• Mycobiology
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• 제51권2호
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• pp.94-108
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• 2023

Termitomyces sp. that grow in symbiosis with fungus-farming Termites have medicinal properties. However, they are rare in nature, and their artificial culture is challenging. The expression of AXL receptor tyrosine kinase and immune checkpoint molecules favor the growth of cancer cells. The study evaluated the optimal conditions for the artificial culture of Termitomyces and their inhibitory activity on AXL and immune checkpoint molecules in lung adenocarcinoma and melanoma cell lines. The culture of 45 strains of Termitomyces was compared. Five strains with marked growth rates were selected. Four of the selected strains form a single cluster by sequence analysis. The mycelium of 4 selected strains produces more fungal mass in potato dextrose broth than in a mixed media. The bark was the most appropriate solid substrate for Termitomyces mycelia culture. The mycelium of all five selected strains showed a higher growth rate under normal CO₂ conditions. The culture broth, methanol, and ethyl acetate of one selected strain (T-120) inhibited the mRNA relative expression of AXL receptor tyrosine kinase and immune checkpoint molecules in cancer cell lines. Overall, these results suggest the potential usefulness of Termitomyces extracts as a coadjuvant therapy in malignant diseases.

• 한국치위생학회지
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• 제22권6호
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• pp.563-572
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• 2022

Objectives: The purpose of this study is to provide basic data for elucidating the clinical experiences of nursing assistants working at dental clinics and for establishing the bases necessary for building a safe working environment for the staff at dental clinics. Methods: From March 28 to August 20, 2022, the study conducted in-depth interviews with nursing assistants working with dental hygienists in dental clinics. The collected data were analyzed using the phenomenological method. Results: The study analyzed the meanings associated with the dental clinical assistance work experiences of nursing assistants in dental clinics. A total of 98 semantic words and 9 sub-categories were extracted, which were ultimately categorized into 4 central meanings: 'imperfect reality', 'work without boundaries', 'putting oneself in someone else's shoes', and 'symbiosis'. Conclusions: The nursing assistants were exposed to an environment where they needed to perform delegated treatment procedures even when they lacked dental work competency. They recognized their gaps in learning and the necessity for designated roles as clinical assistants. Thus, it is necessary to review the allocation and expansion of the work roles of dental practitioners based on the practical and rational realities in dental practice.

• Journal of Microbiology and Biotechnology
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• 제33권6호
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• pp.745-752
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• 2023

Gut symbionts play crucial roles in host development by producing nutrients and defending against pathogens. Phloem-feeding insects in particular lack essential nutrients in their diets, and thus, gut symbionts are required for their development. Gram-negative Pantoea spp. are known to be symbiotic to the western flower thrips (Frankliniella occidentalis). However, their bacterial characteristics have not been thoroughly investigated. In this study, we isolated three different bacteria (BFoK1, BFiK1, and BTtK1) from F. occidentalis, F. intonsa, and T. tabaci. The bacterial isolates of all three species contained Pantoea spp. Their 16S rRNA sequences indicated that BFoK1 and BTtK1 were similar to P. agglomerans, while BFiK1 was similar to P. dispersa. These predictions were supported by the biochemical characteristics assessed by fatty acid composition and organic carbon utilization. In the bacterial morphological analysis, BFoK1 and BTtK1 were distinct from BFiK1. All these bacteria were relatively resistant to tetracycline compared to ampicillin and kanamycin, in which BFoK1 and BTtK1 were different from BFiK1. Feeding ampicillin (100,000 ppm) reduced the bacterial density in thrips and retarded the development of F. occidentalis. The addition of BFoK1 bacteria, however, rescued the retarded development. These findings indicate that Pantoea bacteria are symbionts to different species of thrips.

• 대순사상논총
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• 제18권
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• pp.25-52
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• 2004

The history of the mankind, that is the history of the war, have been continued with the consecutive of the struggle and confrontation. In the history of many war, the most important factor of them is just the religion. so that today, for the mankind to long for the peace, all believers are charged with very important mission. Confronting with the 21th century, yet the war is not ceasing in an earth one side. At this time if we are to investigate the problem of real peace, we need to present uprightly the direction of basic religious factor and solution. In Korea, which is keeping deep religious mind, the Daesoon thought that appears newly at the modern times is proposing the 'Haewonsangsaeng'(eliminating resentment and helping one another) as new peace idea of 21th century. In this thought, we can discover the reason of the conflict to appear in the history of mankind. And there is a new idea of peace, that is to say, which is called 'sangsaeng'(mutual aid and cooperation). In this article, centering upon Sangsaeng idea, I try to introduce the new viewpoint of Daesoon thought about the conflict and peace.

