• Horticultural Science & Technology
• /
• v.29 no.1
• /
• pp.48-52
• /
• 2011

This study was conducted to establish the efficient screening system for resistant radish to Fusarium oxysporum f. sp. raphani. Five radish cultivars ('Myoungsan', 'Chungdu', 'Jangsaeng', 'Hannongyeorm', and 'Chungsukungjung') showing different degree of resistance to the fungus were selected. And the development of Fusarium wilt of the cultivars according to several conditions such as root wounding, dipping period of roots in spore suspension, inoculum concentration, and incubation temperature to develop the disease was tested. In distinguishing the resistance degree of the radish cultivars to the disease, non-cut roots were more effective than cut roots. And occurrence of Fusarium wilt of the radish plants increased in the proportion to increase of root-dipping period and spore concentration of the fungus. Thus, optimum conditions to differentiate susceptible and resistant cultivars to the disease were root-dipping period of 0.5 hour and spore concentration of $1{\times}10^7\;conidia{\cdot}mL^{-1}$. Disease severity of Fusarium wilt on the cultivars was changed with incubation temperature and the radish seedlings incubated at $25^{\circ}C$ represented the most difference of resistance and susceptibility to Fusarium wilt. From the above results, we suggest that the efficient screening method for resistant radish to Fusarium oxysporum f. sp. raphani would be to dip non-cut roots of fourteen-day-old radish seedlings in spore suspension of $1{\times}10^7\;conidia{\cdot}mL^{-1}$ for 0.5 hour and to transplant the inoculated plants to plastic pots with fertilized soil, and then to incubate the radish plants at a temperature of $25^{\circ}C$ for development of Fusarium wilt.

• 대한공업교육학회지
• /
• v.43 no.2
• /
• pp.1-23
• /
• 2018

This study analyzes the status of the NCS (National Competency Standards) based curriculum in which the apprenticeship program of vocational education and training high schools (VET high schools) participating in the apprenticeship system is included to present suggestions for organizing and evaluating the apprenticeship curriculum. For this, data on the status of curricula of 141 courses (75.8%) in 116 VET high schools participating in the apprenticeship system were analyzed. The results of the study are as follows. First, the average time assigned for the apprenticeship program was 1,054.2 hours and the apprenticeship program was organized in 12.4 courses in the school curriculum. The degree of evaluation per NCS competency unit of courses was found to be 95.4% for evaluation per competency unit and 5.6% for evaluation regardless of competency unit. Second, the average number of courses including apprenticeship programs in time assigned for the courses in school curriculum (when appropriate) was 11.5 (93.1%) and the average number of courses including apprenticeship programs in the contents of the school curriculum courses (when appropriate) was 10.6 (85.5%). Third, it was found that courses organized only for 'common competency units' were 8.5 (68.5%) and the number of courses in which 'student option competency units' were included was found to be 3.9 (31.5%). In addition to these study results, policy suggestions such as applying suspension on the approval period for change of curriculum by metropolitan and provincial offices of education, inspection on curriculum and enhanced consulting by metropolitan and provincial offices of education, providing plans to evaluate 'student option competency units', newly establishing 'subjects specialized for companies' or 'student option subjects' in national level curriculum are proposed.

• Asian Journal of Atmospheric Environment
• /
• v.7 no.2
• /
• pp.65-71
• /
• 2013

