• Title/Summary/Keyword: System T/H Analysis

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Homologous Expression and Quantitative Analysis of T3SS-Dependent Secretion of TAP-Tagged XoAvrBs2 in Xanthomonas oryzae pv. oryzae Induced by Rice Leaf Extract

  • Kim, S.H.;Lee, S.E.;Hong, M.K.;Song, N.H.;Yoon, B.;Viet, P.T.;Ahn, Y.J.;Lee, B.M.;Jung, J.W.;Kim, K.P.;Han, Y.S.;Kim, J.G.;Kang, L.W.
    • Journal of Microbiology and Biotechnology
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    • v.21 no.7
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    • pp.679-685
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    • 2011
  • Xanthomonas oryzae pv. oryzae (Xoo) produces a putative effector, XoAvrBs2. We expressed XoAvrBs2 homologously in Xoo with a TAP-tag at the C-terminus to enable quantitative analysis of protein expression and secretion. Addition of rice leaf extracts from both Xoo-sensitive and Xoo-resistant rice cultivars to the Xoo cells induced expression of the XoAvrBs2 gene at the transcriptional and translational levels, and also stimulated a remarkable amount of XoAvrBs2 secretion into the medium. In a T3SS-defective Xoo mutant strain, secretion of the TAPtagged XoAvrBs2 was blocked. Thus, we elucidated the transcriptional and translational expressions of the XoAvrBs2 gene in Xoo was induced in vitro by the interaction with rice and the induced secretion of XoAvrBs2 was T3SSdependent. It is the first report to measure the homologous expression and secretion of XoAvrBs2 in vitro by rice leaf extract. Our system for the quantitative analysis of effector protein expression and secretion could be generally used for the study of host-pathogen interactions.

Exergy-Based Performance Analysis of Heavy-duty Gas Turbine in Part-Load Operating Conditions (엑서지를 이용한 대형 발전용 가스터빈의 부분부하 성능 분석)

  • Song, T.W.;Sohn, J.L.;Kim, J.H.;Kim, T.S.;Ro, S.T.
    • Proceedings of the KSME Conference
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    • 2001.06d
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    • pp.751-758
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    • 2001
  • Exergy concept is applied to the analysis of part-load performance of gas turbine engine. Exergy is a useful tool to find the source of irreversibility in thermal system. In this study, details of the performance characteristics of a heavy-duty gas turbine, l50MW-class GE 7FA model, are described by theoretical investigations with exergy analysis. Result shows that exergy destruction rate of gas turbine increases with decreased load, which means increase of irreversibility. Also, it is found that variations of IGV angle and amount of cooling air for turbine blades are closely related to the inefficiencies of compressor and turbine, respectively.

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A Study on the Water Quality Characteristics of the Reservoirs in Gwangju City (광주지역 저수지수 수질특성에 관한 연구)

  • Kim, Dong-Soo;Park, Jong-Whan;Kim, Yun-Hee;Song, Hyung-Myung;Park, Jong-Tae
    • Journal of Environmental Science International
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    • v.18 no.12
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    • pp.1383-1390
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    • 2009
  • This study was performed to investigate the physico-chemical properties and phytoplankton concentration from February to December, 2007 in Gwangju area reservoirs. Water samples from 20 reservoirs were analyze d. As results of the water quality analysis, the average pH was 7.6 and annual pH were 6.3~9.6. The higher pH of 8.6~9.6 were showed from March to August due to eutrophication. Chlorophyll-a concentration ranged from 0.4 to $164.3\;mg/m^3$. The highest chlorophyll-a concentration was observed in August. BOD was correlated with SS, T-N, and Chlorophyll-a(R=0.82, 0.90 and 0.84) respectively. COD was correlated with BOD, SS and Chlorophyll-a(R= 0.89, 0.77 and 0.76) respectively. The T-N/T-P ratios were 4~281, so phosph orus was considered to be the limiting factor in most of points. The trophic state showed eutrophicate states in Gwangju reservoirs. Therefore it was necessary to monitor continuously. In order to monitor the reservoirs, an algae prediction system must be used.

Numerical investigation and optimization of the solar chimney performances for natural ventilation using RSM

  • Mohamed Walid Azizi;Moumtez Bensouici;Fatima Zohra Bensouici
    • Structural Engineering and Mechanics
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    • v.88 no.6
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    • pp.521-533
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    • 2023
  • In the present study, the finite volume method is applied for the thermal performance prediction of the natural ventilation system using vertical solar chimney whereas, design parameters are optimized through the response surface methodology (RSM). The computational simulations are performed for various parameters of the solar chimney such as absorber temperature (40≤Tabs≤70℃), inlet temperature (20≤T0≤30℃), inlet height of (0.1≤h≤0.2 m) and chimney width (0.1≤d≤0.2 m). Analysis of variance (ANOVA) was carried out to identify the design parameters that influence the average Nusselt number (Nu) and mass flow rate (ṁ). Then, quadratic polynomial regression models were developed to predict of all the response parameters. Consequently, numerical and graphical optimizations were performed to achieve multi-objective optimization for the desired criteria. According to the desirability function approach, it can be seen that the optimum objective functions are Nu=25.67 and ṁ=24.68 kg/h·m, corresponding to design parameters h=0.18 m, d=0.2 m, Tabs=46.81℃ and T0=20℃. The optimal ventilation flow rate is enhanced by about 96.65% compared to the minimum ventilation rate, while solar energy consumption is reduced by 49.54% compared to the maximum ventilation rate.

