• Korean Journal of Veterinary Research
• /
• v.39 no.1
• /
• pp.45-54
• /
• 1999

In the present study we have analyzed the characteristics and distribution of the mu-opioid receptor(MOR) by raising anti-peptide antisera to the C-terminal peptide of MOR. The antisera against MOR was produced in New Zealand White rabbit against 15 residue corresponding to amino acids, 384-398 of the cloned rat MOR. The antigenic peptide was synthesized using an Applied Biosystems 432 solid-phase peptide synthesizer. The specificity and identification of the antisera were tested by analysis of transfected cells, epitope mapping and immunohistochemical method. COS-7 cells electroporated with MOR cDNA were used to evaluate the characteristics and subcellular distribution of MOR. MOR immunoreactivity was prodominent in the plasmalemma and subcellular compartments such as endoplasmic reticulum, Golgi apparatus and vesicle like structure. Furthermore, both tissue sections and transfected cell lines could be immunostained with these antisera and the immunoreactivity was abolished when anti-MOR sera were preincubated with the peptide against which they were raised. Based on epitope mapping analysis, all antisera appeared to have a similar epitope, which was determined to be within the last amino acid, 391-398. Moreover, immunohistochemistry showed that MOR immunoreactivity was observed in many brain areas including cerebral cortex, striatum, hippocampus, locus coeruleus and the superficial laminae of the dorsal horn. These stained spinal cord and brain areas showed the mirrored pattern observed in auto radiographic studies of mu-opioid binding as well as a pattern similar to that seen by is situ hybridization for MOR. Thus, several lines of evidence support the conclusion that the antisera produced in the present study most likely recognize mu-opioid receptor. These results suggest that MOR antisera may be utilized as useful tool to analyze the physiological and pharmacological studies for mu-opioid receptor in the future.

• Korean Journal of Veterinary Research
• /
• v.39 no.3
• /
• pp.583-592
• /
• 1999

We have developed the in situ hybridization(ISH) technique for rapid diagnosis of canine distemper(CD) which is the major infectious disease in dogs. In our experiment, we rapidly detected distribution of the specific canine distemper viral genome without disrupting morphology of tissues or cells. Two oligonucleotide probes for ISH were synthesized chemically and labelled 5' end with nonisotopic biotin by DNA synthesizer. The whole procedures of ISH was completed within 1~2 hours using the Microcapillary action system. On histological study, typical cytoplasmic or intranuclear inclusion bodies were observed in the trachea, bronchiole, brain, and urinary bladder with the presence of prominent red positive signals on ISH, indicating specific CDV genome from the paraffin-embedded tissues of infected 13 cases. The results showed ISH can be used as a rapid and effective diagnostic method for diagnosis of CD.

• Journal of the Korean Institute of Intelligent Systems
• /
• v.17 no.7
• /
• pp.957-963
• /
• 2007

In this paper, Nonlinear Autoregressive (NAR) method based on Least Square-Support Vector Regression (LS-SVR) is introduced and tested for nonlinear sustained vowel modeling. In the database of total 43 sustained vowel of Benign Vocal Fold Lesions having aperiodic waveform, this nonlinear synthesizer near perfectly reproduced chaotic sustained vowels, and also conserved the naturalness of sound such as jitter, compared to Linear Predictive Coding does not keep these naturalness. However, the results of some phonation are quite different from the original sounds. These results are assumed that single-band model can not afford to control and decompose the high frequency components. Therefore multi-band model with wavelet filterbank is adopted for substituting single band model. As a results, multi-band model results in improved stability. Finally, nonlinear sustained vowel modeling using NAR based on LS-SVR can successfully reconstruct synthesized sounds nearly similar to original voiced sounds.

• The Journal of Korean Institute of Electromagnetic Engineering and Science
• /
• v.19 no.10
• /
• pp.1104-1114
• /
• 2008

In the output of a mixer, spurious appears with the desired signal, and a filter is necessary to suppress the spurious. In this paper, the planar filter for Ka-band frequency synthesizer was designed and fabricated. In this procedure, the frequency response becomes asymmetric because of discontinuities at the high frequency. Using this, we designed short-end PCLF by using a individual resonator tuning method. The fabricated 5th-order Ka-band pass filter is compared with the result of EM simulation through measurement. The performance agrees with the simulation. Finally spurious suppression was examined through the measurement of output spectrum of the mixer with the filter.

• The Journal of Korean Institute of Communications and Information Sciences
• /
• v.23 no.8
• /
• pp.2064-2071
• /
• 1998

A new digital clock level translator has been designed in order to produce a clock source of the internal logic circuits. The translator output has 50% duty ratio from small sinusoidal input such as TCXO which oscillates itself in poratable components. The circuit consists of positive and negative comparators, RS latch, charge pump, and reference vol- tage generator. It detects pulse width of the output waveform and feedbacks the control signal to the input com-parator. It detects pulse width of the output waveform and feedbacks the control signal to the input com-parator reference, producing output waveform with valid 50% duty ratio of the digital signal level. The designed level translator can be used as a sampling clock source of ADC, PLL and the colck source of the clock synthesizer. The circuit wasdesigned in a 0.8.mu.m analog CMOS technology with double metal, double poly, and BSIM3 circuit simulation model. From our experimental results, a stable operating characteristics of 50 +3% duty ratio was obtained from the sinusoidal input wave of 370 mV.

