• Gut and Liver
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• 제12권6호
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• pp.641-647
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• 2018

Background/Aims: Helicobacter pylori eradication rates are decreasing because of increases in clarithromycin resistance. Thus, finding an easy and accurate method of detecting clarithromycin resistance is important. Methods: We evaluated 70 H. pylori isolates from Korean patients. Dual-labeled peptide nucleic acid (PNA) probes were designed to detect resistance associated with point mutations in 23S ribosomal ribonucleic acid gene domain V (A2142G, A2143G, and T2182C). Data were analyzed by probe-based fluorescence melting curve analysis based on probe-target dissociation temperatures and compared with Sanger sequencing. Results: Among 70 H. pylori isolates, 0, 16, and 58 isolates contained A2142G, A2143G, and T2182C mutations, respectively. PNA probe-based analysis exhibited 100.0% positive predictive values for A2142G and A2143G and a 98.3% positive predictive value for T2182C. PNA probe-based analysis results correlated with 98.6% of Sanger sequencing results (${\kappa}$-value=0.990; standard error, 0.010). Conclusions: H. pylori clarithromycin resistance can be easily and accurately assessed by dual-labeled PNA probe-based melting curve analysis if probes are used based on the appropriate resistance-related mutations. This method is fast, simple, accurate, and adaptable for clinical samples. It may help clinicians choose a precise eradication regimen.

• 대한설비공학회:학술대회논문집
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• 대한설비공학회 2008년도 동계학술발표대회 논문집
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• pp.39-44
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• 2008

The heat exchange between the Borehole Heat Exchanger(BHE) and the surrounding ground depends directly on ground thermal conductivity k at the certain site. The k is thus a key parameter in designing BHE and coupled geothermal heat pump systems. Currently, although a thermal hydraulic response test(TRT) is mostly used in practice, the thermal hydraulic TRT needs additional power and is generally time-consuming. A new, simple wireless P/T probe for a hi-speed k determination was introduced in this paper. This technique using a wireless P/T probe is less time-consuming and requires no external source of energy for measurement and predicts local thermal properties by measuring soil temperatures along the depth. Measured temperature data along the depth was analyzed. In order to verify the new technique for the determination of ground thermal conductivity, ground thermal conductivity k that calculated from the measured temperature data using a wireless P/T probe was compared with one obtained from conventional hydraulic TRT. When comparing the average k of two methods, the relative error was approximately 10%. As a result, the electronic TRT can replace the conventional hydraulic TRT method after carrying out the additional research on a lot of sites.

• 한국소음진동공학회:학술대회논문집
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• 한국소음진동공학회 2014년도 추계학술대회 논문집
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• pp.358-360
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• 2014

For the several decades, various nanomaterials are broadly used in industry and research. With the growth of nanotechnology, the study of nanotoxicity is being accelerated. Particularly, mercury ion is widely used in real life. Because the mercury is representative high toxic material, it is highly recommended to detect the mercury ion. In previous reported work, thymine-thymine mismatches (T-T) capture mercury ion and create very stable base pair ($T-Hg^{2+}-T$). Here, we performed the high sensitive sensing method for direct label free detection of mercury ions and DNA binding using Kelvin Probe Force Microscope (KPFM). In this method, 30 base pairs of thymine (T-30) is used for mercury specific DNA binding ($T-Hg^{2+}-T$). KPFM is able to detect the mercury ion because there is difference between bare T-30 DNA and mercury mediated DNA ($T-Hg^{2+}-T$).

• 한국지반신소재학회논문집
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• 제12권2호
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• pp.1-11
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• 2013

지하매설구조물인 상수도관 주변 지반의 간극수 동결로 상수도관의 변형에 영향을 미칠 수 있다. 이에 최근에는 현장 계측을 통한 포장 하부구조체의 함수비를 주기적으로 측정하기 위해서 미국 Campbell사의 TDR(time domain reflectometry) CS616 함수량계와 독일의 IMKO사 제품의 TRIME FM를 이용한 수동 함수량계 TRIME P3, T3 Probe를 사용하고 있다. 일반적으로 TDR 함수량계와 TRIME 수동 함수량계는 흙의 종류, 입도, 다짐도, 온도 등에 의해 오차가 유발될 수 있기 때문에, 현장의 시료를 사용하여 TDR 함수량계의 실내보정실험을 반드시 수행해야 한다. 즉, TDR함수량계의 매뉴얼에서는 일반적인 흙의 보정방정식을 제안하고 있지만 현장 적용시에는 반드시 보정실험을 수행하도록 권고하고 있다. 본 연구에서는 상수관 등 지하매설구조물의 주변의 다양한 채움재료를 사용하여 실내 보정 실험을 수행하였으며, 이 결과로부터 TDR함수량계인 CS616, TRIME-T3, TRIME-P3의 보정모델식을 제안하였으며, 보정식에 대한 현장검증을 수행하였다.

