• Title/Summary/Keyword: T/B ratio

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Establishment of Culture System of a New Strain NT0423 of Bacillus thuringiensis (새로운 Bacillus thuringiensis NT0423 균주의 배양체계)

  • 김호산;노종열;이대원;우수동;강석권
    • Korean journal of applied entomology
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    • v.37 no.2
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    • pp.187-191
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    • 1998
  • For efficient and economical production of Btrc,illus tlz~rr.ingi~r1~sstirsa in NT0423 as amicrobial-control agents, a new culture medium and culture condition were developed. Five mediadesignatzd as SWI I , SW14, SW23. SW32 and SW4I were prepared ~ : i t hv arious mixture ratio ofsoybean cake and wheat bran. It was founcl that in terms of the cell growth rate and development ofsporulation of B, thrri.il~girrl.sis strain NT0423 in all SW culture media were more efficient than those inGYS and in LB media. Total cell number in all SW media showed similar values, hut SW32 lnediilm wasthe most efficient in the development of spore, which amo~~ntetod 3.7 x 10XC FUImI. Also. at the pHranging frorn 6.2 to 7.3 in the mediiun~ no ad\:erse effect was not made in the culture of B. thur-ingicnsisstrain NT0423. The optimal volume (%) of SW32 mecliuni in a 5 1 fernientor was determined as 4 8\rolume of total niediuni. resulting ill growth (4.2 x 1OTCFUlrnl) of H. t1~~irir1,yirrz.ssit.vr ain NT0423. As H.t l i ~ t r i t ~ g iw~ a~s~ csuil~tu rcd in the shakc-flash and 5 1 fcrnientor. bacterial cells were yielded to 1 X 10"CFUIml and 5 x I O1oCFLJlml.FUIml and 5 x I O1oCFLJlml.

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An Experimental study on the behavior of gap N-joints in Cold-formed Square Hollow Sections with connection plate for a tension member (인장용 연결 플레이트를 갖는 각형강관 갭 N형 접합부의 거동에 관한 실험적 연구)

  • Park, Keum Sung;Bae, Kyu Woong;Moon, Tae Sup
    • Journal of Korean Society of Steel Construction
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    • v.16 no.6 s.73
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    • pp.769-780
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    • 2004
  • This paper's objective is to evaluate the experimental behavior of gap N-joints made of cold-formed, square, hollow steel sections, with a connection plate as a tension member. The principal parameters for testing included the ratio of chord width to thickness, the ratio of brace width to chord width, eccentric ratio, the shape of the compression member, the branch angle, and the stiffening plate of the chord flange. The strength and failure mode were examined through the test for the gap N-joint, consisting of several parameters. Based on the results of the test, the gap N-joints were determined according to the capacity preceding the displacement of the tension, regardless of the width ratio, and the split failure mode-connected surface for a chord in joints. The strength of the gap N-joints increased proportionally as the $2\gamma$(B/T) ratio decreased, and as the width ratio(${\beta}$) of branch to chord increased. Particularly, $2\gamma$(B/T) decreased as the capacity of gap N-joint increased. The results of the test were summarized for the capacity, initial stiffness, ductility, and change of the failure mode of each gap N-joint.

A Proposal of Parallel Interworking Model for Broadband Access Network (광대역 액세스 망을 위한 병렬형 연동 모델의 제안)

  • 김춘희;차영욱;한기준
    • Journal of Korea Multimedia Society
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    • v.4 no.5
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    • pp.455-464
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    • 2001
  • For future multimedia services, one of the most crucial issues is building an access network that can accommodate multimedia services in subscriber network. The VB5.2 interface of B-ISDN located between an access network and a service node allows dynamic allocation and release of ATM resources. The SG 13 of ITU-T is standardizing the B-BCC protocol, which is sequentially interworked with signaling protocols in the service node. To minimize a connection setup delay of the sequential interworking mode, we proposed the parallel interworking model in which the SN executes simultaneously the connection control protocol of VB5.2 interface and signaling protocol. We simulate two interworking models in terms of a connection setup delay and a completion ratio. The results of simulation show that our proposed parallel interworking model for the VB5.2 interface reduces the setup delay and has the similar completion ratio compared to the sequential interworking model. however, the connection setup delay of parallel interworking model becomes about seven tenths of that of the sequential interworking model and the improvement become larger as the arrival rate increased.

