• Gut and Liver
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• v.12 no.6
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• pp.641-647
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• 2018

Background/Aims: Helicobacter pylori eradication rates are decreasing because of increases in clarithromycin resistance. Thus, finding an easy and accurate method of detecting clarithromycin resistance is important. Methods: We evaluated 70 H. pylori isolates from Korean patients. Dual-labeled peptide nucleic acid (PNA) probes were designed to detect resistance associated with point mutations in 23S ribosomal ribonucleic acid gene domain V (A2142G, A2143G, and T2182C). Data were analyzed by probe-based fluorescence melting curve analysis based on probe-target dissociation temperatures and compared with Sanger sequencing. Results: Among 70 H. pylori isolates, 0, 16, and 58 isolates contained A2142G, A2143G, and T2182C mutations, respectively. PNA probe-based analysis exhibited 100.0% positive predictive values for A2142G and A2143G and a 98.3% positive predictive value for T2182C. PNA probe-based analysis results correlated with 98.6% of Sanger sequencing results (${\kappa}$-value=0.990; standard error, 0.010). Conclusions: H. pylori clarithromycin resistance can be easily and accurately assessed by dual-labeled PNA probe-based melting curve analysis if probes are used based on the appropriate resistance-related mutations. This method is fast, simple, accurate, and adaptable for clinical samples. It may help clinicians choose a precise eradication regimen.

• Proceedings of the SAREK Conference
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• 2008.11a
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• pp.39-44
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• 2008

The heat exchange between the Borehole Heat Exchanger(BHE) and the surrounding ground depends directly on ground thermal conductivity k at the certain site. The k is thus a key parameter in designing BHE and coupled geothermal heat pump systems. Currently, although a thermal hydraulic response test(TRT) is mostly used in practice, the thermal hydraulic TRT needs additional power and is generally time-consuming. A new, simple wireless P/T probe for a hi-speed k determination was introduced in this paper. This technique using a wireless P/T probe is less time-consuming and requires no external source of energy for measurement and predicts local thermal properties by measuring soil temperatures along the depth. Measured temperature data along the depth was analyzed. In order to verify the new technique for the determination of ground thermal conductivity, ground thermal conductivity k that calculated from the measured temperature data using a wireless P/T probe was compared with one obtained from conventional hydraulic TRT. When comparing the average k of two methods, the relative error was approximately 10%. As a result, the electronic TRT can replace the conventional hydraulic TRT method after carrying out the additional research on a lot of sites.

• Proceedings of the Korean Society for Noise and Vibration Engineering Conference
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• 2014.10a
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• pp.358-360
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• 2014

For the several decades, various nanomaterials are broadly used in industry and research. With the growth of nanotechnology, the study of nanotoxicity is being accelerated. Particularly, mercury ion is widely used in real life. Because the mercury is representative high toxic material, it is highly recommended to detect the mercury ion. In previous reported work, thymine-thymine mismatches (T-T) capture mercury ion and create very stable base pair ($T-Hg^{2+}-T$). Here, we performed the high sensitive sensing method for direct label free detection of mercury ions and DNA binding using Kelvin Probe Force Microscope (KPFM). In this method, 30 base pairs of thymine (T-30) is used for mercury specific DNA binding ($T-Hg^{2+}-T$). KPFM is able to detect the mercury ion because there is difference between bare T-30 DNA and mercury mediated DNA ($T-Hg^{2+}-T$).

• Journal of the Korean Geosynthetics Society
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• v.12 no.2
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• pp.1-11
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• 2013

Water content of subgrade soil in water supply systems has a large effect on performance. Many researchers lately make use of time-domain reflectometry (TDR) probes to measure the soil water content of subgrade soil from monitoring. The laboratory calibration test of TDR probe should be performed with soil field, because TDR probe can cause an error by type, gradation, density, and temperature of soil. This study shows the laboratory calibration test using TDR CS616, TDR-P3, TDR-T3. The calibration equations of TDR were then proposed. It was confirmed from the study that the data of TDR probe monitored in field could be used to estimate the freezing, unfrozen water content, and matric suction of soil.

