• Title/Summary/Keyword: T-M interaction

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Association between a M-Iacking mutant D75N of pharaonis phoborhodopsin and its transducer is stronger than the complex of the wild-type pigment: Implication of the signal transduction

  • Sudo, Yuki;Iwamoto, Masayuki;Shimono, Kazumi;Kamo, Naoki
    • Journal of Photoscience
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    • v.9 no.2
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    • pp.314-316
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    • 2002
  • In halobacterial membrane, pharaonis phoborhodopsin (or pharaonis sensory rhdopsin II, psRII) forms a complex with its transducer pHtrII. Flash-photolyis of D75N mutant did not yield M-intermediate but an O-like intermediate is observed. We examined the interaction between D75N of ppR and t-Htr (truncated pHtrII). These formed a complex in the presence of n-dodecyl-$\beta$-D-maltoside, and the association accelerated the decay of the 0 of D75N from 15 to 56 s$\^$-1/. From the decay time constants under varying ratios of D75N and t-Htr, n, the molar ratio of D75N/t-Htr in the complex, and K$\_$D/, the dissociation constant, were estimated. The value of n was unity and K$\_$D/ was estimated to 146 nM. This K$\_$D/ value can be considered as the association between the photo-intermediate and t-Htr, which is deduced by the method of estimation. Previously we (Photochem. Photobiol. 74, 489-494 (2001)) reported K$\_$D/ of 15 $\mu$M for the interaction between the wild-type and t-Htr by means of the change of M-decay rates. Therefore, this value should be the K$\_$D/ value for the interaction between M of the wild-type and t-Htr.

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벤젠 이합체와 그 치환체의 양자역학을 통한 π-π interaction의 계산

  • Jo, Ji-Seong;Cho, Art.
    • Proceeding of EDISON Challenge
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    • 2014.03a
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    • pp.385-397
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    • 2014
  • 신약을 개발하거나 단백질 구조를 예측하는데 Molecular Mechanics (MM)의 방법을 사용한다. 하지만, MM 만으로는 자연현상에서 일어나는 결과를 정확하게 기술하기 어렵다. 본 연구는 기존의 MM 방법으로는 정확히 예측이 불가능한 비 공유결합 중 하나인 ${\pi}-{\pi}$ interaction을 양자역학 계산을 통해 정확한 예측이 가능한지 보았다. ${\pi}-{\pi}$ interaction 이란 생체 내, 의약 화합물에서 발견되는 결합이기 때문에, 단백질과 결합하는 구조의 예측이 중요하다고 할 수 있다. 본 실험은 ${\pi}-{\pi}$ interaction을 갖는 Sandwich, T shape, 그리고 Parallel displaced 세 가지 모형을 가지고 양자역학 계산을 수행하였다. 양자역학 계산은 DFT의 세가지 함수 M06_2X, M05_2X, B3LYP를 이용하였다. 실험결과에서 세 가지 함수가 각기 다른 결과를 보였는데, M06_2X의 결과에서 ${\pi}-{\pi}$ interaction을 더 정확하게 계산하였다. 이러한 결과를 바탕으로, 양자역학의 방법을 통해 MM에서는 예측이 불가능한 ${\pi}-{\pi}$ interaction을 계산 할 수 있고 이 부분을 고려하여 화합물 간의 결합구조를 예측을 향상시킬 수 있다.

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The Effect of Infant Massage on Mother-Infant Play Interaction (영아마사지가 유희적 모아상호작용에 미치는 효과)

  • 최소영;김영혜
    • Journal of Korean Academy of Nursing
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    • v.32 no.6
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    • pp.823-831
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    • 2002
  • This study is attempts to clarify the effect of infant massage for the promotion of primipara's mother-infant interaction Method: The term for collecting data for experimental group ranged from April 25, 2001 to June 5, 2001. The infants for this group were sampled among normal mother-infant from one postpartum care center located in J city. The term for collecting data for control group ranged from June 10, 2001 to August 3, 2001. The infants for this group were sampled among normal mothers infant from 1 general hospital, 1 university hospital and 1 postpartum care center located in J city. The experiment was implemented giving primipara education about massage based on protocol for infant massage provided by Johnson & Johnson Korea and they received 10 days of education, 10 minutes a day (from 10 to 11 a.m) In the post test, we videotaped both the control group and the experimental group visiting their homes 4 weeks after delivery to observe mother-infant play interaction. Data analysis was done using SAS and the homogeneity between general properties owned by both control group and experimental group and mother's perception scale for children was verified through -test. Mother-infant play interaction with both control group and experimental group was analyzed through t-test in the experiment. And analysis of mother-infant interaction points based on general properties was made using ANOVA and t-test. Result: Hypothesis that mother-infant play interaction with primipara who gave her infant a massage will be more active than that of the primipara who didn't was verified (t= -4.27, p=.0001). And the points in each item, points in each item were estimated as follows. Mother behavioral items (t=-4.96, p=.0001), infant behavioral item (t=-0.36, p=.71), mother-infant interaction reciprocity (t=-2.64, p=.01). Conclusion: An infant massage program can contribute to promoting the Mother-Infant Play Interaction positively.

