• Title/Summary/Keyword: TBA method

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Antioxidant Effect of Fermented Salicornia herbacea L. Liquid with EM (Effective Microorganism) on Pork (돼지고기에 대한 EM(Effective Microorganism) 함초 발효액의 항산화 효과)

  • Han Seung-Kwan
    • Food Science of Animal Resources
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    • v.24 no.3
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    • pp.298-302
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    • 2004
  • The study was conducted to determine the antioxidant activity of EM (Effective Microorganism) fermented glasswort liquid for utilizing as a functional food material. Glasswort leaves by freezing-dried were showed the highest 20.19% contents, it was 20% salt contents of cooked salts. To compare antioxidant effects of glasswort plant parts (dried ground leaves, stems, and roots of glasswort) were exploited for investigation of antioxidant activity. Antioxidant activity for the samples was investigated by TBA (Thiobarbituric acid reactive method). EM fermented glasswort liquid from coastal region, the highest antioxidative activity showed in the oven-dried leaves at 14 days after storage. It was showed antioxidant effect more than 5.3 times than control. In conclusion, antioxidative effect of glasswort was apparently exhibited through measurement of TBARS (Thiobarbituric acid reactive substances).

Antioxidant Activity of Salad Vegetables Grown in Korea

  • Xin Zao;Song, Kyung-Bin;Kim, Mee-Ree
    • Preventive Nutrition and Food Science
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    • v.9 no.4
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    • pp.289-294
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    • 2004
  • The antioxidant activity of forty two kinds of salad vegetables grown in Korea was evaluated. Methanol extract of freeze-dried vegetable was assayed by radical scavenging activity using 1,2-diphenyl-2-picrylhydrazyl (DPPH) and Fe^{2+}$-catalyzed lipid peroxidation inhibition by TBA method. Total phenolics were determined colorimetrically using Folin-Ciocalteu reagent. The highest radical scavenging activity was expressed by perilla leaf, followed by dandelion leaf, red and green leafy lettuce, of which $IC_{50}$ was less than 0.10 mg/mL. Angelica leaf showed the highest inhibitory action for lipid peroxidation with $95\%$, and then dandelion leaf, water spinach, and perilla leaf inhibited over $80\%$. However, lettuce (Iceberg) and young Chinese cabbage exhibited the lowest antioxidant activity based on both assay methods. Highly positive correlations between antioxidative activities and total phenolics were observed (p < 0.001). The results suggested that salad vegetables, especially perilla leaf, leafy lettuce, dandelion or angelica, could be used for easily accessible sources of natural antioxidants.

Antioxidant Flavonoids and Chlorogenic Acid from the Leaves of Erobotrya japonica

  • Jung, Hyun-Ah;Park, Jong-Cheol;Chung, Hae-Young;Kim, Jong;Choi, Jae-Sue
    • Archives of Pharmacal Research
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    • v.22 no.2
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    • pp.213-218
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    • 1999
  • The antioxidant activity of Eriobotrya japonica was determined by measuring the radical scavenging effect on DPPH (1,1-diphenyl-2-picrylhydrazyl) radical and lipid peroxidation produced when mouse liver homogenate was exposed to the air at $37^{\circ}C$, using 2-thiobarbituric acid (TBA). The methanol extract and its factions of Eriobotrya japonica leaves showed strong antioxidant activity. The antioxidant activity of EtOAc and n-BuOH soluble fractions were stronger than the others, and were further purified by repeated silica gel, MCl gel CHP-20P, and Sephadex LH-20 column chromatography. Antioxidant chlorogenic acid, quercetin-3-sambubioside from n-BuOH fraction and methyl chlorogenate, kaempferol- and quercetin-3-rhamnosides, together with the inactive ursolic acid and$ 2{\alpha}$-hydroxyursolic acid from EtOAc fraction were isolated. Antioxidant flavonoids and chlorogenic acid also showed prominent inhibitory activity against free radical generation in dichlorofluorescein (DCF) method.

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Effect of High Pressure Processing on Freshness of Meat Products (육류가공품의 고압처리가 신선도에 미치는 영향 평가)