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Tumor Size as a Prognostic Factor in Gastric Cancer Patient

  • Im, Won Jin;Kim, Min Gyu;Ha, Tae Kyung;Kwon, Sung Joon
    • Journal of Gastric Cancer
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    • v.12 no.3
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    • pp.164-172
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    • 2012
  • Purpose: The purpose of this study is to investigate the prognostic significance of tumor size for 5-year survival rate in patients with gastric cancer. Materials and Methods: A total of 1,697 patients with gastric cancer, who underwent potentially curative gastrectomy, were evaluated. Patients were divided into 4 groups as follows, according to the median size of early and advanced gastric cancer, respectively: small early gastric cancer (tumor size ${\leq}3$ cm), large early gastric cancer (tumor size >3 cm), small advanced gastric cancer (tumor size ${\leq}$ 6 cm), and large advanced gastric cancer (tumor size >6 cm). The prognostic value of tumor size for 5-year survival rate was investigated. Results: In a univariate analysis, tumor size is a significant prognostic factor in advanced gastric cancer, but not in early gastric cancer. Multivariate analysis showed that tumor size is an independent prognostic factor for 5-year survival rate in advanced gastric cancer (P=0.003, hazard ratio=1.372, 95% confidence interval=1.115~1.690). When advanced gastric cancer is subdivided into 2 groups, according to serosa invasion: Group 1; serosa negative (T2 and T3, 7th AJCC), and Group 2; serosa positive (T4a and T4b, 7th AJCC), tumor size is an independent prognostic factor in Group 1 (P=0.011, hazard ratio=1.810, 95% confidence interval=1.149~2.852) and in Group 2 (P=0.033, hazard ratio=1.288, 95% confidence interval=1.020~1.627), respectively. Conclusions: Tumor size is an independent prognostic factor in advanced gastric cancer irrespective of the serosa invasion, but not in early gastric cancer.

Analysis of C/N Variation of Ku Band Satellite Beacon Receiver According to Rain Attenuation (강우 감쇠에 따른 Ku 대역 위성 비콘 수신기 C/N 변화 해석)

  • Park, Dae-Kil;Lee, Kyung-Soon;Koo, Kyung Heon
    • Journal of Advanced Navigation Technology
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    • v.22 no.5
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    • pp.415-419
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    • 2018
  • This paper predicts and measures the C/N ratio of a beacon signal transmitted from geostationary orbit satellite KorSat 5A ($113^{\circ}E$) at a ground station located in Kimpo. Based on the ground stations, we compared the rain attenuation of the zone K of ITU-R and the rain attenuation which analyzed the domestic weather information. In ITU-R, the Korean rainfall characteristics are classified into zone K, but forecasting the rainfall intensity and attenuation of three adjacent cities based on the cumulative rainfall data per minute from 2013 to 2017. The calculation of rainfall path and attenuation is based on ITU-R recommendations. The change of the C/N according to the rainfall amount was confirmed through the 2 week satellite beacon signal C/N measurement. The predicted critical C/N was decreased to 12 dB at $A_{0.3}$. During the experiment, it was confirmed that it decreased up to 8 dB according to the concentrated rainfall.

Profiling Bartonella infection and its associated risk factors in shelter cats in Malaysia

  • Nurul Najwa Ainaa Alias;Sharina Omar;Nur Indah Ahmad;Malaika Watanabe;Sun Tee Tay;Nor Azlina Aziz;Farina Mustaffa-Kamal
    • Journal of Veterinary Science
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    • v.24 no.3
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    • pp.38.1-38.12
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    • 2023
  • Background: Poor disease management and irregular vector control could predispose sheltered animals to disease such as feline Bartonella infection, a vector-borne zoonotic disease primarily caused by Bartonella henselae. Objectives: This study investigated the status of Bartonella infection in cats from eight (n = 8) shelters by molecular and serological approaches, profiling the CD4:CD8 ratio and the risk factors associated with Bartonella infection in shelter cats. Methods: Bartonella deoxyribonucleic acid (DNA) was detected through polymerase chain reaction (PCR) targeting 16S-23S rRNA internal transcribed spacer gene, followed by DNA sequencing. Bartonella IgM and IgG antibody titre, CD4 and CD8 profiles were detected using indirect immunofluorescence assay and flow cytometric analysis, respectively. Results: B. henselae was detected through PCR and sequencing in 1.0% (1/101) oral swab and 2.0% (1/50) cat fleas, while another 3/50 cat fleas carried B. clarridgeiae. Only 18/101 cats were seronegative against B. henselae, whereas 30.7% (31/101) cats were positive for both IgM and IgG, 8% (18/101) cats had IgM, and 33.7% (34/101) cats had IgG antibody only. None of the eight shelters sampled had Bartonella antibody-free cats. Although abnormal CD4:CD8 ratio was observed in 48/83 seropositive cats, flea infestation was the only significant risk factor observed in this study. Conclusions: The present study provides the first comparison on the Bartonella spp. antigen, antibody status and CD4:CD8 ratio among shelter cats. The high B. henselae seropositivity among shelter cats presumably due to significant flea infestation triggers an alarm of whether the infection could go undetectable and its potential transmission to humans.