Japanese cedar (Cryptomeria japonica) pollinosis is the most popular pollinosis in Japan. It has been reported that Cryptomeria japonica pollen allergenic species are suspended as fine particles in the urban atmosphere. These allergenic fine particles are responsible for inducing asthma by breaking into the lower respiratory tract. It has also been found that pollinosis symptoms on the sufferers appear mainly at night-time by the results from epidemiological studies. However, the exact reason for these phenomena is not yet clarified. In this study, the diurnal and nocturnal behaviours of Cryptomeria japonica pollen grains and their allergenic species in the urban area of Saitama city of Kanto Plain were investigated. Airborne pollen grains and allergenic Cry j 1 concentrations in total suspended particulate matter (TSP) were investigated at two sampling sites, a heavy traffic road (roadside site) and at the balcony of the $10^{th}$ floor of the Building of Research and Project of Saitama University (general urban site). The latter sampling site where located about 300 m away from the roadside site was used as a general urban site unaffected by automobile traffic. The airborne pollen counts were measured with a real-time pollen monitor. Cry j 1 particles were collected with two high volume air samplers, and these concentrations were measured by surface plasmon resonance method with a Biacore J system. The diurnal variation of the airborne pollen counts was similar to the trends of temperature and wind speed during the day-time; whereas its tendency with wind speed trend was not observed during the night-time. Airborne pollen counts were lower with northern wind than with southern wind because the pollen comes from the mountainous areas, and the mountains in the south are closer, about half the distance to the northern mountains. It is suggested that the peaks of airborne pollen counts during night-time in the sampling site occurred by transport of pollen grains released during day-time in the mountainous forest areas, located c.a. 100 km away from the sampling site. On the roadside site the allergenic Cry j 1 concentrations were higher than at the general urban site, nevertheless pollen grains counts were lower. These results suggested that worsening of pollinosis symptoms during night-time in urban area was caused by transport of pollen grains during day-time in the mountainous forest areas. Moreover, pollen allergenic species become different morphology from pollen grain at roadside site, and the subsequent pollen grains re-suspension by automobile traffic.

• Proceedings of the Korean Society for Agricultural Machinery Conference
• /
• 2017.04a
• /
• pp.71-71
• /
• 2017

농용트랙터의 운전자는 작업, 주행으로 인한 유해한 저주파 진동에 장시간 노출된다. 이에 따라 운전자에게 전달되는 노면 진동을 감소시켜주기 위한 전방차축 현가장치의 역할이 커지고 있다. 트랙터의 전방차축 현가장치는 주로 유압식으로 설계되어 있으며 이를 구성하는 유압요소 선정이 현가장치의 성능에 중요한 영향을 미친다. 하지만, 실제와 유사한 조건에서 트랙터 차체 무게만큼 큰 부하를 제공하여 유압회로의 성능을 실험하는 것은 비용과 시간 측면에서 비효율적이다. 본 연구에서는 이를 대체하기 위하여 개별 유압요소의 성능을 테스트 할 수 있는 현가장치 유압회로 요인 시험기를 설계제작 하였다. 이를 이용하여 개별 부품의 성능곡선을 센서를 이용 측정하였고 얻은 특성값을, 구성한 유압 시뮬레이션 모델에 반영하여 실제조건의 유압특성을 얻을 수 있는 유효한 시뮬레이션 모델 개발에 활용하였다. 또한, 실험실 환경에서 유압식 현가장치를 간소화 시킨 형태로 유압회로의 성능을 예비시험해 볼 수 있도록 다양한 센서를 장착 데이터를 취득할 수 있도록 하였다. 개발한 요인 시험기는 하부에 설치된 가진 실린더를 이용하여 상부에 설치된 현가장치 실린더의 스트로크 변위와 속도에 따른 힘을 측정할 수 있도록 구성하였다. 이를 위해 현가장치 실린더의 헤드부와 로드부에 각각 압력센서를 설치하였으며 헤드부, 로드부의 압력 차이와 로드셀을 이용해 측정한 가진 실린더의 힘의 관계를 확인하였다. 상부의 현가 실린더 장치는 복동 형태로 제작되어 헤드부, 로드부 양쪽 방향으로 유량이 흐를 수 있도록 설계되었다. 이를 이용해 헤드부와 로드부 사이에 어큐뮬레이터, 가변 오리피스, 릴리프 밸브 등으로 유압회로를 구성하였으며 어큐뮬레이터 용량에 따른 힘의 변화, 가변 오리피스의 개도량에 따라서 전달되는 힘의 크기 등을 측정하였다. 하부의 가진 실린더는 사인파, 삼각파, 계단 입력, DC 레벨 등의 신호를 발생시킬 수 있도록 제작되었다. 신호의 주파수는 0~4Hz, 범위에서 사용자가 조절할 수 있도록 설정되었으며 계단응답 성능 측정 시험을 평가한 결과 정상상태오차는 0.470mm~0.536mm, 입상시간은 0.194초~0.202초, 정착시간은 0.230초~0.421초로 나타났다.