• The Journal of Korean Institute of Electromagnetic Engineering and Science
• /
• v.28 no.8
• /
• pp.610-618
• /
• 2017

This paper presents a 868/915 MHz CMOS RF transceiver for the ZigBee application. Using a switchless matching network, the off chip switch is removed to achieve the low cost RF transceiver, and by the elimination of the switch's insertion loss we can achieve the benefits for the RF receiver's noise figure and transmitter's power efficiency at the given output power. The receiver is composed of low-noise amplifier, mixer, and baseband analog(BBA) circuit. The transmitter is composed of BBA, mixer, and driver amplifier. And, the integer N type frequency synthesizer is designed. The proposed ZigBee RF full transceiver is implemented on the $0.18{\mu}m$ CMOS technology. Measurement results show that the maximum gain and the noise figure of the receiver are 97.6 dB and 6.8 dB, respectively. The receiver consumes 32 mA in the receiver mode and the transmitter 33 mA in the transmission mode.

• Proceedings of the Korean Institute of Information and Commucation Sciences Conference
• /
• 1999.11a
• /
• pp.161-166
• /
• 1999

The global trends of mobile communication system is moving toward digitizing, high-speed and large-capacity. Also, to utilize the limited frequency-resource efficiently, spread spectrum system is a mainstream. In this study we are concerning with the fast frequency-hopping system. Instead of the PLL with many problems such as phase-noise, we used the DDS is popular in these days minimizes the disadvantage of PLL. In the case the FFH system is designed using the PLL, it is difficult to be satisfied of the design conditions such as RF badwidth and the settling time of PLL, and it has limitation because of complex circuit by using the balanced modulator. In this study, we evaluated the performance in order to design the FFH system using the DDS. The system that has the improvement of error rate, 1Mhps hopping rate and 5MHz RF bandwidth is designed and evaluated.

• The Transactions of the Korean Institute of Power Electronics
• /
• v.10 no.2
• /
• pp.109-116
• /
• 2005

The high performance drives of the slotless Permanent Magnet Brushless DC(PM BLDC) motor can be achieved by the current control, where the currents flow according to the rotor position and the current phase is suitably controlled according to the operational condition. Rotor position information can be provided by Hall-IC or sensorless algorithm. So, the Hall-ICs are set up in this motor to detect the main flux from the rotor. Instead of using three Hall-ICs and encoder, this paper uses only two Hall-ICs for the permanent magnet rotor position and the speed feedback signals, and uses a micro-controller of 16-bit type (80C196KC). Also because of low resolution obtained by using Hall-IC even low-cost and simple structure, to improve the wide range of speed response characteristic more exactly, we propose the rotor position signal synthesizer using PLL circuit based on two Hall-ICs.

• Journal of the Korea Institute of Information and Communication Engineering
• /
• v.5 no.4
• /
• pp.668-675
• /
• 2001

Recently, the performance of the commercial PCS(Personal Communication Service) system has been improved to the uppermost limit and ultimately the next generation mobile communication is to be realized by IMT-2000 (International Mobile Communication-2000) to provide multimedia services. Therefore, the new type receiving system is researched actively and one of the most important part in a receiver is direct conversion method. The direct conversion method is suitable for low power consumption, small size, MMIC, and low price, which is to be adopted to the next generation mobile communication systems. In this case, however, several problems occur due to DC-offset. The DC-offset suppresses amplification of the required signal because of the leakage signal of frequency synthesizer in the system. In this thesis, the removing method of DC-offset was considered. There are four removing techniques of DC-offset, which are AC-coupling, large capacitor, DC-feedback loop, and DC-free coding. Among these, the AC-coupling method is the most simplest method and the DC-feedback loop method has the best performance. Then, the performance of the AC-coupling method and DC-feedback loop method are evaluated by HP's ADS simulation tool. As a result, the AC-coupling method cannot be used to the digital communication systems due to data loss. On the other hand, it was confirmed that the DC-feedback loop method is suitable for the direct conversion receiver.

• Journal of Radiation Industry
• /
• v.11 no.1
• /
• pp.13-18
• /
• 2017

Recently, the number of PET cyclotrons has increased in Korea. A cyclotron mainly produces $^{18}F$, which is used for the production of [$^{18}F$]FDG, a cancer diagnostic radiopharmaceutical. For radiation protection, the discharge control standard under the Nuclear Safety Act limits the radioactive concentration of $^{18}F$ in the exhaust discharged from a nuclear power utilization facility to below $2,000Bq\;m^{-3}$. However, the radioactive concentration of $^{18}F$ discharged during [$^{18}F$]FDG production at the cyclotron facility at Chosun University is maintained at about $1,500Bq\;m^{-3}$ on average, which is 75% of the concentration limit of the discharge control standard, and temporarily exceeds the standard as per the real-time monitoring results. This study evaluated the performance of the exhaust filter unit of the cyclotron facility at Chosun University by assessing the concentration of $^{18}F$ in the exhaust, and an experiment was conducted on the discharge reduction, where $^{18}F$ is discharged without reacting with the FDG precursors during [$^{18}F$]FDG synthesis and is immediately captured by the [$^{18}F$]FDG automatic synthesis unit. Based on the performance evaluation results of the exhaust filter at the cyclotron facility of Chosun University, the measured capture efficiency before and after the filter was found to be 92%. Furthermore, the results of the discharge reduction experiment, where the exhaust $^{18}F$ was immediately captured by the [$^{18}F$]FDG synthesizer, showed a very satisfactory 94.3% reduction in the concentration of discharge compared to the existing discharge concentration.