예측된 강수량 자료는 위성기반 관측 강수량 및 관측 탄소 배출량 관계에서 결정된 강수량의 임계값과의 비교를 통해 산불발생 가능성으로 환산하였다. 개발된 조기경보 시스템은 산불 발생에 가장 취약한 해당지역의 건조기(8월~10월) 강수량을 4월부터 예측해 산불 연무에 대한 조기경보를 수행한다. 개발된 화재 연무조기경보 시스템은 지속적인 개선을 통해 현장 실효성을 높여 동남아국가 정부의 화재 및 산림관리자들에게 보급함으로써 동남아의 화재 연무로 인한 환경문제 해결에 기여할 수 있으리라 판단된다.

• Journal of The Korean Society of Agricultural Engineers
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• v.61 no.1
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• pp.1-8
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• 2019

Developing reliable soil moisture prediction techniques at agricultural regions is a pivotal issue for sustaining stable crop productions. In this study, a physically-based SWAP(Soil-Water-Atmosphere-Plant) model was suggested to estimate soil moisture dynamics at the study sites. ROSETTA was also integrated to derive the soil hydraulic properties(${\alpha}$, n, ${\Theta}_r$, ${\Theta}_s$, $K_s$) as the input variables to SWAP based on the soil information(Sand, Silt and Clay-SSC, %). In order to predict the soil moisture dynamics in future, the mid-term TIGGIE(THORPEX Interactive Grand Global Ensemble) and long-term S2S(Subseasonal to Seasonal) weather forecasts were used, respectively. Our proposed approach was tested at the six study sites of RDA(Rural Development Administration). The estimated soil moisture values based on the SWAP model matched the measured data with the statistics of Root Mean Square Error(RMSE: 0.034~0.069) and Temporal Correlation Coefficient(TCC: 0.735~0.869) for validation. When we predicted the mid-/long-term soil moisture values using the TIGGE(0~15 days)/S2S(16~46 days) weather forecasts, the soil moisture estimates showed less variations during the TIGGE period while uncertainties were increased for the S2S period. Although uncertainties were relatively increased based on the increased leading time of S2S compared to those of TIGGE, these results supported the potential use of TIGGE/S2S forecasts in evaluating agricultural drought. Our proposed approach can be useful for efficient water resources management plans in hydrology, agriculture, etc.