• 한국수학교육학회지시리즈A:수학교육
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• 제63권2호
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• pp.233-254
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• 2024

This paper delves into the symbiotic integration of coding and mathematics education, aimed at cultivating computational thinking and enriching mathematical problem-solving proficiencies. We have identified a corpus of scholarly articles (n=38) disseminated within the preceding two decades, subsequently culling a portion thereof, ultimately engendering a contemplative analysis of the extant remnants. In a swiftly evolving society driven by the Fourth Industrial Revolution and the ascendancy of Artificial Intelligence (AI), understanding the synergy between these domains has become paramount. Mathematics education stands at the crossroads of this transformation, witnessing a profound influence of AI. This paper explores the evolving landscape of mathematical cognition propelled by AI, accentuating how AI empowers advanced analytical and problem-solving capabilities, particularly in the realm of big data-driven scenarios. Given this shifting paradigm, it becomes imperative to investigate and assess AI's impact on mathematics education, a pivotal endeavor in forging an education system aligned with the future. The symbiosis of AI and human cognition doesn't merely amplify AI-centric thinking but also fosters personalized cognitive processes by facilitating interaction with AI and encouraging critical contemplation of AI's algorithmic underpinnings. This necessitates a broader conception of educational tools, encompassing AI as a catalyst for mathematical cognition, transcending conventional linguistic and symbolic instruments.

• Animal cells and systems
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• 제6권3호
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• pp.227-232
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• 2002

GroESLx proteins of symbiotic X-bacteria were overproduced in Escherichia coli and their structural characteristics were assayed after simple purification. The GroESx and GroELx were heat-stable at 8$0^{\circ}C$ and 5$0^{\circ}C$, respectively. After heat-treatment, GroESx was purified by DEAE Sephadex A-50 chromatography and GroELx was purified by step- and linear sucrose density gradient ultracentrifugation. Molecular masses of GroESx and GroELx were 50-80 kDa and 800 kDa, respectively, as estimated by sucrose density gradient ultracentrifugation. In chemical cross-linking analysis, subunits of GroESx were mostly cross-linked by incubation for 3 h in 0.4% glutaralde-hyde and GroESx was found to be composed of homo-heptamer subunits. Those of GroELx were cross-linked within 10 min in 0.3% glutaraldehyde and GroELx was in two stacks of homo-heptamer subunits. On the other hand, GroESx and GroELx proteins in a solution could not be cross-linked even after incubation for 3 h in 0.5% glutaraldehyde. GroELx was stable at 4-37$^{\circ}C$. In the presence of both GroESx and ATP, GroELx$_{14}$ was stable at 37$^{\circ}C$ but not at 4$^{\circ}C$ or 24$^{\circ}C$. Thus, we confirmed the oligomeric properties of GroESx$_{7}$ and GroELx$_{14}$ and their stability to heat and in the interaction with GroESx.x.

• Journal of Microbiology and Biotechnology
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• 제18권3호
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• pp.490-496
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• 2008

Symbiobacterium toebii is a commensal symbiotic thermophile that cannot grow without support from a partner bacterium. We investigated the properties of Symbiobacterium growth-supporting factors (SGSFs) produced by the partner bacterium Geobacillus toebii. SGSFs occurred in both the cell-free extract (CFE) and culture supernatant of G. toebii and might comprise multifarious materials because of their different biological properties. The heavy SGSF contained in the cytosolic component exhibited heat- and proteinase-sensitive proteinaceous properties and had a molecular mass of >50 kDa. In contrast, the light SGSF contained in the extracellular component exhibited heat-stable, proteinase-resistant, nonprotein properties and had a molecular mass of <10 kDa. Under morphological examination using light microscopy, S. toebii cultured with the culture supernatant of G. toebii was filamentous, whereas S. toebii cultured with the CFE of G. toebii was rod-shaped. These results strongly suggest that the SGSFs produced by G. toebii comprise two or more types that differ in their growth-supporting mechanisms, although all support the growth of S. toebii. Upon the examination of the distribution of SGSFs in other bacteria, both cytosolic and extracellular components of Geobacillus kaustophilus, Escherichia coli, and Bacillus subtilis had detectable growth-supporting effects for S. toebii, indicating that common SGSF materials are widely present in various bacterial strains.