The Aerodynamic Analysis of Pantograph of the Next Generation High Speed Train (차세대 고속철도 판토그래프의 공력특성 해석)

  • Kang, H.M.;Kim, C.W.;Cho, T.H.;Yoon, S.H.;Kwon, H.B.;Park, C.S.
    • 한국전산유체공학회:학술대회논문집
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    • 2011.05a
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    • pp.362-367
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    • 2011
  • The aerodynamic performance of the pantograph of the next generation high sped train is analyzed. The calculation of the flow around pantograph is carried cut by FLUENT; by the steady state flow calculation with ${\kappa}-{\omega}$ SST turbulence model, the lift force of the pantograph is computed. For the verification of the numerical schemes am grid systems, flow calculations are performed with the pantograph shape which was used at the experiments performed at Railway Technical Research Institute (RTRI) in Japan. Then, the difference of lift force between numerical am experimental results is about 10%. Therefore, selected numerical schemes and the current grid system is adequate for the analysis am prediction of the aerodynamic performance of panthograph system. Based on these numerical schemes am grid system, the flow around pantograph of the next generation high sped train is calculated and the lift force of the pantograph is predicted; the lift force of the pantograph is about 146N.

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COMPUTATIONAL FLOW ANALYSIS ACCORDING TO ORIFICE POSITION IN FEEDING LINE OF LRE (액체로켓엔진 공급배관 내 오리피스 위치에 따른 유동해석)

  • Kim, H.M.;Kim, W.J.;Roh, T.S.
    • 한국전산유체공학회:학술대회논문집
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    • 2010.05a
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    • pp.451-455
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    • 2010
  • In this study, the various supply system of LRE such as a feed-line, an elbow, and an orifice as a part of integrated analyzing has been combined to develop the performance analysis program. Computational analysis has been used to compare the results and to verify the validity and limitation conditions of the performance analysis program by changing orifice positions.

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Loading Path Optimization in Aluminum Tube Hydroforming using Response Surface Method (반응표면법을 이용한 알루미늄 튜브 하이드로포밍의 하중경로 최적화)

  • Lim, H.T.;Kim, H.J.;Kim, H.Y.
    • Proceedings of the Korean Society for Technology of Plasticity Conference
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    • 2007.05a
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    • pp.314-317
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    • 2007
  • Automotive rear subframe of aluminum tube was developed by using hydroforming process, based on the numerical analysis and physical tryouts. In the previous study, the effect of prebending was evaluated on the basis of forming limit diagram which had been obtained from free bulging, T-shape forming and cross-shape forming, using the developed tube hydroformability testing system. In order to get the sound products, appropriate internal pressure is to be imposed corresponding to the axial feeding. In this study, the loading path, the combination of internal pressure and axial feeding during the process, was optimized to ensure minimum thickness variation and dimensional accuracy, by using response surface method.

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Sensitivity Analysis of Probabilistic Reliability Evaluation of KEPCO System Using TRELSS (TRELSS를 이용한 한전계통의 확률론적 신뢰도 평가의 감도해석)

  • Tran, T.T.;Kwon, J.J.;Choi, J.S.;Jeon, D.H.;Park, Y.S.;Han, G.N.
    • Proceedings of the KIEE Conference
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    • 2005.11b
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    • pp.234-236
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    • 2005
  • The importance and necessity conducting studios on grid reliability evaluation have been increasingly important In recent years due to the number of black-out events occurring through in the world. Quantity evaluation of transmission system reliability is very important in a competitive electricity environment. The reason is that the successful operation of electric power under a deregulated electricity market depends on transmission system reliability management. Also in Korea it takes places. The results of many case studios fer the KEPCO system using the Transmission Reliability Evaluation for Large-Scale Systems (TRELSS) Version 6_2, a program developed by EPRI are introduced in this paper. Some sensitivity analysis has been Included in case study. This paper suggests that the some Important input parameters of the TRELSS can be determined optimally from this sensitivity analysis fer high reliability level operation of a system.

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Analysis of Ventilation Efficiency by Duct System in Pig House (돈사 덕트 환기시스템의 효율 분석)

  • Song, J.I.;Yoo, Y.H.;Lee, D.S.;Choi, H.C.;Kang, H.S.;Kim, T.I.;Jeon, B.S.;Park, C.H.;Kim, H.H.
    • Journal of Animal Environmental Science
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    • v.8 no.2
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    • pp.73-78
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    • 2002
  • The experiment was carried out to investigate the optimal air velocity for improving the ventilation efficiency of duct ventilation system used in Korean swine building. The results are followed ; In 2.2 m height of duct, the air velocity of hole was 5.0 m/s as the over level of recommendation. In different hole interval, the air velocity was various of 4.6${\sim}$11.6 m/s in narrow hole interval, 5.4${\sim}$10.9 m/s in broad hole interval. But the air velocity was 6.6${\sim}$7.7 m/s in duct system pierced hole with equal interval, and it was equal velocity in different parts of duct in this hole interval.

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