  • Hwang, Seong-Hee
    • Journal of Food Hygiene and Safety
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    • v.33 no.4
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    • pp.272-279
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    • 2018
  • The high pressure processing (HPP) is a technology which can preserve the quality of foods, such as the fresh taste, incense, texture, vitamin content, and so on, by minimizing the heating process. It does so by applying an instantaneous and uniform pressure that is the same as the water pressure that is 60 km deep in the sea. HPP is a technology that can inhibit food poisoning and spoilage caused by microorganisms and is currently an actively studied area. In this study, we investigated the effects of a high pressure treatment (0, 4, 6 min) on sliced ham, which is a typical meat product, at 600 MP a were tested for their effect on freshness. Moisture contents varied from 48 to 69%, salinity varied from 1.07 to 1.11%, and the pH decreased from 6.4~6.5 to 6.1~5.15. However, there was no difference between the control and treatment groups. General bacteria stored at $20^{\circ}C$ after hyper-pressure treatment were found to have no significant microorganisms in all groups until 4 weeks. but exceeded $10^5$ in control group and HPP 6 min treatment group from 5 weeks, At week 7, it was found to exceed $10^6$. The results indicate it was not possible to ingest food in the 4-and 6 minute treatment groups. Coliform was not observed in all groups despite observing for a total of 7 weeks at $20^{\circ}C$ weight test. VBN, a method used to determine the protein freshness of meat, showed a VBN value of less than 1 mg% until the fourth week and a value of 1 to 2 mg% after 5 weeks. The TBA was used as an index of the degree of fat acidosis in the meat tissues. The results showed it was below 0.18 mgMA / kg until the end of 7 weeks; this value was within the range for fresh meat, and there was no difference in treatment group. In this experiment, deformation of the packaging material did not occur and no swelling occurred due to the generation of gas. It is believed that the basic preservation effect was achieved only by blocking with the air due to the close contact of the packaging material.

Effect of Drying Air Velocity on the Quality of Salted and Dried Mullet Roe (영암어라인의 품질에 대한 건조풍속의 영향)

  • 조상준
    • Journal of the Korean Society of Food Science and Nutrition
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    • v.20 no.5
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    • pp.503-508
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    • 1991
  • The salted and dried mullet(Mugil Japonicus) roe had about 42% of protein and 41% of lipid content as major compoments. Therefore the lipid oxidation and the deterioration of protein occurred during drying and storage periods. In order to keep the good quality of the dried roe, acid, peroxide, thiobarbituric acid value and the contitutional amino acid were studied in the different drying air velocities at the conditions of 60% R.H. and $20^{\circ}C$ of drying air temperature. The optimum air velocity showed 2m/sec, which was excellently superior to the lipid and protein quality than those of other air velocities on the dried mullet roe. The drying period shortened from 20 days in the conventional processing method to 7 days in the modified processing method that had 2m/sec of drying air velocity. The drying rate curve had only a falling-rate drying period and not to have a constant-rate drying period.

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Ouality Characteristics of Frozen Maesil (Prunus mume Sieb. et Zucc.) according to Thawing Method (해동방법에 따른 냉동매실의 품질특성 변화)

  • Kwon, Dae-Jun;Kim, Mi-Hyang;Lee, Nan-Hee;Kwon, O-Jun;Son, Dong-Hwa;Choi, Ung-Kyu
    • Journal of the Korean Society of Food Culture
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    • v.21 no.4
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    • pp.426-432
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    • 2006
  • This study was conducted to investigate the changes in quality characteristics of frozen maesil according to thawing methods. The quality of maesil thawed in microwave oven was superior to those thaw in refrigerating temperature(5 $^{\circ}$) and in room temperature(25 $^{\circ}$). Drip loss of maesil thawed in microwave oven was 3.2${\pm}$0.2%. The total content of free sugars of maesil was 426.6 mg%, and 3% of them was decreased during thawing in microwave oven. The total content of organic acids was 5,297.2 mg%, and 2.5% of them was decreased during thawing in microwave oven. The total content of free amino acids was 281.4 mg%, and 2.1% of them was decreased during thawing in microwave oven. The principle ingredients of frozen maesil was stand for the lost contents of free sugar and a content loss of free organic acid and free amino acid were the fewest by thawing. Antioxidant effect for soybean oil and linoleic acid of maesil extract were expressd POV and TBA values. Antioxidative activity of fresh maesil extract was highest followed by maesil thawed in microwave oven, thawed in refreezing temperature (5$^{\circ}$)and room temperature (25$^{\circ}$)

Effects of Vacuum Cooling Followed by Water Spraying on the Quality of Precooked Skipjack Katsuwonus pelamis (진공분무 냉각에 의한 자숙 가다랑어(Katsuwonus pelamis)의 냉각 및 품질 특성에 관한 연구)

  • Lee, Tae-Hun;Koo, Jae-Geun
    • Korean Journal of Fisheries and Aquatic Sciences
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    • v.47 no.1
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    • pp.12-17
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    • 2014
  • The cooling of precooked skipjack Katsuwonus pelamis is a critical thermal process in tuna canning because it affects the quality and yield of the canned tuna, as well as productivity. The combined method of vacuum cooling followed by water spraying (VC-WS) was investigated to increase cooling rates, and prevent loss of yield of the precooked tuna during vacuum cooling. For VC-WS, the precooked skipjack was cooled to $30^{\circ}C$ by vacuum at 31 mmHg and then wetted by spraying water for 2 min. The effects of VC-WS on cooling times, cooling loss, color, texture and lipid oxidation of the precooked tuna were compared with conventional spray cooling (SC).The cooling times for precooked skipjack from $75^{\circ}C-30^{\circ}C$ were 11 min for VC-WS and 145 min for SC. The cooling losses were 1.7 % for VC-WS and 1.6 % for SC. Peroxide and thiobarbituric acid (TBA) values of VC-WS were lower than those of SC. The loin of the VC-WS-treated skipjack was brighter and harder than the SC loin, as indicated by higher lightness and hardness values. Based on these results, we believe that the VC-WS process could compensate for the cooling loss of vacuum cooling and minimize changes in quality that occur during cooling of precooked skipjack tuna.