• 한국의류학회지
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• 제29권3_4호
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• pp.449-461
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• 2005

The purpose of this study is to rediscover the hidden value of non-mainstreamers style which has been overlooked for the past decade by investigate the aesthetics and formative features of the Grunge fashion. The Grunge fashion was derived from explosive popularity of the early 90's grunge music. Grunge was the alternative anti-thesis against mainstream pop music and anti-fashion against mainstream fashion. Dirty, rubbish grunge style of the poor street youth and grunge musicians have raised to the high fashion by designers. And These trial of designers made people to notice the value of the non-mainstreamers street style like grunge. Actually, the grunge brought the shock with many argument to the 90's fashion field. But now, It became the classic of the street fashion. And It has potent influence on the music, culture and high fashion. The Grunge is a kind of links between music and fashion, street fashion and high fashion, sub culture and mainstream culture, the past and now. Grunge isn't only a fashion of appearance. It is the attitude of wearing clothes and living a life. Variety grunge style in the international street fashion, high fashion, typical musicians's fashion of the 1990's and the 2000's was researched for this study. These materials were gathered from music magazines, fashion magazines, movies, musics and books. As a results of analysis, Grunge has the formative features like mix & match, layering, patchwork, primitive edge, rag, retro, recycle, kinderwhore, sneer scribbling, disheveled hair. Grunge also has the Aesthetic features like the beauty of $disorder{\cdot}\;disharmony{\cdot}\;incompleteness{\cdot}\;kitsch{\cdot}\;poverty{\cdot}\;alternative{\cdot}\;eclectic{\cdot}$symbiosis. For the last 10 years, These features changed our fashion be more pluralistic and dynamic.

• Animal cells and systems
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• 제15권4호
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• pp.315-324
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• 2011

The actiniarian sea anemone, Entacmaea quadricolor, and the scleractinian coral, Alveopora japonica, host symbiotic dinoflagellates belonging to the genus Symbiodinium (Freudenthal). We studied the host-symbiont specificity of these two anthozoan hosts in the northwestern Pacific Ocean. Symbionts within the two hosts were identified using partial large subunit (LSU) ribosomal DNA (rDNA) and complete internal transcribed spacers (ITS) 1 rDNA regions. The host, E. quadricolor, was identified using the partial LSU rDNA molecular marker. Genetic analysis showed that E. quadricolor only harbors dinoflagellates belonging to subclade C1/3 of the genus Symbiodinium. Moreover, no genetic variation was detected among the symbionts of E. quadricolor within the study region (Korea and Japan), even though the two distant sites were separated by more than 1000 km, at collection depths of 1 m in shallow and 13-16 m in deep water. Whilst scleractinian corals host multiple Symbiodinium clades in tropical waters, A. japonica, sampled over a wide geographical range (800 km) within the study region, only hosts Symbiodinium sp. clade F3. The high specificity of endosymbionts in E. quadricolor and A. japonica within the northwestern Pacific Ocean could be accounted for because symbiotic dinoflagellates within the host anemones appear to be acquired maternally, and the Kuroshio Current might affect the marine biota of the northwestern Pacific. However, the consistency of the symbiotic relationships between these two anthozoan hosts and their endosymbionts could change after climate change, so this symbiotic specificity should be monitored.

• 가족자원경영과 정책
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• 제16권1호
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• pp.21-39
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• 2012

The purpose of this study is to propose the direction of environmental education for early childhood children based on the environment-friendly life style and to improve the possibility of application of it at home and kindergarten. This study reviewed reflectively the early studies on the concepts of ecocentrism, the emerging process of it, the importance of environmental education for early childhood children based on the ecocentrism, and the practice of these kinds of education at home and kindergarten. The main concepts connected to the environment-friendly life style based on ecocentrism, that is, 'preservation of nature', 'interdepentence', 'wholeness', 'equilibrium' are the foundations of environmental education for early childhood children. When these kinds of concepts are considered, the aims of environmental education for early childhood children should be focused on the life style that attaches great importance to the symbiosis of human being and nature. The educational contents and methods in harmony with ecocentrism are as follows: The educational methods should be connected to the education based on ordinariness, the learning through adults' model, the family-community-centered activities, and ways of thinking of the unity of knowledge and conduct. And educational contents should include all of the life styles in clothes, food, and shelter. And the educational methods also should be connected to the education through joint working of children and teacher, play-centered education, activities considering ages and individual variations, and education connected home. And educational contents should include many different kinds of activities of experiencing nature outside home and kindergarten.