The Study on Signal to Noise Ratio of Single-Shot Turbo Spin Echo to Reduce Image Distortion in Brain Stem Diffusion MRI (뇌줄기 확산강조 자기공명영상검사 시 뒤틀림을 줄이기 위한 SS-TSE 기법의 신호대잡음비 연구)

  • Koo, Nohyun;Lee, Hobeom;Choi, Kwanwoo;Son, Soonyong;Yoo, Beonggyu
    • Journal of the Korean Society of Radiology
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    • v.10 no.4
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    • pp.241-246
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    • 2016
  • The purpose of this study was to investigate the problems of low signal-to-noise ratio(SNR) of single-shot turbo spin echo(SS-TSE) by quantifying numerically decreased signal to noise ratio. Thirty five patients without brain disease underwent diffusion MRI in 3T scanner from July to October in 2015. Single shot echo planar imaging(SS-EPI) which is conventionally used in MRI was taken to compared SS-TSE in SNR of medulla oblongata. As a result, SNR of SS-TSE diffusion(b0=$314.41{\pm}42.96$, b1000=$117.33{\pm}14.04$) is than SS-EPI diffusion(b0=$514.84{\pm}48.97$, b=$208.65{\pm}25.70$) lower in b=0 image(38.9%) and b=1,000 image(43.8%). Thus, diffusion MR using SS-EPI of MS-EPI should be taken for diagnosis of disease in brain stem due to decreased SNR of diffusion using SS-TSE.

The Effects of Shinbi-tang and Gamishinbi-tang on Immune Cell and Serum OA-specific IgE in BALF in Rat Asthma Model (신비탕 및 가미신비탕이 Allergy 천식 모델 흰쥐의 BALF내 면역세포 및 혈청 IgE에 미치는 영향에 관한 연구)

  • 김승수;정희재;정승기;이형구
    • The Journal of Korean Medicine
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    • v.23 no.2
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    • pp.198-210
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    • 2002
  • Background : Asthma is a chronic inflammatory disorder under immunological influence. Shinbi-tang and Gamishinbi-tang are herbal decoctions used for treating asthma in traditional herbal medicine. Objective : To evaluate the effects of Shinbi-tang and Gamishinbi-tang on immune cell & serum OA-specific IgE in broncho-alveolar lavage fluid (BALF) in rat asthma model. Material and Methods : Rats were sensitized with ovalbumin (OA); at day 1 sensitized group and Shinbi-tang and Gamishinbi-tang groups were systemically immunized by subcutaneous injection of 1mg OA and 300mg of Al(OH)$_3$ in a total volume of 2ml. At the same time, 1 ml of 0.9% saline containing 6 x 10$^{9}$ B. pertussis bacilli was injected by i.p. 14 days after the systemic immunization, rats received local immunization by inhaling 0.9% saline aerosol containing 2%(wt/vol) OA. A day after local immunization, HAL fluid was collected from the rats. Rats were orally administered with each of Shinbi-tang and Gamishinbi-tang extract for 14 days from the day after local immunization. Lymphocyte, CD4+ T cell CD8+ T cell counts, CD4+/CD8+ ratio in BALF, change of serum OA-specific IgE level, CD4+ T cell CD8+ T cell percentages in the peripheral blood were measured and evaluated. Results : Shinbi-tang and Gamishinbi-tang showed an alleviating effect on asthmatic responses of rats. Shinbi-tang decreased total cell, lymphocyte, CD4+ T cell in BALF, serum OA-specific IgE level as compared with the control group. Gamishinbi-tang decreased total cell, lymphocyte, CD4+ T cell, and CD4+/CD8+ ratio in HALF as compared with the control group. CD4+/CD8+ ratio in HALF from Shinbi-tang group and serum OA-specific IgE level from Gamishinbi-tang group didn't show any significant variation from control group. CD8+ T cell in HALF, CD3+CD4+ T cell and CD3+CD8+ T cell percentages in peripheral blood showed no significant variation among groups. Conclusion : Shinbi-tang and Gamishinbi-tang alleviated asthmatic hypen-eactivity of the rat immune system through CD4+ T cell and serum IgE. Further the study of immune system modulating mechanism is expected.