Weak Normal Shock Wave/Turbulent Boundary Layer Interaction in a Supersonic Nozzle(1st Report, Time-Mean Flow Characteristics) (초음속 노즐에서의 약한 수직충격파와 난류경계층의 간섭(제1편, 시간적평균 흐름의 특성))

  • Hong, Jong-Woo
    • Journal of the Korean Society of Industry Convergence
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    • v.2 no.2
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    • pp.115-124
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    • 1999
  • The interaction of weak normal shock wave with turbulent boundary layer in a supersonic nozzle was investigated experimentally by wall static pressure measurements and by schlieren optical observations. The lime-mean flow in the interaction region was classified into four patterns according to the ratio of the pressure $p_k$ at the first kink point in the pressure distribution of the interaction region to the pressure $p_1$ just upstream of the shock. It is shown for any flow pattern that the wall static pressure rise near the shock foot can be described by the "free interaction" which is defined by Chapman et al. The ratio of the triple point height $h_t$ of the bifurcated shock to the undisturbed boundary layer thickness ${\delta}_1$ upstream of the interaction increases with the upstream Mach number $M_1$, and for a fixed $M_1$, the normalized triple point height $h_t/{\delta}_1$ decreases with increasing ${\delta}_1/h$, where h is the duct half-height.

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Transverse and longitudinal partial interaction in composite bolted side-plated reinforced-concrete beams

  • Oehlers, D.J.;Nguyen, N.T.;Ahmed, M.;Bradford, M.A.
    • Structural Engineering and Mechanics
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    • v.5 no.5
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    • pp.553-563
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    • 1997
  • A procedure is being developed for bolting plates to the sides of existing reinforced concrete beams to strengthen and stiffen them. Unlike standard composite steel and concrete beams in which there is longitudinal-partial-interaction at the steel/concrete interface (that is slip along the length of the beam), composite bolted side-plated reinforced-concrete beams are unique in that they also exhibit transverse-partial-interaction, that is slip transverse to the length of the beam. In this work, the fundamental mathematical models for transverse-partial-interaction and its interaction with longitudinal-partial-interaction are developed. The fundamental models are then further developed to determine the number of connectors required to resist the transverse forces and to limit the degree of transverse-partial-interaction in bolted side-plated reinforced concrete beams.

DFT 방법을 이용한 벤젠 삼합체 π-π interaction의 양자역학 계산

  • Jeong, Hyeon-Su;Park, Gi-Cheol;Cho, Art.
    • Proceeding of EDISON Challenge
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    • 2014.03a
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    • pp.399-408
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    • 2014
  • 신약을 개발하거나 단백질 구조를 예측하는데 Molecular Mechanics (MM)의 방법을 사용한다. 하지만, MM 만으로는 자연현상에서 일어나는 결과를 정확하게 기술하기 어렵다. 본 연구는 기존의 MM 방법으로는 정확히 예측이 불가능한 비 공유결합 중 하나인 ${\pi}-{\pi}$ interaction을 양자역학 계산을 통해 정확한 예측이 가능한지 알아보았다. ${\pi}-{\pi}$ interaction이란 생채 내, 의약 화합물에서 발견되는 결합이기 때문에, 단백질과 결합하는 구조의 예측에 중요하다고 할 수 있다. 본 실험은 ${\pi}-{\pi}$ interaction을 갖는 Sandwich, T shape, 그리고 Parallel displaced 세 가지 모형과 각각의 모형 아래에 분자를 하나 더 쌓은 모형을 추가하여 양자역학 재산을 수행하였다. 양자역학 계산은 DFT의 세가지 함수 M06_2X, M05_2X, B3LYP를 이용하였다. 실험결과에서 세 가지 함수가 각기 다른 결과를 보였는데, 상대적으로 B3LYP의 경우에는 세가지 모델에서 모두 제대로 된 에너지 변화를 계산하지 못하였으며, M06_2X와 M05_2X의 결과에서는 거리에 따른 ${\pi}-{\pi}$ interaction 에너지의 변화를 정확하게 계산하였다. 이러한 결과를 바탕으로, 양자역학의 방법을 통해 MM에서는 예측이 불가능한 ${\pi}-{\pi}$ interaction을 계산 할 수 있고 이 부분을 고려하여 화합물 간의 결합구조를 예측을 향상시킬 수 있다.