• Journal of the Korean GEO-environmental Society
• /
• v.21 no.8
• /
• pp.5-13
• /
• 2020

During the construction of underground space expansion of old facilities, it is necessary to secure temporary residence space for existing residents as well as noise and vibration issues during construction, and in the case of commercial, industrial, and social use, damage is expected from suspension of the use of facilities. There is a need for a technology that minimizes noise and vibration during underground expansion, enabling the use of existing facilities even during construction. In this study, a practical underground extension model is proposed by analyzing the behavior of the temporary structure and the surrounding ground as a result of measurement at each construction stage for a actual construction site. In order to solve the problems that occurred during construction, the basement slabs were placed in advance after the initial excavation. The measurement results (building inclinometer, crack measurement system, structure inclinometer and surface settlement meter) at the site were reviewed to analyze the behavior of the temporary structure and surrounding ground. As a result, it was confirmed that the inclinometer of the building and the structural inclinometer showed a tendency that the displacement after the slab line was placed was reduced or converged. The placement of basement slabs during underground extension not only relived the noise and vibration problems during construction, but also secured the stability of structures.

• Biomolecules & Therapeutics
• /
• v.6 no.1
• /
• pp.37-44
• /
• 1998

This study was performed to examine the effects of DA-9601, a novel antiulcer agent extracted from Artemisiae Herba, on radiation colitis in the rat. Female Wistar rats received a 30 Gy dose of irradiation to the 2 cm of distal colon in length using an intrarectal applicator system. 30 mg/tg or 100 mg/kg of DA- 9601 was administered orally 30 min before and 4 h after radiation on day 1. And the same dose of DA-9601 was given to the animals twice a day from day 2 to 14. As a reference control, sucralfate suspension (100 or 300 mg/head) was given as an enema based on the same treatment schedule of DA-9601. Body weight change and the frequency of diarrhea were recorded during the observation period as markers of radiationinduced injury, All animals were sacrificed on day 15 for evaluation of macro- and microscopic findings and mucosal myeloperoxidase (MPO) activity. Radiated animals showed diarrhea, mucosal redness and histologic changes characterized by edema and eosinophilic infiltration of the periglandular lamina propria with loss of colonic epithelium. Radiation also significantly increased mucosal MfO activity of affected colon f\\\\\\\\`<0.05). However, most of these changes were completely protected by oral administration with DA-9601. DA-9601 reduced radiation-induced histologic alteration significantly in a dose-related manner (P<0.05). In addition, mucosal MPO activity in rats receiving high dose of DA-9601 decreased significantly when compared with that in radiated control. High dose of sucralfate (300 mg/head) alleviated radiation-induced histologic lesion, but failed to reach statistical significance. The results of this study suggest that DA-9601 can be useful for the prevention of acute clinical symptoms of radiation proctocolitis and that decrease of mucosal MPO by DA-9601 plays a role in its protective mechanism(s), at least in part.

• Reproductive and Developmental Biology
• /
• v.34 no.3
• /
• pp.135-141
• /
• 2010

Embryoid bodies (EBs) generated from human embryonic stem cells (hESCs) include spontaneously induced endodermal lineage cells (ELCs). Activin-A plays important roles in the endoderm differentiation of hESCs. Despite studies on the generation of ELCs from hESCs with treatment of Actvin-A, it was unclear for localization and pattern of ELCs by Activin-A during differentiation of hESCs. Accordingly in this study, we knew that Actvin-A increased the cystic EBs formation, including the highly enriched AFP (endoderm lineage specific marker)-expressing cells in the surface of cystic EBs. To induce the EBs formation from undifferentiated hESCs, cells were transferred onto petri-dish and cultured in suspension condition without bFGF removed hESC media (EB media) for 3 days. Next to investigate the effect of Activin-A, EBs were subsequently cultured in EB media supplement with 100 ng/ml Activin-A for 3 days. After 5~7 days of Activin-A treatment, cystic EBs began to appear which increased in numbers reaching ~60% of initially formed EBs over 5 days. Endoderm lineage marker, AFP were highly expressed and specifically localized at the surface region of cystic EBs comparison with normal EBs. We next attached the cystic EBs onto gelatin-coated plates and cultured for 5 days. In the results of real-time PCR and immunocytochemistry analysis, AFP-expressing cells migrated and localized at the outgrowth region of attached cystic EBs. To obtain the AFP-expressing cells of the outgrowth region, we manually isolated by using micro-dissection and cultured them. These cells strongly express AFP over 70% of isolated cells post re-plating. Here, we first showed an expression pattern of specifically localized ELCs by Activin-A during differentiation of hESCs. From this observation, we could highly purified ELCs from undifferentiated hESCs. Taken together, our system will provide a novel and efficient option to generate ELCs from hESCs.