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Gonadotropin Bioactivity and Steroids in Ovarian Follicle Matured by Hyperstimulation (과배란유도에 의해 성숙된 여포의 GTH 활성 도 와 스테로이드합성)

  • Yoon, Yong-Dal;Chun, Eun-Hyun;Kim, Moon-Kyoo;Kwon, Hyuk-Bang
    • Clinical and Experimental Reproductive Medicine
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    • v.16 no.2
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    • pp.119-130
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    • 1989
  • 본 연구는 생식주기중 폐쇄여포액내에서 생물학적, 면역학적 특성을 나타내는 GTH 의 변화를 조사하고 steroid hormone과의 상관관계를 조사하며 국부조절인자로서 의 GTH의 역 활을 조사하고자 하였다. 가임기간중 215개의 여포와 IVF과정에서 185개의 여포를 얻어 여포액내 GTH의 생물학적 또는 면역학적 활성을 측정하였다. Bioactive LH(bLH)는 생쥐의 Leydig cell-testosterone production assay, bFSH는 흰쥐의 Sertoli cell aromatase assay로 측정 하였 다. Immunological GTH(iLH , iFSH) 는 MaiaClone RIA , Delfia kits를 사용하였다. 여포액내 iLH, iFSH , ihCG 는 hyperstimulation에 의해 형성된 여포의 크기와는 무관하였다. 또 hMG, huFSH 의 처리와도 상관성이 없었다. T의 농도가 높은 여포액내의 iFSH는 현저히 낮았으며 E, P 가 고농도인 여포의 ihCG 양은 현저히 낮았다. 과배란이 유도된 난소의 여포액내 iLH는 LH specific RIA로 측정시 3mIU/ml 이하이었다. 생식주기중 여포액내 bLH, bFSH는 배란기에 현저히 증가 하였다. 혈청내 GTH B/I ratio는 엘정한 반면 여포액내 LH,FSH의 생물학적, 면역학적 활성은 미수정란을 가지거나 폐쇄된 여포내의 활성보다 현저하게 높았다. 위의 결과로 보아 여포액내 생식소자극호르온은 면역학적활성보다 높은 생물학적 활성을 가지며, 생리적 현상의 지표가 된다고 추론된다. 또한 steroid, bGTH는 여포의 선택, 폐쇄를 구분하는 지표로 사용가능하며, 여포가 폐쇄될때 여포액내 B/I ratio가 현저히 낮아지는 것으로 보아 GTH의 활성이 감소되는 것으로 판단된다.

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Magnetic Propertes of $Nd_{x}{(Fe_{0.9}Co_{0.1})}_{90-x}B_{6}Nb_{3}Cu_{1}(x=\;3,\;4,\;5)$ Nanocrystalline Alloys ($Nd_{x}{(Fe_{0.9}Co_{0.1})}_{90-x}B_{6}Nb_{3}Cu_{1}(x=\;3,\;4,\;5)$ 초미세결정립합금의 자기특성)

  • 조용수;김만중;천정남;김택기;박우식;김윤배
    • Journal of the Korean Magnetics Society
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    • v.5 no.5
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    • pp.880-894
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    • 1995
  • Magnetic properties of $Nd_{x}{(Fe_{0.9}Co_{0.1})}_{90-x}B_{6}Nb_{3}Cu_{1}(x=\;3,\;4,\;5)$ rrelt-spun alloys with 6 at% B content were studied aiming for finding out a new $\alpha$-Fe based Nd-Fe-B nanocrystalline alloy with good hard magnetic properties. $Nd_{x}{(Fe_{0.9}Co_{0.1})}_{90-x}B_{6}Nb_{3}Cu_{1}$ melt-spun alloys prepared by RSP crystallized to nanocrystalline phase. An optimally annealed $Nd_{3}{(Fe_{0.9}Co_{0.1})}_{87}B_{6}Nb_{3}Cu_{1}$ melt-spun alloys had larger volume ratio of $\alpha$-Fe(Co) than that of higher Nd content alloy and showed high remanence of about 1.6 T. On the contrary, the increase of Nd content in $Nd_{x}{(Fe_{0.9}Co_{0.1})}_{90-x}B_{6}Nb_{3}Cu_{1}$ alloys gave rise to gradual increase of an amount of $Nd_{2}{(Fe,\;Co)}_{14}B$ phase and improved coercivity. An optimally annealed $Nd_{5}{(Fe_{0.9}Co_{0.1})}_{85}B_{6}Nb_{3}Cu_{1}$ alloy showed the most improved hard mag¬netic properties. The remanence, coercivityand energy product of the alloy were 1.35 T, 219 kA/m (2.75 kOe), and $129\;kJ/m^{3}$ (16.2 MGOe), respectively.

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