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Ginsenoside Rg4 Enhances the Inductive Effects of Human Dermal Papilla Spheres on Hair Growth Via the AKT/GSK-3β/β-Catenin Signaling Pathway

  • Lee, Yun Hee;Choi, Hui-Ji;Kim, Ji Yea;Kim, Ji-Eun;Lee, Jee-Hyun;Cho, So-Hyun;Yun, Mi-Young;An, Sungkwan;Song, Gyu Yong;Bae, Seunghee
    • Journal of Microbiology and Biotechnology
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    • v.31 no.7
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    • pp.933-941
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    • 2021

  • Ginsenoside Rg4 is a rare ginsenoside that is naturally found in ginseng, and exhibits a wide range of biological activities including antioxidant and anti-inflammatory properties in several cell types. The purpose of this study was to use an in vivo model of hair follicle (HF)-mimic based on a human dermal papilla (DP) spheroid system prepared by three-dimensional (3D) culture and to investigate the effect of Rg4 on the hair-inductive properties of DP cells. Treatment of the DP spheroids with Rg4 (20 to 50 ㎍/ml) significantly increased the viability and size of the DP spheres in a dose-dependent manner. Rg4 also increased the mRNA and protein expression of DP signature genes that are related to hair growth including ALP, BMP2, and VCAN in the DP spheres. Analysis of the signaling molecules and luciferase reporter assays further revealed that Rg4 induces the activation of phosphoinositide 3-kinase (PI3K)/AKT and the inhibitory phosphorylation of GSK3β, which activates the WNT/β-catenin signaling pathway. These results correlated with not only the increased nuclear translocation of β-catenin following the treatment of the DP spheres with Rg4 but also the significant elevation of mRNA expression of the downstream target genes of the WNT/β-catenin pathway including WNT5A, β-catenin, and LEF1. In conclusion, these results demonstrated that ginsenoside Rg4 promotes the hair-inductive properties of DP cells by activating the AKT/GSK3β/β-catenin signaling pathway in DP spheres, suggesting that Rg4 could be a potential natural therapy for hair growth.

  • https://doi.org/10.4014/jmb.2101.01032 인용 PDF KSCI

An Investigation on German High Speed Railway Slab Track (독일 고속철도 슬래브궤도의 고찰)

  • 강보순
    • Proceedings of the KSR Conference
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    • 2001.10a
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    • pp.508-513
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    • 2001

  • State of the German high speed railway slab track were investigated. The Permanent Way consisting of rails and sleepers laid on ballast is a technically and economically viable solution. After two decades of targeted research and development activities Slab Track turned out to be a reasonable option on special fields.

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Discovery of New Proteinase Inhibitor for the Treatment of Osteoporosis

  • 손문호
    • Proceedings of the Korean Society of Applied Pharmacology
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    • 2001.04a
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    • pp.89-99
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    • 2001

  • ■ Cathepsin K is a attractive target for selectively and efficiently modulating the osteoclastic bone resorption. ■ OST-1857 is a lead compound which is specifically targeted to cathepsin K and showed efficacy in TPTX rats. ■ OST-compounds are in process of the preclinical study, joined by Yuhan research center.

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