• Journal of Plant Biotechnology
• /
• v.32 no.1
• /
• pp.31-35
• /
• 2005

To obtain regeneration system of onion, we analyzed the effects of 2,4-D and BA concentration on the embryogenic callus induction from mature embryos. The highest embryogenic callus induction ratio was shown on MS medium (Murashie and Skoog 1962) containing $2.5\;\cal{mg/L}\;or\;5\;\cal{mg/L}$ picloram after mature embryos were placed on medium. When induced callus were cultured on half strength of MS medium containing $1\;\cal{mg/L}$ Kinetin, the highest shoot formation ratio was observed on MS medium containing $1\;{mg/L}$ 2,4-D and $1\;{mg/L}$ BA. Embryogenic callus were cultured in MS liquid medium containing $1\;\ccal{mg/L}$ of 2,4-D and $1\;\cal{mg/L}$ BA. The suspension cultured cell clumps could be mass propagated. Embryogenic callus were friable, but non-embryogenic callus included a lot of moisture, hence the identification between embryogenic and non-embryogenic callus as easily achieved. When embryogenic callus as cultured on half strength of MS medium containing $1\;\cal{mg/L}$ Kinetin, shoots were induced. The whole plantlet was obtained on rooting medium containing $0.5\;\cal{mg/}$ of NAA.

• Horticultural Science & Technology
• /
• v.28 no.6
• /
• pp.1072-1077
• /
• 2010

Plant diseases including gray mold caused by Botrytis cinerea are often reduced when calcium compounds are used as alternative materials in paprika. However, much less information is available about the effects of calcium compounds on controlling of $B.$ $cinerea$. Seven calcium compounds such as calcium sulfate dihydrate, calcium chloride, calcium nitrate, calcium oxide, calcium hydroxide, calcium carbonate, and calcium hydride were evaluated for their effectiveness against $B.$ $cinerea$ on potato dextrose agar medium. The pH of selected calcium compounds was higher (pH 8.2-10) than that of the control (pH 6.6). Calcium carbonate, calcium oxide, calcium hydride, and calcium hydroxide among seven calcium compounds were more effectively inhibited the growth of $B.$ $cinerea$ than other calcium compounds. In the case of spraying the spore suspension on paprika applied with the selected four calcium compounds and supplied with the selected calcium supplements in a hydroponic culture system, the paprika treated with calcium compounds showed less severity of disease than those untreated plants. On the basis of our results, we propose that the suppressive effects of calcium compounds on $B.$ $cinerea$ in paprika resulted from the supply of calcium and a certain degree of salt stress.

• Science of Emotion and Sensibility
• /
• v.13 no.2
• /
• pp.381-390
• /
• 2010

The automotive seat comfort evaluation was to begin a key aspect in seat design. It depended largely on the basic mechanical aspect such as geometric parameters of seat, choice of suspension system and cushion material used. Until recently, seat comfort evaluation advanced to evaluate subjective sensitivity of human. The external literatures showed in the last decade, there have been very few attempts to establish and document automotive seat comfort evaluation. In 2006 Smith, D. proposed the statistically reliable tool in giving a numeric rating for set comfort and the tool was used in the many country. On the other hands, we, in Korea, had not the reliable tool for the automotive seat comfort evaluation. So that, we studied to develope the questionnaire for seat comfort evaluation based on Smith, D.(2006) and some studies. As a result, we developed 36 contents for the automotive seat comfort evaluation with the help of professional in Korean automotive industry. Here, 36 contents were identified as the dimensions that represent the human sensitivity and psychological feeling on comfortable seat. Also, we derived the priorities for the 36 contents by using analytic hierarchy process (AHP), based on the judgments of 30 experts and drivers. This study will help the designers and developers clarify the conceptual and abstract aspect of the design evaluation by proposing a more systematic and process-oriented method.