Quality Properties of Seasoned-Dried Pacific Saury Treated with Liquid Smoke -4. Quality Stability of Seasoned-Dried Pacific Saury Treated with Liquid Smoke During Storage-

  • Park Sung-Young;Chung Yeon-Jung;Lee Young-Mi;Yoon Seong-Suk;Lee Jung-Suck;Cha Yong-Jin
    • Fisheries and Aquatic Sciences
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    • v.5 no.4
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    • pp.287-294
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    • 2002
  • As a series of studies on improving quality of seasoned-dried Pacific saury, storage stability of seasoned-dried saury treated with liquid smoke (T2 product) was examined during storage at ambient temperature $(19\pm5^{\circ}C)$, comparing with control (C, seasoning only) and T1 (treatment of $0.05\%$ Rosemary as a antioxidant). The histamine contents (15.33-32.21mg/100g) of 3 seasoned-dried products were much lower than tolerance limit of intake during 80 days of storage. The water activity of 3 seasoned-dried products was in range of 0.692­0.735. The pH of T2 showed relatively lower than the others during storage. The POV of T2 was slightly higher until 30 days, but was lower after 45 days compared with Tl. The TBA value and viable cell count of T2 were the lowest among the samples during storage. The color values of 3 seasoned-dried products were not significantly different during storage. The sensory scores of T2 was the highest than those of T1 and C during storage. Therefore, liquid smoking method was a useful technique to extend shelf-life and retain good sensory quality of seasoned-dried Pacific saury.

Screening for Antioxidant Activity of Edible Plants (식용식물의 항산화효과 검색)

  • 조세연;한용봉;신국현
    • Journal of the Korean Society of Food Science and Nutrition
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    • v.30 no.1
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    • pp.133-137
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    • 2001
  • The antioxidant activitiesof methanol extracts of twenty one vegetables were tested using the method of 1,1-diphenyl-2-picryl hydrazyl (DPPH) reactivity and TBARS substance assay in vitro. The methanol extracts of the leaves from three plants such as Aster scaber, Chrysanthemum coronarium, Petasites japonicus were found to be th most effective on DPPH radical scavenging activity. The next effective ones were the leaves of Capsicum annuum, the peels of Ipomoea batatas and Solanum tuberosum, the stems and leaves of Ipomoea batatas, the levels of Solanum melongena, the fruits of Solanum melongena, the green fruits (seed) of Capsicum ammuum in order, and the others did not show a considerable activity. When tested on lipid peroxidation using a lipid peroxidation generation system mediated by $H_{2}O_{2}/Fe^{2+}$ in rat liver homogenates. the peels of Cucumis melo, the leaves and the fruits of Solanum melongena, of Solanum melongena, The levels of Aster scaber and Chrysanthemum coronarium, the stems and leaves of Ipomoea batatas and the peels of Ipomoea batatas were found to exhibit a good protective activity against lipid peroxidatian in the order of effectiveness.

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Antioxidative Effects of Scutellariae Radix Aaquaacupuncture Solution on Lipid Peroxidation Induced by Free Radicals (자유기에 의한 지질과산화 반응에 대한 황금 약침액의 항산화 효능)

  • Kim Sung-Il;Moon Jin-Young;Kim Kap-Sung;Kim Doo-Hie;Nam Kyung-Soo;Lim Jong-Kook
    • Journal of Society of Preventive Korean Medicine
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    • v.1 no.1
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    • pp.48-54
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    • 1997
  • Scutellariae radix, has been used as a natural drug for fever, inflammation, cataract, and liver disease in traditional medicine. This study was performed in order to investigate the antioxidative effects of Scutellariae radix aqua-acupuncture solution (SRAS) on lipid peroxidation by free radicals. Lipid peroxidation levels were determined by TBA method during the autoxidation of linoleic acid. In this linoleic acid autoxidation system, SRAS markedly exhibited antioxidant activity, which inhibited 89% of linoleic acid peroxidation. SRAS showed scavenging effects on ${\alpha},{\alpha}-diphenyl-{\beta}-picrylhydrazyl$(DPPH) radical, inhibited superoxide generation in xanthine-xathine oxidase system, and also inhibited lipid peroxidation of rat liver tissue by hydroxyl radical derived from $H_2O_2-FE^{+2}$ system. These effects were similar to those of $dl-{\alpha}-tocopherol$, BHA and BHT. In addition, SRAS protected the cell death induced by ter-butyl hydroperoxide (t-BHP) and significantly increased cell viability in the normal rat liver cell (Ac2F). On the basis of these results, it is suggested that SRAS might play a protective role in lipid peroxidation by free radicals.

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