• Korean Journal of Forensic Psychology
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• v.11 no.1
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• pp.21-36
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• 2020

In P300-based concealed information test (P300 CIT), it evaluates whether the P300 amplitude for the probe is significantly greater than that of the irrelevant to determine if the suspect is telling a lie. An independent sample t-test or a bootstrap method can be used as a statistical test to make that decision. Rosenfeld et al. (2004) used the bootstrap method, claiming that "t tests on single sweeps are too insensitive to use to compare mean probe and irrelevant P300s within individuals" and their method has been accepted to date. The purpose of the study is to evaluate whether the power of t-test is lower than that of the bootstrap method in the P300 CIT. The Monte Carlo study was conducted by using EEG collected from 39 participants. The results showed that the type I error rates of the t-test and the percentile bootstrap method were similar and the power of the percentile bootstrap method was slightly higher than that of the t-test. The type I error rates of the t-test and the percentile bootstrap method were slightly lower than the significance level and the powers of the two tests were also slightly lower than that of the theoretical t-test. On the other hand, the type I error rate and power of the standard error Bootstrap method were the same as those of the theoretical t-test and its power was .012 ~ .081 higher than that of t-test depending on experimental conditions.

• 한국초전도학회:학술대회논문집
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• 2009.07a
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• pp.76-76
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• 2009

• Korean Journal of Microbiology
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• v.21 no.4
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• pp.229-237
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• 1983

Total tRNA genes from Aspergillus nidulans were cloned for the further investigation of the structure and expression of Aspergillus tRNA genes. Aspergillus DNA was isolated from spores and cloned into pBR322 plasmid DNA using BamHI and $T_4$ ligase. The recombinant hybrid DNA was transformed into E. coli HB101 and some 30,000 transformants were initially selected. Of these, about 5,300 E. coli clones containing Aspergillus DNA inserted into plasmid pBR322 at BamHl site have been isolated. The hybridization data obtained from the labeled Aspergillus $^{32}P-tRNA$ indicated that 105 colonies carried the total tRNA genes. From the data above and cohybridization experiment, tRNA genes of Aspergillus nidulans seem to be twice more clustered than those of yeast.

• Korean Journal of Agricultural Science
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• v.42 no.3
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• pp.183-189
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• 2015

Plant root penetration through soil profile is restricted by compacted layer such as plow pan under conventional tillage. For detecting the compact layer, we made a graduated T-shape probe and measured compared between the depths with rapid change in feeling hardness of hand penetration using T-shape probe and with a rapid increase of penetrometer cone index. On upland crops, including red pepper, corn, soybean and cucumber, plow pan depth ranged from 10 cm to 25 cm depth. The effective rooting depth (ER) had significant correlation with the plow pan depth (PP) except soils with the shallow ground water and/or poorly drained soil. The regression equation was ER = 0.9*PP ($R^2=0.54^{**}$, N = 14) with the applicative PP range of 10-25 cm.

• Progress in Superconductivity
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• v.13 no.3
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• pp.169-177
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• 2012

Distribution of the local properties in GdBCO and YBCO coated conductors was investigated using Low-temperature Scanning Laser and Hall Probe Microscopy (LTSLHPM). We prepared GdBCO and YBCO coated conductors to study the spatial distribution of the current density in a single bridge. Inhomogeneity of the ${T_c}^{max}$ in the bridge was analyzed from experimental results of Scanning Laser Microscopy (SLM) near the superconducting transition. The local transport and screening current in the bridge were also investigated using Scanning Hall Probe Microscopy (SHPM). A series of line scans of SLM of the GdBCO and YBCO sample showed that lines with more inhomogeneous distributions of ${\delta}V$ had more inhomogeneous distributions of ${T_c}^{max}$. The defect of the superconducting layer of the GdBCO sample caused by damage to the substrate affected the current flow. And we could analyze the redistribution of the current density using SLM and SHPM.

• Journal of Microbiology
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• v.35 no.4
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• pp.334-340
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• 1997

A collection of 132 temperature sensitive (ts) mutants was generated by the chemical mutagenesis of Schizosaccharomyces pombe wild type strain and screened for tRNA splicing defects on Northern blots by hybridization with an oligonucleotide that recognizes the exon of the S. pombe tRNA^Val as a probe. We identidied 6 mutants which accumulate precursor $tRNA^{Val}$. Among them, 2 mutants exhibited remarkable morphological differences compared to wild type cells. One tRNA splicing mutant showed elongated cell shape in permissive as well as non-permissive cultures. The other mutant exhibited shortened cell morphology only in nonpermissive culture. The total RNA pattern in the splicing mutants appeared to be normal. Genetic analysis of four $tRNA^{Val}$ splicing mutants demonstrated that the mutation reside in different genes.