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Miscibility and Properties of cis-Polybutadiene/Ethyl-Branched Polyethylene Blends (II)

  • Cho, Ur-Ryong
    • Macromolecular Research
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    • v.8 no.2
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    • pp.66-72
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    • 2000
  • Cis-Polybutadiene (cis-PBD) and the three polyethylenes (PE's) having different branch content were mixed to investigate crystallinity, thermodynamic interaction parameter(c), and diluents effect. Crys-tallinty of PE's decreased with increasing content of amorphous cis-PBD because of a decrease in both the degree of annealing and kinetics of diffusion of the crystallizable polymer content. The thermodynamic interaction parameter(c) for the three blend systems approximately equals to zero near the melting point. These systems were determined to be miscible on a molecular scale near or above the crystalline melting point of the crystalline PE's. From the measurement of T$\sub$m/ vs. T$\sub$c/ behavior, all the three blends showed a straight line for a plot of T$\sub$m/ vs. T$\sub$c/. This result means that the melting behavior of PE is mainly due to a diluent effect of cis-PBD component.

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Experimental study on circular concrete filled steel tubes with and without shear connectors

  • Chithira, K.;Baskar, K.
    • Steel and Composite Structures
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    • v.16 no.1
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    • pp.97-114
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    • 2014
  • This paper deals with a study on ultimate strength behaviour of eccentrically loaded CFT columns with and without shear connectors. Thirty specimens are subjected to experimental investigation under eccentric loading condition. P-M curves are generated for all the test specimens and critical eccentricities are evaluated. Three different D/t ratios such as 21, 25 and 29 and L/D ratios varying from 5 to 20 are considered as experimental parameters. Six specimens of bare steel tubes as reference specimens, twelve specimens of CFT columns without shear connectors and twelve specimens of CFT columns with shear connectors, in total thirty specimens are tested. The P-M values at the ultimate failure load of experimental study are found to be well agreed with the results of the proposed P-M interaction model. The load-deflection and load-strain behaviour of the experimental column specimens are presented. The behaviour of the CFT columns with and without shear connectors is compared. Experimental results indicate that the percentage increase in load carrying capacity of CFT columns with shear connectors compared to the ordinary CFT columns is found to be insignificant with a value ranging from 6% to 13%. However, the ductility factor of columns with shear connectors exhibit higher values than that of the CFT columns without shear connectors. This paper presents the proposed P-M interaction model and experimental results under varying parameters such as D/t and L/D ratios.

Evaluation of interfacial tension for poly(methyl methacrylate) and polystyrene by rheological measurements and interaction parameter of the two polymers

  • Sung, Y.T.;Seo, W.J.;Kim, Y.H.;Lee, H.S.;Kim, W.N.
    • Korea-Australia Rheology Journal
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    • v.16 no.3
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    • pp.135-140
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    • 2004
  • Morphological and rheological properties of the poly(methyl methacrylate) (PMMA) and polystyrene (PS) blends were studied by scanning electron microscopy (SEM) and advanced rheometric expansion system (ARES). From the SEM results, the PMMA-PS blends showed dispersed morphology and the particle size of the dispersed phase was quite small (0.1~0.6 $\mu\textrm{m}$ compared with other immiscible polymer blends. Values of the interfacial tension of the PMMA-PS blend were obtained from the Choi-Schowalter and the Palierne emulsion models using the storage modulus of the PMMA and PS, and found to be 1.0 and 2.0 mN/m, respectively. The interfacial tension between the PMMA and PS was also calculated from the Flory-Huggins polymer-polymer interaction parameter ($\chi$) and found to be from 0.98 to 1.86 mN/m depending on the molecular weight and composition. Comparing the values of the interfacial tension from the Flory-Huggins polymer-polymer interaction parameter and the values measured by oscillatory rheometer, it is suggested that the interfacial tension of the PMMA-PS blend obtained from the polymer-polymer interaction parameter are in good agreement with the values obtained by rheological measurements.