• 한국심리학회지:법
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• 제11권1호
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• pp.21-36
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• 2020

P300 숨긴정보검사에서는 조사대상자가 거짓을 말하고 있는지 판단하기 위하여 관련자극의 P300 진폭이 무관련자극의 P300 진폭보다 통계적으로 유의하게 더 큰지를 평가한다. 구체적인 통계적 방법으로 독립표본 t 검증 또는 부트스트랩 방법을 사용할 수 있다. Rosenfeld와 Soskins, Bosh, Ryan(2004)은 "개인 내에서 관련자극과 무관련자극의 P300 평균을 비교하는데 사용하기에는 t 검증이 너무 둔감하다."면서 부트스트랩 방법을 사용하였다. 본 연구의 목적은 P300 숨긴정보검사에서 t 검증의 검증력이 부트스트랩 방법보다 더 낮은지 평가하는 것이다. 이를 위하여 39명의 실험참가자로부터 측정한 뇌파자료를 이용하여 Monte Carlo 연구를 수행하였다. 연구결과, t 검증과 백분위를 이용한 부트스트랩 방법의 1 종 오류율은 서로 비슷하였으며, 백분위를 이용한 부트스트랩 방법의 검증력이 t 검증의 검증력보다 약간 더 높았다. 두 검증 방법의 1 종 오류율은 모두 유의수준보다 낮은 값을 보였으며, 검증력은 이론적인 t 검증의 검증력보다 약간 낮은 값을 보였다. 반면에 표준오차를 이용한 부트스트랩 방법의 1 종 오류율과 검증력은 이론적인 t 검증의 1 종 오류율 및 검증력과 비슷한 값을 보였으며, t 검증의 검증력보다 실험조건에 따라 .012 ~ .081 더 높았다.

• 한국초전도학회:학술대회논문집
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• 한국초전도학회 2009년도 Korea Superconductivity Society Meeting 2009
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• pp.76-76
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• 2009

• 미생물학회지
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• 제21권4호
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• pp.229-237
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• 1983

Aspergillus nidulans의 tRNA 유전자의 구성과 발현기착을 연구하기 위하여 우선 Aspergillus의 총 tRNA 유전자를 cloning 하였다. Aspergillus의 핵 DNA롱 포자로 부터 분리해 내고 본질 형성에서 분리한 BamHI과 T4 DNA ligase를 사용하여 pBR322플라스미드에 재조합시켜서 cloning하였다. 15벤의 transformation을 하여 30,000개 의 transformants 얻었고, 이 중 Aspergillus DNA를 가지고 있는 colony는 5,300켜개였다. In vivo에 서 S2p로 표지 된 total tRNA를 probe로 하여 colony hybridization 실험 결과, 105개의 total tRNA유전자 clone을 얻었다. 위의 결과와 cohybridization 실험 결과를 분석해 보면, Asprgillus의 tRNA 유전자는 yeast의 그것보다는 좀 더 밀집되어 존재한다고 생각된다.

• 농업과학연구
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• 제42권3호
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• pp.183-189
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• 2015

Plant root penetration through soil profile is restricted by compacted layer such as plow pan under conventional tillage. For detecting the compact layer, we made a graduated T-shape probe and measured compared between the depths with rapid change in feeling hardness of hand penetration using T-shape probe and with a rapid increase of penetrometer cone index. On upland crops, including red pepper, corn, soybean and cucumber, plow pan depth ranged from 10 cm to 25 cm depth. The effective rooting depth (ER) had significant correlation with the plow pan depth (PP) except soils with the shallow ground water and/or poorly drained soil. The regression equation was ER = 0.9*PP ($R^2=0.54^{**}$, N = 14) with the applicative PP range of 10-25 cm.

• Progress in Superconductivity
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• 제13권3호
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• pp.169-177
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• 2012

Distribution of the local properties in GdBCO and YBCO coated conductors was investigated using Low-temperature Scanning Laser and Hall Probe Microscopy (LTSLHPM). We prepared GdBCO and YBCO coated conductors to study the spatial distribution of the current density in a single bridge. Inhomogeneity of the ${T_c}^{max}$ in the bridge was analyzed from experimental results of Scanning Laser Microscopy (SLM) near the superconducting transition. The local transport and screening current in the bridge were also investigated using Scanning Hall Probe Microscopy (SHPM). A series of line scans of SLM of the GdBCO and YBCO sample showed that lines with more inhomogeneous distributions of ${\delta}V$ had more inhomogeneous distributions of ${T_c}^{max}$. The defect of the superconducting layer of the GdBCO sample caused by damage to the substrate affected the current flow. And we could analyze the redistribution of the current density using SLM and SHPM.

• Journal of Microbiology
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• 제35권4호
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• pp.334-340
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• 1997

A collection of 132 temperature sensitive (ts) mutants was generated by the chemical mutagenesis of Schizosaccharomyces pombe wild type strain and screened for tRNA splicing defects on Northern blots by hybridization with an oligonucleotide that recognizes the exon of the S. pombe tRNA^Val as a probe. We identidied 6 mutants which accumulate precursor $tRNA^{Val}$. Among them, 2 mutants exhibited remarkable morphological differences compared to wild type cells. One tRNA splicing mutant showed elongated cell shape in permissive as well as non-permissive cultures. The other mutant exhibited shortened cell morphology only in nonpermissive culture. The total RNA pattern in the splicing mutants appeared to be normal. Genetic analysis of four $tRNA^{Val}$ splicing mutants demonstrated that the mutation reside in different genes.